The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases

Tschesche H, Zolzer V, Triebel S, Bartsch S (2001)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Tschesche, HaraldUniBi; Zolzer, V; Triebel, S; Bartsch, S
Abstract / Bemerkung
The human neutrophil lipocalin (HNL), a member of the large family of lipocalins that exhibit various physiological functions, is coexpressed in granulocytes with progelatinase B (MMP-9). Part of it is covalently bound to the proenzyme and therefore may play a possible role in the activation process: of promatrix metalloproteinases. We now report that HNL is able to accelerate the direct activation of promatrix metalloproteinases slightly. A significant enhancement of the activity could be demonstrated for the HgCl2- and the plasma kallikrein-induced activation of all three secretory forms of proMMP-9 and of proMMP-8. The same activating effects were exerted by HNL isolated from granulocytes as well as by the recombinant forms expressed by the yeast Pichia pastoris or by Escherichia coli. This demonstrates that the carbohydrate moiety is not essential for the biological activity of HNL. Activation and activity enhancement are obviously mediated by entrapping the remaining N-terminal sequence residues of the partially truncated proenzyme into the hydrophobic binding pocket of the HNL. In conclusion these results document that HNL can exert an enzyme-activating effect in the regulation of inflammatory and pathophysiological responses of granulocytes in the physiological activation of MMPs that have been subject to limited proteolytic processing.
Pichia pastoris; lipocalin; activation; human leukocytes; matrix metalloproteinases
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Tschesche H, Zolzer V, Triebel S, Bartsch S. The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases. EUROPEAN JOURNAL OF BIOCHEMISTRY. 2001;268(7):1918-1928.
Tschesche, H., Zolzer, V., Triebel, S., & Bartsch, S. (2001). The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases. EUROPEAN JOURNAL OF BIOCHEMISTRY, 268(7), 1918-1928.
Tschesche, Harald, Zolzer, V, Triebel, S, and Bartsch, S. 2001. “The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases”. EUROPEAN JOURNAL OF BIOCHEMISTRY 268 (7): 1918-1928.
Tschesche, H., Zolzer, V., Triebel, S., and Bartsch, S. (2001). The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases. EUROPEAN JOURNAL OF BIOCHEMISTRY 268, 1918-1928.
Tschesche, H., et al., 2001. The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases. EUROPEAN JOURNAL OF BIOCHEMISTRY, 268(7), p 1918-1928.
H. Tschesche, et al., “The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases”, EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 268, 2001, pp. 1918-1928.
Tschesche, H., Zolzer, V., Triebel, S., Bartsch, S.: The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases. EUROPEAN JOURNAL OF BIOCHEMISTRY. 268, 1918-1928 (2001).
Tschesche, Harald, Zolzer, V, Triebel, S, and Bartsch, S. “The human neutrophil lipocalin supports the allosteric activation of matrix metalloproteinases”. EUROPEAN JOURNAL OF BIOCHEMISTRY 268.7 (2001): 1918-1928.

65 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Friend or foe? The dual role of neutrophils in lung injury and repair.
Grudzinska FS, Sapey E., Thorax 73(4), 2018
PMID: 29382796
Neutrophils Release Metalloproteinases during Adhesion in the Presence of Insulin, but Cathepsin G in the Presence of Glucagon.
Fedorova NV, Ksenofontov AL, Serebryakova MV, Stadnichuk VI, Gaponova TV, Baratova LA, Sud'ina GF, Galkina SI., Mediators Inflamm 2018(), 2018
PMID: 29670459
N-Glycosylation of Lipocalin 2 Is Not Required for Secretion or Exosome Targeting.
Borkham-Kamphorst E, Van de Leur E, Meurer SK, Buhl EM, Weiskirchen R., Front Pharmacol 9(), 2018
PMID: 29755357
Sub-Cellular Localization of Metalloproteinases in Megakaryocytes.
Malara A, Ligi D, Di Buduo CA, Mannello F, Balduini A., Cells 7(7), 2018
PMID: 30037039
Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats.
Abdollahi M, Ng TS, Rezaeizadeh A, Aamidor S, Twigg SM, Min D, McLennan SV., PLoS One 12(2), 2017
PMID: 28182694
Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion.
Chung IH, Wu TI, Liao CJ, Hu JY, Lin YH, Tai PJ, Lai CH, Lin KH., Oncotarget 7(10), 2016
PMID: 26840566
iTRAQ-Based Proteomics Screen identifies LIPOCALIN-2 (LCN-2) as a potential biomarker for colonic lateral-spreading tumors.
Wang X, Li A, Guo Y, Wang Y, Zhao X, Xiang L, Han Z, Li Y, Xu W, Zhuang K, Yan Q, Zhong J, Xiong J, Liu S., Sci Rep 6(), 2016
PMID: 27339395
Pathobiology of neutrophil-epithelial interactions.
Brazil JC, Parkos CA., Immunol Rev 273(1), 2016
PMID: 27558330
Diverse functional roles of lipocalin-2 in the central nervous system.
Jha MK, Lee S, Park DH, Kook H, Park KG, Lee IK, Suk K., Neurosci Biobehav Rev 49(), 2015
PMID: 25511817
Thyroid hormone-mediated regulation of lipocalin 2 through the Met/FAK pathway in liver cancer.
Chung IH, Chen CY, Lin YH, Chi HC, Huang YH, Tai PJ, Liao CJ, Tsai CY, Lin SL, Wu MH, Chen CY, Lin KH., Oncotarget 6(17), 2015
PMID: 25940797
Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients.
Balakrishnan L, Bhattacharjee M, Ahmad S, Nirujogi RS, Renuse S, Subbannayya Y, Marimuthu A, Srikanth SM, Raju R, Dhillon M, Kaur N, Jois R, Vasudev V, Ramachandra Y, Sahasrabuddhe NA, Prasad TsK, Mohan S, Gowda H, Shankar S, Pandey A., Clin Proteomics 11(1), 2014
PMID: 24393543
Adipokines as potential biomarkers in rheumatoid arthritis.
Del Prete A, Salvi V, Sozzani S., Mediators Inflamm 2014(), 2014
PMID: 24799765
Lipocalin-2 protein deficiency ameliorates experimental autoimmune encephalomyelitis: the pathogenic role of lipocalin-2 in the central nervous system and peripheral lymphoid tissues.
Nam Y, Kim JH, Seo M, Kim JH, Jin M, Jeon S, Seo JW, Lee WH, Bing SJ, Jee Y, Lee WK, Park DH, Kook H, Suk K., J Biol Chem 289(24), 2014
PMID: 24808182
Neutrophil gelatinase B-associated lipocalin and matrix metalloproteinase-9 complex as a surrogate serum marker of mucosal healing in ulcerative colitis.
de Bruyn M, Arijs I, Wollants WJ, Machiels K, Van Steen K, Van Assche G, Ferrante M, Rutgeerts P, Vermeire S, Opdenakker G., Inflamm Bowel Dis 20(7), 2014
PMID: 24871805
Expression and complex formation of MMP9, MMP2, NGAL, and TIMP1 in porcine myocardium but not in skeletal muscles in male pigs with tachycardia-induced systolic heart failure.
Kiczak L, Tomaszek A, Bania J, Paslawska U, Zacharski M, Noszczyk-Nowak A, Janiszewski A, Skrzypczak P, Ardehali H, Jankowska EA, Ponikowski P., Biomed Res Int 2013(), 2013
PMID: 23710440
Lipocalin-2 elicited by advanced glycation end-products promotes the migration of vascular smooth muscle cells.
Chung TW, Choi HJ, Kim CH, Jeong HS, Ha KT., Biochim Biophys Acta 1833(12), 2013
PMID: 24149112
Matrix metalloproteinase-9: Many shades of function in cardiovascular disease.
Yabluchanskiy A, Ma Y, Iyer RP, Hall ME, Lindsey ML., Physiology (Bethesda) 28(6), 2013
PMID: 24186934
Association of cellular and molecular responses in the rat mammary gland to 17β-estradiol with susceptibility to mammary cancer.
Ding L, Zhao Y, Warren CL, Sullivan R, Eliceiri KW, Shull JD., BMC Cancer 13(), 2013
PMID: 24304664
Matrix metalloproteinases in kidney homeostasis and diseases.
Tan RJ, Liu Y., Am J Physiol Renal Physiol 302(11), 2012
PMID: 22492945
The association between the preoperative serum levels of lipocalin-2 and matrix metalloproteinase-9 (MMP-9) and prognosis of breast cancer.
Sung H, Choi JY, Lee SA, Lee KM, Han S, Jeon S, Song M, Lee Y, Park SK, Yoo KY, Noh DY, Ahn SH, Kang D., BMC Cancer 12(), 2012
PMID: 22640376
No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer.
Cramer EP, Glenthøj A, Häger M, Juncker-Jensen A, Engelholm LH, Santoni-Rugiu E, Lund LR, Laerum OD, Cowland JB, Borregaard N., PLoS One 7(6), 2012
PMID: 22737251
Lipocalin2 promotes invasion, tumorigenicity and gemcitabine resistance in pancreatic ductal adenocarcinoma.
Leung L, Radulovich N, Zhu CQ, Organ S, Bandarchi B, Pintilie M, To C, Panchal D, Tsao MS., PLoS One 7(10), 2012
PMID: 23056397
Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics.
te Boekhorst BC, Bovens SM, Hellings WE, van der Kraak PH, van de Kolk KW, Vink A, Moll FL, van Oosterhout MF, de Vries JP, Doevendans PA, Goumans MJ, de Kleijn DP, van Echteld CJ, Pasterkamp G, Sluijter JP., Cardiovasc Res 89(3), 2011
PMID: 21030534
Regulation of matrix metalloproteinase activity in health and disease.
Hadler-Olsen E, Fadnes B, Sylte I, Uhlin-Hansen L, Winberg JO., FEBS J 278(1), 2011
PMID: 21087458
Nitroglycerin alters matrix remodeling proteins in THP-1 human macrophages and plasma metalloproteinase activity in rats.
Krishnatry AS, Fung SM, Brazeau DA, Soda D, Fung HL., Nitric Oxide 24(2), 2011
PMID: 21156214
Relationships of lipocalin 2 with breast tumorigenesis and metastasis.
Leng X, Wu Y, Arlinghaus RB., J Cell Physiol 226(2), 2011
PMID: 20857428
Adipokines: biofactors from white adipose tissue. A complex hub among inflammation, metabolism, and immunity.
Conde J, Scotece M, Gómez R, López V, Gómez-Reino JJ, Lago F, Gualillo O., Biofactors 37(6), 2011
PMID: 22038756
Implication of granulocyte-macrophage colony-stimulating factor induced neutrophil gelatinase-associated lipocalin in pathogenesis of rheumatoid arthritis revealed by proteome analysis.
Katano M, Okamoto K, Arito M, Kawakami Y, Kurokawa MS, Suematsu N, Shimada S, Nakamura H, Xiang Y, Masuko K, Nishioka K, Yudoh K, Kato T., Arthritis Res Ther 11(1), 2009
PMID: 20527084
Biological and pathobiological functions of gelatinase dimers and complexes.
Malla N, Sjøli S, Winberg JO, Hadler-Olsen E, Uhlin-Hansen L., Connect Tissue Res 49(3), 2008
PMID: 18661338
Interaction of pro-matrix metalloproteinase-9/proteoglycan heteromer with gelatin and collagen.
Malla N, Berg E, Uhlin-Hansen L, Winberg JO., J Biol Chem 283(20), 2008
PMID: 18359769
Construction of mathematical model for high-level expression of foreign genes in pPIC9 vector and its verification.
Wu B, Cha L, Du Z, Ying X, Li H, Xu L, Zheng X, Li E, Li W., Biochem Biophys Res Commun 354(2), 2007
PMID: 17239823
A novel alternative spliced variant of neutrophil gelatinase-associated lipocalin receptor in oesophageal carcinoma cells.
Fang WK, Xu LY, Lu XF, Liao LD, Cai WJ, Shen ZY, Li EM., Biochem J 403(2), 2007
PMID: 17253959
Biochemical and enzymatic characterization of purified covalent complexes of matrix metalloproteinase-9 and haptoglobin released by bovine granulocytes in vitro.
Bannikov GA, Mattoon JS, Abrahamsen EJ, Premanandan C, Green-Church KB, Marsh AE, Lakritz J., Am J Vet Res 68(9), 2007
PMID: 17764415
Co-expression by keratinocytes of migration stimulating factor (MSF) and a functional inhibitor of its bioactivity (MSFI).
Jones SJ, Florence MM, Ellis IR, Kankova K, Schor SL, Schor AM., Exp Cell Res 313(20), 2007
PMID: 17949711
Expression of neutrophil gelatinase-associated lipocalin in atherosclerosis and myocardial infarction.
Hemdahl AL, Gabrielsen A, Zhu C, Eriksson P, Hedin U, Kastrup J, Thorén P, Hansson GK., Arterioscler Thromb Vasc Biol 26(1), 2006
PMID: 16254208
Induction of neutrophil gelatinase-associated lipocalin in vascular injury via activation of nuclear factor-kappaB.
Bu DX, Hemdahl AL, Gabrielsen A, Fuxe J, Zhu C, Eriksson P, Yan ZQ., Am J Pathol 169(6), 2006
PMID: 17148685
Induction of matrix metalloproteinase-9 in mice during Toxocara canis larvae migration.
Lai SC, Chen KM, Chen HC, Lee HH., Parasitol Res 95(3), 2005
PMID: 15645290
Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
Simpson JL, Scott RJ, Boyle MJ, Gibson PG., Am J Respir Crit Care Med 172(5), 2005
PMID: 15901607
Resistance and susceptibility to tuberculosis analysed at the transcriptome level: lessons from mouse macrophages.
Keller C, Lauber J, Blumenthal A, Buer J, Ehlers S., Tuberculosis (Edinb) 84(3-4), 2004
PMID: 15207484
Beta-catenin regulates the gene of MMP-26, a novel metalloproteinase expressed both in carcinomas and normal epithelial cells.
Marchenko ND, Marchenko GN, Weinreb RN, Lindsey JD, Kyshtoobayeva A, Crawford HC, Strongin AY., Int J Biochem Cell Biol 36(5), 2004
PMID: 15006646
Interferon-beta prevents cytokine-induced neutrophil infiltration and attenuates blood-brain barrier disruption.
Veldhuis WB, Floris S, van der Meide PH, Vos IM, de Vries HE, Dijkstra CD, Bär PR, Nicolay K., J Cereb Blood Flow Metab 23(9), 2003
PMID: 12973022
Neutrophil-derived matrix metalloproteinase-9 is increased in severe asthma and poorly inhibited by glucocorticoids.
Cundall M, Sun Y, Miranda C, Trudeau JB, Barnes S, Wenzel SE., J Allergy Clin Immunol 112(6), 2003
PMID: 14657859
The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition.
Goetz DH, Holmes MA, Borregaard N, Bluhm ME, Raymond KN, Strong RK., Mol Cell 10(5), 2002
PMID: 12453412

44 References

Daten bereitgestellt von Europe PubMed Central.

Structure and sequence relationships in the lipocalins and related proteins.
Flower DR, North AC, Attwood TK., Protein Sci. 2(5), 1993
PMID: 7684291
The lipocalin protein family: a role in cell regulation.
Flower DR., FEBS Lett. 354(1), 1994
PMID: 7957904
Three odorant-binding proteins from rabbit nasal mucosa.
Garibotti M, Navarrini A, Pisanelli AM, Pelosi P., Chem. Senses 22(4), 1997
PMID: 9279461
Accumulation of apolipoproteins in the regenerating and remyelating mammalian peripheral nerve. Identification of apolipoprotein D, apolipoprotein A-IV, apolipoprotein E, and apolipoprotein A-I
Boyles, J. Biol. Chem. 265(), 1990
Possible pheromone-carrier function of two lipocalin proteins in the vomeronasal organ.
Miyawaki A, Matsushita F, Ryo Y, Mikoshiba K., EMBO J. 13(24), 1994
PMID: 7813422
cDNA sequence and expression pattern of the putative pheromone carrier aphrodisin.
Magert HJ, Hadrys T, Cieslak A, Groger A, Feller S, Forssmann WG., Proc. Natl. Acad. Sci. U.S.A. 92(6), 1995
PMID: 7892229
Arachidonic acid binds to apolipoprotein D: implications for the protein's function.
Morais Cabral JH, Atkins GL, Sanchez LM, Lopez-Boado YS, Lopez-Otin C, Sawyer L., FEBS Lett. 366(1), 1995
PMID: 7789516
Isolation of an adhesion-mediating protein from chick neural retina adherons.
Schubert D, LaCorbiere M., J. Cell Biol. 101(3), 1985
PMID: 2993313
A chick neural retina adhesion and survival molecule is a retinol-binding protein.
Schubert D, LaCorbiere M, Esch F., J. Cell Biol. 102(6), 1986
PMID: 3754874
The crystal structure of nitrophorin 4 at 1.5 A resolution: transport of nitric oxide by a lipocalin-based heme protein.
Andersen JF, Weichsel A, Balfour CA, Champagne DE, Montfort WR., Structure 6(10), 1998
PMID: 9782054
Purification and characterization of rat brain prostaglandin D synthetase.
Urade Y, Fujimoto N, Hayaishi O., J. Biol. Chem. 260(23), 1985
PMID: 3930495
A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase.
Triebel S, Blaser J, Reinke H, Tschesche H., FEBS Lett. 314(3), 1992
PMID: 1281792
Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase.
Kjeldsen L, Johnsen AH, Sengelov H, Borregaard N., J. Biol. Chem. 268(14), 1993
PMID: 7683678
The monitoring of inflammation by specific cellular markers.
Venge P., Scand. J. Clin. Lab. Invest. Suppl. 219(), 1994
PMID: 7701241
Leukocyte activation detected by increased plasma levels of inflammatory mediators in patients with ischemic cerebrovascular diseases.
Elneihoum AM, Falke P, Axelsson L, Lundberg E, Lindgarde F, Ohlsson K., Stroke 27(10), 1996
PMID: 8841320
Leukocyte activation in atherosclerosis: correlation with risk factors.
Elneihoum AM, Falke P, Hedblad B, Lindgarde F, Ohlsson K., Atherosclerosis 131(1), 1997
PMID: 9180248

Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils.
Sengelov H, Boulay F, Kjeldsen L, Borregaard N., Biochem. J. 299 ( Pt 2)(), 1994
PMID: 8172608
Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases.
Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L., Gut 38(3), 1996
PMID: 8675096
Latent collagenase and gelatinase from human neutrophils and their activation.
Tschesche H, Knauper V, Kramer S, Michaelis J, Oberhoff R, Reinke H., Matrix Suppl 1(), 1992
PMID: 1480034
A sandwich enzyme immunoassay for the determination of neutrophil lipocalin in body fluids.
Blaser J, Triebel S, Tschesche H., Clin. Chim. Acta 235(2), 1995
PMID: 7554268
Synthetic substrates for vertebrate collagenase.
Masui Y, Takemoto T, Sakakibara S, Hori H, Nagai Y., Biochem Med 17(2), 1977
PMID: 193489
The reliability of molecular weight determination by sulfate-polyacrylamide gel electrophoresis
Weber, J. Biol. Chem. 244(), 1968
Mercurial activation of human PMN leucocyte type IV procollagenase (gelatinase).
Triebel S, Blaser J, Reinke H, Knauper V, Tschesche H., FEBS Lett. 298(2-3), 1992
PMID: 1312026
Mercurial activation of human polymorphonuclear leucocyte procollagenase.
Blaser J, Knauper V, Osthues A, Reinke H, Tschesche H., Eur. J. Biochem. 202(3), 1991
PMID: 1662606
Molecular cloning and expression of a cDNA encoding NGAL: a lipocalin expressed in human neutrophils.
Bundgaard JR, Sengelov H, Borregaard N, Kjeldsen L., Biochem. Biophys. Res. Commun. 202(3), 1994
PMID: 8060329
Human brain prostaglandin D synthase has been evolutionarily differentiated from lipophilic-ligand carrier proteins.
Nagata A, Suzuki Y, Igarashi M, Eguchi N, Toh H, Urade Y, Hayaishi O., Proc. Natl. Acad. Sci. U.S.A. 88(9), 1991
PMID: 1902577
Glutathione-independent prostaglandin D synthase as a lead molecule for designing new functional proteins.
Toh H, Kubodera H, Nakajima N, Sekiya T, Eguchi N, Tanaka T, Urade Y, Hayaishi O., Protein Eng. 9(12), 1996
PMID: 9010921
Xanthophyll cycle enzymes are members of the lipocalin family, the first identified from plants.
Bugos RC, Hieber AD, Yamamoto HY., J. Biol. Chem. 273(25), 1998
PMID: 9624110
The cysteine switch: a principle of regulation of metalloproteinase activity with potential applicability to the entire matrix metalloproteinase gene family
Van, Proc. Natl Acad. Sci. USA 87(), 1990
Proteolytic and non-proteolytic activation of human neutrophil progelatinase B.
Sang QX, Birkedal-Hansen H, Van Wart HE., Biochim. Biophys. Acta 1251(2), 1995
PMID: 7669817
The solution structure and dynamics of human neutrophil gelatinase-associated lipocalin
Coles, J. Mol. Biol. 289(), 1999
Ligand preference inferred from the structure of neutrophil gelatinase associated lipocalin.
Goetz DH, Willie ST, Armen RS, Bratt T, Borregaard N, Strong RK., Biochemistry 39(8), 2000
PMID: 10684642

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