Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces

Frantescu A, Tönsing K, Neumann E (2006)
BIOELECTROCHEMISTRY 68(2): 158-170.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The electroporative transfer of gene DNA and other bioactive substances into tissue cells by electric pulses gains increasing importance in the new disciplines of electrochemotherapy and electrogenetherapy. The efficiency of the electrotransfer depends crucially on the adsorption of the gene DNA and oligonucleotides to the plasma cell membranes. Here it is shown that the adsorption of larger oligonucleotides such as fragments (ca. 300 bp) of sonicated calf-thymus DNA, to anionic lipids of unilamellar vesicles (diameter Phi = 300 +/- 90 nm) is greatly enhanced by divalent cations such as Ca2+-ions. Applying centrifugation, bound and free DNA are monitored optically at the wavelength = 260 nm. Using arsenazo III as a Ca2+-indicator and atomic absorption spectroscopy (AAS), Ca2+-titrations of DNA and vesicles yield the individual equilibrium constants of Ca2+-and DNA-binding not only for the binary complexes: Ca/lipids, Ca/DNA and DNA/lipids, respectively, but also for the various processes to form the ternary complex DNA/Ca/lipids. The data provide the basis for goal-directed optimization protocols for the adsorption and thus efficient electrotransfer of oligonucleotides and polynucleotides into cells. (c) 2005 Elsevier B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
BIOELECTROCHEMISTRY
Band
68
Ausgabe
2
Seite(n)
158-170
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PUB-ID

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Frantescu A, Tönsing K, Neumann E. Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces. BIOELECTROCHEMISTRY. 2006;68(2):158-170.
Frantescu, A., Tönsing, K., & Neumann, E. (2006). Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces. BIOELECTROCHEMISTRY, 68(2), 158-170. doi:10.1016/j.bioelechem.2005.06.003
Frantescu, A., Tönsing, K., and Neumann, E. (2006). Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces. BIOELECTROCHEMISTRY 68, 158-170.
Frantescu, A., Tönsing, K., & Neumann, E., 2006. Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces. BIOELECTROCHEMISTRY, 68(2), p 158-170.
A. Frantescu, K. Tönsing, and E. Neumann, “Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces”, BIOELECTROCHEMISTRY, vol. 68, 2006, pp. 158-170.
Frantescu, A., Tönsing, K., Neumann, E.: Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces. BIOELECTROCHEMISTRY. 68, 158-170 (2006).
Frantescu, A, Tönsing, Katja, and Neumann, Eberhard. “Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces”. BIOELECTROCHEMISTRY 68.2 (2006): 158-170.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Adsorption of DNA onto anionic lipid surfaces.
Martín-Molina A, Luque-Caballero G, Faraudo J, Quesada-Pérez M, Maldonado-Valderrama J., Adv Colloid Interface Sci 206(), 2014
PMID: 24359695
Using AFM to probe the complexation of DNA with anionic lipids mediated by Ca(2+): the role of surface pressure.
Luque-Caballero G, Martín-Molina A, Sánchez-Treviño AY, Rodríguez-Valverde MA, Cabrerizo-Vílchez MA, Maldonado-Valderrama J., Soft Matter 10(16), 2014
PMID: 24668321
Adsorption of DNA and electric fields decrease the rigidity of lipid vesicle membranes.
Frantescu A, Kakorin S, Toensing K, Neumann E., Phys Chem Chem Phys 7(24), 2005
PMID: 16474877

36 References

Daten bereitgestellt von Europe PubMed Central.

High-efficiency gene transfer into skeletal muscle mediated by electric pulses.
Mir LM, Bureau MF, Gehl J, Rangara R, Rouy D, Caillaud JM, Delaere P, Branellec D, Schwartz B, Scherman D., Proc. Natl. Acad. Sci. U.S.A. 96(8), 1999
PMID: 10200250
Gene transfer into mouse lyoma cells by electroporation in high electric fields.
Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH., EMBO J. 1(7), 1982
PMID: 6329708
Electroporation of subcutaneous mouse tumors by rectangular and trapezium high voltage pulses.
Pliquett U, Elez R, Piiper A, Neumann E., Bioelectrochemistry 62(1), 2004
PMID: 14990329
Biomedical applications of electric pulses with special emphasis on antitumor electrochemotherapy
Mir, Bioelectrochem. Bioenerg. 38(), 1995
Tumor regression by combination antisense therapy against Plk1 and Bcl-2.
Elez R, Piiper A, Kronenberger B, Kock M, Brendel M, Hermann E, Pliquett U, Neumann E, Zeuzem S., Oncogene 22(1), 2003
PMID: 12527909
Specificity of Ca2+ and Mg2+ binding to phosphatidylserine vesicles and resultant phase changes of bilayer membrane structure.
Newton C, Pangborn W, Nir S, Papahadjopoulos D., Biochim. Biophys. Acta 506(2), 1978
PMID: 620034
Adsorption of divalent cations to bilayer membranes containing phosphatidylserine.
McLaughlin S, Mulrine N, Gresalfi T, Vaio G, McLaughlin A., J. Gen. Physiol. 77(4), 1981
PMID: 7241089
Calcium enhances the transfection potency of plasmid DNA-cationic liposome complexes.
Lam AM, Cullis PR., Biochim. Biophys. Acta 1463(2), 2000
PMID: 10675506
The binding of small cations to deoxyribonucleic acid. Nucleotide specificity.
Shapiro JT, Stannard BS, Felsenfeld G., Biochemistry 8(8), 1969
PMID: 4897330
Interaction of metallic cations with DNA VI. Specific binding of Mg2+ and Mn2+
Clement, Biopolymers 12(), 1973
A nanoscale polynucleotide-neutral liposome self-assemblies formulated for therapeutic gene delivery
Süleymanoglu, Electron. J. Biomed. 2(2), 2004
Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation by electroporation.
Neumann E, Kakorin S, Tsoneva I, Nikolova B, Tomov T., Biophys. J. 71(2), 1996
PMID: 8842225
Kinetics of calcium-induced fusion of cell-size liposomes with monolayers in solutions of different osmolarity
Stoicheva, Z. Naturforsch. 40c(), 1985
Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes.
Olson F, Hunt CA, Szoka FC, Vail WJ, Papahadjopoulos D., Biochim. Biophys. Acta 557(1), 1979
PMID: 95096
Vesicles of variable sizes produced by a rapid extrusion procedure.
Mayer LD, Hope MJ, Cullis PR., Biochim. Biophys. Acta 858(1), 1986
PMID: 3707960
Measurements of DNA lengths remaining in a viral capsid after osmotically suppressed partial ejection.
Evilevitch A, Gober JW, Phillips M, Knobler CM, Gelbart WM., Biophys. J. 88(1), 2004
PMID: 15489301
Molecular weight and long-range structure
Bloomfield, 1974
Comparison of two liposome fusion assays monitoring the intermixing of aqueous contents and of membrane components.
Rosenberg J, Duzgunes N, Kayalar C., Biochim. Biophys. Acta 735(1), 1983
PMID: 6626546
Winding of the DNA helix by divalent metal ions.
Xu YC, Bremer H., Nucleic Acids Res. 25(20), 1997
PMID: 9321659
Photometric determination of micro amounts of calcium with arsenazo III
Michaylova, Anal. Chim. Acta 53(), 1971
Kinetic scheme for Ca2+-arsenazo III interactions.
Dorogi PL, Rabl CR, Neumann E., Biochem. Biophys. Res. Commun. 111(3), 1983
PMID: 6838582
Determination of calcium and magnesium in urine by atomic absorption spectroscopy
Willis, Anal. Chem. 33(), 1961
Strength of Ca(2+) binding to retinal lipid membranes: consequences for lipid organization.
Huster D, Arnold K, Gawrisch K., Biophys. J. 78(6), 2000
PMID: 10827979
Ionic structure of phospholipid membranes, and binding of calcium ions.
Seimiya T, Ohki S., Biochim. Biophys. Acta 298(3), 1973
PMID: 4736818
Binding of cations to phosphatidylserine vesicles
Nir, Bioelectrochem. Bioenerg. 5(), 1978

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