The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro

Horn S, Meyer J, Heukeshoven J, Fehse B, Schulze C, Li S, Frey J, Poll S, Stocking C, Jucker M (2001)
LEUKEMIA 15(1): 112-120.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The inositol polyphosphate 5-phosphatase SHIP plays an important role in negative signalling in B cells and mast cells and in the down-regulation of cytokine receptor-mediated signals in myeloid cells. SHIP is expressed as a 145 kDa full-length protein and an isoform of 135 kDa due to alternative splicing. Additional smaller forms of SHIP which are truncated at the carboxy terminus have been described in bone marrow and peripheral blood mononuclear cells (PBMC). Our data demonstrate that human bone marrow cells and PBMC from healthy donors and patients with acute myeloid leukemia express the 145 kDa form of SHIP and low amounts of a 135 kDa form of SHIP in vivo whereas C-terminal-truncated SHIP proteins are generated by a PMSF-sensitive protease during the preparation of cell lysates in vitro. We have further characterized this protease and identified a proteolytic cleavage site in the human SHIP protein C-terminal to tryptophan residue 941. These data support a physiological role for the 145 and 135 kDa forms of SHIP in bone marrow and peripheral blood cells from normal donors and patients with acute myeloid leukemia.
Stichworte
SHIP; acute myeloid leukemia; proteolysis
Erscheinungsjahr
2001
Zeitschriftentitel
LEUKEMIA
Band
15
Ausgabe
1
Seite(n)
112-120
ISSN
0887-6924
Page URI
https://pub.uni-bielefeld.de/record/1618024

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Horn S, Meyer J, Heukeshoven J, et al. The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro. LEUKEMIA. 2001;15(1):112-120.
Horn, S., Meyer, J., Heukeshoven, J., Fehse, B., Schulze, C., Li, S., Frey, J., et al. (2001). The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro. LEUKEMIA, 15(1), 112-120. doi:10.1038/sj.leu.2401990
Horn, S., Meyer, J., Heukeshoven, J., Fehse, B., Schulze, C., Li, S., Frey, J., Poll, S., Stocking, C., and Jucker, M. (2001). The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro. LEUKEMIA 15, 112-120.
Horn, S., et al., 2001. The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro. LEUKEMIA, 15(1), p 112-120.
S. Horn, et al., “The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro”, LEUKEMIA, vol. 15, 2001, pp. 112-120.
Horn, S., Meyer, J., Heukeshoven, J., Fehse, B., Schulze, C., Li, S., Frey, J., Poll, S., Stocking, C., Jucker, M.: The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro. LEUKEMIA. 15, 112-120 (2001).
Horn, S, Meyer, J, Heukeshoven, J, Fehse, B, Schulze, C, Li, S, Frey, Jürgen, Poll, S, Stocking, C, and Jucker, M. “The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro”. LEUKEMIA 15.1 (2001): 112-120.

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