A close look at fluorescence quenching of organic dyes by tryptophan
Doose S, Neuweiler H, Sauer M (2005)
CHEMPHYSCHEM 6(11): 2277-2285.
Zeitschriftenaufsatz
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Autor*in
Doose, S;
Neuweiler, H;
Sauer, MarkusUniBi
Einrichtung
Abstract / Bemerkung
Understanding fluorescence quenching processes of organic dyes by biomolecular compounds is of fundamental importance for in-vitro and in-vivo fluorescence studies. It has been reported that the excited singlet state of some oxazine and rhodamine derivatives is efficiently and almost exclusively quenched by the amino acid tryptophan (Trp) and the DNA base guanine via photoinduced electron transfer (PET). We present a detailed analysis of the quenching interactions between the oxazine dye MR121 and Trp in aqueous buffer. Steady-state and time-resolved fluorescence spectroscopy, together with fluorescence correlation spectroscopy (FCS), reveal three contributing quenching mechanisms: 1) diffusion-limited dynamic quenching with a bimolecular quenching rate constant k(d) of 4.0 x 10(9) s(-1) M-1, 2) static quenching with a bimolecular association constant K-s of 61 M-1, and 3) a sphere-of-action contribution to static quenching described by an exponential factor with a quenching constant lambda of 22 M-1. The latter two are characterized as nonfluorescent complexes, formed with approximate to 30% efficiency upon encounter, that are stable for tens of nanoseconds. The measured binding energy of 2030 kJmol(-1) is consistent with previous estimates from molecular dynamics simulations that proposed stacked complexes due to hydrophobic forces. We further evaluate the influence of glycerol and denaturant (guanidine hydrochloride) on the formation and stability of quenched complexes. Comparative measurements performed with two other dyes, ATTO 655 and Rhodamine 6G show similar results and thus demonstrate the general applicability of utilizing PET between organic dyes and Trp for the study of conformational dynamics of biopolymers on sub-nanometer length and nanosecond time-scales.
Stichworte
fluorescence spectroscopy;
electron transfer;
tryptophan;
dyes;
contact-induced fluorescence quenching
Erscheinungsjahr
2005
Zeitschriftentitel
CHEMPHYSCHEM
Band
6
Ausgabe
11
Seite(n)
2277-2285
ISSN
1439-4235
eISSN
1439-7641
Page URI
https://pub.uni-bielefeld.de/record/1601416
Zitieren
Doose S, Neuweiler H, Sauer M. A close look at fluorescence quenching of organic dyes by tryptophan. CHEMPHYSCHEM. 2005;6(11):2277-2285.
Doose, S., Neuweiler, H., & Sauer, M. (2005). A close look at fluorescence quenching of organic dyes by tryptophan. CHEMPHYSCHEM, 6(11), 2277-2285. https://doi.org/10.1002/cphc.200500191
Doose, S, Neuweiler, H, and Sauer, Markus. 2005. “A close look at fluorescence quenching of organic dyes by tryptophan”. CHEMPHYSCHEM 6 (11): 2277-2285.
Doose, S., Neuweiler, H., and Sauer, M. (2005). A close look at fluorescence quenching of organic dyes by tryptophan. CHEMPHYSCHEM 6, 2277-2285.
Doose, S., Neuweiler, H., & Sauer, M., 2005. A close look at fluorescence quenching of organic dyes by tryptophan. CHEMPHYSCHEM, 6(11), p 2277-2285.
S. Doose, H. Neuweiler, and M. Sauer, “A close look at fluorescence quenching of organic dyes by tryptophan”, CHEMPHYSCHEM, vol. 6, 2005, pp. 2277-2285.
Doose, S., Neuweiler, H., Sauer, M.: A close look at fluorescence quenching of organic dyes by tryptophan. CHEMPHYSCHEM. 6, 2277-2285 (2005).
Doose, S, Neuweiler, H, and Sauer, Markus. “A close look at fluorescence quenching of organic dyes by tryptophan”. CHEMPHYSCHEM 6.11 (2005): 2277-2285.
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64 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Zwitterionic near-infrared fluorophore-conjugated epidermal growth factor for fast, real-time, and target-cell-specific cancer imaging.
Kim H, Cho MH, Choi HS, Lee BI, Choi Y., Theranostics 9(4), 2019
PMID: 30867817
Kim H, Cho MH, Choi HS, Lee BI, Choi Y., Theranostics 9(4), 2019
PMID: 30867817
Microsecond sub-domain motions and the folding and misfolding of the mouse prion protein.
Goluguri RR, Sen S, Udgaonkar J., Elife 8(), 2019
PMID: 31025940
Goluguri RR, Sen S, Udgaonkar J., Elife 8(), 2019
PMID: 31025940
Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy.
Beliu G, Kurz AJ, Kuhlemann AC, Behringer-Pliess L, Meub M, Wolf N, Seibel J, Shi ZD, Schnermann M, Grimm JB, Lavis LD, Doose S, Sauer M., Commun Biol 2(), 2019
PMID: 31341960
Beliu G, Kurz AJ, Kuhlemann AC, Behringer-Pliess L, Meub M, Wolf N, Seibel J, Shi ZD, Schnermann M, Grimm JB, Lavis LD, Doose S, Sauer M., Commun Biol 2(), 2019
PMID: 31341960
Optical Sensing of Aromatic Amino Acids and Dipeptides by a Crown-Ether-Functionalized Perylene Bisimide Fluorophore.
Weißenstein A, Saha-Möller CR, Würthner F., Chemistry 24(31), 2018
PMID: 29575389
Weißenstein A, Saha-Möller CR, Würthner F., Chemistry 24(31), 2018
PMID: 29575389
Lipid Structure and Composition Control Consequences of Interleaflet Coupling in Asymmetric Vesicles.
Wang Q, London E., Biophys J 115(4), 2018
PMID: 30082033
Wang Q, London E., Biophys J 115(4), 2018
PMID: 30082033
Structural insights into binding specificity, efficacy and bias of a β2AR partial agonist.
Masureel M, Zou Y, Picard LP, van der Westhuizen E, Mahoney JP, Rodrigues JPGLM, Mildorf TJ, Dror RO, Shaw DE, Bouvier M, Pardon E, Steyaert J, Sunahara RK, Weis WI, Zhang C, Kobilka BK., Nat Chem Biol 14(11), 2018
PMID: 30327561
Masureel M, Zou Y, Picard LP, van der Westhuizen E, Mahoney JP, Rodrigues JPGLM, Mildorf TJ, Dror RO, Shaw DE, Bouvier M, Pardon E, Steyaert J, Sunahara RK, Weis WI, Zhang C, Kobilka BK., Nat Chem Biol 14(11), 2018
PMID: 30327561
Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances.
Pantazis A, Westerberg K, Althoff T, Abramson J, Olcese R., Nat Commun 9(1), 2018
PMID: 30413716
Pantazis A, Westerberg K, Althoff T, Abramson J, Olcese R., Nat Commun 9(1), 2018
PMID: 30413716
Molecular Determinants of BK Channel Functional Diversity and Functioning.
Latorre R, Castillo K, Carrasquel-Ursulaez W, Sepulveda RV, Gonzalez-Nilo F, Gonzalez C, Alvarez O., Physiol Rev 97(1), 2017
PMID: 27807200
Latorre R, Castillo K, Carrasquel-Ursulaez W, Sepulveda RV, Gonzalez-Nilo F, Gonzalez C, Alvarez O., Physiol Rev 97(1), 2017
PMID: 27807200
Integrated view of internal friction in unfolded proteins from single-molecule FRET, contact quenching, theory, and simulations.
Soranno A, Holla A, Dingfelder F, Nettels D, Makarov DE, Schuler B., Proc Natl Acad Sci U S A 114(10), 2017
PMID: 28223518
Soranno A, Holla A, Dingfelder F, Nettels D, Makarov DE, Schuler B., Proc Natl Acad Sci U S A 114(10), 2017
PMID: 28223518
Molecule Counts in Localization Microscopy with Organic Fluorophores.
Karathanasis C, Fricke F, Hummer G, Heilemann M., Chemphyschem 18(8), 2017
PMID: 28196307
Karathanasis C, Fricke F, Hummer G, Heilemann M., Chemphyschem 18(8), 2017
PMID: 28196307
Activation pH and Gating Dynamics of Influenza A M2 Proton Channel Revealed by Single-Molecule Spectroscopy.
Lin CW, Mensa B, Barniol-Xicota M, DeGrado WF, Gai F., Angew Chem Int Ed Engl 56(19), 2017
PMID: 28374543
Lin CW, Mensa B, Barniol-Xicota M, DeGrado WF, Gai F., Angew Chem Int Ed Engl 56(19), 2017
PMID: 28374543
Interaction between triethanolamine and singlet or triplet excited state of xanthene dyes in aqueous solution.
Li S, Zhang H, Lu R, Yu A., Spectrochim Acta A Mol Biomol Spectrosc 184(), 2017
PMID: 28499174
Li S, Zhang H, Lu R, Yu A., Spectrochim Acta A Mol Biomol Spectrosc 184(), 2017
PMID: 28499174
The interaction of amino acids with macrocyclic pH probes of pseudopeptidic nature.
Izquierdo MA, Wadhavane PD, Vigara L, Burguete MI, Galindo F, Luis SV., Photochem Photobiol Sci 16(8), 2017
PMID: 28726954
Izquierdo MA, Wadhavane PD, Vigara L, Burguete MI, Galindo F, Luis SV., Photochem Photobiol Sci 16(8), 2017
PMID: 28726954
Combining short- and long-range fluorescence reporters with simulations to explore the intramolecular dynamics of an intrinsically disordered protein.
Zosel F, Haenni D, Soranno A, Nettels D, Schuler B., J Chem Phys 147(15), 2017
PMID: 29055320
Zosel F, Haenni D, Soranno A, Nettels D, Schuler B., J Chem Phys 147(15), 2017
PMID: 29055320
Placing molecules with Bohr radius resolution using DNA origami.
Funke JJ, Dietz H., Nat Nanotechnol 11(1), 2016
PMID: 26479026
Funke JJ, Dietz H., Nat Nanotechnol 11(1), 2016
PMID: 26479026
Diazaoxatriangulenium: synthesis of reactive derivatives and conjugation to bovine serum albumin.
Bora I, Bogh SA, Rosenberg M, Santella M, Sørensen TJ, Laursen BW., Org Biomol Chem 14(3), 2016
PMID: 26633730
Bora I, Bogh SA, Rosenberg M, Santella M, Sørensen TJ, Laursen BW., Org Biomol Chem 14(3), 2016
PMID: 26633730
Phosphorylation regulates proteolytic efficiency of TEV protease detected by a 5(6)-carboxyfluorescein-pyrene based fluorescent sensor.
He YH, Li YM, Chen YX., Talanta 150(), 2016
PMID: 26838417
He YH, Li YM, Chen YX., Talanta 150(), 2016
PMID: 26838417
Molecular motions that shape the cardiac action potential: Insights from voltage clamp fluorometry.
Zhu W, Varga Z, Silva JR., Prog Biophys Mol Biol 120(1-3), 2016
PMID: 26724572
Zhu W, Varga Z, Silva JR., Prog Biophys Mol Biol 120(1-3), 2016
PMID: 26724572
A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization.
van der Velde JH, Oelerich J, Huang J, Smit JH, Aminian Jazi A, Galiani S, Kolmakov K, Guoridis G, Eggeling C, Herrmann A, Roelfes G, Cordes T., Nat Commun 7(), 2016
PMID: 26751640
van der Velde JH, Oelerich J, Huang J, Smit JH, Aminian Jazi A, Galiani S, Kolmakov K, Guoridis G, Eggeling C, Herrmann A, Roelfes G, Cordes T., Nat Commun 7(), 2016
PMID: 26751640
Multi-target spectrally resolved fluorescence lifetime imaging microscopy.
Niehörster T, Löschberger A, Gregor I, Krämer B, Rahn HJ, Patting M, Koberling F, Enderlein J, Sauer M., Nat Methods 13(3), 2016
PMID: 26808668
Niehörster T, Löschberger A, Gregor I, Krämer B, Rahn HJ, Patting M, Koberling F, Enderlein J, Sauer M., Nat Methods 13(3), 2016
PMID: 26808668
Comparison of Silicon Nanocrystals Prepared by Two Fundamentally Different Methods.
Cibulka O, Vorkötter C, Purkrt A, Holovský J, Benedikt J, Herynková K., Nanoscale Res Lett 11(1), 2016
PMID: 27699716
Cibulka O, Vorkötter C, Purkrt A, Holovský J, Benedikt J, Herynková K., Nanoscale Res Lett 11(1), 2016
PMID: 27699716
Mg2+-dependent conformational changes and product release during DNA-catalyzed RNA ligation monitored by Bimane fluorescence.
Turriani E, Höbartner C, Jovin TM., Nucleic Acids Res 43(1), 2015
PMID: 25505142
Turriani E, Höbartner C, Jovin TM., Nucleic Acids Res 43(1), 2015
PMID: 25505142
Quantitative interpretation of FRET experiments via molecular simulation: force field and validation.
Best RB, Hofmann H, Nettels D, Schuler B., Biophys J 108(11), 2015
PMID: 26039173
Best RB, Hofmann H, Nettels D, Schuler B., Biophys J 108(11), 2015
PMID: 26039173
Photophysical processes in single molecule organic fluorescent probes.
Stennett EM, Ciuba MA, Levitus M., Chem Soc Rev 43(4), 2014
PMID: 24141280
Stennett EM, Ciuba MA, Levitus M., Chem Soc Rev 43(4), 2014
PMID: 24141280
Single-molecule super-resolution imaging by tryptophan-quenching-induced photoswitching of phalloidin-fluorophore conjugates.
Nanguneri S, Flottmann B, Herrmannsdörfer F, Thomas K, Heilemann M., Microsc Res Tech 77(7), 2014
PMID: 24595992
Nanguneri S, Flottmann B, Herrmannsdörfer F, Thomas K, Heilemann M., Microsc Res Tech 77(7), 2014
PMID: 24595992
Recognition of free tryptophan in water by synthetic pseudopeptides: fluorescence and thermodynamic studies.
Martí-Centelles V, Izquierdo MA, Burguete MI, Galindo F, Luis SV., Chemistry 20(24), 2014
PMID: 24788579
Martí-Centelles V, Izquierdo MA, Burguete MI, Galindo F, Luis SV., Chemistry 20(24), 2014
PMID: 24788579
Studies of protein folding and dynamics using single molecule fluorescence spectroscopy.
Basak S, Chattopadhyay K., Phys Chem Chem Phys 16(23), 2014
PMID: 24805942
Basak S, Chattopadhyay K., Phys Chem Chem Phys 16(23), 2014
PMID: 24805942
Fluorescence quenching of (dimethylamino)naphthalene dyes Badan and Prodan by tryptophan in cytochromes P450 and micelles.
Pospíšil P, Luxem KE, Ener M, Sýkora J, Kocábová J, Gray HB, Vlček A, Hof M., J Phys Chem B 118(34), 2014
PMID: 25079965
Pospíšil P, Luxem KE, Ener M, Sýkora J, Kocábová J, Gray HB, Vlček A, Hof M., J Phys Chem B 118(34), 2014
PMID: 25079965
Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy.
Leder V, Lummer M, Tegeler K, Humpert F, Lewinski M, Schüttpelz M, Staiger D., Biochem Biophys Res Commun 453(1), 2014
PMID: 25251471
Leder V, Lummer M, Tegeler K, Humpert F, Lewinski M, Schüttpelz M, Staiger D., Biochem Biophys Res Commun 453(1), 2014
PMID: 25251471
Distance mapping in proteins using fluorescence spectroscopy: tyrosine, like tryptophan, quenches bimane fluorescence in a distance-dependent manner.
Jones Brunette AM, Farrens DL., Biochemistry 53(40), 2014
PMID: 25144569
Jones Brunette AM, Farrens DL., Biochemistry 53(40), 2014
PMID: 25144569
High-throughput development of a hybrid-type fluorescent glutamate sensor for analysis of synaptic transmission.
Takikawa K, Asanuma D, Namiki S, Sakamoto H, Ariyoshi T, Kimpara N, Hirose K., Angew Chem Int Ed Engl 53(49), 2014
PMID: 25297726
Takikawa K, Asanuma D, Namiki S, Sakamoto H, Ariyoshi T, Kimpara N, Hirose K., Angew Chem Int Ed Engl 53(49), 2014
PMID: 25297726
The pH probe CypHer™5E is effectively quenched by FM dyes.
Welzel O, Loy K, Tischbirek CH, Tabor A, Gmeiner P, Kornhuber J, Groemer TW., J Fluoresc 23(3), 2013
PMID: 23397486
Welzel O, Loy K, Tischbirek CH, Tabor A, Gmeiner P, Kornhuber J, Groemer TW., J Fluoresc 23(3), 2013
PMID: 23397486
Long-lived bright red emitting azaoxa-triangulenium fluorophores.
Maliwal BP, Fudala R, Raut S, Kokate R, Sørensen TJ, Laursen BW, Gryczynski Z, Gryczynski I., PLoS One 8(5), 2013
PMID: 23667570
Maliwal BP, Fudala R, Raut S, Kokate R, Sørensen TJ, Laursen BW, Gryczynski Z, Gryczynski I., PLoS One 8(5), 2013
PMID: 23667570
Systematic evaluation of fluorescence correlation spectroscopy data analysis on the nanosecond time scale.
Steger K, Bollmann S, Noé F, Doose S., Phys Chem Chem Phys 15(25), 2013
PMID: 23685745
Steger K, Bollmann S, Noé F, Doose S., Phys Chem Chem Phys 15(25), 2013
PMID: 23685745
Conformational transitions during FtsK translocase activation of individual XerCD-dif recombination complexes.
Zawadzki P, May PF, Baker RA, Pinkney JN, Kapanidis AN, Sherratt DJ, Arciszewska LK., Proc Natl Acad Sci U S A 110(43), 2013
PMID: 24101525
Zawadzki P, May PF, Baker RA, Pinkney JN, Kapanidis AN, Sherratt DJ, Arciszewska LK., Proc Natl Acad Sci U S A 110(43), 2013
PMID: 24101525
Spectroscopic studies of protein folding: linear and nonlinear methods.
Serrano AL, Waegele MM, Gai F., Protein Sci 21(2), 2012
PMID: 22109973
Serrano AL, Waegele MM, Gai F., Protein Sci 21(2), 2012
PMID: 22109973
Relative transmembrane segment rearrangements during BK channel activation resolved by structurally assigned fluorophore-quencher pairing.
Pantazis A, Olcese R., J Gen Physiol 140(2), 2012
PMID: 22802360
Pantazis A, Olcese R., J Gen Physiol 140(2), 2012
PMID: 22802360
Interaction of Escherichia coli RNA polymerase σ70 subunit with promoter elements in the context of free σ70, RNA polymerase holoenzyme, and the β'-σ70 complex.
Mekler V, Pavlova O, Severinov K., J Biol Chem 286(1), 2011
PMID: 20952386
Mekler V, Pavlova O, Severinov K., J Biol Chem 286(1), 2011
PMID: 20952386
Fluorescence correlation spectroscopy of fast chain dynamics within denatured protein L.
Sherman E, Haran G., Chemphyschem 12(3), 2011
PMID: 21271633
Sherman E, Haran G., Chemphyschem 12(3), 2011
PMID: 21271633
Photoinduced electron transfer and fluorophore motion as a probe of the conformational dynamics of membrane proteins: application to the influenza a M2 proton channel.
Rogers JM, Polishchuk AL, Guo L, Wang J, DeGrado WF, Gai F., Langmuir 27(7), 2011
PMID: 21401044
Rogers JM, Polishchuk AL, Guo L, Wang J, DeGrado WF, Gai F., Langmuir 27(7), 2011
PMID: 21401044
Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions.
Bollmann S, Löllmann M, Sauer M, Doose S., Phys Chem Chem Phys 13(28), 2011
PMID: 21687885
Bollmann S, Löllmann M, Sauer M, Doose S., Phys Chem Chem Phys 13(28), 2011
PMID: 21687885
Conformational flexibility of glycosylated peptides.
Bollmann S, Burgert A, Plattner C, Nagel L, Sewald N, Löllmann M, Sauer M, Doose S., Chemphyschem 12(16), 2011
PMID: 21922630
Bollmann S, Burgert A, Plattner C, Nagel L, Sewald N, Löllmann M, Sauer M, Doose S., Chemphyschem 12(16), 2011
PMID: 21922630
Galectin-1-asialofetuin interaction is inhibited by peptides containing the tyr-xxx-tyr motif acting on the glycoprotein.
Wéber E, Hetényi A, Váczi B, Szolnoki E, Fajka-Boja R, Tubak V, Monostori E, Martinek TA., Chembiochem 11(2), 2010
PMID: 19938027
Wéber E, Hetényi A, Váczi B, Szolnoki E, Fajka-Boja R, Tubak V, Monostori E, Martinek TA., Chembiochem 11(2), 2010
PMID: 19938027
Hydrogen-bond driven loop-closure kinetics in unfolded polypeptide chains.
Daidone I, Neuweiler H, Doose S, Sauer M, Smith JC., PLoS Comput Biol 6(1), 2010
PMID: 20098498
Daidone I, Neuweiler H, Doose S, Sauer M, Smith JC., PLoS Comput Biol 6(1), 2010
PMID: 20098498
Light-mediated spatial control via photolabile fluorescently quenched peptide cassettes.
Lee HM, Priestman MA, Lawrence DS., J Am Chem Soc 132(5), 2010
PMID: 20073458
Lee HM, Priestman MA, Lawrence DS., J Am Chem Soc 132(5), 2010
PMID: 20073458
Simultaneous fluorescent monitoring of proteasomal subunit catalysis.
Wakata A, Lee HM, Rommel P, Toutchkine A, Schmidt M, Lawrence DS., J Am Chem Soc 132(5), 2010
PMID: 20078037
Wakata A, Lee HM, Rommel P, Toutchkine A, Schmidt M, Lawrence DS., J Am Chem Soc 132(5), 2010
PMID: 20078037
Suborganelle sensing of mitochondrial cAMP-dependent protein kinase activity.
Agnes RS, Jernigan F, Shell JR, Sharma V, Lawrence DS., J Am Chem Soc 132(17), 2010
PMID: 20380406
Agnes RS, Jernigan F, Shell JR, Sharma V, Lawrence DS., J Am Chem Soc 132(17), 2010
PMID: 20380406
Modulation of intramolecular heterodimer-induced fluorescence quenching of tricarbocyanine dye for the development of fluorescent sensor.
Hirano T, Akiyama J, Mori S, Kagechika H., Org Biomol Chem 8(24), 2010
PMID: 20931144
Hirano T, Akiyama J, Mori S, Kagechika H., Org Biomol Chem 8(24), 2010
PMID: 20931144
Distance mapping in proteins using fluorescence spectroscopy: the tryptophan-induced quenching (TrIQ) method.
Mansoor SE, Dewitt MA, Farrens DL., Biochemistry 49(45), 2010
PMID: 20886836
Mansoor SE, Dewitt MA, Farrens DL., Biochemistry 49(45), 2010
PMID: 20886836
Beyond molecular beacons: optical sensors based on the binding-induced folding of proteins and polypeptides.
Oh KJ, Cash KJ, Plaxco KW., Chemistry 15(10), 2009
PMID: 19191230
Oh KJ, Cash KJ, Plaxco KW., Chemistry 15(10), 2009
PMID: 19191230
Multicolor photoswitching microscopy for subdiffraction-resolution fluorescence imaging.
van de Linde S, Endesfelder U, Mukherjee A, Schüttpelz M, Wiebusch G, Wolter S, Heilemann M, Sauer M., Photochem Photobiol Sci 8(4), 2009
PMID: 19337659
van de Linde S, Endesfelder U, Mukherjee A, Schüttpelz M, Wiebusch G, Wolter S, Heilemann M, Sauer M., Photochem Photobiol Sci 8(4), 2009
PMID: 19337659
Observing proteins as single molecules encapsulated in surface-tethered polymeric nanocontainers.
Rosenkranz T, Katranidis A, Atta D, Gregor I, Enderlein J, Grzelakowski M, Rigler P, Meier W, Fitter J., Chembiochem 10(4), 2009
PMID: 19191249
Rosenkranz T, Katranidis A, Atta D, Gregor I, Enderlein J, Grzelakowski M, Rigler P, Meier W, Fitter J., Chembiochem 10(4), 2009
PMID: 19191249
Position-specific incorporation of fluorescent non-natural amino acids into maltose-binding protein for detection of ligand binding by FRET and fluorescence quenching.
Iijima I, Hohsaka T., Chembiochem 10(6), 2009
PMID: 19301314
Iijima I, Hohsaka T., Chembiochem 10(6), 2009
PMID: 19301314
Quantifying adsorbed protein on surfaces using confocal fluorescence microscopy.
Togashi DM, Ryder AG, Heiss G., Colloids Surf B Biointerfaces 72(2), 2009
PMID: 19423302
Togashi DM, Ryder AG, Heiss G., Colloids Surf B Biointerfaces 72(2), 2009
PMID: 19423302
Fluorescence quenching by photoinduced electron transfer: a reporter for conformational dynamics of macromolecules.
Doose S, Neuweiler H, Sauer M., Chemphyschem 10(9-10), 2009
PMID: 19475638
Doose S, Neuweiler H, Sauer M., Chemphyschem 10(9-10), 2009
PMID: 19475638
Structural effects of biologically relevant rhodamines on spectroscopy of fluorescence fluctuations.
Ferreira JA., Ann N Y Acad Sci 1130(), 2008
PMID: 18596336
Ferreira JA., Ann N Y Acad Sci 1130(), 2008
PMID: 18596336
Confocal laser scanning microscopy as an analytical tool in chromatographic research.
Hubbuch J, Kula MR., Bioprocess Biosyst Eng 31(3), 2008
PMID: 18196281
Hubbuch J, Kula MR., Bioprocess Biosyst Eng 31(3), 2008
PMID: 18196281
Nonequilibrium single molecule protein folding in a coaxial mixer.
Hamadani KM, Weiss S., Biophys J 95(1), 2008
PMID: 18339751
Hamadani KM, Weiss S., Biophys J 95(1), 2008
PMID: 18339751
Mechanism of interactions of alpha-naphthoflavone with cytochrome P450 3A4 explored with an engineered enzyme bearing a fluorescent probe.
Tsalkova TN, Davydova NY, Halpert JR, Davydov DR., Biochemistry 46(1), 2007
PMID: 17198380
Tsalkova TN, Davydova NY, Halpert JR, Davydov DR., Biochemistry 46(1), 2007
PMID: 17198380
Seeing is believing: peptide-based fluorescent sensors of protein tyrosine kinase activity.
Lawrence DS, Wang Q., Chembiochem 8(4), 2007
PMID: 17243187
Lawrence DS, Wang Q., Chembiochem 8(4), 2007
PMID: 17243187
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Doose S, Neuweiler H, Barsch H, Sauer M., Proc Natl Acad Sci U S A 104(44), 2007
PMID: 17956989
Doose S, Neuweiler H, Barsch H, Sauer M., Proc Natl Acad Sci U S A 104(44), 2007
PMID: 17956989
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Kim J, Doose S, Neuweiler H, Sauer M., Nucleic Acids Res 34(9), 2006
PMID: 16687657
Kim J, Doose S, Neuweiler H, Sauer M., Nucleic Acids Res 34(9), 2006
PMID: 16687657
Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry.
Fletcher KA, Fakayode SO, Lowry M, Tucker SA, Neal SL, Kimaru IW, McCarroll ME, Patonay G, Oldham PB, Rusin O, Strongin RM, Warner IM., Anal Chem 78(12), 2006
PMID: 16771540
Fletcher KA, Fakayode SO, Lowry M, Tucker SA, Neal SL, Kimaru IW, McCarroll ME, Patonay G, Oldham PB, Rusin O, Strongin RM, Warner IM., Anal Chem 78(12), 2006
PMID: 16771540
A microscopic view of miniprotein folding: enhanced folding efficiency through formation of an intermediate.
Neuweiler H, Doose S, Sauer M., Proc Natl Acad Sci U S A 102(46), 2005
PMID: 16269542
Neuweiler H, Doose S, Sauer M., Proc Natl Acad Sci U S A 102(46), 2005
PMID: 16269542
52 References
Daten bereitgestellt von Europe PubMed Central.
Seidel, J. Phys. Chem. 100(), 1996
New fluorescent dyes in the red region for biodiagnostics.
Sauer M, Han KT, Muller R, Nord S, Schulz A, Seeger S, Wolfrum J, Arden-Jacob J, Deltau G, Marx NJ, Zander C, Drexhage KH., J Fluoresc 5(3), 1995
PMID: 24226792
Sauer M, Han KT, Muller R, Nord S, Schulz A, Seeger S, Wolfrum J, Arden-Jacob J, Deltau G, Marx NJ, Zander C, Drexhage KH., J Fluoresc 5(3), 1995
PMID: 24226792
Conformational transitions monitored for single molecules in solution.
Edman L, Mets U, Rigler R., Proc. Natl. Acad. Sci. U.S.A. 93(13), 1996
PMID: 8692883
Edman L, Mets U, Rigler R., Proc. Natl. Acad. Sci. U.S.A. 93(13), 1996
PMID: 8692883
Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy.
Eggeling C, Fries JR, Brand L, Gunther R, Seidel CA., Proc. Natl. Acad. Sci. U.S.A. 95(4), 1998
PMID: 9465054
Eggeling C, Fries JR, Brand L, Gunther R, Seidel CA., Proc. Natl. Acad. Sci. U.S.A. 95(4), 1998
PMID: 9465054
Probes for detection of specific DNA sequences at the single-molecule level.
Knemeyer JP, Marme N, Sauer M., Anal. Chem. 72(16), 2000
PMID: 10959954
Knemeyer JP, Marme N, Sauer M., Anal. Chem. 72(16), 2000
PMID: 10959954
Piestert, Nano Lett. 3(), 2003
Heinlein, J. Phys. Chem. B 107(), 2003
Neuweiler, Angew. Chem. 2002, 114 (), 0
AUTHOR UNKNOWN, Angew. Chem. Int. Ed. 41(), 2002
Measurement of submicrosecond intramolecular contact formation in peptides at the single-molecule level.
Neuweiler H, Schulz A, Bohmer M, Enderlein J, Sauer M., J. Am. Chem. Soc. 125(18), 2003
PMID: 12720444
Neuweiler H, Schulz A, Bohmer M, Enderlein J, Sauer M., J. Am. Chem. Soc. 125(18), 2003
PMID: 12720444
Fluorescence quenching of dyes by tryptophan: interactions at atomic detail from combination of experiment and computer simulation.
Vaiana AC, Neuweiler H, Schulz A, Wolfrum J, Sauer M, Smith JC., J. Am. Chem. Soc. 125(47), 2003
PMID: 14624606
Vaiana AC, Neuweiler H, Schulz A, Wolfrum J, Sauer M, Smith JC., J. Am. Chem. Soc. 125(47), 2003
PMID: 14624606
Inter- and intramolecular fluorescence quenching of organic dyes by tryptophan.
Marme N, Knemeyer JP, Sauer M, Wolfrum J., Bioconjug. Chem. 14(6), 2003
PMID: 14624626
Marme N, Knemeyer JP, Sauer M, Wolfrum J., Bioconjug. Chem. 14(6), 2003
PMID: 14624626
Marme, Angew. Chem. 116(), 2004
AUTHOR UNKNOWN, Angew. Chem. Int. Ed. 43(), 2004
Mechanism of quenching of fluorescein by anti-fluorescein IgG antibodies.
Watt RM, Voss EW Jr., Immunochemistry 14(7), 1977
PMID: 303233
Watt RM, Voss EW Jr., Immunochemistry 14(7), 1977
PMID: 303233
Imaging of endosome fusion in BHK fibroblasts based on a novel fluorimetric avidin-biotin binding assay.
Emans N, Biwersi J, Verkman AS., Biophys. J. 69(2), 1995
PMID: 8527685
Emans N, Biwersi J, Verkman AS., Biophys. J. 69(2), 1995
PMID: 8527685
Nazarenko, Nucleic Acids Res. 30(), 2002
Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes.
Nazarenko I, Pires R, Lowe B, Obaidy M, Rashtchian A., Nucleic Acids Res. 30(9), 2002
PMID: 11972350
Nazarenko I, Pires R, Lowe B, Obaidy M, Rashtchian A., Nucleic Acids Res. 30(9), 2002
PMID: 11972350
Karolin, J. Am. Chem. Soc. 116(), 1994
Kurata, Nucleic Acids Res. 29(), 2001
Sauer, Angew. Chem. 115(), 2003
AUTHOR UNKNOWN, Angew. Chem. Int. Ed. 42(), 2003
Dynamics of photoinduced charge transfer and hole transport in synthetic DNA hairpins.
Lewis FD, Letsinger RL, Wasielewski MR., Acc. Chem. Res. 34(2), 2001
PMID: 11263874
Lewis FD, Letsinger RL, Wasielewski MR., Acc. Chem. Res. 34(2), 2001
PMID: 11263874
Wagenknecht, J. Am. Chem. Soc. 122(), 2000
Femtosecond dynamics of flavoproteins: charge separation and recombination in riboflavine (vitamin B2)-binding protein and in glucose oxidase enzyme.
Zhong D, Zewail AH., Proc. Natl. Acad. Sci. U.S.A. 98(21), 2001
PMID: 11592997
Zhong D, Zewail AH., Proc. Natl. Acad. Sci. U.S.A. 98(21), 2001
PMID: 11592997
Protein conformational dynamics probed by single-molecule electron transfer.
Yang H, Luo G, Karnchanaphanurach P, Louie TM, Rech I, Cova S, Xun L, Xie XS., Science 302(5643), 2003
PMID: 14551431
Yang H, Luo G, Karnchanaphanurach P, Louie TM, Rech I, Cova S, Xun L, Xie XS., Science 302(5643), 2003
PMID: 14551431
Using photoinduced charge transfer reactions to study conformational dynamics of biopolymers at the single-molecule level.
Neuweiler H, Sauer M., Curr Pharm Biotechnol 5(3), 2004
PMID: 15180550
Neuweiler H, Sauer M., Curr Pharm Biotechnol 5(3), 2004
PMID: 15180550
Neuweiler, Anal. Chem. 77(), 2005
Fluorescence correlation spectroscopy. II. An experimental realization.
Magde D, Elson EL, Webb WW., Biopolymers 13(1), 1974
PMID: 4818131
Magde D, Elson EL, Webb WW., Biopolymers 13(1), 1974
PMID: 4818131
Rigler, Eur. Biophys. J. 22(), 1993
Biological and chemical applications of fluorescence correlation spectroscopy: a review.
Hess ST, Huang S, Heikal AA, Webb WW., Biochemistry 41(3), 2002
PMID: 11790090
Hess ST, Huang S, Heikal AA, Webb WW., Biochemistry 41(3), 2002
PMID: 11790090
Krichevsky, Rep. Prog. Phys. 65(), 2002
Widengren, Chem. Phys. 216(), 1997
Widengren, J. Phys. Chem. 99(), 1995
Widengren, J. Phys. Chem. A 104(), 2000
Water-soluble quantum dots for multiphoton fluorescence imaging in vivo.
Larson DR, Zipfel WR, Williams RM, Clark SW, Bruchez MP, Wise FW, Webb WW., Science 300(5624), 2003
PMID: 12775841
Larson DR, Zipfel WR, Williams RM, Clark SW, Bruchez MP, Wise FW, Webb WW., Science 300(5624), 2003
PMID: 12775841
Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy.
Doose S, Tsay JM, Pinaud F, Weiss S., Anal. Chem. 77(7), 2005
PMID: 15801758
Doose S, Tsay JM, Pinaud F, Weiss S., Anal. Chem. 77(7), 2005
PMID: 15801758
Lakowicz, 1999
Spectroscopic study and evaluation of red-absorbing fluorescent dyes.
Buschmann V, Weston KD, Sauer M., Bioconjug. Chem. 14(1), 2003
PMID: 12526709
Buschmann V, Weston KD, Sauer M., Bioconjug. Chem. 14(1), 2003
PMID: 12526709
Cohen, J. Chem. Soc. 2(), 1962
Frank, Z. Phys. 69(), 1931
Adams, J. Phys. Chem. B 107(), 2003
Einstein, Ann. Phys. 17(), 1905
AUTHOR UNKNOWN, 0
Sheely, 1932
Viscosity and density of aqueous solutions of urea and guanidine hydrochloride.
Kawahara K, Tanford C., J. Biol. Chem. 241(13), 1966
PMID: 5912116
Kawahara K, Tanford C., J. Biol. Chem. 241(13), 1966
PMID: 5912116
Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy.
Chattopadhyay K, Saffarian S, Elson EL, Frieden C., Biophys. J. 88(2), 2004
PMID: 15556973
Chattopadhyay K, Saffarian S, Elson EL, Frieden C., Biophys. J. 88(2), 2004
PMID: 15556973
Hell, J. Microsc. 169(), 1993
Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules.
Kapanidis AN, Lee NK, Laurence TA, Doose S, Margeat E, Weiss S., Proc. Natl. Acad. Sci. U.S.A. 101(24), 2004
PMID: 15175430
Kapanidis AN, Lee NK, Laurence TA, Doose S, Margeat E, Weiss S., Proc. Natl. Acad. Sci. U.S.A. 101(24), 2004
PMID: 15175430
A fluorescence-based method for direct measurement of submicrosecond intramolecular contact formation in biopolymers: an exploratory study with polypeptides.
Hudgins RR, Huang F, Gramlich G, Nau WM., J. Am. Chem. Soc. 124(4), 2002
PMID: 11804484
Hudgins RR, Huang F, Gramlich G, Nau WM., J. Am. Chem. Soc. 124(4), 2002
PMID: 11804484
Biomolecular and supramolecular kinetics in the submicrosecond time range: the fluorazophore approach.
Nau WM, Wang X., Chemphyschem 3(5), 2002
PMID: 12465497
Nau WM, Wang X., Chemphyschem 3(5), 2002
PMID: 12465497
Determination of ultrafast protein folding rates from loop formation dynamics.
Buscaglia M, Kubelka J, Eaton WA, Hofrichter J., J. Mol. Biol. 347(3), 2005
PMID: 15755457
Buscaglia M, Kubelka J, Eaton WA, Hofrichter J., J. Mol. Biol. 347(3), 2005
PMID: 15755457
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