High magnitude of light retardation by the zona pellucida is associated with conception cycles

Shen Y, Stalf T, Mehnert C, Eichenlaub-Ritter U, Tinneberg HR (2005)
HUMAN REPRODUCTION 20(6): 1596-1606.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Shen, Y; Stalf, T; Mehnert, C; Eichenlaub-Ritter, UrsulaUniBi; Tinneberg, HR
Abstract / Bemerkung
BACKGROUND: Failures in expression of zona proteins correlate to subfertility in animals. Low expression of the zona proteins by the growing human oocyte may indicate reduced developmental potential. Therefore, we non-invasively analysed the thickness and the structure of the zona pellucida (ZP) of human oocytes with respect to embryo fate after ICSI. METHODS: Retardance magnitude and thickness of the inner, middle and outer layers of the ZP were quantitatively analysed by a Polscope in 166 oocytes selected for transfer after ICSI (63 patients; 32.8 +/- 4.4 years) on the basis of pronuclear score at day 1. Blastomere number was determined at day 2. Data were compared between conception cycles (CC; 65 oocytes/23 patients) and non-conception cycles (NCC; 101 oocytes/40 patients) and with respect to maternal age. RESULTS: The thickness was slightly elevated (P < 0.001), and the mean magnitude of light retardance was nearly 30% higher (P < 0.001) in the inner layer of the zona pellucida of oocytes contributing to CC compared to NCC. Embryos in the CC group tended to develop faster. CONCLUSIONS: The magnitude of light retardance by the zona pellucida inner layer appears to present a unique non-invasive marker for oocyte developmental potential.
human oocyte; conception; ICSI; zona pellucida; Polscope
Page URI


Shen Y, Stalf T, Mehnert C, Eichenlaub-Ritter U, Tinneberg HR. High magnitude of light retardation by the zona pellucida is associated with conception cycles. HUMAN REPRODUCTION. 2005;20(6):1596-1606.
Shen, Y., Stalf, T., Mehnert, C., Eichenlaub-Ritter, U., & Tinneberg, H. R. (2005). High magnitude of light retardation by the zona pellucida is associated with conception cycles. HUMAN REPRODUCTION, 20(6), 1596-1606. https://doi.org/10.1093/humrep/deh811
Shen, Y, Stalf, T, Mehnert, C, Eichenlaub-Ritter, Ursula, and Tinneberg, HR. 2005. “High magnitude of light retardation by the zona pellucida is associated with conception cycles”. HUMAN REPRODUCTION 20 (6): 1596-1606.
Shen, Y., Stalf, T., Mehnert, C., Eichenlaub-Ritter, U., and Tinneberg, H. R. (2005). High magnitude of light retardation by the zona pellucida is associated with conception cycles. HUMAN REPRODUCTION 20, 1596-1606.
Shen, Y., et al., 2005. High magnitude of light retardation by the zona pellucida is associated with conception cycles. HUMAN REPRODUCTION, 20(6), p 1596-1606.
Y. Shen, et al., “High magnitude of light retardation by the zona pellucida is associated with conception cycles”, HUMAN REPRODUCTION, vol. 20, 2005, pp. 1596-1606.
Shen, Y., Stalf, T., Mehnert, C., Eichenlaub-Ritter, U., Tinneberg, H.R.: High magnitude of light retardation by the zona pellucida is associated with conception cycles. HUMAN REPRODUCTION. 20, 1596-1606 (2005).
Shen, Y, Stalf, T, Mehnert, C, Eichenlaub-Ritter, Ursula, and Tinneberg, HR. “High magnitude of light retardation by the zona pellucida is associated with conception cycles”. HUMAN REPRODUCTION 20.6 (2005): 1596-1606.

42 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Developmental potential of embryos from cycles containing oocytes with severe ovoid zona pellucida.
Xiong S, Han W, Liu W, Wu L, Liu JX, Gao Y, Huang G., Hum Fertil (Camb) 21(3), 2018
PMID: 28545306
Evaluation of equine oocyte developmental competence using polarized light microscopy.
Bertero A, Ritrovato F, Evangelista F, Stabile V, Fortina R, Ricci A, Revelli A, Vincenti L, Nervo T., Reproduction 153(6), 2017
PMID: 28283673
Zona pellucida gene mRNA expression in human oocytes is related to oocyte maturity, zona inner layer retardance and fertilization competence.
Canosa S, Adriaenssens T, Coucke W, Dalmasso P, Revelli A, Benedetto C, Smitz J., Mol Hum Reprod 23(5), 2017
PMID: 28204536
Noninvasive imaging systems for gametes and embryo selection in IVF programs: a review.
Omidi M, Faramarzi A, Agharahimi A, Khalili MA., J Microsc 267(3), 2017
PMID: 28470749
Novel zona pellucida gene variants identified in patients with oocyte anomalies.
Yang P, Luan X, Peng Y, Chen T, Su S, Zhang C, Wang Z, Cheng L, Zhang X, Wang Y, Chen ZJ, Zhao H., Fertil Steril 107(6), 2017
PMID: 28577617
Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI.
Revelli A, Canosa S, Bergandi L, Skorokhod OA, Biasoni V, Carosso A, Bertagna A, Maule M, Aldieri E, D'Eufemia MD, Evangelista F, Colacurci N, Benedetto C., Reprod Biol Endocrinol 15(1), 2017
PMID: 28645283
Embryological, clinical and ultrastructural study of human oocytes presenting indented zona pellucida.
Sousa M, Teixeira da Silva J, Silva J, Cunha M, Viana P, Oliveira E, Sá R, Soares C, Oliveira C, Barros A., Zygote 23(1), 2015
PMID: 23992046
Cleavage pattern predicts developmental potential of day 3 human embryos produced by IVF.
Yang ST, Shi JX, Gong F, Zhang SP, Lu CF, Tan K, Leng LZ, Hao M, He H, Gu YF, Lu GX, Lin G., Reprod Biomed Online 30(6), 2015
PMID: 25892500
Fertilization and embryo quality of mature oocytes with specific morphological abnormalities.
Yu EJ, Ahn H, Lee JM, Jee BC, Kim SH., Clin Exp Reprod Med 42(4), 2015
PMID: 26815385
The root of reduced fertility in aged women and possible therapentic options: current status and future perspects.
Qiao J, Wang ZB, Feng HL, Miao YL, Wang Q, Yu Y, Wei YC, Yan J, Wang WH, Shen W, Sun SC, Schatten H, Sun QY., Mol Aspects Med 38(), 2014
PMID: 23796757
Oocyte morphology on day 0 correlates with aneuploidy as detected by polar body biopsy and FISH.
Schmutzler AG, Acar-Perk B, Weimer J, Salmassi A, Sievers K, Tobler M, Mettler L, Jonat W, Arnold N., Arch Gynecol Obstet 289(2), 2014
PMID: 23880887
Are zona pellucida genes involved in recurrent oocyte lysis observed during in vitro fertilization?
Ferré M, Amati-Bonneau P, Morinière C, Ferré-L'Hôtellier V, Lemerle S, Przyrowski D, Procaccio V, Descamps P, Reynier P, May-Panloup P., J Assist Reprod Genet 31(2), 2014
PMID: 24242990
Oocytes with a dark zona pellucida demonstrate lower fertilization, implantation and clinical pregnancy rates in IVF/ICSI cycles.
Shi W, Xu B, Wu LM, Jin RT, Luan HB, Luo LH, Zhu Q, Johansson L, Liu YS, Tong XH., PLoS One 9(2), 2014
PMID: 24586757
Developmental competence of equine oocytes: impacts of zona pellucida birefringence and maternally derived transcript expression.
Mohammadi-Sangcheshmeh A, Held E, Rings F, Ghanem N, Salilew-Wondim D, Tesfaye D, Sieme H, Schellander K, Hoelker M., Reprod Fertil Dev 26(3), 2014
PMID: 23622680
Optimizing TESE-ICSI by laser-assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes.
Nordhoff V, Schüring AN, Krallmann C, Zitzmann M, Schlatt S, Kiesel L, Kliesch S., Andrology 1(1), 2013
PMID: 23258632
Quantitative polarized light microscopy of unstained mammalian cochlear sections.
Kalwani NM, Ong CA, Lysaght AC, Haward SJ, McKinley GH, Stankovic KM., J Biomed Opt 18(2), 2013
PMID: 23407909
Zona pellucida birefringence correlates with developmental capacity of bovine oocytes classified by maturational environment, COC morphology and G6PDH activity.
Held E, Mertens EM, Mohammadi-Sangcheshmeh A, Salilew-Wondim D, Besenfelder U, Havlicek V, Herrler A, Tesfaye D, Schellander K, Hölker M., Reprod Fertil Dev 24(4), 2012
PMID: 22541545
The oocyte.
Rienzi L, Balaban B, Ebner T, Mandelbaum J., Hum Reprod 27 Suppl 1(), 2012
PMID: 22811312
Polarized light microscopy-detectable structures of human oocytes and embryos are related to the likelihood of conception in IVF.
Molinari E, Evangelista F, Racca C, Cagnazzo C, Revelli A., J Assist Reprod Genet 29(10), 2012
PMID: 22895811
Predictive value of oocyte morphology in human IVF: a systematic review of the literature.
Rienzi L, Vajta G, Ubaldi F., Hum Reprod Update 17(1), 2011
PMID: 20639518
Sequence variations in human ZP genes as potential modifiers of zona pellucida architecture.
Pökkylä RM, Lakkakorpi JT, Nuojua-Huttunen SH, Tapanainen JS., Fertil Steril 95(8), 2011
PMID: 21334611
Evaluation of bovine zona pellucida characteristics in polarized light as a prognostic marker for embryonic developmental potential.
Koester M, Mohammadi-Sangcheshmeh A, Montag M, Rings F, Schimming T, Tesfaye D, Schellander K, Hoelker M., Reproduction 141(6), 2011
PMID: 21415090
Correlation of oocyte morphometry parameters with woman's age.
Valeri C, Pappalardo S, De Felici M, Manna C., J Assist Reprod Genet 28(6), 2011
PMID: 21468654
Evaluation of zona pellucida birefringence intensity during in vitro maturation of oocytes from stimulated cycles.
Petersen CG, Vagnini LD, Mauri AL, Massaro FC, Silva LF, Cavagna M, Baruffi RL, Oliveira JB, Franco JG., Reprod Biol Endocrinol 9(), 2011
PMID: 21513548
Gamete competence assessment by polarizing optics in assisted reproduction.
Montag M, Köster M, van der Ven K, van der Ven H., Hum Reprod Update 17(5), 2011
PMID: 21531752
A review of the promises and pitfalls of oocyte and embryo metabolomics.
Nel-Themaat L, Nagy ZP., Placenta 32 Suppl 3(), 2011
PMID: 21703683
Automatic user-independent zona pellucida imaging at the oocyte stage allows for the prediction of preimplantation development.
Ebner T, Balaban B, Moser M, Shebl O, Urman B, Ata B, Tews G., Fertil Steril 94(3), 2010
PMID: 19439291
Cumulative results including obstetrical and neonatal outcome of fresh and frozen-thawed cycles in elective single versus double fresh embryo transfers.
Fauque P, Jouannet P, Davy C, Guibert J, Viallon V, Epelboin S, Kunstmann JM, Patrat C., Fertil Steril 94(3), 2010
PMID: 19446806
Quantitative analysis of the intensity of zona pellucida birefringence of oocytes during IVF cycles.
Cheng J, Huang L, He B, Lu F, Wang X, Wu Z, Shi D., Reprod Fertil Dev 22(8), 2010
PMID: 20883641
Slow-freezing-induced changes of birefringent structures in human oocytes are related to responsiveness to ovulation induction.
Molinari E, Revelli A, Racca C, Delle Piane L, Massobrio M., Reprod Biomed Online 20(5), 2010
PMID: 20219427
Polarized light microscopy in mammalian oocytes.
Caamaño JN, Muñoz M, Diez C, Gómez E., Reprod Domest Anim 45 Suppl 2(), 2010
PMID: 20591065
Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes.
Shen Y, Betzendahl I, Tinneberg HR, Eichenlaub-Ritter U., Mutat Res 651(1-2), 2008
PMID: 18160331
Phase-subtraction cell-counting method for live mouse embryos beyond the eight-cell stage.
Warger WC, Newmark JA, Warner CM, DiMarzio CA., J Biomed Opt 13(3), 2008
PMID: 18601550
Release of sICAM-1 in oocytes and in vitro fertilized human embryos.
Borgatti M, Rizzo R, Canto MB, Fumagalli D, Renzini MM, Fadini R, Stignani M, Baricordi OR, Gambari R., PLoS One 3(12), 2008
PMID: 19092999
A randomized double-blind controlled study on the efficacy of laser zona pellucida thinning on live birth rates in cases of advanced female age.
Frydman N, Madoux S, Hesters L, Duvernoy C, Feyereisen E, Le Du A, Tachdjian G, Frydman R, Fanchin R., Hum Reprod 21(8), 2006
PMID: 16644913

88 References

Daten bereitgestellt von Europe PubMed Central.

Patterns of intercellular connectivity in the mammalian cumulus-oocyte complex.
Albertini DF, Rider V., Microsc. Res. Tech. 27(2), 1994
PMID: 8123905
Pronuclear morphology predicts embryo development and chromosome constitution.
Balaban B, Yakin K, Urman B, Isiklar A, Tesarik J., Reprod. Biomed. Online 8(6), 2004
PMID: 15169588
Fine structure of the zona pellucida of unfertilized egg cells and embryos.
Baranska W, Konwinski M, Kujawa M., J. Exp. Zool. 192(2), 1975
PMID: 1133567
Composition of human zona pellucida as revealed by SDS-PAGE after silver staining.
Bercegeay S, Jean M, Lucas H, Barriere P., Mol. Reprod. Dev. 41(3), 1995
PMID: 8588935

Localization and synthesis of zona pellucida proteins in the marmoset monkey (Callithrix jacchus) ovary.
Bogner K, Hinsch KD, Nayudu P, Konrad L, Cassara C, Hinsch E., Mol. Hum. Reprod. 10(7), 2004
PMID: 15169921
Localization of species conserved zona pellucida antigens in mammalian ovaries.
Carino C, Prasad S, Skinner S, Dunbar B, Chirinos M, Schwoebel E, Larrea F, Dunbar B., Reprod. Biomed. Online 4(2), 2002
PMID: 12470573
A prospective randomized controlled study of the effect of short coincubation of gametes during insemination on zona pellucida thickness.
Dirnfeld M, Shiloh H, Bider D, Harari E, Koifman M, Lahav-Baratz S, Abramovici H., Gynecol. Endocrinol. 17(5), 2003
PMID: 14710587
Immunohistochemical localization of zona pellucida proteins ZPA, ZPB and ZPC in human, cynomolgus monkey and mouse ovaries.
Eberspaecher U, Becker A, Bringmann P, van der Merwe L, Donner P., Cell Tissue Res. 303(2), 2001
PMID: 11291774
Manipulation of the oocyte: possible damage to the spindle apparatus.
Eichenlaub-Ritter U, Shen Y, Tinneberg HR., Reprod. Biomed. Online 5(2), 2002
PMID: 12419035

Human zona pellucida during in vitro fertilization: an ultrastructural study using saponin, ruthenium red, and osmium-thiocarbohydrazide.
Familiari G, Nottola SA, Macchiarelli G, Micara G, Aragona C, Motta PM., Mol. Reprod. Dev. 32(1), 1992
PMID: 1381198

Alternative approaches in IVF.
Fauser BC, Bouchard P, Coelingh Bennink HJ, Collins JA, Devroey P, Evers JL, van Steirteghem A., Hum. Reprod. Update 8(1), 2002
PMID: 11871382

The effect of pronuclear morphology on early development and chromosomal abnormalities in cleavage-stage embryos.
Gamiz P, Rubio C, de los Santos MJ, Mercader A, Simon C, Remohi J, Pellicer A., Hum. Reprod. 18(11), 2003
PMID: 14585895
Pronuclear morphology and chromosomal abnormalities as scoring criteria for embryo selection.
Gianaroli L, Magli MC, Ferraretti AP, Fortini D, Grieco N., Fertil. Steril. 80(2), 2003
PMID: 12909497



Three-dimensional structure of the zona pellucida.
Green DP., Rev. Reprod. 2(3), 1997
PMID: 9414478
Early embryonic coats: morphology, function, practical applications. An overview.
Herrler A, Beier HM., Cells Tissues Organs (Print) 166(2), 2000
PMID: 10729731
Embryo-maternal signalling: how the embryo starts talking to its mother to accomplish implantation.
Herrler A, von Rango U, Beier HM., Reprod. Biomed. Online 6(2), 2003
PMID: 12676010
Insights into the molecular basis of sperm-egg recognition in mammals.
Hoodbhoy T, Dean J., Reproduction 127(4), 2004
PMID: 15047932

Carbohydrate analysis of the zona pellucida and cortical granules of human oocytes by means of ultrastructural cytochemistry.
Jimenez-Movilla M, Aviles M, Gomez-Torres MJ, Fernandez-Colom PJ, Castells MT, de Juan J, Romeu A, Ballesta J., Hum. Reprod. 19(8), 2004
PMID: 15218004
Imaging meiotic spindles by polarization light microscopy: principles and applications to IVF.
Keefe D, Liu L, Wang W, Silva C., Reprod. Biomed. Online 7(1), 2003
PMID: 12930570

Chromosomal abnormalities in a series of 6,733 human oocytes in preimplantation diagnosis for age-related aneuploidies.
Kuliev A, Cieslak J, Ilkevitch Y, Verlinsky Y., Reprod. Biomed. Online 6(1), 2003
PMID: 12626143

Four zona pellucida glycoproteins are expressed in the human.
Lefievre L, Conner SJ, Salpekar A, Olufowobi O, Ashton P, Pavlovic B, Lenton W, Afnan M, Brewis IA, Monk M, Hughes DC, Barratt CL., Hum. Reprod. 19(7), 2004
PMID: 15142998
Targeted disruption of the mZP3 gene results in production of eggs lacking a zona pellucida and infertility in female mice.
Liu C, Litscher ES, Mortillo S, Sakai Y, Kinloch RA, Stewart CL, Wassarman PM., Proc. Natl. Acad. Sci. U.S.A. 93(11), 1996
PMID: 8643592
Early embryo cleavage is a strong indicator of embryo quality in human IVF.
Lundin K, Bergh C, Hardarson T., Hum. Reprod. 16(12), 2001
PMID: 11726590


Composition of the human zona pellucida and modifications following fertilization.
Moos J, Faundes D, Kopf GS, Schultz RM., Hum. Reprod. 10(9), 1995
PMID: 8530692

Differences in chromosome susceptibility to aneuploidy and survival to first trimester.
Munne S, Bahce M, Sandalinas M, Escudero T, Marquez C, Velilla E, Colls P, Oter M, Alikani M, Cohen J., Reprod. Biomed. Online 8(1), 2004
PMID: 14759293
Pronuclear morphology evaluation with subsequent evaluation of embryo morphology significantly increases implantation rates.
Nagy ZP, Dozortsev D, Diamond M, Rienzi L, Ubaldi F, Abdelmassih R, Greco E., Fertil. Steril. 80(1), 2003
PMID: 12849803
The zona reaction in human oocytes as seen with scanning electron microscopy.
Nikas G, Paraschos T, Psychoyos A, Handyside AH., Hum. Reprod. 9(11), 1994
PMID: 7868686
Biochemical and functional characterization of the human zona pellucida.
Oehninger S., Reprod. Biomed. Online 7(6), 2003
PMID: 14748962
A new view on polarization microscopy.
Oldenbourg R., Nature 381(6585), 1996
PMID: 8657288

Surface properties of the zona pellucida.
Phillips DM, Shalgi RM., J. Exp. Zool. 213(1), 1980
PMID: 7452192
Structure and function of the proteins of the mammalian Zona pellucida.
Prasad SV, Skinner SM, Carino C, Wang N, Cartwright J, Dunbar BS., Cells Tissues Organs (Print) 166(2), 2000
PMID: 10729725



Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI.
Rienzi L, Ubaldi F, Martinez F, Iacobelli M, Minasi MG, Ferrero S, Tesarik J, Greco E., Hum. Reprod. 18(6), 2003
PMID: 12773461
Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures.
Rienzi L, Martinez F, Ubaldi F, Minasi MG, Iacobelli M, Tesarik J, Greco E., Hum. Reprod. 19(3), 2004
PMID: 14998966

Non-invasive method to assess genotoxicity of nocodazole interfering with spindle formation in mammalian oocytes.
Shen Y, Betzendahl I, Sun F, Tinneberg HR, Eichenlaub-Ritter U., Reprod. Toxicol. 19(4), 2005
PMID: 15749259
The impact of cigarette smoking on zona pellucida thickness of oocytes and embryos prior to transfer into the uterine cavity.
Shiloh H, Lahav-Baratz S, Koifman M, Ishai D, Bidder D, Weiner-Meganzi Z, Dirnfeld M., Hum. Reprod. 19(1), 2004
PMID: 14688175
Functional morphology of the zona pellucida.
Sinowatz F, Topfer-Petersen E, Kolle S, Palma G., Anat Histol Embryol 30(5), 2001
PMID: 11688734

Evolution and nomenclature of the zona pellucida gene family.
Spargo SC, Hope RM., Biol. Reprod. 68(2), 2003
PMID: 12533396
Influence of polarization effects in ooplasma and pronuclei on embryo quality and implantation in an IVF program.
Stalf T, Herrero J, Mehnert C, Manolopoulos K, Lenhard A, Gips H., J. Assist. Reprod. Genet. 19(8), 2002
PMID: 12182441
Pregnancy following fertilization of zona-free, coronal cell intact human ova: Case Report.
Stanger JD, Stevenson K, Lakmaker A, Woolcott R., Hum. Reprod. 16(1), 2001
PMID: 11139556

Normal development of a zona-free oocyte to the blastocyst stage following ICSI.
Takahashi K, Araki Y, Motoyama M., Hum. Reprod. 14(10), 1999
PMID: 10528275
Expression of recombinant human zona pellucida protein 2 and its binding capacity to spermatozoa.
Tsubamoto H, Hasegawa A, Nakata Y, Naito S, Yamasaki N, Koyama K., Biol. Reprod. 61(6), 1999
PMID: 10570015
Structural aspects of the zona pellucida of in vitro-produced bovine embryos: a scanning electron and confocal laser scanning microscopic study.
Vanroose G, Nauwynck H, Soom AV, Ysebaert MT, Charlier G, Oostveldt PV, de Kruif A., Biol. Reprod. 62(2), 2000
PMID: 10642588
Zona pellucida glycoproteins.
Wassarman PM., Annu. Rev. Biochem. 57(), 1988
PMID: 3052278

Structure and function of the mammalian egg zona pellucida.
Wassarman P, Chen J, Cohen N, Litscher E, Liu C, Qi H, Williams Z., J. Exp. Zool. 285(3), 1999
PMID: 10497324


FISH analysis for chromosomes 13, 16, 18, 21, 22, X and Y in all blastomeres of IVF pre-embryos from 144 randomly selected donated human oocytes and impact on pre-embryo morphology.
Ziebe S, Lundin K, Loft A, Bergh C, Nyboe Andersen A, Selleskog U, Nielsen D, Grondahl C, Kim H, Arce JC; CEMAS II and Study Group., Hum. Reprod. 18(12), 2003
PMID: 14645173
The use of a detailed zygote score after IVF/ICSI to obtain good quality blastocysts: the German experience.
Zollner U, Zollner KP, Hartl G, Dietl J, Steck T., Hum. Reprod. 17(5), 2002
PMID: 11980760

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

PMID: 15734754
PubMed | Europe PMC

Suchen in

Google Scholar