Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii

Strauss C, Mussgnug JH, Kruse O (2001)
Photosynthesis Research 70(3): 311-320.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
To improve the analysis of unknown flanking DNA sequences adjacent to known sequences in nuclear genomes of photoautotrophic eukaryotic organisms, we established the technique of ligation-mediated suppression-PCR (LMS-PCR) in the green alga Chlamydomonas reinhardtii for (1) walking from a specific nuclear insertion fragment of random knockout mutants into the unknown flanking DNA sequence to identify and analyse disrupted genomic DNA regions and for (2) walking from highly conserved DNA regions derived from known gene iso-forms into flanking DNA sequences to identify new members of protein families. The feasibility of LMS-PCR for these applications was successfully demonstrated in two different approaches. The first resulted in the identification of a genomic DNA fragment flanking a nuclear insertion vector in a random knockout mutant whose phenotype was characterised by its inability to perform functional LHC state transitions. The second approach targeted the cab gene family. An oligonucleotide of a cabII gene, derived from a highly conserved region, was used to identify potential cab gene regions in the nuclear genome of Chlamydomonas. LMS-PCR combined with 3' rapid amplification of cDNA ends (3' RACE) and a PCR-based screening of a cDNA library resulted in the identification of the new cabII gene lhcb4. Both results clearly indicate that LMS-PCR is a powerful tool for the identification of flanking DNA sequences in the nuclear genome of Chlamydomonas reinhardtii.
Erscheinungsjahr
Zeitschriftentitel
Photosynthesis Research
Band
70
Ausgabe
3
Seite(n)
311-320
ISSN
PUB-ID

Zitieren

Strauss C, Mussgnug JH, Kruse O. Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii. Photosynthesis Research. 2001;70(3):311-320.
Strauss, C., Mussgnug, J. H., & Kruse, O. (2001). Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii. Photosynthesis Research, 70(3), 311-320. doi:10.1023/A:1014713612509
Strauss, C., Mussgnug, J. H., and Kruse, O. (2001). Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii. Photosynthesis Research 70, 311-320.
Strauss, C., Mussgnug, J.H., & Kruse, O., 2001. Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii. Photosynthesis Research, 70(3), p 311-320.
C. Strauss, J.H. Mussgnug, and O. Kruse, “Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii”, Photosynthesis Research, vol. 70, 2001, pp. 311-320.
Strauss, C., Mussgnug, J.H., Kruse, O.: Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii. Photosynthesis Research. 70, 311-320 (2001).
Strauss, C., Mussgnug, Jan H., and Kruse, Olaf. “Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii”. Photosynthesis Research 70.3 (2001): 311-320.

8 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Raa4 is a trans-splicing factor that specifically binds chloroplast tscA intron RNA.
Glanz S, Jacobs J, Kock V, Mishra A, Kück U., Plant J 69(3), 2012
PMID: 21954961
Loop-linker PCR: an advanced PCR technique for genome walking.
Trinh Q, Shi H, Xu W, Hao J, Luo Y, Huang K., IUBMB Life 64(10), 2012
PMID: 23008115
The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii.
Schönfeld C, Wobbe L, Borgstädt R, Kienast A, Nixon PJ, Kruse O., J Biol Chem 279(48), 2004
PMID: 15448140
Proteomics of Chlamydomonas reinhardtii light-harvesting proteins.
Stauber EJ, Fink A, Markert C, Kruse O, Johanningmeier U, Hippler M., Eukaryot Cell 2(5), 2003
PMID: 14555480

22 References

Daten bereitgestellt von Europe PubMed Central.

Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.
Kindle KL, Schnell RA, Fernandez E, Lefebvre PA., J. Cell Biol. 109(6 Pt 1), 1989
PMID: 2592399
Playing tag with Chlamydomonas.
Gumpel NJ, Purton S., Trends Cell Biol. 4(8), 1994
PMID: 14768635
The genetics and cytology of Chlamydomonas.
LEVINE RP, EBERSOLD WT., Annu. Rev. Microbiol. 14(), 1960
PMID: 13761500
Transformation of the green alga Chlamydomonas reinhardii with yeast DNA.
Rochaix JD, van Dillewijn J., Nature 296(5852), 1982
PMID: 7038510
A guide to the Lhc genes and their relatives in Arabidopsis/IT>
Jansson S., Trends Plant Sci. 4(6), 1999
PMID: 10366881
Isolation and characterization of photoautotrophic mutants of Chlamydomonas reinhardtii deficient in state transition.
Fleischmann MM, Ravanel S, Delosme R, Olive J, Zito F, Wollman FA, Rochaix JD., J. Biol. Chem. 274(43), 1999
PMID: 10521495
The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.
Stevens DR, Rochaix JD, Purton S., Mol. Gen. Genet. 251(1), 1996
PMID: 8628243
Chlamydomonas reinhardtii as the photosynthetic yeast.
Rochaix JD., Annu. Rev. Genet. 29(), 1995
PMID: 8825474
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
Kindle KL., Proc. Natl. Acad. Sci. U.S.A. 87(3), 1990
PMID: 2105499
An improved PCR method for walking in uncloned genomic DNA.
Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA., Nucleic Acids Res. 23(6), 1995
PMID: 7731798
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
Arabidopsis Genome Initiative., Nature 408(6814), 2000
PMID: 11130711
Structure of the Chlamydomonas reinhardtii cabII-1 gene encoding a chlorophyll-a/b-binding protein.
Imbault P, Wittemer C, Johanningmeier U, Jacobs JD, Howell SH., Gene 73(2), 1988
PMID: 3072265
Green yeast.
Goodenough UW., Cell 70(4), 1992
PMID: 1505022
Chlamydomonas genetics, a tool for the study of bioenergetic pathways.
Hippler M, Redding K, Rochaix JD., Biochim. Biophys. Acta 1367(1-3), 1998
PMID: 9784589

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 16252176
PubMed | Europe PMC

Suchen in

Google Scholar