The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP

Brecht M, Sewald K, Schiene K, Keen G, Fricke M, Sauer M, Niehaus K (2004)
Journal of Biotechnology 112(1-2): 151-164.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Brecht, M.; Sewald, K.; Schiene, K.; Keen, G.; Fricke, M.; Sauer, MarkusUniBi; Niehaus, KarstenUniBi
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe K. Niehaus
Fakultät für Biologie > Proteom- und Metabolomforschung
Fakultät für Physik
Abstract / Bemerkung
Using an RT-PCR approach a cDNA clone, designated Ms-Rac4 and putatively coding for a small GTPase was isolated from Medicago sativa. Ms-Rac4 and the earlier described Ms-Rac1 [Mol Gen. Genet. 263 (2000) 761] belong to the class of GTP-binding Rho of plants (Rop) proteins. At the amino acid level they display all conserved regions that are common to GTP-binding proteins. Phylogenetically both are located in the group la, but within this group they are well-separated. Computed structure models of both proteins revealed a high degree of structural conservation. Particularly the switch I and switch II region are 100% conserved between Ms-Rac1 and Ms-Rac4 and highly conserved as compared to other Rac-like G-proteins. Both GTPases differ in structure within the fourth loop and the fourth helix. GTP-binding properties of the heterologously expressed Ms-Rac1 and Ms-Rac4 was shown by fluorescence resonance energy transfer (FRET) using mantGTP and by surface plasmon resonance (SPR). By this method the specificity of the G-protein/GTP interaction was shown and the inhibitory effect of GTP, EDTA and Mg2+ on the Ms-Rac1 and Ms-Rac4 binding to immobilized GTP was characterized. Ms-Rac1 and Ms-Rac4 exhibited the same affinity to GTP and are similarly affected by GTP, EDTA and Mg2+. Thus, the predicted structural differences do not result in different GTP-binding properties of Ms-Rac1 and Ms-Rac4. (C) 2004 Elsevier B.V. All rights reserved.
Stichworte
Ms-Rac4; Ms-Rac1; medicago sativa; fluorescence; surface plasmon resonance (SPR); GTPases; resonance energy transfer (FRET); GTP-binding; biacore
Erscheinungsjahr
2004
Zeitschriftentitel
Journal of Biotechnology
Band
112
Ausgabe
1-2
Seite(n)
151-164
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1606737

Zitieren

Brecht M, Sewald K, Schiene K, et al. The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP. Journal of Biotechnology. 2004;112(1-2):151-164.
Brecht, M., Sewald, K., Schiene, K., Keen, G., Fricke, M., Sauer, M., & Niehaus, K. (2004). The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP. Journal of Biotechnology, 112(1-2), 151-164. https://doi.org/10.1016/j.jbiotec.2004.04.030
Brecht, M., Sewald, K., Schiene, K., Keen, G., Fricke, M., Sauer, Markus, and Niehaus, Karsten. 2004. “The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP”. Journal of Biotechnology 112 (1-2): 151-164.
Brecht, M., Sewald, K., Schiene, K., Keen, G., Fricke, M., Sauer, M., and Niehaus, K. (2004). The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP. Journal of Biotechnology 112, 151-164.
Brecht, M., et al., 2004. The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP. Journal of Biotechnology, 112(1-2), p 151-164.
M. Brecht, et al., “The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP”, Journal of Biotechnology, vol. 112, 2004, pp. 151-164.
Brecht, M., Sewald, K., Schiene, K., Keen, G., Fricke, M., Sauer, M., Niehaus, K.: The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP. Journal of Biotechnology. 112, 151-164 (2004).
Brecht, M., Sewald, K., Schiene, K., Keen, G., Fricke, M., Sauer, Markus, and Niehaus, Karsten. “The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP”. Journal of Biotechnology 112.1-2 (2004): 151-164.

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