Sinorhizobium meliloti strain 1021 produces a low-molecular-mass capsular polysaccharide that is a homopolymer of 3-deoxy-D-manno-oct-2-ulosonic acid harboring a phospholipid anchor

Fraysse N, Lindner B, Kaczynski Z, Sharypova L, Holst O, Niehaus K, Poinsot V (2005)
Glycobiology 15(1): 101-108.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Sinorhizobium meliloti strain 1021 possesses the particularity to synthesize biologically inefficient capsular polysaccharides (KPS). It has been assumed that this class of compounds is not produced in high-molecular-mass (HMM) forms, even if many genetic analyses show the existence of expression of genes involved in the biosynthesis of capsular polysaccharides. The expression of these genes that are involved in the export of a KPS throughout the membrane and in the attachment of a lipid moiety has never been related to a structurally characterized surface polysaccharide. It is now reported that S. meliloti strain 1021 produces low-molecular-mass polysaccharides (4-4.5 kDa) that are exclusively composed of beta-(2-->7)-linked 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo) residues. These compounds are considered precursor molecules of HMM KPS, whose biosynthesis is arrested in the case of S. meliloti strain 1021. For the first time, the phospholipid anchor of a rhizobial KPS has been found, and its structure could be partially identified-namely, a phosphoglycerol moiety bearing a hydroxy-octacosanoic acid. When compared to other rhizobial KPS (composed of dimeric hexose-Kdo-like sugar repeating units), the Kdo homopolymer described here may explain why a complementation of S. meliloti strain 1021 Exo B mutant with an effective rkpZ gene restoring an active higher KPS size does not completely lead to the fully effective nitrogen fixing phenotype.
Stichworte
capsular polysaccharides; phospholipic anchor; QTOF MS; Fourier transform ion cyclotron resonance; Sinorhizobium meliloti
Erscheinungsjahr
2005
Zeitschriftentitel
Glycobiology
Band
15
Ausgabe
1
Seite(n)
101-108
ISSN
0959-6658
eISSN
1460-2423
Page URI
https://pub.uni-bielefeld.de/record/1605557

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Fraysse N, Lindner B, Kaczynski Z, et al. Sinorhizobium meliloti strain 1021 produces a low-molecular-mass capsular polysaccharide that is a homopolymer of 3-deoxy-D-manno-oct-2-ulosonic acid harboring a phospholipid anchor. Glycobiology. 2005;15(1):101-108.
Fraysse, N., Lindner, B., Kaczynski, Z., Sharypova, L., Holst, O., Niehaus, K., & Poinsot, V. (2005). Sinorhizobium meliloti strain 1021 produces a low-molecular-mass capsular polysaccharide that is a homopolymer of 3-deoxy-D-manno-oct-2-ulosonic acid harboring a phospholipid anchor. Glycobiology, 15(1), 101-108. doi:10.1093/glycob/cwh142
Fraysse, N., Lindner, B., Kaczynski, Z., Sharypova, L., Holst, O., Niehaus, K., and Poinsot, V. (2005). Sinorhizobium meliloti strain 1021 produces a low-molecular-mass capsular polysaccharide that is a homopolymer of 3-deoxy-D-manno-oct-2-ulosonic acid harboring a phospholipid anchor. Glycobiology 15, 101-108.
Fraysse, N., et al., 2005. Sinorhizobium meliloti strain 1021 produces a low-molecular-mass capsular polysaccharide that is a homopolymer of 3-deoxy-D-manno-oct-2-ulosonic acid harboring a phospholipid anchor. Glycobiology, 15(1), p 101-108.
N. Fraysse, et al., “Sinorhizobium meliloti strain 1021 produces a low-molecular-mass capsular polysaccharide that is a homopolymer of 3-deoxy-D-manno-oct-2-ulosonic acid harboring a phospholipid anchor”, Glycobiology, vol. 15, 2005, pp. 101-108.
Fraysse, N., Lindner, B., Kaczynski, Z., Sharypova, L., Holst, O., Niehaus, K., Poinsot, V.: Sinorhizobium meliloti strain 1021 produces a low-molecular-mass capsular polysaccharide that is a homopolymer of 3-deoxy-D-manno-oct-2-ulosonic acid harboring a phospholipid anchor. Glycobiology. 15, 101-108 (2005).
Fraysse, N., Lindner, B., Kaczynski, Z., Sharypova, L., Holst, O., Niehaus, Karsten, and Poinsot, V. “Sinorhizobium meliloti strain 1021 produces a low-molecular-mass capsular polysaccharide that is a homopolymer of 3-deoxy-D-manno-oct-2-ulosonic acid harboring a phospholipid anchor”. Glycobiology 15.1 (2005): 101-108.

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