Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis
Burger A, Grafen I, Engemann J, Niermann E, Pieper M, Kirchner O, Gartemann K-H, Eichenlaub R (2005)
MICROBIOLOGICAL RESEARCH 160(4): 417-427.
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Autor*in
Burger, A;
Grafen, I;
Engemann, J;
Niermann, E;
Pieper, M;
Kirchner, O;
Gartemann, Karl-HeinzUniBi
;
Eichenlaub, RudolfUniBi
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Abstract / Bemerkung
Hybridization of Clavibacter michiganensis subsp. michiganensis total DNA against the pathogenicity gene pat-1 indicated the presence of pat-1 homologous nucteotide sequences on the chromosome and on plasmid pCM2. Isolation of the corresponding DNA fragments and nucleotide sequence determination showed that there are three pat-1 homologous genes: chpA (chromosome) and phpA and phpB (plasmid pCM2). The gene products share common characteristics, i.e. a signal sequence for Sec-dependent secretion, a serine protease motif, and six cysteine residues at conserved positions. Gene chpA located on the chromosome is a pseudogene since it contains a translational stop codon after 97 of 280 amino acids. In contrast to pat-1, cloning of the plasmid encoded homologs phpA and phpB into the avirulent plasmid free Cmm strain CMM100 did not result in a virulent phenotype. So far no proteolytic activity could be demonstrated for Pat-1, however, site specific mutagenesis of pat-1 showed that the serine residue in the motif GDSGG is required for the virulent phenotype of pat-1 and thus Pat-1 could be a functional protease. (c) 2005 Elsevier GmbH. All rights reserved.
Stichworte
Clavibacter;
phytopathogen;
serine protease
Erscheinungsjahr
2005
Zeitschriftentitel
MICROBIOLOGICAL RESEARCH
Band
160
Ausgabe
4
Seite(n)
417-427
ISSN
0944-5013
Page URI
https://pub.uni-bielefeld.de/record/1601694
Zitieren
Burger A, Grafen I, Engemann J, et al. Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis. MICROBIOLOGICAL RESEARCH. 2005;160(4):417-427.
Burger, A., Grafen, I., Engemann, J., Niermann, E., Pieper, M., Kirchner, O., Gartemann, K. - H., et al. (2005). Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis. MICROBIOLOGICAL RESEARCH, 160(4), 417-427. https://doi.org/10.1016/j.micres.2005.03.006
Burger, A, Grafen, I, Engemann, J, Niermann, E, Pieper, M, Kirchner, O, Gartemann, Karl-Heinz, and Eichenlaub, Rudolf. 2005. “Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis”. MICROBIOLOGICAL RESEARCH 160 (4): 417-427.
Burger, A., Grafen, I., Engemann, J., Niermann, E., Pieper, M., Kirchner, O., Gartemann, K. - H., and Eichenlaub, R. (2005). Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis. MICROBIOLOGICAL RESEARCH 160, 417-427.
Burger, A., et al., 2005. Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis. MICROBIOLOGICAL RESEARCH, 160(4), p 417-427.
A. Burger, et al., “Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis”, MICROBIOLOGICAL RESEARCH, vol. 160, 2005, pp. 417-427.
Burger, A., Grafen, I., Engemann, J., Niermann, E., Pieper, M., Kirchner, O., Gartemann, K.-H., Eichenlaub, R.: Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis. MICROBIOLOGICAL RESEARCH. 160, 417-427 (2005).
Burger, A, Grafen, I, Engemann, J, Niermann, E, Pieper, M, Kirchner, O, Gartemann, Karl-Heinz, and Eichenlaub, Rudolf. “Identification of homologues to the pathogenicity factor pat-1, a putative serine protease of Clavibacter michiganensis subsp michiganensis”. MICROBIOLOGICAL RESEARCH 160.4 (2005): 417-427.
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