Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide

Sharypova LA, Niehaus K, Scheidle H, Holst O, Becker A (2003)
JOURNAL OF BIOLOGICAL CHEMISTRY 278(15): 12946-12954.

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Autor*in
Sharypova, L. A.; Niehaus, KarstenUniBi; Scheidle, H.; Holst, O.; Becker, A.
Abstract / Bemerkung
Lipid A is the hydrophobic anchor of lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria. Lipid A of all Rhizobiaceae is acylated with a long fatty acid chain, 27-hydroxyoctacosanoic acid. Biosynthesis of this long acyl substitution requires a special acyl carrier protein, AcpXL, which serves as a donor of C28 (omega-1)-hydroxylated fatty acid for acylation of rhizobial lipid A (Brozek, K.A., Carlson, R.W., and Raetz, C. R. (1996) J. BioL Chem 271,32126-32136). To determine the biological function of the C28 acylation of lipid A, we constructed an acpXL mutant of Sinorhizobium meliloti strain 1021. Gas-liquid chromatography and mass spectrometry analysis of the fatty acid composition showed that the acpXL mutation indeed blocked C28 acylation of lipid A. SDS-PAGE analysis of acpXL mutant LPS revealed only a fast migrating band, rough LPS, whereas the parental strain 1021 manifested both rough and smooth LPS. Regardless of this, the LPS of parental and mutant strains had a similar sugar composition and exposed the same antigenic epitopes, implying that different electrophoretic profiles might account for different aggregation properties of LPS molecules with and without a long acyl chain. The acpXL mutant of strain 1021 displayed sensitivity to deoxycholate, delayed nodulation of Medicago sativa, and a reduced competitive ability. However, nodules elicited by this mutant on roots of M. sativa and Medicago truncatula had a normal morphology and fixed nitrogen. Thus, the C28 fatty acid moiety of lipid A is not crucial, but it is beneficial for establishing an effective symbiosis with host plants. acpXL lies upstream from a cluster of five genes, including msbB (lpxXL), which might be also involved in biosynthesis and transfer of the C28 fatty acid to the lipid A precursor.
Erscheinungsjahr
2003
Zeitschriftentitel
JOURNAL OF BIOLOGICAL CHEMISTRY
Band
278
Ausgabe
15
Seite(n)
12946-12954
ISSN
0021-9258
eISSN
1083-351X
Page URI
https://pub.uni-bielefeld.de/record/1612098

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Sharypova LA, Niehaus K, Scheidle H, Holst O, Becker A. Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide. JOURNAL OF BIOLOGICAL CHEMISTRY. 2003;278(15):12946-12954.
Sharypova, L. A., Niehaus, K., Scheidle, H., Holst, O., & Becker, A. (2003). Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide. JOURNAL OF BIOLOGICAL CHEMISTRY, 278(15), 12946-12954. https://doi.org/10.1074/jbc.M209389200
Sharypova, L. A., Niehaus, Karsten, Scheidle, H., Holst, O., and Becker, A. 2003. “Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide”. JOURNAL OF BIOLOGICAL CHEMISTRY 278 (15): 12946-12954.
Sharypova, L. A., Niehaus, K., Scheidle, H., Holst, O., and Becker, A. (2003). Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide. JOURNAL OF BIOLOGICAL CHEMISTRY 278, 12946-12954.
Sharypova, L.A., et al., 2003. Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide. JOURNAL OF BIOLOGICAL CHEMISTRY, 278(15), p 12946-12954.
L.A. Sharypova, et al., “Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, 2003, pp. 12946-12954.
Sharypova, L.A., Niehaus, K., Scheidle, H., Holst, O., Becker, A.: Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide. JOURNAL OF BIOLOGICAL CHEMISTRY. 278, 12946-12954 (2003).
Sharypova, L. A., Niehaus, Karsten, Scheidle, H., Holst, O., and Becker, A. “Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide”. JOURNAL OF BIOLOGICAL CHEMISTRY 278.15 (2003): 12946-12954.

36 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Cell Autoaggregation, Biofilm Formation, and Plant Attachment in a Sinorhizobium meliloti lpsB Mutant.
Sorroche F, Bogino P, Russo DM, Zorreguieta A, Nievas F, Morales GM, Hirsch AM, Giordano W., Mol Plant Microbe Interact 31(10), 2018
PMID: 30136892
Genome sequencing of two Neorhizobium galegae strains reveals a noeT gene responsible for the unusual acetylation of the nodulation factors.
Österman J, Marsh J, Laine PK, Zeng Z, Alatalo E, Sullivan JT, Young JP, Thomas-Oates J, Paulin L, Lindström K., BMC Genomics 15(), 2014
PMID: 24948393
Molecular insights into bacteroid development during Rhizobium-legume symbiosis.
Haag AF, Arnold MF, Myka KK, Kerscher B, Dall'Angelo S, Zanda M, Mergaert P, Ferguson GP., FEMS Microbiol Rev 37(3), 2013
PMID: 22998605
The DivJ, CbrA and PleC system controls DivK phosphorylation and symbiosis in Sinorhizobium meliloti.
Pini F, Frage B, Ferri L, De Nisco NJ, Mohapatra SS, Taddei L, Fioravanti A, Dewitte F, Galardini M, Brilli M, Villeret V, Bazzicalupo M, Mengoni A, Walker GC, Becker A, Biondi EG., Mol Microbiol 90(1), 2013
PMID: 23909720
Structure of a specialized acyl carrier protein essential for lipid A biosynthesis with very long-chain fatty acids in open and closed conformations.
Ramelot TA, Rossi P, Forouhar F, Lee HW, Yang Y, Ni S, Unser S, Lew S, Seetharaman J, Xiao R, Acton TB, Everett JK, Prestegard JH, Hunt JF, Montelione GT, Kennedy MA., Biochemistry 51(37), 2012
PMID: 22876860
Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.).
Ormeño-Orrillo E, Menna P, Almeida LG, Ollero FJ, Nicolás MF, Pains Rodrigues E, Shigueyoshi Nakatani A, Silva Batista JS, Oliveira Chueire LM, Souza RC, Ribeiro Vasconcelos AT, Megías M, Hungria M, Martínez-Romero E., BMC Genomics 13(), 2012
PMID: 23270491
Nitric oxide production induced in roots of Lotus japonicus by lipopolysaccharide from Mesorhizobium loti.
Murakami E, Nagata M, Shimoda Y, Kucho K, Higashi S, Abe M, Hashimoto M, Uchiumi T., Plant Cell Physiol 52(4), 2011
PMID: 21330297
Role of BacA in lipopolysaccharide synthesis, peptide transport, and nodulation by Rhizobium sp. strain NGR234.
Ardissone S, Kobayashi H, Kambara K, Rummel C, Noel KD, Walker GC, Broughton WJ, Deakin WJ., J Bacteriol 193(9), 2011
PMID: 21357487
Biochemical characterization of Sinorhizobium meliloti mutants reveals gene products involved in the biosynthesis of the unusual lipid A very long-chain fatty acid.
Haag AF, Wehmeier S, Muszyński A, Kerscher B, Fletcher V, Berry SH, Hold GL, Carlson RW, Ferguson GP., J Biol Chem 286(20), 2011
PMID: 21454518
FadD is required for utilization of endogenous fatty acids released from membrane lipids.
Pech-Canul Á, Nogales J, Miranda-Molina A, Álvarez L, Geiger O, Soto MJ, López-Lara IM., J Bacteriol 193(22), 2011
PMID: 21926226
SMc01553 is the sixth acyl carrier protein in Sinorhizobium meliloti 1021.
Dávila-Martínez Y, Ramos-Vega AL, Contreras-Martínez S, Encarnación S, Geiger O, López-Lara IM., Microbiology 156(pt 1), 2010
PMID: 19797355
Altered lipid A structures and polymyxin hypersensitivity of Rhizobium etli mutants lacking the LpxE and LpxF phosphatases.
Ingram BO, Sohlenkamp C, Geiger O, Raetz CR., Biochim Biophys Acta 1801(5), 2010
PMID: 20153447
Internalization of a thiazole-modified peptide in Sinorhizobium meliloti occurs by BacA-dependent and -independent mechanisms.
Wehmeier S, Arnold MF, Marlow VL, Aouida M, Myka KK, Fletcher V, Benincasa M, Scocchi M, Ramotar D, Ferguson GP., Microbiology 156(pt 9), 2010
PMID: 20507886
Importance of Lipopolysaccharide and Cyclic β-1,2-Glucans in Brucella-Mammalian Infections.
Haag AF, Myka KK, Arnold MF, Caro-Hernández P, Ferguson GP., Int J Microbiol 2010(), 2010
PMID: 21151694
Essential role for the BacA protein in the uptake of a truncated eukaryotic peptide in Sinorhizobium meliloti.
Marlow VL, Haag AF, Kobayashi H, Fletcher V, Scocchi M, Walker GC, Ferguson GP., J Bacteriol 191(5), 2009
PMID: 19074376
Symbiotic use of pathogenic strategies: rhizobial protein secretion systems.
Deakin WJ, Broughton WJ., Nat Rev Microbiol 7(4), 2009
PMID: 19270720
The Sinorhizobium meliloti LpxXL and AcpXL proteins play important roles in bacteroid development within alfalfa.
Haag AF, Wehmeier S, Beck S, Marlow VL, Fletcher V, James EK, Ferguson GP., J Bacteriol 191(14), 2009
PMID: 19429615
Rhizobium leguminosarum biovar viciae 3841, deficient in 27-hydroxyoctacosanoate-modified lipopolysaccharide, is impaired in desiccation tolerance, biofilm formation and motility.
Vanderlinde EM, Muszynski A, Harrison JJ, Koval SF, Foreman DL, Ceri H, Kannenberg EL, Carlson RW, Yost CK., Microbiology 155(pt 9), 2009
PMID: 19460825
Molecular determinants of a symbiotic chronic infection.
Gibson KE, Kobayashi H, Walker GC., Annu Rev Genet 42(), 2008
PMID: 18983260
Lipopolysaccharide structures from Agrobacterium and Rhizobiaceae species.
De Castro C, Molinaro A, Lanzetta R, Silipo A, Parrilli M., Carbohydr Res 343(12), 2008
PMID: 18353297
The Sinorhizobium meliloti MsbA2 protein is essential for the legume symbiosis.
Beck S, Marlow VL, Woodall K, Doerrler WT, James EK, Ferguson GP., Microbiology 154(pt 4), 2008
PMID: 18375818
The pea nodule environment restores the ability of a Rhizobium leguminosarum lipopolysaccharide acpXL mutant to add 27-hydroxyoctacosanoic acid to its lipid A.
Vedam V, Kannenberg E, Datta A, Brown D, Haynes-Gann JG, Sherrier DJ, Carlson RW., J Bacteriol 188(6), 2006
PMID: 16513742
CbrA is a stationary-phase regulator of cell surface physiology and legume symbiosis in Sinorhizobium meliloti.
Gibson KE, Campbell GR, Lloret J, Walker GC., J Bacteriol 188(12), 2006
PMID: 16740957
Importance of unusually modified lipid A in Sinorhizobium stress resistance and legume symbiosis.
Ferguson GP, Datta A, Carlson RW, Walker GC., Mol Microbiol 56(1), 2005
PMID: 15773979
Structural characterization of a flavonoid-inducible Pseudomonas aeruginosa A-band-like O antigen of Rhizobium sp. strain NGR234, required for the formation of nitrogen-fixing nodules.
Reuhs BL, Relić B, Forsberg LS, Marie C, Ojanen-Reuhs T, Stephens SB, Wong CH, Jabbouri S, Broughton WJ., J Bacteriol 187(18), 2005
PMID: 16159781
sinI- and expR-dependent quorum sensing in Sinorhizobium meliloti.
Gao M, Chen H, Eberhard A, Gronquist MR, Robinson JB, Rolfe BG, Bauer WD., J Bacteriol 187(23), 2005
PMID: 16291666
A Rhizobium leguminosarum lipopolysaccharide lipid-A mutant induces nitrogen-fixing nodules with delayed and defective bacteroid formation.
Vedam V, Haynes JG, Kannenberg EL, Carlson RW, Sherrier DJ., Mol Plant Microbe Interact 17(3), 2004
PMID: 15000395
Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid-A fatty acids.
Ferguson GP, Datta A, Baumgartner J, Roop RM, Carlson RW, Walker GC., Proc Natl Acad Sci U S A 101(14), 2004
PMID: 15044696
Molecular basis of bacterial outer membrane permeability revisited.
Nikaido H., Microbiol Mol Biol Rev 67(4), 2003
PMID: 14665678

56 References

Daten bereitgestellt von Europe PubMed Central.

Development of the legume root nodule.
Brewin NJ., Annu. Rev. Cell Biol. 7(), 1991
PMID: 1809347

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Occurrence of lipid A variants with 27-hydroxyoctacosanoic acid in lipopolysaccharides from members of the family Rhizobiaceae.
Bhat UR, Mayer H, Yokota A, Hollingsworth RI, Carlson RW., J. Bacteriol. 173(7), 1991
PMID: 2007543

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Characterization of the lipopolysaccharides from Rhizobium meliloti strain 102F51 and its nonnodulating mutant WL113.
Russa R, Bruneteau M, Shashkov AS, Urbanik-Sypniewska T, Mayer H., Arch. Microbiol. 165(1), 1996
PMID: 8639022
Sinorhizobium fredii and Sinorhizobium meliloti produce structurally conserved lipopolysaccharides and strain-specific K antigens.
Reuhs BL, Geller DP, Kim JS, Fox JE, Kolli VS, Pueppke SG., Appl. Environ. Microbiol. 64(12), 1998
PMID: 9835585
Chronic intracellular infection of alfalfa nodules by Sinorhizobium meliloti requires correct lipopolysaccharide core.
Campbell GR, Reuhs BL, Walker GC., Proc. Natl. Acad. Sci. U.S.A. 99(6), 2002
PMID: 11904442
Biochemistry of endotoxins.
Raetz CR., Annu. Rev. Biochem. 59(), 1990
PMID: 1695830

AUTHOR UNKNOWN, 0
Rhizobium lipopolysaccharide modulates infection thread development in white clover root hairs.
Dazzo FB, Truchet GL, Hollingsworth RI, Hrabak EM, Pankratz HS, Philip-Hollingsworth S, Salzwedel JL, Chapman K, Appenzeller L, Squartini A., J. Bacteriol. 173(17), 1991
PMID: 1885517

AUTHOR UNKNOWN, 0
The composite genome of the legume symbiont Sinorhizobium meliloti.
Galibert F, Finan TM, Long SR, Puhler A, Abola P, Ampe F, Barloy-Hubler F, Barnett MJ, Becker A, Boistard P, Bothe G, Boutry M, Bowser L, Buhrmester J, Cadieu E, Capela D, Chain P, Cowie A, Davis RW, Dreano S, Federspiel NA, Fisher RF, Gloux S, Godrie T, Goffeau A, Golding B, Gouzy J, Gurjal M, Hernandez-Lucas I, Hong A, Huizar L, Hyman RW, Jones T, Kahn D, Kahn ML, Kalman S, Keating DH, Kiss E, Komp C, Lelaure V, Masuy D, Palm C, Peck MC, Pohl TM, Portetelle D, Purnelle B, Ramsperger U, Surzycki R, Thebault P, Vandenbol M, Vorholter FJ, Weidner S, Wells DH, Wong K, Yeh KC, Batut J., Science 293(5530), 2001
PMID: 11474104
Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021.
Capela D, Barloy-Hubler F, Gouzy J, Bothe G, Ampe F, Batut J, Boistard P, Becker A, Boutry M, Cadieu E, Dreano S, Gloux S, Godrie T, Goffeau A, Kahn D, Kiss E, Lelaure V, Masuy D, Pohl T, Portetelle D, Puhler A, Purnelle B, Ramsperger U, Renard C, Thebault P, Vandenbol M, Weidner S, Galibert F., Proc. Natl. Acad. Sci. U.S.A. 98(17), 2001
PMID: 11481430

Casse, J. Bacteriol. 113(), 1979
A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa.
Lagares A, Caetano-Anolles G, Niehaus K, Lorenzen J, Ljunggren HD, Puhler A, Favelukes G., J. Bacteriol. 174(18), 1992
PMID: 1325969

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098

Westphal, Methods Carbohydr. Chem. 5(), 1965

AUTHOR UNKNOWN, 0
Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis.
Valverde C, Hozbor DF, Lagares A., BioTechniques 22(2), 1997
PMID: 9043688
The presence of a novel type of surface polysaccharide in Rhizobium meliloti requires a new fatty acid synthase-like gene cluster involved in symbiotic nodule development.
Petrovics G, Putnoky P, Reuhs B, Kim J, Thorp TA, Noel KD, Carlson RW, Kondorosi A., Mol. Microbiol. 8(6), 1993
PMID: 8361353

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Epitope identification for a panel of anti-Sinorhizobium meliloti monoclonal antibodies and application to the analysis of K antigens and lipopolysaccharides from bacteroids.
Reuhs BL, Stephens SB, Geller DP, Kim JS, Glenn J, Przytycki J, Ojanen-Reuhs T., Appl. Environ. Microbiol. 65(11), 1999
PMID: 10543844
Genetic characterization of a Sinorhizobium meliloti chromosomal region in lipopolysaccharide biosynthesis.
Lagares A, Hozbor DF, Niehaus K, Otero AJ, Lorenzen J, Arnold W, Puhler A., J. Bacteriol. 183(4), 2001
PMID: 11157937
Studies on the meningococcal polysaccharides. I. Composition and chemical properties of the group A polysaccharide.
Liu TY, Gotschlich EC, Jonssen EK, Wysocki JR., J. Biol. Chem. 246(9), 1971
PMID: 4995120
The lipooligosaccharides of pathogenic gram-negative bacteria.
Preston A, Mandrell RE, Gibson BW, Apicella MA., Crit. Rev. Microbiol. 22(3), 1996
PMID: 8894399
A lethal role for lipid A in Salmonella infections.
Khan SA, Everest P, Servos S, Foxwell N, Zahringer U, Brade H, Rietschel ET, Dougan G, Charles IG, Maskell DJ., Mol. Microbiol. 29(2), 1998
PMID: 9720873
A novel Escherichia coli lipid A mutant that produces an antiinflammatory lipopolysaccharide.
Somerville JE Jr, Cassiano L, Bainbridge B, Cunningham MD, Darveau RP., J. Clin. Invest. 97(2), 1996
PMID: 8567955
Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213.
Russa R, Urbanik-Sypniewska T, Lindstrom K, Mayer H., Arch. Microbiol. 163(5), 1995
PMID: 7794102
Occurrence and taxonomic significance of oxo-fatty acids in lipopolysaccharides from members of Mesorhizobium.
Choma A, Urbanik-Sypniewska T, Russa R, Kutkowska J, Mayer H., Syst. Appl. Microbiol. 23(2), 2000
PMID: 10930069
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