The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases

Moreira LM, Hoffmann K, Albano H, Becker A, Niehaus K, Sa-Correia I (2004)
J Mol Microbiol Biotechnol 8(1): 43-57.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The high-molecular-weight exopolysaccharide gellan is an important commercial gelling agent produced in high yield by the Gram-negative bacterium Sphingomonas elodea ATCC 31461. The cluster of genes required for gellan biosynthesis contains the genes gelC and gelE. These encode for two polypeptides homologous to the activator domain and the kinase domain, respectively, of bacterial autophosphorylating tyrosine kinases involved in polysaccharide chain length determination. The GelC/GelE pair is an exception to the biochemically characterized Gram-negative tyrosine autokinases since it consists of two polypeptides instead of a single one. The deletion of gelC or gelE resulted in the abolishment of gellan in the culture medium confirming their role in gellan biosynthesis. In addition, ATP-binding assays confirmed the predicted ATP-binding ability of GelE. Interestingly, GelE contains an unusual Walker A sequence (ASTGVGCS), where the invariant lysine is replaced by a cysteine. This residue was replaced by alanine or lysine and although both mutant proteins were able to restore gellan production by complementation of the gelE deletion mutant to the production level observed with native GelE, only the mutated GelE where the cysteine was replaced by alanine was demonstrated to bind ATP in vitro. The importance of specific tyrosine residues present in the C-terminal domain of GelE in gellan assembly was also determined. The tyrosine residue at position 198 appears to be essential for the synthesis of high-molecular-weight gellan, although other tyrosine residues may additionally contribute to GelE biological function. Copyright (C) 2004 S. Karger AG, Basel.
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J Mol Microbiol Biotechnol
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8
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1
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43-57
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Moreira LM, Hoffmann K, Albano H, Becker A, Niehaus K, Sa-Correia I. The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases. J Mol Microbiol Biotechnol. 2004;8(1):43-57.
Moreira, L. M., Hoffmann, K., Albano, H., Becker, A., Niehaus, K., & Sa-Correia, I. (2004). The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases. J Mol Microbiol Biotechnol, 8(1), 43-57. doi:10.1159/000082080
Moreira, L. M., Hoffmann, K., Albano, H., Becker, A., Niehaus, K., and Sa-Correia, I. (2004). The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases. J Mol Microbiol Biotechnol 8, 43-57.
Moreira, L.M., et al., 2004. The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases. J Mol Microbiol Biotechnol, 8(1), p 43-57.
L.M. Moreira, et al., “The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases”, J Mol Microbiol Biotechnol, vol. 8, 2004, pp. 43-57.
Moreira, L.M., Hoffmann, K., Albano, H., Becker, A., Niehaus, K., Sa-Correia, I.: The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases. J Mol Microbiol Biotechnol. 8, 43-57 (2004).
Moreira, L. M., Hoffmann, K., Albano, H., Becker, A., Niehaus, Karsten, and Sa-Correia, I. “The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases”. J Mol Microbiol Biotechnol 8.1 (2004): 43-57.

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

The evolutionary life cycle of the polysaccharide biosynthetic gene cluster based on the Sphingomonadaceae.
Wu M, Huang H, Li G, Ren Y, Shi Z, Li X, Dai X, Gao G, Ren M, Ma T., Sci Rep 7(), 2017
PMID: 28429731
Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.
Schmid J, Sieber V, Rehm B., Front Microbiol 6(), 2015
PMID: 26074894
Microbial protein-tyrosine kinases.
Chao JD, Wong D, Av-Gay Y., J Biol Chem 289(14), 2014
PMID: 24554699
A comparison of genes involved in sphingan biosynthesis brought up to date.
Schmid J, Sperl N, Sieber V., Appl Microbiol Biotechnol 98(18), 2014
PMID: 25081553
Identification and organization of genes for diutan polysaccharide synthesis from Sphingomonas sp. ATCC 53159.
Coleman RJ, Patel YN, Harding NE., J Ind Microbiol Biotechnol 35(4), 2008
PMID: 18210176
Occurrence, production, and applications of gellan: current state and perspectives.
Fialho AM, Moreira LM, Granja AT, Popescu AO, Hoffmann K, Sá-Correia I., Appl Microbiol Biotechnol 79(6), 2008
PMID: 18506441

56 References

Daten bereitgestellt von Europe PubMed Central.

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ., Nucleic Acids Res. 25(17), 1997
PMID: 9254694
The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products.
Becker A, Ruberg S, Kuster H, Roxlau AA, Keller M, Ivashina T, Cheng HP, Walker GC, Puhler A., J. Bacteriol. 179(4), 1997
PMID: 9023225

Bullock, Biotechniques 5(), 1987
Protein phosphorylation on tyrosine in bacteria.
Cozzone AJ, Grangeasse C, Doublet P, Duclos B., Arch. Microbiol. 181(3), 2004
PMID: 14745484
Structural organization of the protein-tyrosine autokinase Wzc within Escherichia coli cells.
Doublet P, Grangeasse C, Obadia B, Vaganay E, Cozzone AJ., J. Biol. Chem. 277(40), 2002
PMID: 12138098
On the binding of ATP to the autophosphorylating protein, Ptk, of the bacterium Acinetobacter johnsonii.
Doublet P, Vincent C, Grangeasse C, Cozzone AJ, Duclos B., FEBS Lett. 445(1), 1999
PMID: 10069388
Autophosphorylation of a bacterial protein at tyrosine.
Duclos B, Grangeasse C, Vaganay E, Riberty M, Cozzone AJ., J. Mol. Biol. 259(5), 1996
PMID: 8683591
Characterization of a bacterial gene encoding an autophosphorylating protein tyrosine kinase.
Grangeasse C, Doublet P, Vaganay E, Vincent C, Deleage G, Duclos B, Cozzone AJ., Gene 204(1-2), 1997
PMID: 9434192
Functional characterization of the low-molecular-mass phosphotyrosine-protein phosphatase of Acinetobacter johnsonii.
Grangeasse C, Doublet P, Vincent C, Vaganay E, Riberty M, Duclos B, Cozzone AJ., J. Mol. Biol. 278(2), 1998
PMID: 9571056
Organization of genes required for gellan polysaccharide biosynthesis in Sphingomonas elodea ATCC 31461.
Harding NE, Patel YN, Coleman RJ., J. Ind. Microbiol. Biotechnol. 31(2), 2004
PMID: 14767675
Protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysaccharide.
Ilan O, Bloch Y, Frankel G, Ullrich H, Geider K, Rosenshine I., EMBO J. 18(12), 1999
PMID: 10369665

AUTHOR UNKNOWN, 0

Kang, US Patent 4(), 1981

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
pBBR1MCS: a broad-host-range cloning vector.
Kovach ME, Phillips RW, Elzer PH, Roop RM 2nd, Peterson KM., BioTechniques 16(5), 1994
PMID: 8068328
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A, Larsson B, von Heijne G, Sonnhammer EL., J. Mol. Biol. 305(3), 2001
PMID: 11152613
Gellan gum biosynthetic enzymes in producing and nonproducing variants of Pseudomonas elodea.
Martins LO, Sa-Correia I., Biotechnol. Appl. Biochem. 14(3), 1991
PMID: 1777120
Identification and physical organization of the gene cluster involved in the biosynthesis of Burkholderia cepacia complex exopolysaccharide.
Moreira LM, Videira PA, Sousa SA, Leitao JH, Cunha MV, Sa-Correia I., Biochem. Biophys. Res. Commun. 312(2), 2003
PMID: 14637140
Translocation of group 1 capsular polysaccharide in Escherichia coli serotype K30. Structural and functional analysis of the outer membrane lipoprotein Wza.
Nesper J, Hill CM, Paiment A, Harauz G, Beis K, Naismith JH, Whitfield C., J. Biol. Chem. 278(50), 2003
PMID: 14522970

AUTHOR UNKNOWN, 0
Computer-based analyses of the protein constituents of transport systems catalysing export of complex carbohydrates in bacteria.
Paulsen IT, Beness AM, Saier MH Jr., Microbiology (Reading, Engl.) 143 ( Pt 8)(), 1997
PMID: 9274022
Isolation and characterization of a protein-tyrosine kinase and a phosphotyrosine-protein phosphatase from Klebsiella pneumoniae.
Preneta R, Jarraud S, Vincent C, Doublet P, Duclos B, Etienne J, Cozzone AJ., Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 131(1), 2002
PMID: 11742763

AUTHOR UNKNOWN, 0
Gellan gum biosynthesis in Sphingomonas paucimobilis ATCC 31461: genes, enzymes and exopolysaccharide production engineering.
Sa-Correia I, Fialho AM, Videira P, Moreira LM, Marques AR, Albano H., J. Ind. Microbiol. Biotechnol. 29(4), 2002
PMID: 12355314

AUTHOR UNKNOWN, 0

Simon, Methods Enzymol. 1118(), 1986

Towbin, Proc. Nat. Acad. Sci. USA 76(), 1979
Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in gram-negative bacteria.
Vincent C, Duclos B, Grangeasse C, Vaganay E, Riberty M, Cozzone AJ, Doublet P., J. Mol. Biol. 304(3), 2000
PMID: 11090276

Vincent, Wzb. J. Bacteriol. 181(), 1999
Structure, assembly and regulation of expression of capsules in Escherichia coli.
Whitfield C, Roberts IS., Mol. Microbiol. 31(5), 1999
PMID: 10200953
Phosphorylation of Wzc, a tyrosine autokinase, is essential for assembly of group 1 capsular polysaccharides in Escherichia coli.
Wugeditsch T, Paiment A, Hocking J, Drummelsmith J, Forrester C, Whitfield C., J. Biol. Chem. 276(4), 2000
PMID: 11053445
Linkage of genes essential for synthesis of a polysaccharide capsule in Sphingomonas strain S88.
Yamazaki M, Thorne L, Mikolajczak M, Armentrout RW, Pollock TJ., J. Bacteriol. 178(9), 1996
PMID: 8626338
Genes involved in the synthesis of the exopolysaccharide methanolan by the obligate methylotroph Methylobacillus sp strain 12S.
Yoshida T, Ayabe Y, Yasunaga M, Usami Y, Habe H, Nojiri H, Omori T., Microbiology (Reading, Engl.) 149(Pt 2), 2003
PMID: 12624205

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