Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches

Colditz F, Niehaus K, Krajinski F (2007)
Planta 226(1): 57-71.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Colditz, Frank; Niehaus, KarstenUniBi; Krajinski, Franziska
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe K. Niehaus
Fakultät für Biologie > Proteom- und Metabolomforschung
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Abstract / Bemerkung
Recent studies on the root proteome of Medicago truncatula (Gaertn.) showed an induction of pathogenesis-related (PR) proteins of the class 10 after infection with the oomycete pathogen Aphanomyces euteiches (Drechs.). To get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using RNAi was carried out. Agrobacterium rhizogenes-mediated transformation of M. truncatula roots led to a knockdown of the Medicago PR10-1 gene in transgenic in vitro root cultures. Proteomic analyses of the MtPr10-1i root cultures showed that MtPr10-1 was efficiently knocked down in two MtPr10-1i lines. Moreover, five additional PR-10-type proteins annotated as abscisic acid responsive proteins (ABR17s) revealed also an almost complete silencing in these two lines. Inoculation of the root cultures with the oomycete root pathogen A. euteiches resulted in a clearly reduced colonization and thus in a suppressed infection development in MtPr10-1i roots as compared to that in roots of the transformation controls. In addition, MtPr10-1 silencing led to the induction of a new set of PR proteins after infection with A. euteiches including the de novo induction of two isoforms of thaumatin-like proteins (PR-5b), which were not detectable in A. euteiches-infected control roots. Thus, antagonistic induction of other PR proteins, which are normally repressed due to PR-10 expression, is supposed to cause an increased resistance of M. truncatula upon an A. euteiches in vitro infection. The results were also further confirmed by detecting increased PR-5b induction levels in 2-D gels of a previously analyzed M. truncatula line (F83.005-9) exhibiting increased A. euteiches tolerance associated with reduced PR-10 induction levels.
Stichworte
Medicago; proteomics; RNAi; Aphanomyces; Pathogenesis-related protein 10 (PR-10); Pathogenesis-related protein 5b (PR-5b)
Erscheinungsjahr
2007
Zeitschriftentitel
Planta
Band
226
Ausgabe
1
Seite(n)
57-71
ISSN
0032-0935
eISSN
1432-2048
Page URI
https://pub.uni-bielefeld.de/record/1593805

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Colditz F, Niehaus K, Krajinski F. Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta. 2007;226(1):57-71.
Colditz, F., Niehaus, K., & Krajinski, F. (2007). Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta, 226(1), 57-71. https://doi.org/10.1007/s00425-006-0466-y
Colditz, Frank, Niehaus, Karsten, and Krajinski, Franziska. 2007. “Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches”. Planta 226 (1): 57-71.
Colditz, F., Niehaus, K., and Krajinski, F. (2007). Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta 226, 57-71.
Colditz, F., Niehaus, K., & Krajinski, F., 2007. Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta, 226(1), p 57-71.
F. Colditz, K. Niehaus, and F. Krajinski, “Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches”, Planta, vol. 226, 2007, pp. 57-71.
Colditz, F., Niehaus, K., Krajinski, F.: Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches. Planta. 226, 57-71 (2007).
Colditz, Frank, Niehaus, Karsten, and Krajinski, Franziska. “Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches”. Planta 226.1 (2007): 57-71.

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