Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor

Jockusch H, Voigt S, Eberhard D (2003)
Journal of Histochemistry & Cytochemistry 51(3): 401-404.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Green fluorescent protein (GFP) and its variants, such as enhanced GFP (EGFP), have been introduced into mammalian cells by transgenes, e.g., to distinguish donor from host cells after transplantation. Free GFP is extremely soluble and leaks out from liquid-covered cryostat sections so that fixation of whole organs before sectioning has been mandatory. This precludes the analysis of serial sections with respect to fixation-sensitive enzyme activities and antigens. We describe here a vapor fixation for sections from unfixed cryostat blocks of tissue that allows unrestricted enzyme and immunohistochemistry on adjacent sections, as demonstrated for cross-striated muscle and other tissues from EGFP transgenic "green mice" and for a transplantation experiment.
Stichworte
vapor fixation; transgenes; green fluorescent protein
Erscheinungsjahr
2003
Zeitschriftentitel
Journal of Histochemistry & Cytochemistry
Band
51
Ausgabe
3
Seite(n)
401-404
ISSN
0022-1554
eISSN
1551-5044
Page URI
https://pub.uni-bielefeld.de/record/1612496

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Jockusch H, Voigt S, Eberhard D. Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry. 2003;51(3):401-404.
Jockusch, H., Voigt, S., & Eberhard, D. (2003). Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry, 51(3), 401-404. doi:10.1177/002215540305100315
Jockusch, H., Voigt, S., and Eberhard, D. (2003). Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry 51, 401-404.
Jockusch, H., Voigt, S., & Eberhard, D., 2003. Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry, 51(3), p 401-404.
H. Jockusch, S. Voigt, and D. Eberhard, “Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor”, Journal of Histochemistry & Cytochemistry, vol. 51, 2003, pp. 401-404.
Jockusch, H., Voigt, S., Eberhard, D.: Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry. 51, 401-404 (2003).
Jockusch, Harald, Voigt, Sylvana, and Eberhard, Daniel. “Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor”. Journal of Histochemistry & Cytochemistry 51.3 (2003): 401-404.

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PMID: 12588968
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