The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin

Walders-Harbeck B, Khaitlina SY, Hinssen H, Jockusch BM, Illenberger S (2002)
FEBS LETTERS 529(2-3): 275-280.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Walders-Harbeck, B; Khaitlina, SY; Hinssen, HorstUniBi; Jockusch, BM; Illenberger, S
Abstract / Bemerkung
The vasodilator-stimulated phosphoprotein (VASP) functions as a cellular regulator of actin dynamics. VASP may initialise actin polymerisation, suggesting a direct interaction with monomeric actin. The present study demonstrates that VASP directly binds to actin monomers and that complex formation depends on a conserved four amino acid motif in the EVH2 domain. Point mutations within this motif drastically weaken VASP/G-actin interactions, thereby abolishing any actin-nucleating activity of VASP. Additionally, actin nucleation was found to depend on VASP oligomerisation since VASP monomers fail to induce the formation of actin filaments. Phosphorylation negatively affects VASP/G-actin interactions preventing VASP-induced actin filament formation. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Ena/Mena/vasodilator-stimulated phosphoprotein; actin dynamics; G-actin; nucleation; polymerisation
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Walders-Harbeck B, Khaitlina SY, Hinssen H, Jockusch BM, Illenberger S. The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin. FEBS LETTERS. 2002;529(2-3):275-280.
Walders-Harbeck, B., Khaitlina, S. Y., Hinssen, H., Jockusch, B. M., & Illenberger, S. (2002). The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin. FEBS LETTERS, 529(2-3), 275-280.
Walders-Harbeck, B, Khaitlina, SY, Hinssen, Horst, Jockusch, BM, and Illenberger, S. 2002. “The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin”. FEBS LETTERS 529 (2-3): 275-280.
Walders-Harbeck, B., Khaitlina, S. Y., Hinssen, H., Jockusch, B. M., and Illenberger, S. (2002). The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin. FEBS LETTERS 529, 275-280.
Walders-Harbeck, B., et al., 2002. The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin. FEBS LETTERS, 529(2-3), p 275-280.
B. Walders-Harbeck, et al., “The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin”, FEBS LETTERS, vol. 529, 2002, pp. 275-280.
Walders-Harbeck, B., Khaitlina, S.Y., Hinssen, H., Jockusch, B.M., Illenberger, S.: The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin. FEBS LETTERS. 529, 275-280 (2002).
Walders-Harbeck, B, Khaitlina, SY, Hinssen, Horst, Jockusch, BM, and Illenberger, S. “The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin”. FEBS LETTERS 529.2-3 (2002): 275-280.

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