The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein

Niemeyer D, Becker A (2001)
JOURNAL OF BACTERIOLOGY 183(17): 5163-5170.

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Autor*in
Niemeyer, D; Becker, A
Abstract / Bemerkung
It is thought that in the gram-negative soil bacterium Sinorhizobium meliloti the protein ExoP is involved in biosynthesis of the acidic exopolysaccharide succinoglycan (EPS I). The amounts and compositions of EPS I produced by mutants expressing ExoP proteins characterized by specific amino acid substitutions in the C-terminal cytoplasmic domain were analyzed. The cytoplasmic domain of the ExoP protein was shown to have ATPase activity. Mutations in the highly conserved Walker A ATP-binding motif prevented ATPase activity of the ExoP protein. Phenotypically, these mutations resulted in much lower levels of succinoglycan which consisted only of monomers of the octasaccharide repeating unit. The ExoP protein has similarities to proteins with autophosphorylating protein tyrosine kinase activity. We found that ExoP was phosphorylated on tyrosine and that site-directed mutagenesis of specific tyrosine residues in the cytoplasmic domain of ExoP resulted in an altered ratio of low-molecular-weight succinoglycan to high-molecular-weight succinoglycan.
Erscheinungsjahr
2001
Zeitschriftentitel
JOURNAL OF BACTERIOLOGY
Band
183
Ausgabe
17
Seite(n)
5163-5170
ISSN
0021-9193
Page URI
https://pub.uni-bielefeld.de/record/1616604

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Niemeyer D, Becker A. The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein. JOURNAL OF BACTERIOLOGY. 2001;183(17):5163-5170.
Niemeyer, D., & Becker, A. (2001). The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein. JOURNAL OF BACTERIOLOGY, 183(17), 5163-5170. https://doi.org/10.1128/JB.183.17.5163-5170.2001
Niemeyer, D, and Becker, A. 2001. “The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein”. JOURNAL OF BACTERIOLOGY 183 (17): 5163-5170.
Niemeyer, D., and Becker, A. (2001). The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein. JOURNAL OF BACTERIOLOGY 183, 5163-5170.
Niemeyer, D., & Becker, A., 2001. The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein. JOURNAL OF BACTERIOLOGY, 183(17), p 5163-5170.
D. Niemeyer and A. Becker, “The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein”, JOURNAL OF BACTERIOLOGY, vol. 183, 2001, pp. 5163-5170.
Niemeyer, D., Becker, A.: The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein. JOURNAL OF BACTERIOLOGY. 183, 5163-5170 (2001).
Niemeyer, D, and Becker, A. “The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein”. JOURNAL OF BACTERIOLOGY 183.17 (2001): 5163-5170.

48 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Adenylate kinase potentiates the capsular polysaccharide by modulating Cps2D in Streptococcus pneumoniae D39.
Ghosh P, Luong TT, Shah M, Thach TT, Choi S, Lee S, Rhee DK., Exp Mol Med 50(9), 2018
PMID: 30185778
Synthesis of Rhizobial Exopolysaccharides and Their Importance for Symbiosis with Legume Plants.
Marczak M, Mazur A, Koper P, Żebracki K, Skorupska A., Genes (Basel) 8(12), 2017
PMID: 29194398
Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.
Schmid J, Sieber V, Rehm B., Front Microbiol 6(), 2015
PMID: 26074894
Autophosphorylation of the Bacterial Tyrosine-Kinase CpsD Connects Capsule Synthesis with the Cell Cycle in Streptococcus pneumoniae.
Nourikyan J, Kjos M, Mercy C, Cluzel C, Morlot C, Noirot-Gros MF, Guiral S, Lavergne JP, Veening JW, Grangeasse C., PLoS Genet 11(9), 2015
PMID: 26378458
Microbial protein-tyrosine kinases.
Chao JD, Wong D, Av-Gay Y., J Biol Chem 289(14), 2014
PMID: 24554699
Synthesis and assembly of a novel glycan layer in Myxococcus xanthus spores.
Holkenbrink C, Hoiczyk E, Kahnt J, Higgs PI., J Biol Chem 289(46), 2014
PMID: 25271164
Tyrosine phosphorylation and bacterial virulence.
Whitmore SE, Lamont RJ., Int J Oral Sci 4(1), 2012
PMID: 22388693
Bacterial tyrosine kinases: evolution, biological function and structural insights.
Grangeasse C, Nessler S, Mijakovic I., Philos Trans R Soc Lond B Biol Sci 367(1602), 2012
PMID: 22889913
Sequence-structure relationships in polysaccharide co-polymerase (PCP) proteins.
Morona R, Purins L, Tocilj A, Matte A, Cygler M., Trends Biochem Sci 34(2), 2009
PMID: 19058968
Tyrosine-kinases in bacteria: from a matter of controversy to the status of key regulatory enzymes.
Bechet E, Guiral S, Torres S, Mijakovic I, Cozzone AJ, Grangeasse C., Amino Acids 37(3), 2009
PMID: 19189200
Bacterial tyrosine kinases: novel targets for antibacterial therapy?
Cozzone AJ., Trends Microbiol 17(12), 2009
PMID: 19853456
Occurrence, production, and applications of gellan: current state and perspectives.
Fialho AM, Moreira LM, Granja AT, Popescu AO, Hoffmann K, Sá-Correia I., Appl Microbiol Biotechnol 79(6), 2008
PMID: 18506441
Structural basis for the regulation mechanism of the tyrosine kinase CapB from Staphylococcus aureus.
Olivares-Illana V, Meyer P, Bechet E, Gueguen-Chaignon V, Soulat D, Lazereg-Riquier S, Mijakovic I, Deutscher J, Cozzone AJ, Laprévote O, Morera S, Grangeasse C, Nessler S., PLoS Biol 6(6), 2008
PMID: 18547145
Influence of tyrosine-kinase Wzc activity on colanic acid production in Escherichia coli K12 cells.
Obadia B, Lacour S, Doublet P, Baubichon-Cortay H, Cozzone AJ, Grangeasse C., J Mol Biol 367(1), 2007
PMID: 17254603
UDP-acetyl-mannosamine dehydrogenase is an endogenous protein substrate of Staphylococcus aureus protein-tyrosine kinase activity.
Soulat D, Grangeasse C, Vaganay E, Cozzone AJ, Duclos B., J Mol Microbiol Biotechnol 13(1-3), 2007
PMID: 17693712
Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.
Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M., Proteomics 7(19), 2007
PMID: 17726680
Rhizobial exopolysaccharides: genetic control and symbiotic functions.
Skorupska A, Janczarek M, Marczak M, Mazur A, Król J., Microb Cell Fact 5(), 2006
PMID: 16483356
Effects of N-starvation and C-source on Bradyrhizobium japonicum exopolysaccharide production and composition, and bacterial infectivity to soybean roots.
Quelas JI, López-García SL, Casabuono A, Althabegoiti MJ, Mongiardini EJ, Pérez-Giménez J, Couto A, Lodeiro AR., Arch Microbiol 186(2), 2006
PMID: 16802172
Protein-tyrosine phosphorylation in Bacillus subtilis.
Mijakovic I, Petranovic D, Bottini N, Deutscher J, Ruhdal Jensen P., J Mol Microbiol Biotechnol 9(3-4), 2005
PMID: 16415592
How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF.
Mijakovic I, Petranovic D, Deutscher J., J Mol Microbiol Biotechnol 8(1), 2004
PMID: 15741737
The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases.
Moreira LM, Hoffmann K, Albano H, Becker A, Niehaus K, Sá-Correia I., J Mol Microbiol Biotechnol 8(1), 2004
PMID: 15741740
Autophosphorylation of the Escherichia coli protein kinase Wzc regulates tyrosine phosphorylation of Ugd, a UDP-glucose dehydrogenase.
Grangeasse C, Obadia B, Mijakovic I, Deutscher J, Cozzone AJ, Doublet P., J Biol Chem 278(41), 2003
PMID: 12851388
Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases.
Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Jamet E, Decottignies P, Grangeasse C, Doublet P, Le Maréchal P, Deutscher J., EMBO J 22(18), 2003
PMID: 12970183
Identification and physical organization of the gene cluster involved in the biosynthesis of Burkholderia cepacia complex exopolysaccharide.
Moreira LM, Videira PA, Sousa SA, Leitão JH, Cunha MV, Sá-Correia I., Biochem Biophys Res Commun 312(2), 2003
PMID: 14637140
Molecular basis of bacterial outer membrane permeability revisited.
Nikaido H., Microbiol Mol Biol Rev 67(4), 2003
PMID: 14665678
Rhizobium leguminosarum bv. trifolii PssP protein is required for exopolysaccharide biosynthesis and polymerization.
Mazur A, Król JE, Wielbo J, Urbanik-Sypniewska T, Skorupska A., Mol Plant Microbe Interact 15(4), 2002
PMID: 12026178
Staphylococcus aureus contains two low-molecular-mass phosphotyrosine protein phosphatases.
Soulat D, Vaganay E, Duclos B, Genestier AL, Etienne J, Cozzone AJ., J Bacteriol 184(18), 2002
PMID: 12193638

55 References

Daten bereitgestellt von Europe PubMed Central.

Structural elucidation using HPLC-MS of the acidic polysaccharide secreted by strain Rm1021
Aman P, McNeil M, Franzen L, Darvill A, Albersheim P., 1981
Specific oligosaccharide form of the Rhizobium meliloti exopolysaccharide promotes nodule invasion in alfalfa.
Battisti L, Lara JC, Leigh JA., Proc. Natl. Acad. Sci. U.S.A. 89(12), 1992
PMID: 1608972
R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098
Osmotically induced oligo- and polysaccharide synthesis by SU47
Breedveld M, Zevenhuisen L, Zehnder A., 1990
XL1-Blue: a high efficiency plasmid transforming strain with beta-galactoside selection
Bullock W, Fernandez J, Short J., 1987
Identification and characterization of large plasmids in using agarose gel electrophoresis
Casse F, Boucher C, Hulliot J, Michel M, Denarie J., 1979
Reducing sugar-neocuprine assay
Chaplin M., 1986
On the binding of ATP to the autophosphorylating protein, Ptk, of the bacterium Acinetobacter johnsonii.
Doublet P, Vincent C, Grangeasse C, Cozzone AJ, Duclos B., FEBS Lett. 445(1), 1999
PMID: 10069388
Signalling by receptor tyrosine kinases.
Fantl WJ, Johnson DE, Williams LT., Annu. Rev. Biochem. 62(), 1993
PMID: 7688944
Family of glycosyl transferases needed for the synthesis of succinoglycan by Rhizobium meliloti.
Glucksmann MA, Reuber TL, Walker GC., J. Bacteriol. 175(21), 1993
PMID: 8226645
Low molecular weight EPS II of Rhizobium meliloti allows nodule invasion in Medicago sativa.
Gonzalez JE, Reuhs BL, Walker GC., Proc. Natl. Acad. Sci. U.S.A. 93(16), 1996
PMID: 8710923
Characterization of a bacterial gene encoding an autophosphorylating protein tyrosine kinase.
Grangeasse C, Doublet P, Vaganay E, Vincent C, Deleage G, Duclos B, Cozzone AJ., Gene 204(1-2), 1997
PMID: 9434192
ABC transporters: from microorganisms to man.
Higgins CF., Annu. Rev. Cell Biol. 8(), 1992
PMID: 1282354
Protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysaccharide.
Ilan O, Bloch Y, Frankel G, Ullrich H, Geider K, Rosenshine I., EMBO J. 18(12), 1999
PMID: 10369665

Kessler C., 1992
Protein exporter function and in vitro ATPase activity are correlated in ABC-domain mutants of HlyB.
Koronakis E, Hughes C, Milisav I, Koronakis V., Mol. Microbiol. 16(1), 1995
PMID: 7651140
An improved assay for nanomole amounts of inorganic phosphate.
Lanzetta PA, Alvarez LJ, Reinach PS, Candia OA., Anal. Biochem. 100(1), 1979
PMID: 161695
Exopolysaccharides of Rhizobium: synthesis, regulation and symbiotic function.
Leigh JA, Walker GC., Trends Genet. 10(2), 1994
PMID: 8191588
Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules.
Leigh JA, Signer ER, Walker GC., Proc. Natl. Acad. Sci. U.S.A. 82(18), 1985
PMID: 3862129
Evaluation of Wzz/MPA1/MPA2 proteins based on the presence of coiled-coil regions.
Morona R, Van Den Bosch L, Daniels C., Microbiology (Reading, Engl.) 146 ( Pt 1)(), 2000
PMID: 10658645
Genetic analysis of the Rhizobium meliloti exoYFQ operon: ExoY is homologous to sugar transferases and ExoQ represents a transmembrane protein.
Muller P, Keller M, Weng WM, Quandt J, Arnold W, Puhler A., Mol. Plant Microbe Interact. 6(1), 1993
PMID: 8439670
The role of microbial surface polysaccharides in the -legume interaction
Niehaus K, Becker A., 1998
Computer-based analyses of the protein constituents of transport systems catalysing export of complex carbohydrates in bacteria.
Paulsen IT, Beness AM, Saier MH Jr., Microbiology (Reading, Engl.) 143 ( Pt 8)(), 1997
PMID: 9274022
Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021.
Reinhold BB, Chan SY, Reuber TL, Marra A, Walker GC, Reinhold VN., J. Bacteriol. 176(7), 1994
PMID: 8144468

Sambrook J, Fritsch E, Maniatis T., 1989
The P-loop--a common motif in ATP- and GTP-binding proteins.
Saraste M, Sibbald PR, Wittinghofer A., Trends Biochem. Sci. 15(11), 1990
PMID: 2126155
A broad host range mobilization system for in vitro genetic engineering: transposon mutagenesis in Gram-negative bacteria
Simon R, Priefer U, Pühler A., 1983
Lipid-bound sugars in Rhizobium meliloti.
Tolmasky ME, Staneloni RJ, Ugalde RA, Leloir LF., Arch. Biochem. Biophys. 203(1), 1980
PMID: 6447479
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Towbin H, Staehelin T, Gordon J., Proc. Natl. Acad. Sci. U.S.A. 76(9), 1979
PMID: 388439
Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in gram-negative bacteria.
Vincent C, Duclos B, Grangeasse C, Vaganay E, Riberty M, Cozzone AJ, Doublet P., J. Mol. Biol. 304(3), 2000
PMID: 11090276
Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb.
Vincent C, Doublet P, Grangeasse C, Vaganay E, Cozzone AJ, Duclos B., J. Bacteriol. 181(11), 1999
PMID: 10348860
Exopolysaccharides and their role in nodule invasion
York G, González J, Walker G., 1996
(1,2)-β--Glucan and acidic oligosaccharides produced by
Zevenhuisen L, van A., 1983
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