Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates

Disque-Kochem C, Battermann A, Stratz M, Dreiseikelmann B (2001)
MICROBIOLOGICAL RESEARCH 156(2): 159-168.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Disque-Kochem, C; Battermann, A; Stratz, M; Dreiseikelmann, BrigitteUniBi
Einrichtung
Fakultät für Biologie > Gentechnologie/Mikrobiologie
Abstract / Bemerkung
The transfer regions of different conjugative plasmids show significant similarities in the genetic organization and in the amino acid sequence of some gene products, especially of proteins from the traG or trbB family. These similarities are also evident on the level of the nucleotide sequences. On the basis of conserved DNA regions we designed degenerate PCR primer pairs to detect specifically tra regions within a collection of bacterial clones isolated from an agricultural soil. Most of the potential transfer-proficient indigenous bacterial isolates were able to mobilize a derivative of the nonconjugative IncQ plasmid RSF1010 into recipient strains. With the help of the primers it should be possible to evaluate the genetic potential for horizontal gene transfer carried out by conjugative plasmids.
Stichworte
conjugative plasmids-soil bacteria-degenerate; primers-TraG/TrbB family
Erscheinungsjahr
2001
Zeitschriftentitel
MICROBIOLOGICAL RESEARCH
Band
156
Ausgabe
2
Seite(n)
159-168
ISSN
0944-5013
Page URI
https://pub.uni-bielefeld.de/record/1616242

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Disque-Kochem C, Battermann A, Stratz M, Dreiseikelmann B. Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH. 2001;156(2):159-168.
Disque-Kochem, C., Battermann, A., Stratz, M., & Dreiseikelmann, B. (2001). Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH, 156(2), 159-168. https://doi.org/10.1078/0944-5013-00098
Disque-Kochem, C, Battermann, A, Stratz, M, and Dreiseikelmann, Brigitte. 2001. “Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates”. MICROBIOLOGICAL RESEARCH 156 (2): 159-168.
Disque-Kochem, C., Battermann, A., Stratz, M., and Dreiseikelmann, B. (2001). Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH 156, 159-168.
Disque-Kochem, C., et al., 2001. Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH, 156(2), p 159-168.
C. Disque-Kochem, et al., “Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates”, MICROBIOLOGICAL RESEARCH, vol. 156, 2001, pp. 159-168.
Disque-Kochem, C., Battermann, A., Stratz, M., Dreiseikelmann, B.: Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH. 156, 159-168 (2001).
Disque-Kochem, C, Battermann, A, Stratz, M, and Dreiseikelmann, Brigitte. “Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates”. MICROBIOLOGICAL RESEARCH 156.2 (2001): 159-168.

3 Zitationen in Europe PMC

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