Mechanism of electroporative dye uptake by mouse B cells

Neumann E, Tönsing K, Kakorin S, Budde P, Frey J (1998)
BIOPHYSICAL JOURNAL 74(1): 98-108.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The color change of electroporated intact immunoglobulin G receptor(-) (Fc gamma R-) mouse B cells (line llA1.6) after direct electroporative transfer of the dye SERVA blue G (M-r 854) into the cell interior is shown to be dominantly due to diffusion of the dye after the electric field pulse. Hence the dye transport is described by Fick's first law, where, as a novelty, time-integrated flow coefficients are introduced. The chemical-kinetic analysis uses three different pore states (P) in the reaction cascade (C reversible arrow P-1 reversible arrow P-2 reversible arrow P-3), to model the sigmoid kinetics of pore formation as well as the biphasic pore resealing. The rate coefficient for pore formation k(p) is dependent on the external electric field strength E and pulse duration t(E). At E = 2.1 kV cm(-1) and t(E) = 200 mu s, k(p) = (2.4 +/- 0.2) x 10(3) s(-1) at T = 293 K; the respective (field-dependent) flow coefficient and permeability coefficient are k(f)(0) = (1.0 +/- 0.1) x 10(-2) s(-1) and P-0 = 2 cm s(-1), respectively. The maximum value of the fractional surface area of the dye-conductive pores is 0.035 +/- 0.003%, and the maximum pore number is N-p = (1.5 +/- 0.1) x 10(5) per average cell. The diffusion coefficient for SERVA blue G, D = 10(-6) cm(2) s(-1), is slightly smaller than that of free dye diffusion, indicating transient interaction of the dye with the pore lipids during translocation. The mean radii of the three pore states are (r) over bar(P-1) = 0.7 +/- 0.1 nm, (r) over bar(P-2) = 1.0 +/- 0.1 nm, and (r) over bar(P-3) = 1.2 +/- 0.1 nm, respectively. The resealing rate coefficients are k(-2) = (4.0 +/- 0.5) x 10(-2) s(-1) and k(-3) = (4.5 +/- 0.5) x 10(-3) s(-1), independent of E. At zero field, the equilibrium constant of the pore states (P) relative to closed membrane states (C) is K-p(0) = [(P)]/[C] = 0.02 +/- 0.002, indicating 2.0 +/- 0.2% water associated with the lipid membrane. Finally, the results of SERVA blue G cell coloring and the new analytical framework may also serve as a guideline for the optimization of the electroporative delivery of drugs that are similar in structure to SERVA blue G, for instance, bleomycin, which has been used successfully in the new discipline of electrochemotherapy.
Erscheinungsjahr
Zeitschriftentitel
BIOPHYSICAL JOURNAL
Band
74
Ausgabe
1
Seite(n)
98-108
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Neumann E, Tönsing K, Kakorin S, Budde P, Frey J. Mechanism of electroporative dye uptake by mouse B cells. BIOPHYSICAL JOURNAL. 1998;74(1):98-108.
Neumann, E., Tönsing, K., Kakorin, S., Budde, P., & Frey, J. (1998). Mechanism of electroporative dye uptake by mouse B cells. BIOPHYSICAL JOURNAL, 74(1), 98-108. doi:10.1016/S0006-3495(98)77771-9
Neumann, E., Tönsing, K., Kakorin, S., Budde, P., and Frey, J. (1998). Mechanism of electroporative dye uptake by mouse B cells. BIOPHYSICAL JOURNAL 74, 98-108.
Neumann, E., et al., 1998. Mechanism of electroporative dye uptake by mouse B cells. BIOPHYSICAL JOURNAL, 74(1), p 98-108.
E. Neumann, et al., “Mechanism of electroporative dye uptake by mouse B cells”, BIOPHYSICAL JOURNAL, vol. 74, 1998, pp. 98-108.
Neumann, E., Tönsing, K., Kakorin, S., Budde, P., Frey, J.: Mechanism of electroporative dye uptake by mouse B cells. BIOPHYSICAL JOURNAL. 74, 98-108 (1998).
Neumann, Eberhard, Tönsing, Katja, Kakorin, Sergej, Budde, P, and Frey, Jürgen. “Mechanism of electroporative dye uptake by mouse B cells”. BIOPHYSICAL JOURNAL 74.1 (1998): 98-108.

65 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation.
Rajeckaitė V, Jakštys B, Rafanavičius A, Maciulevičius M, Jakutavičiūtė M, Šatkauskas S., J Membr Biol 251(1), 2018
PMID: 29143077
Transport of charged small molecules after electropermeabilization - drift and diffusion.
Sözer EB, Pocetti CF, Vernier PT., BMC Biophys 11(), 2018
PMID: 29581879
Direct Current Helium Plasma for In vivo Delivery of Plasmid DNA Encoding Erythropoietin to Murine Skin.
Jaroszeski MJ, Harvey-Chapman T, Hoff A, Atkins R, Connolly RJ., Plasma Med 7(3), 2017
PMID: 30854158
Comparison between direct and reverse electroporation of cells in situ: a simulation study.
Towhidi L, Khodadadi D, Maimari N, Pedrigi RM, Ip H, Kis Z, Kwak BR, Petrova TW, Delorenzi M, Krams R., Physiol Rep 4(6), 2016
PMID: 27009275
Single-step electrical field strength screening to determine electroporation induced transmembrane transport parameters.
Blumrosen G, Abazari A, Golberg A, Yarmush ML, Toner M., Biochim Biophys Acta 1858(9), 2016
PMID: 27263825
Cell Monitoring and Manipulation Systems (CMMSs) based on Glass Cell-Culture Chips (GC³s).
Buehler SM, Stubbe M, Bonk SM, Nissen M, Titipornpun K, Klinkenberg ED, Baumann W, Gimsa J., Micromachines (Basel) 7(7), 2016
PMID: 30404280
Manufacturing Man-Made Magnetosomes: High-Throughput In Situ Synthesis of Biomimetic Magnetite Loaded Nanovesicles.
Bakhshi PK, Bain J, Gul MO, Stride E, Edirisinghe M, Staniland SS., Macromol Biosci 16(11), 2016
PMID: 27490757
Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation.
Venslauskas MS, Šatkauskas S., Eur Biophys J 44(5), 2015
PMID: 25939984
Single-cell juxtacellular transfection and recording technique.
Daniel J, Polder HR, Lessmann V, Brigadski T., Pflugers Arch 465(11), 2013
PMID: 23748581
Towards the mechanisms for efficient gene transfer into cells and tissues by means of cell electroporation.
Satkauskas S, Ruzgys P, Venslauskas MS., Expert Opin Biol Ther 12(3), 2012
PMID: 22339479
Carbon nanotube-mediated wireless cell permeabilization: drug and gene uptake.
Raffa V, Gherardini L, Vittorio O, Bardi G, Ziaei A, Pizzorusso T, Riggio C, Nitodas S, Karachalios T, Al-Jamal KT, Kostarelos K, Costa M, Cuschieri A., Nanomedicine (Lond) 6(10), 2011
PMID: 22122583
Characterization of plasma mediated molecular delivery to cells in vitro.
Connolly RJ, Lopez GA, Hoff AM, Jaroszeski MJ., Int J Pharm 389(1-2), 2010
PMID: 20083175
Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cells.
Ibey BL, Pakhomov AG, Gregory BW, Khorokhorina VA, Roth CC, Rassokhin MA, Bernhard JA, Wilmink GJ, Pakhomova ON., Biochim Biophys Acta 1800(11), 2010
PMID: 20691249
Transmembrane voltage analyses in spheroidal cells in response to an intense ultrashort electrical pulse.
Hu Q, Joshi RP., Phys Rev E Stat Nonlin Soft Matter Phys 79(1 pt 1), 2009
PMID: 19257063
Enhanced low voltage cell electropermeabilization by boron nitride nanotubes.
Raffa V, Ciofani G, Cuschieri A., Nanotechnology 20(7), 2009
PMID: 19417408
Rapid and efficient cell labeling with a MRI contrast agent by electroporation in the presence of protamine sulfate.
Wang L, Wang Z, Frank TG, Brown SI, Chudek SA, Cuschieri A., Nanomedicine (Lond) 4(3), 2009
PMID: 19331538
Control of the release of freely diffusing molecules in single-cell electroporation.
Agarwal A, Wang M, Olofsson J, Orwar O, Weber SG., Anal Chem 81(19), 2009
PMID: 19731948
Mechanistic analysis of electroporation-induced cellular uptake of macromolecules.
Zaharoff DA, Henshaw JW, Mossop B, Yuan F., Exp Biol Med (Maywood) 233(1), 2008
PMID: 18156311
Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex.
Kang JH, Toita R, Niidome T, Katayama Y., Cancer Lett 265(2), 2008
PMID: 18375054
Kinetics of transmembrane transport of small molecules into electropermeabilized cells.
Pucihar G, Kotnik T, Miklavcic D, Teissié J., Biophys J 95(6), 2008
PMID: 18539632
Phagocytic clearance of electric field induced 'apoptosis-mimetic' cells.
Tekle E, Wolfe MD, Oubrahim H, Chock PB., Biochem Biophys Res Commun 376(2), 2008
PMID: 18771656
Mass Transfer Model for Drug Delivery in Tissue Cells with Reversible Electroporation.
Granot Y, Rubinsky B., Int J Heat Mass Transf 51(23-24), 2008
PMID: 19884974
High electrical field effects on cell membranes.
Pliquett U, Joshi RP, Sridhara V, Schoenbach KH., Bioelectrochemistry 70(2), 2007
PMID: 17123870
Electropermeabilization of dense cell suspensions.
Pucihar G, Kotnik T, Teissié J, Miklavcic D., Eur Biophys J 36(3), 2007
PMID: 17294179
Calcium indicator loading of neurons using single-cell electroporation.
Nevian T, Helmchen F., Pflugers Arch 454(4), 2007
PMID: 17334778
Single-cell electroporation arrays with real-time monitoring and feedback control.
Khine M, Ionescu-Zanetti C, Blatz A, Wang LP, Lee LP., Lab Chip 7(4), 2007
PMID: 17389961
Effect of the intracellular localization of a Gd-based imaging probe on the relaxation enhancement of water protons.
Terreno E, Geninatti Crich S, Belfiore S, Biancone L, Cabella C, Esposito G, Manazza AD, Aime S., Magn Reson Med 55(3), 2006
PMID: 16450336
The tissue distribution of Evans blue dye in a sheep model of sentinel node biopsy.
Green M, Farshid G, Kollias J, Chatterton BE, Tsopelas C., Nucl Med Commun 27(9), 2006
PMID: 16894323
Effect of cell electroporation on the conductivity of a cell suspension.
Pavlin M, Kanduser M, Rebersek M, Pucihar G, Hart FX, Magjarevic R, Miklavcic D., Biophys J 88(6), 2005
PMID: 15792975
Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes.
Frantescu A, Kakorin S, Toensing K, Neumann E., Phys Chem Chem Phys 7(24), 2005
PMID: 16474877
Novel strategy to study gene expression and function in developing cerebellar granule cells.
Yang ZJ, Appleby VJ, Coyle B, Chan WI, Tahmaseb M, Wigmore PM, Scotting PJ., J Neurosci Methods 132(2), 2004
PMID: 14706712
Electroporation of subcutaneous mouse tumors by rectangular and trapezium high voltage pulses.
Pliquett U, Elez R, Piiper A, Neumann E., Bioelectrochemistry 62(1), 2004
PMID: 14990329
High yield electroextraction of proteins from yeast by a flow process.
Ganeva V, Galutzov B, Teissié J., Anal Biochem 315(1), 2003
PMID: 12672414
Impact of temperature on lethality and energy efficiency of apple juice pasteurization by pulsed electric fields treatment
Heinz V, Toepfl S, Knorr D., Innovative food science & emerging technologies : IFSET : the official scientific journal of the European Federation of Food Science and Technology. 4(2), 2003
PMID: IND44633177
Quantitative model of small molecules uptake after in vitro cell electropermeabilization.
Puc M, Kotnik T, Mir LM, Miklavcic D., Bioelectrochemistry 60(1-2), 2003
PMID: 12893304
Diverse effects of nanosecond pulsed electric fields on cells and tissues.
Beebe SJ, White J, Blackmore PF, Deng Y, Somers K, Schoenbach KH., DNA Cell Biol 22(12), 2003
PMID: 14683589
In vivo electroporation for genetic manipulations of whole Hydra polyps.
Bosch TC, Augustin R, Gellner K, Khalturin K, Lohmann JU., Differentiation 70(4-5), 2002
PMID: 12147133
Digression on membrane electroporation for drug and gene delivery.
Neumann E, Kakorin S., Technol Cancer Res Treat 1(5), 2002
PMID: 12625758
Highly efficient, large volume flow electroporation.
Li LH, Shivakumar R, Feller S, Allen C, Weiss JM, Dzekunov S, Singh V, Holaday J, Fratantoni J, Liu LN., Technol Cancer Res Treat 1(5), 2002
PMID: 12625759
Quantitative study of electroporation-mediated molecular uptake and cell viability.
Canatella PJ, Karr JF, Petros JA, Prausnitz MR., Biophys J 80(2), 2001
PMID: 11159443
Single-cell electroporation for gene transfer in vivo.
Haas K, Sin WC, Javaherian A, Li Z, Cline HT., Neuron 29(3), 2001
PMID: 11301019
Pulsed electric field breakage of cellular tissues: visualisation of percolative properties.
Lebovka NI, Bazhal MI, Vorobiev E., Innovative food science & emerging technologies : IFSET : the official scientific journal of the European Federation of Food Science and Technology. 2(2), 2001
PMID: IND23297796
Asymmetric pore distribution and loss of membrane lipid in electroporated DOPC vesicles.
Tekle E, Astumian RD, Friauf WA, Chock PB., Biophys J 81(2), 2001
PMID: 11463638
Perspectives for microelectrode arrays for biosensing and membrane electroporation.
Neumann E, Tönsing K, Siemens P., Bioelectrochemistry 51(2), 2000
PMID: 10910160
Effect of pH, ethanol addition and high hydrostatic pressure on the inactivation of Bacillus subtilis by pulsed electric fields.
Heinz V, Knorr D., Innovative food science & emerging technologies : IFSET : the official scientific journal of the European Federation of Food Science and Technology. 1(2), 2000
PMID: IND23291644
Gerhard schwarz: scientist and colleague
Neuman E, Winterhalter M., Biophys Chem 85(2-3), 2000
PMID: 10961499
Electroporation of curved lipid membranes in ionic strength gradients
Neumann E, Kakorin S., Biophys Chem 85(2-3), 2000
PMID: 10961510
Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy.
Ryttsén F, Farre C, Brennan C, Weber SG, Nolkrantz K, Jardemark K, Chiu DT, Orwar O., Biophys J 79(4), 2000
PMID: 11023903
Fundamentals of electroporative delivery of drugs and genes.
Neumann E, Kakorin S, Toensing K., Bioelectrochem Bioenerg 48(1), 1999
PMID: 10228565
Examination of the relationship between parameters to determine electropermeability of Saccharomyces cerevisiae.
Muraji M, Taniguchi H, Tatebe W, Berg H., Bioelectrochem Bioenerg 48(2), 1999
PMID: 10379573

41 References

Daten bereitgestellt von Europe PubMed Central.

Biological functions of human Fc gamma RIIa/Fc gamma RIIc in B cells.
Budde P, Bewarder N, Weinrich V, Frey J., Eur. J. Cell Biol. 64(1), 1994
PMID: 7957312
Membrane electroporation--fast molecular exchange by electroosmosis.
Dimitrov DS, Sowers AE., Biochim. Biophys. Acta 1022(3), 1990
PMID: 1690573
Antitumor electrochemotherapy: new advances in the clinical protocol.
Domenge C, Orlowski S, Luboinski B, De Baere T, Schwaab G, Belehradek J Jr, Mir LM., Cancer 77(5), 1996
PMID: 8608490

Eigen, 1963
Development of electrically enhanced drug delivery procedures for the treatment of melanoma and pancreatic cancer
Heller, 1993
Phase I/II trial for the treatment of cutaneous and subcutaneous tumors using electrochemotherapy.
Heller R, Jaroszeski MJ, Glass LF, Messina JL, Rapaport DP, DeConti RC, Fenske NA, Gilbert RA, Mir LM, Reintgen DS., Cancer 77(5), 1996
PMID: 8608491
Treatment of B16 mouse melanoma with the combination of electropermeabilization and chemotherapy
Heller, Bioelectrochem. Bioenerg. 36(), 1995
The effect of sodium ions on the electrical activity of giant axon of the squid.
HODGKIN AL, KATZ B., J. Physiol. (Lond.) 108(1), 1949
PMID: 18128147
Measurement of the hydrophobic interaction between two hydrophobic surfaces in aqueous electrolyte solutions
Israelachvili, J. Colloid Interface Sci. 98(), 1984
Electro-optics of membrane electroporation in diphenylhexatriene-doped lipid bilayer vesicles.
Kakorin S, Stoylov SP, Neumann E., Biophys. Chem. 58(1-2), 1996
PMID: 8679914
Survival of sucrose-loaded erythrocytes in the circulation.
Kinosita K Jr, Tsong TY., Nature 272(5650), 1978
PMID: 628451
Phospholipid hydration
McIntosh, 1993
Bleomycin: revival of an old drug.
Mir LM, Tounekti O, Orlowski S., Gen. Pharmacol. 27(5), 1996
PMID: 8842674
Induction of a long-lived fusogenic state in viable plant protoplasts permeabilized by electric fields.
Montane MH, Dupille E, Alibert G, Teissie J., Biochim. Biophys. Acta 1024(1), 1990
PMID: 2337618
The relaxation hysteresis of membrane electroporation
Neumann, 1989
Membrane electroporation and direct gene transfer
Neumann, Bioelectrochem. Bioenerg. 28(), 1992
Electric and magnetic field reception
Neumann, 1996
Membrane electroporation: biophysical and biotechnical aspects
Neumann, 1989
Membrane electroporation: the dye method to determine the cell membrane conductivity
Neumann, 1990
Electrooptics of membrane electroporation and vesicle shape deformation
Neumann, Curr. Opin. Colloid Interface Sci. 1(), 1996
Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation by electroporation.
Neumann E, Kakorin S, Tsoneva I, Nikolova B, Tomov T., Biophys. J. 71(2), 1996
PMID: 8842225
Gene transfer into mouse lyoma cells by electroporation in high electric fields.
Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH., EMBO J. 1(7), 1982
PMID: 6329708
Determination of the electrical field and anomalous heating caused by exponential pulses with aluminum electrodes in electroporation experiments
Pliquett, Bioelectrochem. Bioenerg. 39(), 1996
On dielectric relaxation due to chemical rate processes
Schwarz, J. Phys. Chem. 71(), 1967
Morphology of vesicles
Seifert, 1995
Antitumor effectiveness of electrochemotherapy with cis-diamminedichloroplatinum(II) in mice.
Sersa G, Cemazar M, Miklavcic D., Cancer Res. 55(15), 1995
PMID: 7614485
Electric field induced transient pores in phospholipid bilayer vesicles.
Teissie J, Tsong TY., Biochemistry 20(6), 1981
PMID: 6261800
Electro-permeabilization of cell membranes: effect of the resting membrane potential.
Tekle E, Astumian RD, Chock PB., Biochem. Biophys. Res. Commun. 172(1), 1990
PMID: 2222475
Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells.
Tekle E, Astumian RD, Chock PB., Proc. Natl. Acad. Sci. U.S.A. 88(10), 1991
PMID: 2034667
Selective and asymmetric molecular transport across electroporated cell membranes.
Tekle E, Astumian RD, Chock PB., Proc. Natl. Acad. Sci. U.S.A. 91(24), 1994
PMID: 7972093
Electroporation of cell membranes.
Tsong TY., Biophys. J. 60(2), 1991
PMID: 1912274
Factors affecting transdermal delivery of metoprolol by electroporation
Vanbever, Bioelectrochem. Bioenerg. 38(), 1995
Theory of electroporation: a review
Weaver, Bioelectrochem. Bioenerg. 41(), 1996

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