The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro

DisqueKochem C, Dreiseikelmann B (1997)
JOURNAL OF BACTERIOLOGY 179(19): 6133-6137.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
Abstract / Bemerkung
The cytoplasmic protein TraM is one of four essential gene products of the F factor which are involved in DNA transfer after mating pair formation. TraM binds to three specific sites within the oriT region. Besides regulation of its own synthesis, the precise function of TraM during conjugation is not yet known. In the present work, the affinity of TraM to TraD was studied in vitro by an overlay assay and by affinity chromatography. Whether the interaction between TraM and TraD causes a transient or permanent anchoring of the F factor to the site of transfer is discussed, A 35-kDa host membrane protein of yet unknown function also shows affinity to TraM and may be involved in this anchoring process as well.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BACTERIOLOGY
Band
179
Zeitschriftennummer
19
Seite
6133-6137
ISSN
PUB-ID

Zitieren

DisqueKochem C, Dreiseikelmann B. The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY. 1997;179(19):6133-6137.
DisqueKochem, C., & Dreiseikelmann, B. (1997). The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY, 179(19), 6133-6137.
DisqueKochem, C., and Dreiseikelmann, B. (1997). The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY 179, 6133-6137.
DisqueKochem, C., & Dreiseikelmann, B., 1997. The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY, 179(19), p 6133-6137.
C. DisqueKochem and B. Dreiseikelmann, “The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro”, JOURNAL OF BACTERIOLOGY, vol. 179, 1997, pp. 6133-6137.
DisqueKochem, C., Dreiseikelmann, B.: The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY. 179, 6133-6137 (1997).
DisqueKochem, C, and Dreiseikelmann, Brigitte. “The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro”. JOURNAL OF BACTERIOLOGY 179.19 (1997): 6133-6137.

58 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Conjugative DNA Transfer Is Enhanced by Plasmid R1 Partitioning Proteins.
Gruber CJ, Lang S, Rajendra VK, Nuk M, Raffl S, Schildbach JF, Zechner EL., Front Mol Biosci 3(), 2016
PMID: 27486582
Towards an integrated model of bacterial conjugation.
Cabezón E, Ripoll-Rozada J, Peña A, de la Cruz F, Arechaga I., FEMS Microbiol Rev 39(1), 2015
PMID: 25154632
Mechanism and structure of the bacterial type IV secretion systems.
Christie PJ, Whitaker N, González-Rivera C., Biochim Biophys Acta 1843(8), 2014
PMID: 24389247
Common requirement for the relaxosome of plasmid R1 in multiple activities of the conjugative type IV secretion system.
Lang S, Gruber CJ, Raffl S, Reisner A, Zechner EL., J Bacteriol 196(11), 2014
PMID: 24682328
Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.
Wong JJ, Lu J, Edwards RA, Frost LS, Glover JN., Nucleic Acids Res 39(15), 2011
PMID: 21565799
An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation.
Lang S, Kirchberger PC, Gruber CJ, Redzej A, Raffl S, Zellnig G, Zangger K, Zechner EL., Mol Microbiol 82(5), 2011
PMID: 22066957
Conjugative DNA metabolism in Gram-negative bacteria.
de la Cruz F, Frost LS, Meyer RJ, Zechner EL., FEMS Microbiol Rev 34(1), 2010
PMID: 19919603
Functional dissection of the conjugative coupling protein TrwB.
de Paz HD, Larrea D, Zunzunegui S, Dehio C, de la Cruz F, Llosa M., J Bacteriol 192(11), 2010
PMID: 20363945
The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains.
Wittmann J, Eichenlaub R, Dreiseikelmann B., Microbiology 156(pt 8), 2010
PMID: 20447991
Mobility of plasmids.
Smillie C, Garcillán-Barcia MP, Francia MV, Rocha EP, de la Cruz F., Microbiol Mol Biol Rev 74(3), 2010
PMID: 20805406
Molecular recognition determinants for type IV secretion of diverse families of conjugative relaxases.
Lang S, Gruber K, Mihajlovic S, Arnold R, Gruber CJ, Steinlechner S, Jehl MA, Rattei T, Fröhlich KU, Zechner EL., Mol Microbiol 78(6), 2010
PMID: 21143323
A novel fold in the TraI relaxase-helicase c-terminal domain is essential for conjugative DNA transfer.
Guogas LM, Kennedy SA, Lee JH, Redinbo MR., J Mol Biol 386(2), 2009
PMID: 19136009
Plasmid r1 conjugative DNA processing is regulated at the coupling protein interface.
Mihajlovic S, Lang S, Sut MV, Strohmaier H, Gruber CJ, Koraimann G, Cabezón E, Moncalián G, de la Cruz F, Zechner EL., J Bacteriol 191(22), 2009
PMID: 19767437
Protein and DNA effectors control the TraI conjugative helicase of plasmid R1.
Sut MV, Mihajlovic S, Lang S, Gruber CJ, Zechner EL., J Bacteriol 191(22), 2009
PMID: 19767439
Biological diversity of prokaryotic type IV secretion systems.
Alvarez-Martinez CE, Christie PJ., Microbiol Mol Biol Rev 73(4), 2009
PMID: 19946141
Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediate.
Chen Y, Zhang X, Manias D, Yeo HJ, Dunny GM, Christie PJ., J Bacteriol 190(10), 2008
PMID: 18326569
Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation.
Lu J, Wong JJ, Edwards RA, Manchak J, Frost LS, Glover JN., Mol Microbiol 70(1), 2008
PMID: 18717787
The F plasmid-encoded TraM protein stimulates relaxosome-mediated cleavage at oriT through an interaction with TraI.
Ragonese H, Haisch D, Villareal E, Choi JH, Matson SW., Mol Microbiol 63(4), 2007
PMID: 17238924
In vivo oligomerization of the F conjugative coupling protein TraD.
Haft RJ, Gachelet EG, Nguyen T, Toussaint L, Chivian D, Traxler B., J Bacteriol 189(18), 2007
PMID: 17631633
Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM.
Lu J, Edwards RA, Wong JJ, Manchak J, Scott PG, Frost LS, Glover JN., EMBO J 25(12), 2006
PMID: 16710295
Cut and move: protein machinery for DNA processing in bacterial conjugation.
Gomis-Rüth FX, Coll M., Curr Opin Struct Biol 16(6), 2006
PMID: 17079132
The DotL protein, a member of the TraG-coupling protein family, is essential for Viability of Legionella pneumophila strain Lp02.
Buscher BA, Conover GM, Miller JL, Vogel SA, Meyers SN, Isberg RR, Vogel JP., J Bacteriol 187(9), 2005
PMID: 15838018
Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems.
Christie PJ., Biochim Biophys Acta 1694(1-3), 2004
PMID: 15546668
The outs and ins of bacterial type IV secretion substrates.
Ding Z, Atmakuri K, Christie PJ., Trends Microbiol 11(11), 2003
PMID: 14607070
TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates?
Schröder G, Krause S, Zechner EL, Traxler B, Yeo HJ, Lurz R, Waksman G, Lanka E., J Bacteriol 184(10), 2002
PMID: 11976307
Bacterial conjugation: a two-step mechanism for DNA transport.
Llosa M, Gomis-Rüth FX, Coll M, de la Cruz Fd F., Mol Microbiol 45(1), 2002
PMID: 12100543
Induction of a DNA nickase in the presence of its target site stimulates adaptive mutation in Escherichia coli.
Rodriguez C, Tompkin J, Hazel J, Foster PL., J Bacteriol 184(20), 2002
PMID: 12270817
Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates.
Disqué-Kochem C, Battermann A, Strätz M, Dreiseikelmann B., Microbiol Res 156(2), 2001
PMID: 11572456
Transfer protein TraY of plasmid R1 stimulates TraI-catalyzed oriT cleavage in vivo.
Karl W, Bamberger M, Zechner EL., J Bacteriol 183(3), 2001
PMID: 11208788
Mobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1.
Szpirer CY, Faelen M, Couturier M., J Bacteriol 183(6), 2001
PMID: 11222611
Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein Mob.
Szpirer CY, Faelen M, Couturier M., Mol Microbiol 37(6), 2000
PMID: 10998162
IHF protein inhibits cleavage but not assembly of plasmid R388 relaxosomes.
Moncalián G, Valle M, Valpuesta JM, de la Cruz F., Mol Microbiol 31(6), 1999
PMID: 10209739
Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.
Lee MH, Kosuk N, Bailey J, Traxler B, Manoil C., J Bacteriol 181(19), 1999
PMID: 10498725
Characterization of ATP and DNA binding activities of TrwB, the coupling protein essential in plasmid R388 conjugation.
Moncalián G, Cabezón E, Alkorta I, Valle M, Moro F, Valpuesta JM, Goñi FM, de La Cruz F., J Biol Chem 274(51), 1999
PMID: 10593894
The essential transfer protein TraM binds to DNA as a tetramer.
Verdino P, Keller W, Strohmaier H, Bischof K, Lindner H, Koraimann G., J Biol Chem 274(52), 1999
PMID: 10601314

27 References

Daten bereitgestellt von Europe PubMed Central.

Blotting and band-shifting: techniques for studying protein-protein interactions.
Carr DW, Scott JD., Trends Biochem. Sci. 17(7), 1992
PMID: 1323890
Enzymic unwinding of DNA. 2. Chain separation by an ATP-dependent DNA unwinding enzyme.
Abdel-Monem M, Durwald H, Hoffmann-Berling H., Eur. J. Biochem. 65(2), 1976
PMID: 133023
The TraM protein of the conjugative plasmid F binds to the origin of transfer of the F and ColE1 plasmids.
Di Laurenzio L, Frost LS, Paranchych W., Mol. Microbiol. 6(20), 1992
PMID: 1479887
Endonuclease activity of Escherichia coli DNA helicase I directed against the transfer origin of the F factor.
Reygers U, Wessel R, Muller H, Hoffmann-Berling H., EMBO J. 10(9), 1991
PMID: 1651233
Characterization of the oriT region of the IncFV plasmid pED208.
Di Laurenzio L, Frost LS, Finlay BB, Paranchych W., Mol. Microbiol. 5(7), 1991
PMID: 1943709
Deletion analysis of the F plasmid oriT locus.
Fu YH, Tsai MM, Luo YN, Deonier RC., J. Bacteriol. 173(3), 1991
PMID: 1991706
The TraM protein of plasmid R1 is a DNA-binding protein.
Schwab M, Gruber H, Hogenauer G., Mol. Microbiol. 5(2), 1991
PMID: 2041477
Intrinsic bends and integration host factor binding at F plasmid oriT.
Tsai MM, Fu YH, Deonier RC., J. Bacteriol. 172(8), 1990
PMID: 2198269
Purified Escherichia coli F-factor TraY protein binds oriT.
Lahue EE, Matson SW., J. Bacteriol. 172(3), 1990
PMID: 2407722
Nucleotide sequence of the traD region in the Escherichia coli F sex factor.
Jalajakumari MB, Manning PA., Gene 81(2), 1989
PMID: 2680768
The conjugation system of F, the fertility factor of Escherichia coli.
Ippen-Ihler KA, Minkley EG Jr., Annu. Rev. Genet. 20(), 1986
PMID: 2880557
Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector.
Furste JP, Pansegrau W, Frank R, Blocker H, Scholz P, Bagdasarian M, Lanka E., Gene 48(1), 1986
PMID: 3549457
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Towbin H, Staehelin T, Gordon J., Proc. Natl. Acad. Sci. U.S.A. 76(9), 1979
PMID: 388439
The conjugation system of F-like plasmids.
Willetts N, Skurray R., Annu. Rev. Genet. 14(), 1980
PMID: 6111288
Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ.
Thompson R, Taylor L., Mol. Gen. Genet. 188(3), 1982
PMID: 6298579
Analysis of the sequence and gene products of the transfer region of the F sex factor.
Frost LS, Ippen-Ihler K, Skurray RA., Microbiol. Rev. 58(2), 1994
PMID: 7915817
Translocation of DNA across bacterial membranes.
Dreiseikelmann B., Microbiol. Rev. 58(3), 1994
PMID: 7968916
Mutational and physical analysis of F plasmid traY protein binding to oriT.
Luo Y, Gao Q, Deonier RC., Mol. Microbiol. 11(3), 1994
PMID: 8152370
Characterization of the Escherichia coli F factor traY gene product and its binding sites.
Nelson WC, Morton BS, Lahue EE, Matson SW., J. Bacteriol. 175(8), 1993
PMID: 8468282
Regulation of the expression of the traM gene of the F sex factor of Escherichia coli.
Penfold SS, Simon J, Frost LS., Mol. Microbiol. 20(3), 1996
PMID: 8736534

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 9324263
PubMed | Europe PMC

Suchen in

Google Scholar