Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches

Colditz F, Niehaus K, Krajinski F (2007)
Planta 226(1): 57-71.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Colditz, Frank; Niehaus, KarstenUniBi; Krajinski, Franziska
Einrichtung
Fakultät für Biologie > Proteom- und Metabolomforschung
Centrum für Biotechnologie > Arbeitsgruppe K. Niehaus
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Abstract / Bemerkung
Recent studies on the root proteome of Medicago truncatula (Gaertn.) showed an induction of pathogenesis-related (PR) proteins of the class 10 after infection with the oomycete pathogen Aphanomyces euteiches (Drechs.). To get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using RNAi was carried out. Agrobacterium rhizogenes-mediated transformation of M. truncatula roots led to a knockdown of the Medicago PR10-1 gene in transgenic in vitro root cultures. Proteomic analyses of the MtPr10-1i root cultures showed that MtPr10-1 was efficiently knocked down in two MtPr10-1i lines. Moreover, five additional PR-10-type proteins annotated as abscisic acid responsive proteins (ABR17s) revealed also an almost complete silencing in these two lines. Inoculation of the root cultures with the oomycete root pathogen A. euteiches resulted in a clearly reduced colonization and thus in a suppressed infection development in MtPr10-1i roots as compared to that in roots of the transformation controls. In addition, MtPr10-1 silencing led to the induction of a new set of PR proteins after infection with A. euteiches including the de novo induction of two isoforms of thaumatin-like proteins (PR-5b), which were not detectable in A. euteiches-infected control roots. Thus, antagonistic induction of other PR proteins, which are normally repressed due to PR-10 expression, is supposed to cause an increased resistance of M. truncatula upon an A. euteiches in vitro infection. The results were also further confirmed by detecting increased PR-5b induction levels in 2-D gels of a previously analyzed M. truncatula line (F83.005-9) exhibiting increased A. euteiches tolerance associated with reduced PR-10 induction levels.
Stichworte
Medicago; proteomics; RNAi; Aphanomyces; Pathogenesis-related protein 10 (PR-10); Pathogenesis-related protein 5b (PR-5b)
Erscheinungsjahr
2007
Zeitschriftentitel
Planta
Band
226
Ausgabe
1
Seite(n)
57-71
ISSN
0032-0935
eISSN
1432-2048
Page URI
https://pub.uni-bielefeld.de/record/1593805

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Colditz F, Niehaus K, Krajinski F. Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta. 2007;226(1):57-71.
Colditz, F., Niehaus, K., & Krajinski, F. (2007). Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta, 226(1), 57-71. https://doi.org/10.1007/s00425-006-0466-y
Colditz, Frank, Niehaus, Karsten, and Krajinski, Franziska. 2007. “Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches”. Planta 226 (1): 57-71.
Colditz, F., Niehaus, K., and Krajinski, F. (2007). Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta 226, 57-71.
Colditz, F., Niehaus, K., & Krajinski, F., 2007. Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta, 226(1), p 57-71.
F. Colditz, K. Niehaus, and F. Krajinski, “Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches”, Planta, vol. 226, 2007, pp. 57-71.
Colditz, F., Niehaus, K., Krajinski, F.: Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta. 226, 57-71 (2007).
Colditz, Frank, Niehaus, Karsten, and Krajinski, Franziska. “Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches”. Planta 226.1 (2007): 57-71.

36 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions.
Zhang Q, Gao X, Ren Y, Ding X, Qiu J, Li N, Zeng F, Chu Z., Int J Mol Sci 19(1), 2018
PMID: 29342876
Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance.
Eissa HF, Hassanien SE, Ramadan AM, El-Shamy MM, Saleh OM, Shokry AM, Abdelsattar M, Morsy YB, El-Maghraby MA, Alameldin HF, Hassan SM, Osman GH, Mahfouz HT, Gad El-Karim GA, Madkour MA, Bahieldin A., Plant Methods 13(), 2017
PMID: 28539970
Improved Shoot Regeneration, Salinity Tolerance and Reduced Fungal Susceptibility in Transgenic Tobacco Constitutively Expressing PR-10a Gene.
Agarwal P, Dabi M, More P, Patel K, Jana K, Agarwal PK., Front Plant Sci 7(), 2016
PMID: 26973666
Cross-Regulation between N Metabolism and Nitric Oxide (NO) Signaling during Plant Immunity.
Thalineau E, Truong HN, Berger A, Fournier C, Boscari A, Wendehenne D, Jeandroz S., Front Plant Sci 7(), 2016
PMID: 27092169
A Novel Pathogenesis-Related Class 10 Protein Gly m 4l, Increases Resistance upon Phytophthora sojae Infection in Soybean (Glycine max [L.] Merr.).
Fan S, Jiang L, Wu J, Dong L, Cheng Q, Xu P, Zhang S., PLoS One 10(10), 2015
PMID: 26474489
Proteomic investigation of response to FORL infection in tomato roots.
Mazzeo MF, Cacace G, Ferriello F, Puopolo G, Zoina A, Ercolano MR, Siciliano RA., Plant Physiol Biochem 74(), 2014
PMID: 24262994
Pathogenesis related-10 proteins are small, structurally similar but with diverse role in stress signaling.
Agarwal P, Agarwal PK., Mol Biol Rep 41(2), 2014
PMID: 24343423
The alternative Medicago truncatula defense proteome of ROS-defective transgenic roots during early microbial infection.
Kiirika LM, Schmitz U, Colditz F., Front Plant Sci 5(), 2014
PMID: 25101099
Differential responses of three grapevine cultivars to Botryosphaeria dieback.
Spagnolo A, Magnin-Robert M, Alayi TD, Cilindre C, Schaeffer-Reiss C, Van Dorsselaer A, Clément C, Larignon P, Ramirez-Suero M, Chong J, Bertsch C, Abou-Mansour E, Fontaine F., Phytopathology 104(10), 2014
PMID: 24724741
Structural and functional aspects of PR-10 proteins.
Fernandes H, Michalska K, Sikorski M, Jaskolski M., FEBS J 280(5), 2013
PMID: 23289796
The Mitochondrial Complexome of Medicago truncatula.
Kiirika LM, Behrens C, Braun HP, Colditz F., Front Plant Sci 4(), 2013
PMID: 23596449
Impacts of pr-10a overexpression at the molecular and the phenotypic level.
Vaas LA, Marheine M, Sikorski J, Göker M, Schumacher HM., Int J Mol Sci 14(7), 2013
PMID: 23880863
The influence of the fungal pathogen Mycocentrospora acerina on the proteome and polyacetylenes and 6-methoxymellein in organic and conventionally cultivated carrots (Daucus carota) during post harvest storage.
Louarn S, Nawrocki A, Edelenbos M, Jensen DF, Jensen ON, Collinge DB, Jensen B., J Proteomics 75(3), 2012
PMID: 22075166
Phoma medicaginis stimulates the induction of the octadecanoid and phenylpropanoid pathways in Medicago truncatula.
Kamphuis LG, Williams AH, Küster H, Trengove RD, Singh KB, Oliver RP, Ellwood SR., Mol Plant Pathol 13(6), 2012
PMID: 22212347
Silencing of the Rac1 GTPase MtROP9 in Medicago truncatula stimulates early mycorrhizal and oomycete root colonizations but negatively affects rhizobial infection.
Kiirika LM, Bergmann HF, Schikowsky C, Wimmer D, Korte J, Schmitz U, Niehaus K, Colditz F., Plant Physiol 159(1), 2012
PMID: 22399646
Requirement of the cytosolic interaction between PATHOGENESIS-RELATED PROTEIN10 and LEUCINE-RICH REPEAT PROTEIN1 for cell death and defense signaling in pepper.
Choi DS, Hwang IS, Hwang BK., Plant Cell 24(4), 2012
PMID: 22492811
Dau c 1.01 and Dau c 1.02-silenced transgenic carrot plants show reduced allergenicity to patients with carrot allergy.
Peters S, Imani J, Mahler V, Foetisch K, Kaul S, Paulus KE, Scheurer S, Vieths S, Kogel KH., Transgenic Res 20(3), 2011
PMID: 20798987
Expression of coordinately regulated defence response genes and analysis of their role in disease resistance in Medicago truncatula.
Samac DA, Peñuela S, Schnurr JA, Hunt EN, Foster-Hartnett D, Vandenbosch KA, Gantt JS., Mol Plant Pathol 12(8), 2011
PMID: 21726379
Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae.
Wang FX, Ma YP, Yang CL, Zhao PM, Yao Y, Jian GL, Luo YM, Xia GX., Proteomics 11(22), 2011
PMID: 21928292
Differential gel electrophoresis (DIGE) to quantitatively monitor early symbiosis- and pathogenesis-induced changes of the Medicago truncatula root proteome.
Schenkluhn L, Hohnjec N, Niehaus K, Schmitz U, Colditz F., J Proteomics 73(4), 2010
PMID: 19895911
Expression profiling of a complex thaumatin-like protein family in western white pine.
Liu JJ, Zamani A, Ekramoddoullah AK., Planta 231(3), 2010
PMID: 19997927
A complex genetic network involving a broad-spectrum locus and strain-specific loci controls resistance to different pathotypes of Aphanomyces euteiches in Medicago truncatula.
Hamon C, Baranger A, Miteul H, Lecointe R, Le Goff I, Deniot G, Onfroy C, Moussart A, Prosperi JM, Tivoli B, Delourme R, Pilet-Nayel ML., Theor Appl Genet 120(5), 2010
PMID: 20012740
The MtSNF4b subunit of the sucrose non-fermenting-related kinase complex connects after-ripening and constitutive defense responses in seeds of Medicago truncatula.
Bolingue W, Rosnoblet C, Leprince O, Vu BL, Aubry C, Buitink J., Plant J 61(5), 2010
PMID: 20015062
A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis.
Laporte P, Satiat-Jeunemaître B, Velasco I, Csorba T, Van de Velde W, Campalans A, Burgyan J, Arevalo-Rodriguez M, Crespi M., Plant J 62(1), 2010
PMID: 20042020
Expression and functional characterization of two pathogenesis-related protein 10 genes from Zea mays.
Xie YR, Chen ZY, Brown RL, Bhatnagar D., J Plant Physiol 167(2), 2010
PMID: 19682768
PR10 expression in maize and its effect on host resistance against Aspergillus flavus infection and aflatoxin production.
Chen ZY, Brown RL, Damann KE, Cleveland TE., Mol Plant Pathol 11(1), 2010
PMID: 20078777
Model legumes contribute to faba bean breeding
Rispail Nicolas, Kaló Péter, Kiss GyörgyB, Ellis THNoel, Gallardo Karine, Thompson RichardD, Prats Elena, Larrainzar Estibaliz, Ladrera Ruben, González EstherM, Arrese-Igor Cesar, Ferguson BrettJ, Gresshoff PeterM, Rubiales Diego., Field Crops Res 115(3), 2010
PMID: IND44316555
A study on the susceptibility of the model legume plant Medicago truncatula to the soil-borne pathogen Fusarium oxysporum
Ramírez-Suero M, Khanshour A, Martinez Y, Rickauer M., Eur J Plant Pathol 126(4), 2010
PMID: IND44336452
Proteomics of plant pathogenic fungi.
González-Fernández R, Prats E, Jorrín-Novo JV., J Biomed Biotechnol 2010(), 2010
PMID: 20589070
The B-3 ethylene response factor MtERF1-1 mediates resistance to a subset of root pathogens in Medicago truncatula without adversely affecting symbiosis with rhizobia.
Anderson JP, Lichtenzveig J, Gleason C, Oliver RP, Singh KB., Plant Physiol 154(2), 2010
PMID: 20713618
Comparative proteomic studies of root-microbe interactions.
Mathesius U., J Proteomics 72(3), 2009
PMID: 19152841
High-affinity copper transport and Snq2 export permease of saccharomyces cerevisiae modulate cytotoxicity of PR-10 from Theobroma cacao.
Pungartnik C, da Silva AC, de Melo SA, Gramacho KP, de Mattos Cascardo JC, Brendel M, Micheli F, da Silva Gesteira A., Mol Plant Microbe Interact 22(1), 2009
PMID: 19061401
Expression of the SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1) gene is associated with developmental change in the life cycle of the model legume Medicago truncatula.
Nolan KE, Kurdyukov S, Rose RJ., J Exp Bot 60(6), 2009
PMID: 19305022
Lupinus luteus pathogenesis-related protein as a reservoir for cytokinin.
Fernandes H, Pasternak O, Bujacz G, Bujacz A, Sikorski MM, Jaskolski M., J Mol Biol 378(5), 2008
PMID: 18406424
ARCHIPELAGO: a dedicated resource for exploiting past, present, and future genomic data on disease resistance regulation in rice.
Vergne E, Ballini E, Droc G, Tharreau D, Nottéghem JL, Morel JB., Mol Plant Microbe Interact 21(7), 2008
PMID: 18533828
Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus).
Hukkanen A, Kokko H, Buchala A, Häyrinen J, Kärenlampi S., Mol Plant Pathol 9(6), 2008
PMID: 19019008

37 References

Daten bereitgestellt von Europe PubMed Central.


LR, Plant Sci 118(), 1996

PRJ, Trends Microbiol 14(), 2005
Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations.
Boisson-Dernier A, Chabaud M, Garcia F, Becard G, Rosenberg C, Barker DG., Mol. Plant Microbe Interact. 14(6), 2001
PMID: 11386364
Local expression of enzymatically active class I beta-1, 3-glucanase enhances symptoms of TMV infection in tobacco.
Bucher GL, Tarina C, Heinlein M, Di Serio F, Meins F Jr, Iglesias VA., Plant J. 28(3), 2001
PMID: 11722778

M-M, Am Potato J 70(), 1993
Proteomic approach: identification of Medicago truncatula proteins induced in roots after infection with the pathogenic oomycete Aphanomyces euteiches.
Colditz F, Nyamsuren O, Niehaus K, Eubel H, Braun HP, Krajinski F., Plant Mol. Biol. 55(1), 2004
PMID: 15604668
Proteomic profiling unravels insights into the molecular background underlying increased Aphanomyces euteiches-tolerance of Medicago truncatula.
Colditz F, Braun HP, Jacquet C, Niehaus K, Krajinski F., Plant Mol. Biol. 59(3), 2005
PMID: 16235107
Transgenic potato plants overexpressing the pathogenesis-related STH-2 gene show unaltered susceptibility to Phytophthora infestans and potato virus X.
Constabel CP, Bertrand C, Brisson N., Plant Mol. Biol. 22(5), 1993
PMID: 8358029

DW, HortScience 30(), 1985

SL, Plant Mol Biol Rep 1(), 1983
Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5.
Fagoaga C, Rodrigo I, Conejero V, Hinarejos C, Tuset JJ, Arnau J, Pina JA, Navarro L, Pena L., Mol. Breed. 7(2), 2001
PMID: IND23227844

DJ, 1989

T, Z Naturforsch 56(), 2001
Proteome reference maps of Medicago truncatula embryogenic cell cultures generated from single protoplasts.
Imin N, De Jong F, Mathesius U, van Noorden G, Saeed NA, Wang XD, Rose RJ, Rolfe BG., Proteomics 4(7), 2004
PMID: 15221745
RNA interference identifies a calcium-dependent protein kinase involved in Medicago truncatula root development.
Ivashuta S, Liu J, Liu J, Lohar DP, Haridas S, Bucciarelli B, VandenBosch KA, Vance CP, Harrison MJ, Gantt JS., Plant Cell 17(11), 2005
PMID: 16199614
Molecular genetics of pathogenic oomycetes.
Kamoun S., Eukaryotic Cell 2(2), 2003
PMID: 12684368

JP, Eur J Plant Path 109(), 2003
RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula.
Limpens E, Ramos J, Franken C, Raz V, Compaan B, Franssen H, Bisseling T, Geurts R., J. Exp. Bot. 55(399), 2004
PMID: 15073217
Crystal structure of a hypoallergenic isoform of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier.
Markovic-Housley Z, Degano M, Lamba D, von Roepenack-Lahaye E, Clemens S, Susani M, Ferreira F, Scheiner O, Breiteneder H., J. Mol. Biol. 325(1), 2003
PMID: 12473456
Antifungal Hydrolases in Pea Tissue : II. Inhibition of Fungal Growth by Combinations of Chitinase and beta-1,3-Glucanase.
Mauch F, Mauch-Mani B, Boller T., Plant Physiol. 88(3), 1988
PMID: 16666407
High sequence similarity between a ribonuclease from ginseng calluses and fungus-elicited proteins from parsley indicates that intracellular pathogenesis-related proteins are ribonucleases.
Moiseyev GP, Beintema JJ, Fedoreyeva LI, Yakovlev GI., Planta 193(3), 1994
PMID: 7764876
Osmotin is a homolog of mammalian adiponectin and controls apoptosis in yeast through a homolog of mammalian adiponectin receptor.
Narasimhan ML, Coca MA, Jin J, Yamauchi T, Ito Y, Kadowaki T, Kim KK, Pardo JM, Damsz B, Hasegawa PM, Yun DJ, Bressan RA., Mol. Cell 17(2), 2005
PMID: 15664187

O, Physiol Mol Plant Path 63(), 2003
Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway.
Park CJ, Kim KJ, Shin R, Park JM, Shin YC, Paek KH., Plant J. 37(2), 2004
PMID: 14690503

AUTHOR UNKNOWN, 0

J-M, Grain Legumes 28(), 2000

J-M, 2001

HJ, Mol Plant Microbe Interact 6(), 1993

H, Plant Sci 165(), 2003
Characteristics of RNA silencing in plants: similarities and differences across kingdoms.
Susi P, Hohkuri M, Wahlroos T, Kilby NJ., Plant Mol. Biol. 54(2), 2004
PMID: 15159620
Several thaumatin-like proteins bind to beta-1,3-glucans.
Trudel J, Grenier J, Potvin C, Asselin A., Plant Physiol. 118(4), 1998
PMID: 9847118
The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins.
Loon LCvan, Strien EAvan., Physiol. Mol. Plant Pathol. 55(2), 1999
PMID: IND22038708

LC, Plant Mol Biol Rep 12(), 1994
A retrospective of an unconventionally trained plant pathologist: plant diseases to molecular plant pathology.
Ouchi S., Annu Rev Phytopathol 44(), 2006
PMID: 16602947

X, Proc Natl Acad Sci USA 93(), 1996

Y, Mol Plant Microbe Interact 12(), 1999
The potential of Betv1 homologues, a nuclear multigene family, as phylogenetic markers in flowering plants.
Wen J, Vanek-Krebitz M, Hoffmann-Sommergruber K, Scheiner O, Breiteneder H., Mol. Phylogenet. Evol. 8(3), 1997
PMID: 9417891
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