A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis

Kirchner O, Gartemann K-H, Zellermann EM, Eichenlaub R, Burger A (2001)
MOLECULAR PLANT-MICROBE INTERACTIONS 14(11): 1312-1318.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Kirchner, O; Gartemann, Karl-HeinzUniBi ; Zellermann, EM; Eichenlaub, RudolfUniBi; Burger, A
Einrichtung
Centrum für Biotechnologie
Fakultät für Biologie > Gentechnologie/Mikrobiologie
Abstract / Bemerkung
A transposon mutagenesis system for Clavibacter michiganensis subsp. michiganensis was developed based on antibiotic resistance transposons that were derived from the insertion element IS1409 from Arthrobacter sp. strain TM1 NCIB12013. As a prerequisite, the electroporation efficiency was optimized by using unmethylated DNA and treatment of the cells with glycine such that about 5 x 10(6) transformants per jag of DNA were generally obtained. Electroporation of C. michiganensis subsp. michiganensis with a suicide vector carrying transposon Tn1409C resulted in approximately 1 x 10(3) transposon mutants per mug of DNA and thus is suitable for saturation mutagenesis. Analysis of Tn1409C insertion sites suggests a random mode of transposition. Transposition of Tn1409C was also demonstrated for other subspecies of C. michiganensis.
Erscheinungsjahr
2001
Zeitschriftentitel
MOLECULAR PLANT-MICROBE INTERACTIONS
Band
14
Ausgabe
11
Seite(n)
1312-1318
ISSN
0894-0282
Page URI
https://pub.uni-bielefeld.de/record/1615932

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Kirchner O, Gartemann K-H, Zellermann EM, Eichenlaub R, Burger A. A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS. 2001;14(11):1312-1318.
Kirchner, O., Gartemann, K. - H., Zellermann, E. M., Eichenlaub, R., & Burger, A. (2001). A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS, 14(11), 1312-1318. https://doi.org/10.1094/MPMI.2001.14.11.1312
Kirchner, O, Gartemann, Karl-Heinz, Zellermann, EM, Eichenlaub, Rudolf, and Burger, A. 2001. “A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis”. MOLECULAR PLANT-MICROBE INTERACTIONS 14 (11): 1312-1318.
Kirchner, O., Gartemann, K. - H., Zellermann, E. M., Eichenlaub, R., and Burger, A. (2001). A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS 14, 1312-1318.
Kirchner, O., et al., 2001. A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS, 14(11), p 1312-1318.
O. Kirchner, et al., “A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis”, MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 14, 2001, pp. 1312-1318.
Kirchner, O., Gartemann, K.-H., Zellermann, E.M., Eichenlaub, R., Burger, A.: A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS. 14, 1312-1318 (2001).
Kirchner, O, Gartemann, Karl-Heinz, Zellermann, EM, Eichenlaub, Rudolf, and Burger, A. “A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis”. MOLECULAR PLANT-MICROBE INTERACTIONS 14.11 (2001): 1312-1318.

18 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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