Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells
Bartsch JW, Jackel M, Perz A, Jockusch H (2000)
FEBS letters 467(1): 123-127.
Zeitschriftenaufsatz
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Autor*in
Bartsch, JW;
Jackel, M;
Perz, A;
Jockusch, HaraldUniBi
Einrichtung
Abstract / Bemerkung
For reconstruction or repair of damaged tissues, an artificially regulated switch from proliferation to differentiation would be of great advantage. To achieve conditional myogenesis, we expressed MyoD in mouse C3H 10T1/2 fibroblastic cells, using a gene regulation system based on the synthetic steroid RU 486, BS stable co-transfection of a plasmid construct with the RU 486 dependent activator and an integrating inducible MyoD construct, a cell clone, designated 10T-RM, was obtained in which MyoD expression was stringently controlled by RU 486, 12 h after addition of 10 nM RU 486 to 10T-RM cells, saturation levels of MyoD mRNA were observed and greater than or equal to 2 days later, mRNA for embryonal myosin heavy chain (MyHC(emb)) was abundant and mononucleated cells fused into myotubes. (C) 2000 Federation of European Biochemical Societies.
Stichworte
C310T/12 mouse fibroblast;
gene expression;
myogenic factor;
MyoD myogenesis;
synthetic steroid RU 486;
inducible
Erscheinungsjahr
2000
Zeitschriftentitel
FEBS letters
Band
467
Ausgabe
1
Seite(n)
123-127
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1620663
Zitieren
Bartsch JW, Jackel M, Perz A, Jockusch H. Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters. 2000;467(1):123-127.
Bartsch, J. W., Jackel, M., Perz, A., & Jockusch, H. (2000). Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters, 467(1), 123-127. https://doi.org/10.1016/S0014-5793(00)01136-4
Bartsch, JW, Jackel, M, Perz, A, and Jockusch, Harald. 2000. “Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells”. FEBS letters 467 (1): 123-127.
Bartsch, J. W., Jackel, M., Perz, A., and Jockusch, H. (2000). Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters 467, 123-127.
Bartsch, J.W., et al., 2000. Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters, 467(1), p 123-127.
J.W. Bartsch, et al., “Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells”, FEBS letters, vol. 467, 2000, pp. 123-127.
Bartsch, J.W., Jackel, M., Perz, A., Jockusch, H.: Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters. 467, 123-127 (2000).
Bartsch, JW, Jackel, M, Perz, A, and Jockusch, Harald. “Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells”. FEBS letters 467.1 (2000): 123-127.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
2 Zitationen in Europe PMC
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