The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii

Schonfeld C, Wobbe L, Borgstadt R, Kienast A, Nixon PJ, Kruse O (2004)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Schonfeld, C; Wobbe, L; Borgstadt, R; Kienast, A; Nixon, PJ; Kruse, OlafUniBi
Abstract / Bemerkung
Mitochondrial respiration plays an important role in optimizing photosynthetic efficiency in plants. As yet, the mechanisms by which plant mitochondria sense and respond to changes in the environment are unclear, particularly when exposed to light. Here we describe the characterization of the Chlamydomonas reinhardtii mutant stm6, which was identified on the basis of impaired state transitions, a mechanism that regulates light harvesting in the chloroplast. The gene disrupted in stm6, termed Moc1, encodes a homologue of the human mitochondrial transcription termination factor (mTERF). MOC1 is targeted to the mitochondrion, and its expression is up-regulated in response to light. Loss of MOC1 causes a high light-sensitive phenotype and disrupts the transcription and expression profiles of the mitochondrial respiratory complexes causing, as compared with wild type, light-mediated changes in the expression levels of nuclear and mitochondrial encoded cytochrome c oxidase subunits and ubiquinone-NAD subunits. The absence of MOC1 leads to a reduction in the levels of cytochrome c oxidase and of rotenone-insensitive external NADPH dehydrogenase activities of the mitochondrial respiratory electron transfer chain. Overall, we have identified a novel mitochondrial factor that regulates the composition of the mitochondrial respiratory chain in the light so that it can act as an effective sink for reductant produced by the chloroplast.
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Schonfeld C, Wobbe L, Borgstadt R, Kienast A, Nixon PJ, Kruse O. The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii. JOURNAL OF BIOLOGICAL CHEMISTRY. 2004;279(48):50366-50374.
Schonfeld, C., Wobbe, L., Borgstadt, R., Kienast, A., Nixon, P. J., & Kruse, O. (2004). The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii. JOURNAL OF BIOLOGICAL CHEMISTRY, 279(48), 50366-50374.
Schonfeld, C., Wobbe, L., Borgstadt, R., Kienast, A., Nixon, P. J., and Kruse, O. (2004). The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii. JOURNAL OF BIOLOGICAL CHEMISTRY 279, 50366-50374.
Schonfeld, C., et al., 2004. The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii. JOURNAL OF BIOLOGICAL CHEMISTRY, 279(48), p 50366-50374.
C. Schonfeld, et al., “The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, 2004, pp. 50366-50374.
Schonfeld, C., Wobbe, L., Borgstadt, R., Kienast, A., Nixon, P.J., Kruse, O.: The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii. JOURNAL OF BIOLOGICAL CHEMISTRY. 279, 50366-50374 (2004).
Schonfeld, C, Wobbe, L, Borgstadt, R, Kienast, A, Nixon, PJ, and Kruse, Olaf. “The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii”. JOURNAL OF BIOLOGICAL CHEMISTRY 279.48 (2004): 50366-50374.

45 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Chloroplast Acetyltransferase NSI Is Required for State Transitions in Arabidopsis thaliana.
Koskela MM, Brünje A, Ivanauskaite A, Grabsztunowicz M, Lassowskat I, Neumann U, Dinh TV, Sindlinger J, Schwarzer D, Wirtz M, Tyystjärvi E, Finkemeier I, Mulo P., Plant Cell 30(8), 2018
PMID: 29967049
Control of organelle gene expression by the mitochondrial transcription termination factor mTERF22 in Arabidopsis thaliana plants.
Shevtsov S, Nevo-Dinur K, Faigon L, Sultan LD, Zmudjak M, Markovits M, Ostersetzer-Biran O., PLoS One 13(7), 2018
PMID: 30059532
The Characterization of Arabidopsis mterf6 Mutants Reveals a New Role for mTERF6 in Tolerance to Abiotic Stress.
Robles P, Navarro-Cartagena S, Ferrández-Ayela A, Núñez-Delegido E, Quesada V., Int J Mol Sci 19(8), 2018
PMID: 30110886
Impaired Mitochondrial Transcription Termination Disrupts the Stromal Redox Poise in Chlamydomonas.
Uhmeyer A, Cecchin M, Ballottari M, Wobbe L., Plant Physiol 174(3), 2017
PMID: 28500267
Challenges and opportunities for hydrogen production from microalgae.
Oey M, Sawyer AL, Ross IL, Hankamer B., Plant Biotechnol J 14(7), 2016
PMID: 26801871
Bioaccessibility of carotenoids from Chlorella vulgaris and Chlamydomonas reinhardtii.
Gille A, Trautmann A, Posten C, Briviba K., Int J Food Sci Nutr 67(5), 2015
PMID: 27146695
Genome-wide identification, evolution and expression analysis of mTERF gene family in maize.
Zhao Y, Cai M, Zhang X, Li Y, Zhang J, Zhao H, Kong F, Zheng Y, Qiu F., PLoS One 9(4), 2014
PMID: 24718683
Increased photosystem II stability promotes H2 production in sulfur-deprived Chlamydomonas reinhardtii.
Volgusheva A, Styring S, Mamedov F., Proc Natl Acad Sci U S A 110(18), 2013
PMID: 23589846
RNAi knock-down of LHCBM1, 2 and 3 increases photosynthetic H2 production efficiency of the green alga Chlamydomonas reinhardtii.
Oey M, Ross IL, Stephens E, Steinbeck J, Wolf J, Radzun KA, Kügler J, Ringsmuth AK, Kruse O, Hankamer B., PLoS One 8(4), 2013
PMID: 23613840
Unveiling plant mTERF functions.
Robles P, Micol JL, Quesada V., Mol Plant 5(2), 2012
PMID: 22442387
Harvesting fresh water and marine algae by magnetic separation: screening of separation parameters and high gradient magnetic filtration.
Cerff M, Morweiser M, Dillschneider R, Michel A, Menzel K, Posten C., Bioresour Technol 118(), 2012
PMID: 22705536
Comparison of intact Arabidopsis thaliana leaf transcript profiles during treatment with inhibitors of mitochondrial electron transport and TCA cycle.
Umbach AL, Zarkovic J, Yu J, Ruckle ME, McIntosh L, Hock JJ, Bingham S, White SJ, George RM, Subbaiah CC, Rhoads DM., PLoS One 7(9), 2012
PMID: 23028523
Expression profile of mouse Mterfd2, a novel component of the mitochondrial transcription termination factor (MTERF) family.
Xu Q, Zhang F, He H, Xu S, Li K, Liu S, Li Y, Wu Q., Genes Genet Syst 86(4), 2011
PMID: 22214595
Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii.
Grossman AR, Catalanotti C, Yang W, Dubini A, Magneschi L, Subramanian V, Posewitz MC, Seibert M., New Phytol 190(2), 2011
PMID: 21563367
Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii.
Tolleter D, Ghysels B, Alric J, Petroutsos D, Tolstygina I, Krawietz D, Happe T, Auroy P, Adriano JM, Beyly A, Cuiné S, Plet J, Reiter IM, Genty B, Cournac L, Hippler M, Peltier G., Plant Cell 23(7), 2011
PMID: 21764992
Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development.
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL., Plant J 68(4), 2011
PMID: 21790815
Time-course global expression profiles of Chlamydomonas reinhardtii during photo-biological H₂ production.
Nguyen AV, Toepel J, Burgess S, Uhmeyer A, Blifernez O, Doebbe A, Hankamer B, Nixon P, Wobbe L, Kruse O., PLoS One 6(12), 2011
PMID: 22242116
Effects on mitochondrial transcription of manipulating mTERF protein levels in cultured human HEK293 cells.
Hyvärinen AK, Kumanto MK, Marjavaara SK, Jacobs HT., BMC Mol Biol 11(), 2010
PMID: 20846394
Phylogenetic and molecular analysis of hydrogen-producing green algae.
Timmins M, Thomas-Hall SR, Darling A, Zhang E, Hankamer B, Marx UC, Schenk PM., J Exp Bot 60(6), 2009
PMID: 19342428
The metabolome of Chlamydomonas reinhardtii following induction of anaerobic H2 production by sulfur depletion.
Matthew T, Zhou W, Rupprecht J, Lim L, Thomas-Hall SR, Doebbe A, Kruse O, Hankamer B, Marx UC, Smith SM, Schenk PM., J Biol Chem 284(35), 2009
PMID: 19478077
A mutation in the Arabidopsis mTERF-related plastid protein SOLDAT10 activates retrograde signaling and suppresses (1)O(2)-induced cell death.
Meskauskiene R, Würsch M, Laloi C, Vidi PA, Coll NS, Kessler F, Baruah A, Kim C, Apel K., Plant J 60(3), 2009
PMID: 19563435
Analytical approaches to photobiological hydrogen production in unicellular green algae.
Hemschemeier A, Melis A, Happe T., Photosynth Res 102(2-3), 2009
PMID: 19291418
Light and diurnal regulation of plant respiratory gene expression
Rasmusson AG, Escobar MA., Physiol Plant 129(1), 2007
PMID: IND43860524
Mitochondrial redox biology and homeostasis in plants.
Noctor G, De Paepe R, Foyer CH., Trends Plant Sci 12(3), 2007
PMID: 17293156
Functional integration of the HUP1 hexose symporter gene into the genome of C. reinhardtii: Impacts on biological H(2) production.
Doebbe A, Rupprecht J, Beckmann J, Mussgnug JH, Hallmann A, Hankamer B, Kruse O., J Biotechnol 131(1), 2007
PMID: 17624461
Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells.
Jin J, Davis J, Zhu D, Kashima DT, Leroueil M, Pan C, Montine KS, Zhang J., BMC Neurosci 8(), 2007
PMID: 17705834
Influence of the photoperiod on redox regulation and stress responses in Arabidopsis thaliana L. (Heynh.) plants under long- and short-day conditions.
Becker B, Holtgrefe S, Jung S, Wunrau C, Kandlbinder A, Baier M, Dietz KJ, Backhausen JE, Scheibe R., Planta 224(2), 2006
PMID: 16435132
Energy balance, organellar redox status, and acclimation to environmental stress
Wilson KE, Ivanov AG, Oquist G, Grodzinski B, Sarhan F, Huner NPA., Can J Bot 84(9), 2006
PMID: IND43864191
A family of putative transcription termination factors shared amongst metazoans and plants.
Linder T, Park CB, Asin-Cayuela J, Pellegrini M, Larsson NG, Falkenberg M, Samuelsson T, Gustafsson CM., Curr Genet 48(4), 2005
PMID: 16193327
Photosynthesis: a blueprint for solar energy capture and biohydrogen production technologies.
Kruse O, Rupprecht J, Mussgnug JH, Dismukes GC, Hankamer B., Photochem Photobiol Sci 4(12), 2005
PMID: 16307108
NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii.
Mussgnug JH, Wobbe L, Elles I, Claus C, Hamilton M, Fink A, Kahmann U, Kapazoglou A, Mullineaux CW, Hippler M, Nickelsen J, Nixon PJ, Kruse O., Plant Cell 17(12), 2005
PMID: 16284312

66 References

Daten bereitgestellt von Europe PubMed Central.

Metabolite transporters in plastids.
Flugge UI., Curr. Opin. Plant Biol. 1(3), 1998
PMID: 10066587

Arabidopsis genes encoding mitochondrial type II NAD(P)H dehydrogenases have different evolutionary origin and show distinct responses to light.
Michalecka AM, Svensson AS, Johansson FI, Agius SC, Johanson U, Brennicke A, Binder S, Rasmusson AG., Plant Physiol. 133(2), 2003
PMID: 12972666

AUTHOR UNKNOWN, Plant Biol. (N. Y.) 5(), 2003

Involvement of state transitions in the switch between linear and cyclic electron flow in Chlamydomonas reinhardtii.
Finazzi G, Rappaport F, Furia A, Fleischmann M, Rochaix JD, Zito F, Forti G., EMBO Rep. 3(3), 2002
PMID: 11850400

Mitochondrial-driven bicarbonate transport supports photosynthesis in a marine microalga.
Huertas IE, Colman B, Espie GS., Plant Physiol. 130(1), 2002
PMID: 12226508
Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration.
Cardol P, Gloire G, Havaux M, Remacle C, Matagne R, Franck F., Plant Physiol. 133(4), 2003
PMID: 14630958
Analysis of Leaf Sectors in the NCS6 Mitochondrial Mutant of Maize.
Gu J, Miles D, Newton KJ., Plant Cell 5(8), 1993
PMID: 12271093

AUTHOR UNKNOWN, Biochim. Biophys. Acta 1228(), 1995
Fluorescence and oxygen evolution from Chlorella pyrenoidosa.
Bonaventura C, Myers J., Biochim. Biophys. Acta 189(3), 1969
PMID: 5370012


Isolation and characterization of photoautotrophic mutants of Chlamydomonas reinhardtii deficient in state transition.
Fleischmann MM, Ravanel S, Delosme R, Olive J, Zito F, Wollman FA, Rochaix JD., J. Biol. Chem. 274(43), 1999
PMID: 10521495


Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.
Kindle KL, Schnell RA, Fernandez E, Lefebvre PA., J. Cell Biol. 109(6 Pt 1), 1989
PMID: 2592399

Playing tag with Chlamydomonas.
Gumpel NJ, Purton S., Trends Cell Biol. 4(8), 1994
PMID: 14768635
The PSI-H subunit of photosystem I is essential for state transitions in plant photosynthesis.
Lunde C, Jensen PE, Haldrup A, Knoetzel J, Scheller HV., Nature 408(6812), 2000
PMID: 11117752
Homologous and heterologous protein import into mitochondria isolated from the green alga Chlamydomonas reinhardtii.
Nurani G, Eriksson M, Knorpp C, Glaser E, Franzen LG., Plant Mol. Biol. 35(6), 1997
PMID: 9426617
Molecular characterization of idiA and adjacent genes in the cyanobacteria Synechococcus sp. strains PCC 6301 and PCC 7942.
Michel KP, Kruger F, Puhler A, Pistorius EK., Microbiology (Reading, Engl.) 145 ( Pt 6)(), 1999
PMID: 10411274

AUTHOR UNKNOWN, Photochem. Photobiol. 62(), 1995
A transposon with an unusual arrangement of long terminal repeats in the green alga Chlamydomonas reinhardtii.
Day A, Schirmer-Rahire M, Kuchka MR, Mayfield SP, Rochaix JD., EMBO J. 7(7), 1988
PMID: 2843359

AUTHOR UNKNOWN, J. Biol. Chem. 268(), 1993
DmTTF, a novel mitochondrial transcription termination factor that recognises two sequences of Drosophila melanogaster mitochondrial DNA.
Roberti M, Polosa PL, Bruni F, Musicco C, Gadaleta MN, Cantatore P., Nucleic Acids Res. 31(6), 2003
PMID: 12626700
Cloning and characterisation of mtDBP, a DNA-binding protein which binds two distinct regions of sea urchin mitochondrial DNA.
Loguercio Polosa P, Roberti M, Musicco C, Gadaleta MN, Quagliariello E, Cantatore P., Nucleic Acids Res. 27(8), 1999
PMID: 10101198
Organization and expression of algal (Chlamydomonas reinhardtii) mitochondrial DNA.
Gray MW, Boer PH., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 319(1193), 1988
PMID: 2901763

Assembly of cytochrome-c oxidase in cultured human cells.
Nijtmans LG, Taanman JW, Muijsers AO, Speijer D, Van den Bogert C., Eur. J. Biochem. 254(2), 1998
PMID: 9660196
State transitions, cyclic and linear electron transport and photophosphorylation in Chlamydomonas reinhardtii.
Finazzi G, Furia A, Barbagallo RP, Forti G., Biochim. Biophys. Acta 1413(3), 1999
PMID: 10556624
The Qo site of cytochrome b6f complexes controls the activation of the LHCII kinase.
Zito F, Finazzi G, Delosme R, Nitschke W, Picot D, Wollman FA., EMBO J. 18(11), 1999
PMID: 10357809
A cyclophilin-regulated PP2A-like protein phosphatase in thylakoid membranes of plant chloroplasts.
Vener AV, Rokka A, Fulgosi H, Andersson B, Herrmann RG., Biochemistry 38(45), 1999
PMID: 10555977

Beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation.
Raghavendra AS, Padmasree K., Trends Plant Sci. 8(11), 2003
PMID: 14607100
Cooperative regulation of light-harvesting complex II phosphorylation via the plastoquinol and ferredoxin-thioredoxin system in chloroplasts.
Rintamaki E, Martinsuo P, Pursiheimo S, Aro EM., Proc. Natl. Acad. Sci. U.S.A. 97(21), 2000
PMID: 11005828
The gift of Gab.
Liu Y, Rohrschneider LR., FEBS Lett. 515(1-3), 2002
PMID: 11943184
Higher plant mitochondria encode an homologue of the nuclear-encoded 30-kDa subunit of bovine mitochondrial complex I.
Lamattina L, Gonzalez D, Gualberto J, Grienenberger JM., Eur. J. Biochem. 217(3), 1993
PMID: 8223639
The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.
Stevens DR, Rochaix JD, Purton S., Mol. Gen. Genet. 251(1), 1996
PMID: 8628243


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