Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles
Kakorin S, Neumann E (2002)
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 209(2-3). ELSEVIER SCIENCE BV: 147-165.
Konferenzbeitrag
| Veröffentlicht | Englisch
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Abstract / Bemerkung
The classical interpretation of the experimental electric turbidity and absorbance dichroisms has been extended to account for chemical contributions to the dichroitic signal caused by changes in the immediate neighborhood of the scattering centers or chromophores. In diluted suspensions of lipid bilayer vesicles (Lecithin (20%), concentration I mM) of radius a = 90 nm, doped with diphenylhexatrienyl-phosphatidylcholine (beta-DPH pPC, 5 muM), the chemical modes refer to the entrance of water into the head-group region of the lipids in the bilayer and into those membrane parts constituting the electropores. We propose here, an extended analytical approach to correctly analyze turbidity and absorbance relaxation data on membrane electroporation (ME) and electro-elongation of lipid vesicles. It is shown that the combination of turbidity and absorbance data provides a tool to quantify molecular order and rapid motion of optical probes within the vesicle bilayer in terms of order parameters. (C) 2002 Elsevier Science B.V. All rights reserved.
Stichworte
lipid order;
turbidity;
vesicle electro-elongation;
membrane electroporation;
absorbance dichroism
Erscheinungsjahr
2002
Titel des Konferenzbandes
Colloids and Surfaces A: Physicochemical and Engineering Aspects
Serien- oder Zeitschriftentitel
COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Band
209
Ausgabe
2-3
Seite(n)
147-165
ISSN
0927-7757
Page URI
https://pub.uni-bielefeld.de/record/1613606
Zitieren
Kakorin S, Neumann E. Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles. In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS. Vol 209. ELSEVIER SCIENCE BV; 2002: 147-165.
Kakorin, S., & Neumann, E. (2002). Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 209, 147-165. ELSEVIER SCIENCE BV. https://doi.org/10.1016/S0927-7757(02)00176-0
Kakorin, Sergej, and Neumann, Eberhard. 2002. “Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles”. In Colloids and Surfaces A: Physicochemical and Engineering Aspects, 209:147-165. COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS. ELSEVIER SCIENCE BV.
Kakorin, S., and Neumann, E. (2002). “Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles” in Colloids and Surfaces A: Physicochemical and Engineering Aspects COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS, vol. 209, (ELSEVIER SCIENCE BV), 147-165.
Kakorin, S., & Neumann, E., 2002. Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles. In Colloids and Surfaces A: Physicochemical and Engineering Aspects. COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS. no.209 ELSEVIER SCIENCE BV, pp. 147-165.
S. Kakorin and E. Neumann, “Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles”, Colloids and Surfaces A: Physicochemical and Engineering Aspects, COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS, vol. 209, ELSEVIER SCIENCE BV, 2002, pp.147-165.
Kakorin, S., Neumann, E.: Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles. Colloids and Surfaces A: Physicochemical and Engineering Aspects. COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS. 209, p. 147-165. ELSEVIER SCIENCE BV (2002).
Kakorin, Sergej, and Neumann, Eberhard. “Electrooptical relaxation spectrometry of membrane electroporation in lipid vesicles”. Colloids and Surfaces A: Physicochemical and Engineering Aspects. ELSEVIER SCIENCE BV, 2002.Vol. 209. COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS. 147-165.
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