Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin
Schwettmann L, Tschesche H (2001)
PROTEIN EXPRESSION AND PURIFICATION 21(1): 65-70.
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Autor*in
Schwettmann, L;
Tschesche, HaraldUniBi
Einrichtung
Abstract / Bemerkung
ADAM 9 is a member of the cellular metalloprotease/ disintegrin/cysteine-rich (MDC) gene family, related to soluble snake venom metalloproteases (SVMP). ADAMs may play important roles in cell-cell fusion, cell-matrix interaction, and other cellular functions. To investigate catalytic activity of human ADAM 9 we have cloned and expressed the metalloprotease domain of human ADAM 9 in Pichia pastoris. The recombinant protein was purified in a three-step purification procedure and activity was detected against gelatin, p-casein, and fibronectin. In addition we identified five normal and cancer cell lines expressing mRNA of human ADAM 9. (C) 2001 Academic Press.
Erscheinungsjahr
2001
Zeitschriftentitel
PROTEIN EXPRESSION AND PURIFICATION
Band
21
Ausgabe
1
Seite(n)
65-70
ISSN
1046-5928
Page URI
https://pub.uni-bielefeld.de/record/1617925
Zitieren
Schwettmann L, Tschesche H. Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin. PROTEIN EXPRESSION AND PURIFICATION. 2001;21(1):65-70.
Schwettmann, L., & Tschesche, H. (2001). Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin. PROTEIN EXPRESSION AND PURIFICATION, 21(1), 65-70. https://doi.org/10.1006/prep.2000.1374
Schwettmann, L, and Tschesche, Harald. 2001. “Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin”. PROTEIN EXPRESSION AND PURIFICATION 21 (1): 65-70.
Schwettmann, L., and Tschesche, H. (2001). Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin. PROTEIN EXPRESSION AND PURIFICATION 21, 65-70.
Schwettmann, L., & Tschesche, H., 2001. Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin. PROTEIN EXPRESSION AND PURIFICATION, 21(1), p 65-70.
L. Schwettmann and H. Tschesche, “Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin”, PROTEIN EXPRESSION AND PURIFICATION, vol. 21, 2001, pp. 65-70.
Schwettmann, L., Tschesche, H.: Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin. PROTEIN EXPRESSION AND PURIFICATION. 21, 65-70 (2001).
Schwettmann, L, and Tschesche, Harald. “Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin”. PROTEIN EXPRESSION AND PURIFICATION 21.1 (2001): 65-70.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
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