Fundamentals of electroporative delivery of drugs and genes

Neumann E, Kakorin S, Tönsing K (1999)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Electrooptical and conductometrical relaxation methods have given a new insight in the molecular mechanisms of the electroporative delivery of drug-like dyes and genes (DNA) to cells and tissues. Key findings are: (1) Membrane electroporation (ME) and hence the electroporative transmembrane transport of macromolecules are facilitated by a higher curvature of the membrane as well as by a gradient of the ionic strength across charged membranes, affecting the spontaneous curvature. (2) The degree of pore formation as the primary field response increases continuously without a threshold field strength, whereas secondary phenomena, such as a dramatic increase in the membrane permeability to drug-like dyes and DNA (also called electropermeabilization), indicate threshold field strength ranges. (3) The transfer of DNA by ME requires surface adsorption and surface insertion of the permeant molecule or part of it. The diffusion coefficient for the translocation of DNA (M-r approximate to 3.5 x 10(6)) through the electroporated membrane is D-m = 6.7 x 10(-13) cm(2) s(-1) and D-m for the drug-like dye Serva Blue G (M-r approximate to 854) is D-m = 2.0 x 10(-12) cm(2) s(-1). The slow electroporative transport of both DNA and drugs across the electroporated membrane reflects highly interactive (electro-) diffusion, involving many small pores coalesced into large, but transiently occluded pores (DNA). The data on mouse B-cells and yeast cells provide directly the flow and permeability coefficients of Serva blue G and plasmid DNA at different electroporation protocols. The physico-chemical theory of ME and electroporative transport in terms of time-dependent flow coefficients has been developed to such a degree that analytical expressions are available to handle curvature and ionic strength effects on ME and transport. The theory presents further useful tools for the optimization of the ME techniques in biotechnology and medicine, in particular in the new field of electroporative delivery of drugs (electrochemotherapy) and of DNA transfer and gene therapy. (C) 1999 Elsevier Science S.A. All rights reserved.
electrotransformation; electrochemotherapy; membrane; permeability; membrane adsorption; yeast cell; electroporation
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Neumann E, Kakorin S, Tönsing K. Fundamentals of electroporative delivery of drugs and genes. BIOELECTROCHEMISTRY AND BIOENERGETICS. 1999;48(1):3-16.
Neumann, E., Kakorin, S., & Tönsing, K. (1999). Fundamentals of electroporative delivery of drugs and genes. BIOELECTROCHEMISTRY AND BIOENERGETICS, 48(1), 3-16.
Neumann, Eberhard, Kakorin, Sergej, and Tönsing, Katja. 1999. “Fundamentals of electroporative delivery of drugs and genes”. BIOELECTROCHEMISTRY AND BIOENERGETICS 48 (1): 3-16.
Neumann, E., Kakorin, S., and Tönsing, K. (1999). Fundamentals of electroporative delivery of drugs and genes. BIOELECTROCHEMISTRY AND BIOENERGETICS 48, 3-16.
Neumann, E., Kakorin, S., & Tönsing, K., 1999. Fundamentals of electroporative delivery of drugs and genes. BIOELECTROCHEMISTRY AND BIOENERGETICS, 48(1), p 3-16.
E. Neumann, S. Kakorin, and K. Tönsing, “Fundamentals of electroporative delivery of drugs and genes”, BIOELECTROCHEMISTRY AND BIOENERGETICS, vol. 48, 1999, pp. 3-16.
Neumann, E., Kakorin, S., Tönsing, K.: Fundamentals of electroporative delivery of drugs and genes. BIOELECTROCHEMISTRY AND BIOENERGETICS. 48, 3-16 (1999).
Neumann, Eberhard, Kakorin, Sergej, and Tönsing, Katja. “Fundamentals of electroporative delivery of drugs and genes”. BIOELECTROCHEMISTRY AND BIOENERGETICS 48.1 (1999): 3-16.

177 Zitationen in Europe PMC

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Trezise AE., DNA Cell Biol 21(12), 2002
PMID: 12573047
Topical gene transfer into rat skin using electroporation.
Dujardin N, Van Deŕ Smissen P, Préat V., Pharm Res 18(1), 2001
PMID: 11336354
Single-cell electroporation for gene transfer in vivo.
Haas K, Sin WC, Javaherian A, Li Z, Cline HT., Neuron 29(3), 2001
PMID: 11301019
Muscle-specific enhancement of gene expression by incorporation of SV40 enhancer in the expression plasmid.
Li S, MacLaughlin FC, Fewell JG, Gondo M, Wang J, Nicol F, Dean DA, Smith LC., Gene Ther 8(6), 2001
PMID: 11313829
Anticytokine gene therapy of autoimmune diseases.
Prud'homme GJ, Lawson BR, Theofilopoulos AN., Expert Opin Biol Ther 1(3), 2001
PMID: 11727511
Pulsed electric field breakage of cellular tissues: visualisation of percolative properties.
Lebovka NI, Bazhal MI, Vorobiev E., Innovative food science & emerging technologies : IFSET : the official scientific journal of the European Federation of Food Science and Technology. 2(2), 2001
PMID: IND23297796
The influence of medium conductivity on electropermeabilization and survival of cells in vitro.
Pucihar G, Kotnik T, Kanduser M, Miklavcic D., Bioelectrochemistry 54(2), 2001
PMID: 11694390
Biophysical injury mechanisms in electrical shock trauma.
Lee RC, Zhang D, Hannig J., Annu Rev Biomed Eng 2(), 2000
PMID: 11701521
Amplifiable DNA from gram-negative and gram-positive bacteria by a low strength pulsed electric field method.
Vitzthum F, Geiger G, Bisswanger H, Elkine B, Brunner H, Bernhagen J., Nucleic Acids Res 28(8), 2000
PMID: 10734214
Perspectives for microelectrode arrays for biosensing and membrane electroporation.
Neumann E, Tönsing K, Siemens P., Bioelectrochemistry 51(2), 2000
PMID: 10910160
Gerhard schwarz: scientist and colleague
Neuman E, Winterhalter M., Biophys Chem 85(2-3), 2000
PMID: 10961499
Electroporation of curved lipid membranes in ionic strength gradients
Neumann E, Kakorin S., Biophys Chem 85(2-3), 2000
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Efficient nonviral cutaneous transfection.
Glasspool-Malone J, Somiari S, Drabick JJ, Malone RW., Mol Ther 2(2), 2000
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Theory and in vivo application of electroporative gene delivery.
Somiari S, Glasspool-Malone J, Drabick JJ, Gilbert RA, Heller R, Jaroszeski MJ, Malone RW., Mol Ther 2(3), 2000
PMID: 10985947
In vivo cell electrofusion.
Mekid H, Mir LM., Biochim Biophys Acta 1524(2-3), 2000
PMID: 11113558

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Molecular-basis for cell-membrane electroporation
Weaver, Ann. New York Acad. Sci. 720(), 1994
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Weaver, Biolectrochem. Bioenerg. 41(), 1996
Electrooptics of membrane electroporation and vesicle shape deformation
Neumann, Curr. Opin. Colloid. Interface Sci. 1(), 1996
Electro-optics of membrane electroporation in diphenylhexatriene-doped lipid bilayer vesicles.
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Annexin V and vesicle membrane electroporation.
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Membrane electrostatics
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Dip patch clamp currents suggest electrodiffusive transport of the polyelectrolyte DNA through lipid bilayers.
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Measurement of the hydrophobic interaction between two hydrophobic surfaces in aqueous electrolyte solutions
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Bleomycin: revival of an old drug.
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