Mossbauer studies of Cu(II) ions interaction with the non-heme iron and cytochrome b(559) in a Chlamydomonas reinhardtii PSI minus mutant

Burda K, Kruk J, Strzalka K, Stanek J, Schmid GH, Kruse O (2006)
In: Acta Physica Polonica A. ACTA PHYSICA POLONICA A, 109(3). POLISH ACAD SCIENCES INST PHYSICS: 237-247.

Konferenzbeitrag | Veröffentlicht | Englisch
 
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Autor*in
Burda, K; Kruk, J; Strzalka, K; Stanek, J; Schmid, GH; Kruse, OlafUniBi
Abstract / Bemerkung
Mossbauer spectroscopy was applied, for the first time, to study the interaction of copper ions with the non-heme iron and the heme iron of cytochrome b(559) in photosystem II thylakoids isolated from a Chlamydomonas reinhardtii photosystem I minus mutant. We showed that copper ions oxidize the heme iron and change its low spin state into a high spin state. This is probably due to deprotonation of the histidine coordinating the heme. We also found that copper preserves the non-heme iron in a low spin ferrous state, enhancing the covalence of iron bonds as compared to the untreated sample. We Suggest that a, disruption of hydrogen bonds stabilizing the quinone-iron complex by Cu2+ is the mechanism responsible for a new arrangement of the binding site of the non-heme iron leading to its more "tense" structure. Such a diamagnetic state of the non-heme iron induced by copper results in a magnetic decoupling of iron from the primary quinone acceptor. These results indicate that Cu does not cause removal of the non-heme iron from its binding site. The observed Cu2+ action on the non-heme iron and cytochrome b559 is similar to that previously observed for alpha-tocopherol quinone.
Erscheinungsjahr
2006
Titel des Konferenzbandes
Acta Physica Polonica A
Band
109
Ausgabe
3
Seite(n)
237-247
ISSN
0587-4246
Page URI
https://pub.uni-bielefeld.de/record/1599952

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Burda K, Kruk J, Strzalka K, Stanek J, Schmid GH, Kruse O. Mossbauer studies of Cu(II) ions interaction with the non-heme iron and cytochrome b(559) in a Chlamydomonas reinhardtii PSI minus mutant. In: Acta Physica Polonica A. ACTA PHYSICA POLONICA A. Vol 109. POLISH ACAD SCIENCES INST PHYSICS; 2006: 237-247.
Burda, K., Kruk, J., Strzalka, K., Stanek, J., Schmid, G. H., & Kruse, O. (2006). Mossbauer studies of Cu(II) ions interaction with the non-heme iron and cytochrome b(559) in a Chlamydomonas reinhardtii PSI minus mutant. Acta Physica Polonica A, ACTA PHYSICA POLONICA A, 109, 237-247. POLISH ACAD SCIENCES INST PHYSICS. doi:10.12693/APhysPolA.109.237
Burda, K., Kruk, J., Strzalka, K., Stanek, J., Schmid, G. H., and Kruse, O. (2006). “Mossbauer studies of Cu(II) ions interaction with the non-heme iron and cytochrome b(559) in a Chlamydomonas reinhardtii PSI minus mutant” in Acta Physica Polonica A ACTA PHYSICA POLONICA A, vol. 109, (POLISH ACAD SCIENCES INST PHYSICS), 237-247.
Burda, K., et al., 2006. Mossbauer studies of Cu(II) ions interaction with the non-heme iron and cytochrome b(559) in a Chlamydomonas reinhardtii PSI minus mutant. In Acta Physica Polonica A. ACTA PHYSICA POLONICA A. no.109 POLISH ACAD SCIENCES INST PHYSICS, pp. 237-247.
K. Burda, et al., “Mossbauer studies of Cu(II) ions interaction with the non-heme iron and cytochrome b(559) in a Chlamydomonas reinhardtii PSI minus mutant”, Acta Physica Polonica A, ACTA PHYSICA POLONICA A, vol. 109, POLISH ACAD SCIENCES INST PHYSICS, 2006, pp.237-247.
Burda, K., Kruk, J., Strzalka, K., Stanek, J., Schmid, G.H., Kruse, O.: Mossbauer studies of Cu(II) ions interaction with the non-heme iron and cytochrome b(559) in a Chlamydomonas reinhardtii PSI minus mutant. Acta Physica Polonica A. ACTA PHYSICA POLONICA A. 109, p. 237-247. POLISH ACAD SCIENCES INST PHYSICS (2006).
Burda, K, Kruk, J, Strzalka, K, Stanek, J, Schmid, GH, and Kruse, Olaf. “Mossbauer studies of Cu(II) ions interaction with the non-heme iron and cytochrome b(559) in a Chlamydomonas reinhardtii PSI minus mutant”. Acta Physica Polonica A. POLISH ACAD SCIENCES INST PHYSICS, 2006.Vol. 109. ACTA PHYSICA POLONICA A. 237-247.