Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics

Ross J, Buschkamp P, Fetting D, Donnermeyer A, Roth CM, Tinnefeld P (2007)
Journal of Physical Chemistry B 111(2): 321-326.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ;
Abstract / Bemerkung
We have developed confocal multicolor single-molecule spectroscopy with optimized detection sensitivity on three spectrally distinct channels for the study of biomolecular interactions and FRET between more than two molecules. Using programmable acousto-optical devices as beamsplitter and excitation filter, we overcome some of the limitations of conventional multichroic beamsplitters and implement rapid alternation between three laser lines. This enables to visualize the synthesis of DNA three-way junctions on a single-molecule basis and to resolve seven stoichiometric subpopulations as well as to quantify FRET in the presence of competing energy transfer pathways. Furthermore, the ability to study correlated molecular movements by monitoring several distances within a biomolecular complex simultaneously is demonstrated.
Erscheinungsjahr
2007
Zeitschriftentitel
Journal of Physical Chemistry B
Band
111
Ausgabe
2
Seite(n)
321-326
ISSN
1520-6106
Page URI
https://pub.uni-bielefeld.de/record/1596294

Zitieren

Ross J, Buschkamp P, Fetting D, Donnermeyer A, Roth CM, Tinnefeld P. Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B. 2007;111(2):321-326.
Ross, J., Buschkamp, P., Fetting, D., Donnermeyer, A., Roth, C. M., & Tinnefeld, P. (2007). Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B, 111(2), 321-326. doi:10.1021/jp066082g
Ross, J., Buschkamp, P., Fetting, D., Donnermeyer, A., Roth, C. M., and Tinnefeld, P. (2007). Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B 111, 321-326.
Ross, J., et al., 2007. Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B, 111(2), p 321-326.
J. Ross, et al., “Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics”, Journal of Physical Chemistry B, vol. 111, 2007, pp. 321-326.
Ross, J., Buschkamp, P., Fetting, D., Donnermeyer, A., Roth, C.M., Tinnefeld, P.: Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics. Journal of Physical Chemistry B. 111, 321-326 (2007).
Ross, Joachim, Buschkamp, Peter, Fetting, Daniel, Donnermeyer, Achim, Roth, Christian M., and Tinnefeld, Philip. “Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics”. Journal of Physical Chemistry B 111.2 (2007): 321-326.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17214479
PubMed | Europe PMC

Suchen in

Google Scholar