An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics

Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B (2003)
Plant Molecular Biology 53(1/2): 247-259.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ;
Abstract / Bemerkung
The GABI-Kat population of T-DNA mutagenized Arabidopsis thaliana lines with sequence-characterized insertion sites is used extensively for efficient progress in plant functional genomics. Here we provide details about the establishment of the material, demonstrate the population's functionality and discuss results from quality control studies. T-DNA insertion mutants of the accession Columbia (Col-0) were created by Agrobacterium tumefaciens-mediated transformation. To allow selection of transformed plants under greenhouse conditions, a sulfadiazine resistance marker was employed. DNA from leaves of T1 plants was extracted and used as a template for PCR-based amplification of DNA fragments spanning insertion site borders. After sequencing, the data were placed in a flanking sequence tag (FST) database describing which mutant allele was present in which line. Analysis of the distribution of T-DNA insertions revealed a clear bias towards intergenic regions. Insertion sites appeared more frequent in regions in front of the ATG and after STOP codons of predicted genes. Segregation analysis for sulfadiazine resistance showed that 62% of the transformants contain an insertion at only one genetic locus. In quality control studies with gene-specific primers in combination with T-DNA primers, 76% of insertions could be confirmed. Finally, the functionality of the GABI-Kat population was demonstrated by exemplary confirmation of several new transparent testa alleles, as well as a number of other mutants, which were identified on the basis of the FST data.
Stichworte
knock-out; insertion mutagenesis; gene function; FST; reverse genetics
Erscheinungsjahr
2003
Zeitschriftentitel
Plant Molecular Biology
Band
53
Ausgabe
1/2
Seite(n)
247-259
ISSN
0167-4412
Page URI
https://pub.uni-bielefeld.de/record/1609190

Zitieren

Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B. An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. Plant Molecular Biology. 2003;53(1/2):247-259.
Rosso, M. G., Li, Y., Strizhov, N., Reiss, B., Dekker, K., & Weisshaar, B. (2003). An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. Plant Molecular Biology, 53(1/2), 247-259. doi:10.1023/B:PLAN.0000009297.37235.4a
Rosso, M. G., Li, Y., Strizhov, N., Reiss, B., Dekker, K., and Weisshaar, B. (2003). An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. Plant Molecular Biology 53, 247-259.
Rosso, M.G., et al., 2003. An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. Plant Molecular Biology, 53(1/2), p 247-259.
M.G. Rosso, et al., “An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics”, Plant Molecular Biology, vol. 53, 2003, pp. 247-259.
Rosso, M.G., Li, Y., Strizhov, N., Reiss, B., Dekker, K., Weisshaar, B.: An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. Plant Molecular Biology. 53, 247-259 (2003).
Rosso, Mario G., Li, Yong, Strizhov, Nicolai, Reiss, Bernd, Dekker, Koen, and Weisshaar, Bernd. “An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics”. Plant Molecular Biology 53.1/2 (2003): 247-259.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 14756321
PubMed | Europe PMC

Suchen in

Google Scholar