Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode
Ataka K, Richter B, Heberle J (2006)
JOURNAL OF PHYSICAL CHEMISTRY B 110(18): 9339-9347.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Ataka, K;
Richter, B;
Heberle, J
Abstract / Bemerkung
The physiological reaction of a membrane protein is reconstituted on a solid-supported electrode by orientational control via the position of an affinity tag. Recombinant cytochrome c oxidase (CcO) from Rhodobacter sphaeroides is immobilized on a chemically modified gold surface via the affinity of a histidine tag ( His-tag) to a nickel chelating nitrilotriacetic acid surface. Control of the orientation is achieved by the adsorption of CcO through the His-tag engineered into the two opposite sites of the membrane protein surface. After reconstitution into a lipid layer, the functionality of this enzyme film electrode is probed by surface-enhanced infrared absorption spectroscopy and cyclic voltammetry. We demonstrate that cytochrome c ( Cc) binds and initiates the catalytic reaction of CcO only when the latter is orientated with subunit II facing the bulk aqueous phase while Cc does not interact with the oppositely orientated CcO. We infer from the observed catalytic dioxygen reduction at potentials below 240 mV ( vs a normal hydrogen electrode) that reduced Cc mediates electron input into CcO in a way similar to the physiological pathway. The quantitative analysis of the IR spectra indicates the presence of an inactive population of Cc bound to CcO at equal amounts as the redox-active population. This methodological approach demonstrates that the orientation of the membrane protein can be controlled depending on the position of the affinity tag. The approach is considered to be of general applicability as the introduction of affinity tags is routine in current biochemistry.
Erscheinungsjahr
2006
Zeitschriftentitel
JOURNAL OF PHYSICAL CHEMISTRY B
Band
110
Ausgabe
18
Seite(n)
9339-9347
ISSN
1520-6106
eISSN
1520-5207
Page URI
https://pub.uni-bielefeld.de/record/1599417
Zitieren
Ataka K, Richter B, Heberle J. Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode. JOURNAL OF PHYSICAL CHEMISTRY B. 2006;110(18):9339-9347.
Ataka, K., Richter, B., & Heberle, J. (2006). Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode. JOURNAL OF PHYSICAL CHEMISTRY B, 110(18), 9339-9347. https://doi.org/10.1021/jp0534131
Ataka, K, Richter, B, and Heberle, J. 2006. “Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode”. JOURNAL OF PHYSICAL CHEMISTRY B 110 (18): 9339-9347.
Ataka, K., Richter, B., and Heberle, J. (2006). Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode. JOURNAL OF PHYSICAL CHEMISTRY B 110, 9339-9347.
Ataka, K., Richter, B., & Heberle, J., 2006. Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode. JOURNAL OF PHYSICAL CHEMISTRY B, 110(18), p 9339-9347.
K. Ataka, B. Richter, and J. Heberle, “Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode”, JOURNAL OF PHYSICAL CHEMISTRY B, vol. 110, 2006, pp. 9339-9347.
Ataka, K., Richter, B., Heberle, J.: Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode. JOURNAL OF PHYSICAL CHEMISTRY B. 110, 9339-9347 (2006).
Ataka, K, Richter, B, and Heberle, J. “Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode”. JOURNAL OF PHYSICAL CHEMISTRY B 110.18 (2006): 9339-9347.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
19 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Mid-infrared spectroscopy for protein analysis: potential and challenges.
López-Lorente ÁI, Mizaikoff B., Anal Bioanal Chem 408(11), 2016
PMID: 26879650
López-Lorente ÁI, Mizaikoff B., Anal Bioanal Chem 408(11), 2016
PMID: 26879650
In-Situ Observation of Membrane Protein Folding during Cell-Free Expression.
Baumann A, Kerruth S, Fitter J, Büldt G, Heberle J, Schlesinger R, Ataka K., PLoS One 11(3), 2016
PMID: 26978519
Baumann A, Kerruth S, Fitter J, Büldt G, Heberle J, Schlesinger R, Ataka K., PLoS One 11(3), 2016
PMID: 26978519
Reconstitution of supramolecular organization involved in energy metabolism at electrochemical interfaces for biosensing and bioenergy production.
Roger M, de Poulpiquet A, Ciaccafava A, Ilbert M, Guiral M, Giudici-Orticoni MT, Lojou E., Anal Bioanal Chem 406(4), 2014
PMID: 24292430
Roger M, de Poulpiquet A, Ciaccafava A, Ilbert M, Guiral M, Giudici-Orticoni MT, Lojou E., Anal Bioanal Chem 406(4), 2014
PMID: 24292430
Surface-enhanced infrared absorption spectroscopy (SEIRAS) to probe monolayers of membrane proteins.
Ataka K, Stripp ST, Heberle J., Biochim Biophys Acta 1828(10), 2013
PMID: 23816441
Ataka K, Stripp ST, Heberle J., Biochim Biophys Acta 1828(10), 2013
PMID: 23816441
Analyzing the catalytic processes of immobilized redox enzymes by vibrational spectroscopies.
Sezer M, Millo D, Weidinger IM, Zebger I, Hildebrandt P., IUBMB Life 64(6), 2012
PMID: 22535701
Sezer M, Millo D, Weidinger IM, Zebger I, Hildebrandt P., IUBMB Life 64(6), 2012
PMID: 22535701
Vibrational spectroscopy of biomembranes.
Schultz ZD, Levin IW., Annu Rev Anal Chem (Palo Alto Calif) 4(), 2011
PMID: 21456972
Schultz ZD, Levin IW., Annu Rev Anal Chem (Palo Alto Calif) 4(), 2011
PMID: 21456972
2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer.
Nowak C, Laredo T, Gebert J, Lipkowski J, Gennis RB, Ferguson-Miller S, Knoll W, Naumann RL., Metallomics 3(6), 2011
PMID: 21541411
Nowak C, Laredo T, Gebert J, Lipkowski J, Gennis RB, Ferguson-Miller S, Knoll W, Naumann RL., Metallomics 3(6), 2011
PMID: 21541411
Scanning near-field IR microscopy of proteins in lipid bilayers.
Ballout F, Krassen H, Kopf I, Ataka K, Bründermann E, Heberle J, Havenith M., Phys Chem Chem Phys 13(48), 2011
PMID: 22048276
Ballout F, Krassen H, Kopf I, Ataka K, Bründermann E, Heberle J, Havenith M., Phys Chem Chem Phys 13(48), 2011
PMID: 22048276
Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry.
Dunn JD, Reid GE, Bruening ML., Mass Spectrom Rev 29(1), 2010
PMID: 19263479
Dunn JD, Reid GE, Bruening ML., Mass Spectrom Rev 29(1), 2010
PMID: 19263479
Field-effect detection using phospholipid membranes.
Kataoka-Hamai C, Miyahara Y., Sci Technol Adv Mater 11(3), 2010
PMID: 27877335
Kataoka-Hamai C, Miyahara Y., Sci Technol Adv Mater 11(3), 2010
PMID: 27877335
Thinner, smaller, faster: IR techniques to probe the functionality of biological and biomimetic systems.
Ataka K, Kottke T, Heberle J., Angew Chem Int Ed Engl 49(32), 2010
PMID: 20818765
Ataka K, Kottke T, Heberle J., Angew Chem Int Ed Engl 49(32), 2010
PMID: 20818765
Monitoring catalysis of the membrane-bound hydrogenase from Ralstonia eutropha H16 by surface-enhanced IR absorption spectroscopy.
Wisitruangsakul N, Lenz O, Ludwig M, Friedrich B, Lendzian F, Hildebrandt P, Zebger I., Angew Chem Int Ed Engl 48(3), 2009
PMID: 19067445
Wisitruangsakul N, Lenz O, Ludwig M, Friedrich B, Lendzian F, Hildebrandt P, Zebger I., Angew Chem Int Ed Engl 48(3), 2009
PMID: 19067445
XPS, TOF-SIMS, NEXAFS, and SPR characterization of nitrilotriacetic acid-terminated self-assembled monolayers for controllable immobilization of proteins.
Cheng F, Gamble LJ, Castner DG., Anal Chem 80(7), 2008
PMID: 18302347
Cheng F, Gamble LJ, Castner DG., Anal Chem 80(7), 2008
PMID: 18302347
In situ monitoring of the catalytic activity of cytochrome C oxidase in a biomimetic architecture.
Friedrich MG, Plum MA, Santonicola MG, Kirste VU, Knoll W, Ludwig B, Naumann RL., Biophys J 95(3), 2008
PMID: 18441024
Friedrich MG, Plum MA, Santonicola MG, Kirste VU, Knoll W, Ludwig B, Naumann RL., Biophys J 95(3), 2008
PMID: 18441024
Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy.
Jiang X, Zaitseva E, Schmidt M, Siebert F, Engelhard M, Schlesinger R, Ataka K, Vogel R, Heberle J., Proc Natl Acad Sci U S A 105(34), 2008
PMID: 18719097
Jiang X, Zaitseva E, Schmidt M, Siebert F, Engelhard M, Schlesinger R, Ataka K, Vogel R, Heberle J., Proc Natl Acad Sci U S A 105(34), 2008
PMID: 18719097
In situ monitoring of the orientated assembly of strep-tagged membrane proteins on the gold surface by surface enhanced infrared absorption spectroscopy.
Jiang X, Zuber A, Heberle J, Ataka K., Phys Chem Chem Phys 10(42), 2008
PMID: 18972026
Jiang X, Zuber A, Heberle J, Ataka K., Phys Chem Chem Phys 10(42), 2008
PMID: 18972026
Modulation of the electrochemical behavior of tyrosyl radicals by the electrode surface.
Machczynski MC, Kuhl KP, McGuirl MA., Anal Biochem 362(1), 2007
PMID: 17254538
Machczynski MC, Kuhl KP, McGuirl MA., Anal Biochem 362(1), 2007
PMID: 17254538
Biochemical applications of surface-enhanced infrared absorption spectroscopy.
Ataka K, Heberle J., Anal Bioanal Chem 388(1), 2007
PMID: 17242890
Ataka K, Heberle J., Anal Bioanal Chem 388(1), 2007
PMID: 17242890
References
Daten bereitgestellt von Europe PubMed Central.
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 16671753
PubMed | Europe PMC
Suchen in