Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes

Frantescu A, Kakorin S, Tönsing K, Neumann E (2005)
PHYSICAL CHEMISTRY CHEMICAL PHYSICS 7(24): 4126-4131.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The adsorption of calf-thymus DNA-fragments of 300 +/- 50 base pairs (bp) to the outer membrane monolayer of unilamellar lipid vesicles in the presence of Ca2+ ions has been quantified by the standard method of chemical relaxation spectrometry using polarized light. The vesicles of radius a = 150 +/- 45 nm are prepared from bovine brain extract type III containing 80-85% phosphatidylserine (PS) and palmitoyl-oleoyl-phosphatidylcholine (POPC) in the molar ratio PS : 2POPC; total lipid concentration [L-t] = 1 mM in 1 mM HEPES buffer, pH 7.4 at T = 293 K (20 degrees C). The turbidity relaxations of vesicle suspensions, at the wavelength gimel = 365 nm at two characteristic electric field strengths are identified as electroelongation of the whole vesicle coupled to smoothing of thermal membrane undulations and membrane stretching, and at higher fields, to membrane electroporation (MEP). The elongation kinetics indicates that the DNA adsorption renders the membrane more flexible and prone to membrane electroporation (MEP). Remarkably, it is found that the Ca-mediated adsorption of DNA (D) decreases both, bending rigidity K and stretching modulus K, along an unique Langmuir adsorption isotherm for the fraction of bound DNA at the given Ca concentration [Cat] = 0.25 mM. The characteristic chemomechanical parameter of the isotherm is the apparent dissociation equilibrium constant K(D,Ca) = 100 10 mu M (bp) of the ternary complex DCaB of DNA base pairs (bp) and Ca binding to sites B on the outer vesicle surface. Whereas both K and K decrease in the presence of high electric fields (E), the key parameter K(D,Ca) is independent of E in the range 0 <= E/(kV cm(-1)) <= 40.
Erscheinungsjahr
Zeitschriftentitel
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Band
7
Ausgabe
24
Seite(n)
4126-4131
ISSN
eISSN
PUB-ID

Zitieren

Frantescu A, Kakorin S, Tönsing K, Neumann E. Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes. PHYSICAL CHEMISTRY CHEMICAL PHYSICS. 2005;7(24):4126-4131.
Frantescu, A., Kakorin, S., Tönsing, K., & Neumann, E. (2005). Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 7(24), 4126-4131. doi:10.1039/b510882a
Frantescu, A., Kakorin, S., Tönsing, K., and Neumann, E. (2005). Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes. PHYSICAL CHEMISTRY CHEMICAL PHYSICS 7, 4126-4131.
Frantescu, A., et al., 2005. Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 7(24), p 4126-4131.
A. Frantescu, et al., “Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes”, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, vol. 7, 2005, pp. 4126-4131.
Frantescu, A., Kakorin, S., Tönsing, K., Neumann, E.: Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes. PHYSICAL CHEMISTRY CHEMICAL PHYSICS. 7, 4126-4131 (2005).
Frantescu, A, Kakorin, Sergej, Tönsing, Katja, and Neumann, Eberhard. “Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes”. PHYSICAL CHEMISTRY CHEMICAL PHYSICS 7.24 (2005): 4126-4131.

7 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Physical non-viral gene delivery methods for tissue engineering.
Mellott AJ, Forrest ML, Detamore MS., Ann Biomed Eng 41(3), 2013
PMID: 23099792
Gene electrotransfer: from biophysical mechanisms to in vivo applications : Part 1- Biophysical mechanisms.
Escoffre JM, Mauroy C, Portet T, Wasungu L, Rosazza C, Gilbart Y, Mallet L, Bellard E, Golzio M, Rols MP, Teissié J., Biophys Rev 1(4), 2009
PMID: 28510029
Molecular origin of model membrane bending rigidity.
Kurtisovski E, Taulier N, Ober R, Waks M, Urbach W., Phys Rev Lett 98(25), 2007
PMID: 17678060

43 References

Daten bereitgestellt von Europe PubMed Central.

Gene transfer into mouse lyoma cells by electroporation in high electric fields.
Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH., EMBO J. 1(7), 1982
PMID: 6329708
High-efficiency gene transfer into skeletal muscle mediated by electric pulses.
Mir LM, Bureau MF, Gehl J, Rangara R, Rouy D, Caillaud JM, Delaere P, Branellec D, Schwartz B, Scherman D., Proc. Natl. Acad. Sci. U.S.A. 96(8), 1999
PMID: 10200250

Mir, Bioelectrochem. Bioenerg. 38(), 1995
Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis.
Satkauskas S, Bureau MF, Puc M, Mahfoudi A, Scherman D, Miklavcic D, Mir LM., Mol. Ther. 5(2), 2002
PMID: 11829520
Tumor regression by combination antisense therapy against Plk1 and Bcl-2.
Elez R, Piiper A, Kronenberger B, Kock M, Brendel M, Hermann E, Pliquett U, Neumann E, Zeuzem S., Oncogene 22(1), 2003
PMID: 12527909
Mechanism of electroporative dye uptake by mouse B cells.
Neumann E, Toensing K, Kakorin S, Budde P, Frey J., Biophys. J. 74(1), 1998
PMID: 9449314
Annexin V and vesicle membrane electroporation.
Tonsing K, Kakorin S, Neumann E, Liemann S, Huber R., Eur. Biophys. J. 26(4), 1997
PMID: 9378099

Neumann, Radiol. Oncol 32(), 1998

Lipowsky, Europhys. Lett. 43(), 1998

Lipowsky, Europhys. Lett. 30(), 1995

Simon, Chem. Phys. Lipids 76(), 1995

Doebereiner, Eur. Biophys. J. 28(), 1999

Kakorin, Ber. Bunsen-Ges. Phys. Chem. 102(), 1998
Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation by electroporation.
Neumann E, Kakorin S, Tsoneva I, Nikolova B, Tomov T., Biophys. J. 71(2), 1996
PMID: 8842225
Probing DNA-cationic lipid interactions with the fluorophore trimethylammonium diphenyl-hexatriene (TMADPH).
Hirsch-Lerner D, Barenholz Y., Biochim. Biophys. Acta 1370(1), 1998
PMID: 9518536
Biophysical characterization of cationic lipid: DNA complexes.
Eastman SJ, Siegel C, Tousignant J, Smith AE, Cheng SH, Scheule RK., Biochim. Biophys. Acta 1325(1), 1997
PMID: 9106482
Calcium enhances the transfection potency of plasmid DNA-cationic liposome complexes.
Lam AM, Cullis PR., Biochim. Biophys. Acta 1463(2), 2000
PMID: 10675506
The phase behavior of cationic lipid-DNA complexes.
May S, Harries D, Ben-Shaul A., Biophys. J. 78(4), 2000
PMID: 10733951
Comparison of two liposome fusion assays monitoring the intermixing of aqueous contents and of membrane components.
Rosenberg J, Duzgunes N, Kayalar C., Biochim. Biophys. Acta 735(1), 1983
PMID: 6626546

Cherney, Anal. Chem. 78(), 2004

Clement, J. Phys. II 7(), 1997

Skau, Eur. Phys. J. E 7(), 2002

Kakorin, J. Phys. Chem. B 107(), 2003
Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces.
Frantescu A, Tonsing K, Neumann E., Bioelectrochemistry 68(2), 2005
PMID: 16125472

Griese, Phys. Chem. Chem. Phys. 4(), 2002

Kakorin, Ber. Bunsen-Ges. Phys. Chem. 100(), 1996
Conformation changes in rRNA induced by electric impulses.
Revzin A, Neumann E., Biophys. Chem. 2(2), 1974
PMID: 4611518
Cholesterol reduces membrane electroporation and electric deformation of small bilayer vesicles.
Kakorin S, Brinkmann U, Neumann E., Biophys. Chem. 117(2), 2005
PMID: 15923075
Light scattering and turbidity measurements on lipid vesicles.
Chong CS, Colbow K., Biochim. Biophys. Acta 436(2), 1976
PMID: 1276217
Light scattering by a core-mantle spheroidal particle.
Farafonov VG, Voshchinnikov NV, Somsikov VV., Appl Opt 35(27), 1996
PMID: 21127540

Neumann, Faraday Discuss. 111(), 1998

Kakorin, Eur. Biophys. J. 27(), 1998

Kakorin, Ber. Bunsen-Ges. Phys. Chem. 102(), 1998
Electrical properties of tissue and cell suspensions.
SCHWAN HP., Adv Biol Med Phys 5(), 1957
PMID: 13520431
Strength of Ca(2+) binding to retinal lipid membranes: consequences for lipid organization.
Huster D, Arnold K, Gawrisch K., Biophys. J. 78(6), 2000
PMID: 10827979
Water permeability of polyunsaturated lipid membranes measured by 17O NMR.
Huster D, Jin AJ, Arnold K, Gawrisch K., Biophys. J. 73(2), 1997
PMID: 9251802
Phase transition of dimyristoylphosphatidylglycerol bilayers induced by electric field pulses.
Thurk M, Porschke D., Biochim. Biophys. Acta 1067(2), 1991
PMID: 1878369

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 16474877
PubMed | Europe PMC

Suchen in

Google Scholar