article
Data analysis procedures for pulse ELDOR measurements of broad distance distributions
published
yes
G
Jeschke
author
G
Panek
author
Adelheid
Godt
author 101324
A
Bender
author
H
Paulsen
author
17976
department
The reliability of procedures for extracting the distance distribution between spins from the dipolar evolution function is studied with particular emphasis on broad distributions. A new numerically stable procedure for fitting distance distributions with polynomial interpolation between sampling points is introduced and compared to Tikhonov regularization in the dipolar frequency and distance domains and to approximate Pake transformation. Distance distributions with only narrow peaks are most reliably extracted by distance-domain Tikhonov regularization, while frequency-domain Tikhonov regularization is favorable for distributions with only broad peaks. For the quantification of distributions by their mean distance and variance, Hermite polynomial interpolation provides the best results. Distributions that contain both broad and narrow peaks are most difficult to analyze. In this case a high signal-to-noise ratio is strictly required and approximate Pake transformation should be applied. A procedure is given for renormalizing primary experimental data from protein preparations with slightly different degrees of spin labelling, so that they can be compared directly. Performance of all the data analysis procedures is demonstrated on experimental data for a shape-persistent biradical with a label-to-label distance of 5 nm, for a [2]catenane with a broad distance distribution, and for a doubly spin-labelled double mutant of plant light harvesting complex II.
SPRINGER WIEN2004
eng
APPLIED MAGNETIC RESONANCE
0937-9347
1613-7507
00022236600001710.1007/BF03166574
261-2223-244
Jeschke G, Panek G, Godt A, Bender A, Paulsen H (2004) <br /><em>APPLIED MAGNETIC RESONANCE</em> 26(1-2): 223-244.
Jeschke, G., Panek, G., Godt, A., Bender, A., & Paulsen, H. (2004). Data analysis procedures for pulse ELDOR measurements of broad distance distributions. <em>APPLIED MAGNETIC RESONANCE</em>, <em>26</em>(1-2), 223-244. doi:10.1007/BF03166574
<div style="text-indent:-25px; padding-left:25px;padding-bottom:0px;">Jeschke, G., Panek, G., Godt, A., Bender, A., & Paulsen, H. (2004). Data analysis procedures for pulse ELDOR measurements of broad distance distributions. <em>APPLIED MAGNETIC RESONANCE</em>, <em>26</em>(1-2), 223-244. doi:10.1007/BF03166574</div>
Jeschke, G., Panek, G., Godt, A., Bender, A., and Paulsen, H. (2004). Data analysis procedures for pulse ELDOR measurements of broad distance distributions. <em>APPLIED MAGNETIC RESONANCE</em> 26, 223-244.
Jeschke, G, Panek, G, Godt, Adelheid, Bender, A, and Paulsen, H. “Data analysis procedures for pulse ELDOR measurements of broad distance distributions”. <em>APPLIED MAGNETIC RESONANCE</em> 26.1-2 (2004): 223-244.
<div style="text-indent:-25px; padding-left:25px;padding-bottom:0px;">Jeschke, G, Panek, G, Godt, Adelheid, Bender, A, and Paulsen, H. 2004. “Data analysis procedures for pulse ELDOR measurements of broad distance distributions”. <em>APPLIED MAGNETIC RESONANCE</em> 26 (1-2): 223-244.</div>
G. Jeschke, et al., Data analysis procedures for pulse ELDOR measurements of broad distance distributions, APPLIED MAGNETIC RESONANCE <strong>26</strong>, 223 (2004).
Jeschke G, Panek G, Godt A, Bender A, Paulsen H. Data analysis procedures for pulse ELDOR measurements of broad distance distributions. <em>APPLIED MAGNETIC RESONANCE</em>. 2004;26(1-2):223-244.
G. Jeschke, G. Panek, A. Godt, A. Bender, and H. Paulsen, “Data analysis procedures for pulse ELDOR measurements of broad distance distributions”, <em>APPLIED MAGNETIC RESONANCE</em>, <strong>2004</strong>, <em>26</em>, 223-244.
Jeschke, G., Panek, G., Godt, A., Bender, A., Paulsen, H.: Data analysis procedures for pulse ELDOR measurements of broad distance distributions. APPLIED MAGNETIC RESONANCE. 26, 223-244 (2004).
<div style="text-indent:-25px; padding-left:25px;padding-bottom:0px;">Jeschke, G., Panek, G., Godt, A., Bender, A. & Paulsen, H. (2004). Data analysis procedures for pulse ELDOR measurements of broad distance distributions. <em>APPLIED MAGNETIC RESONANCE</em>, <em>26</em>(1-2), 223-244. SPRINGER WIEN. doi:10.1007/BF03166574.</div>
Jeschke, G.; Panek, G.; Godt, A.; Bender, A.; Paulsen, H. (2004): Data analysis procedures for pulse ELDOR measurements of broad distance distributions <em>APPLIED MAGNETIC RESONANCE</em>,26:(1-2): 223-244.
Jeschke, G., et al., 2004. Data analysis procedures for pulse ELDOR measurements of broad distance distributions. <em>APPLIED MAGNETIC RESONANCE</em>, 26(1-2), p 223-244.
Jeschke G, Panek G, Godt A, Bender A, Paulsen H (2004) <br />Data analysis procedures for pulse ELDOR measurements of broad distance distributions.<br />APPLIED MAGNETIC RESONANCE 26(1-2): 223-244.
G. Jeschke, et al., “Data analysis procedures for pulse ELDOR measurements of broad distance distributions”, <em>APPLIED MAGNETIC RESONANCE</em>, vol. 26, 2004, pp. 223-244.
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