Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti

Bartels FW, McIntosh M, Fuhrmann A, Metzendorf C, Plattner P, Sewald N, Anselmetti D, Ros R, Becker A (2007)
Biophysical Journal 92(12): 4391-4400.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Bartels, Frank Wilco; McIntosh, Matthew; Fuhrmann, Alexander; Metzendorf, Christoph; Plattner, Patrik; Sewald, NorbertUniBi ; Anselmetti, DarioUniBi ; Ros, Robert; Becker, Anke
Abstract / Bemerkung
Intercellular communication by means of small signal molecules coordinates gene expression among bacteria. This population density-dependent regulation is known as quorum sensing. The symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti Rm1021 possesses the Sin quorum sensing system based on N-acyl homoserine lactones (AHL) as signal molecules. Here, we demonstrate that the LuxR-type regulator ExpR binds specifically to a target sequence in the sinRI locus in the presence of different AHLs with acyl side chains from 8 to 20 carbons. Dynamic force spectroscopy based on the atomic force microscope provided detailed information about the molecular mechanism of binding upon activation by six different AHLs. These single molecule experiments revealed that the mean lifetime of the bound protein-DNA complex varies depending on the specific effector molecule. The small differences between individual AHLs also had a pronounced influence on the structure of protein-DNA interaction: The reaction length of dissociation varied from 2.6 to 5.8 angstrom. In addition, dynamic force spectroscopy experiments indicate that N-heptanoyl-DL-homoserine lactone binds to ExpR but is not able to stimulate protein-DNA interaction.
Biophysical Journal
Page URI


Bartels FW, McIntosh M, Fuhrmann A, et al. Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti. Biophysical Journal. 2007;92(12):4391-4400.
Bartels, F. W., McIntosh, M., Fuhrmann, A., Metzendorf, C., Plattner, P., Sewald, N., Anselmetti, D., et al. (2007). Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti. Biophysical Journal, 92(12), 4391-4400. https://doi.org/10.1529/biophysj.106.082016
Bartels, F. W., McIntosh, M., Fuhrmann, A., Metzendorf, C., Plattner, P., Sewald, N., Anselmetti, D., Ros, R., and Becker, A. (2007). Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti. Biophysical Journal 92, 4391-4400.
Bartels, F.W., et al., 2007. Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti. Biophysical Journal, 92(12), p 4391-4400.
F.W. Bartels, et al., “Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti”, Biophysical Journal, vol. 92, 2007, pp. 4391-4400.
Bartels, F.W., McIntosh, M., Fuhrmann, A., Metzendorf, C., Plattner, P., Sewald, N., Anselmetti, D., Ros, R., Becker, A.: Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti. Biophysical Journal. 92, 4391-4400 (2007).
Bartels, Frank Wilco, McIntosh, Matthew, Fuhrmann, Alexander, Metzendorf, Christoph, Plattner, Patrik, Sewald, Norbert, Anselmetti, Dario, Ros, Robert, and Becker, Anke. “Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system in Sinorhizobium meliloti”. Biophysical Journal 92.12 (2007): 4391-4400.

24 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Regulation Mediated by N-Acyl Homoserine Lactone Quorum Sensing Signals in the Rhizobium-Legume Symbiosis.
Calatrava-Morales N, McIntosh M, Soto MJ., Genes (Basel) 9(5), 2018
PMID: 29783703
Single-Molecule Interactions of a Monoclonal Anti-DNA Antibody with DNA.
Nevzorova TA, Zhao Q, Lomakin YA, Ponomareva AA, Mukhitov AR, Purohit PK, Weisel JW, Litvinov RI., Bionanoscience 7(1), 2017
PMID: 29104846
Arrhythmogenic cardiomyopathy related DSG2 mutations affect desmosomal cadherin binding kinetics.
Dieding M, Debus JD, Kerkhoff R, Gaertner-Rommel A, Walhorn V, Milting H, Anselmetti D., Sci Rep 7(1), 2017
PMID: 29062102
Modulation of Sinorhizobium meliloti quorum sensing by Hfq-mediated post-transcriptional regulation of ExpR.
Gao M, Tang M, Guerich L, Salas-Gonzalez I, Teplitski M., Environ Microbiol Rep 7(1), 2015
PMID: 25382642
Catch bond interaction between cell-surface sulfatase Sulf1 and glycosaminoglycans.
Harder A, Möller AK, Milz F, Neuhaus P, Walhorn V, Dierks T, Anselmetti D., Biophys J 108(7), 2015
PMID: 25863062
Generic metric to quantify quorum sensing activation dynamics.
Pai A, Srimani JK, Tanouchi Y, You L., ACS Synth Biol 3(4), 2014
PMID: 24011134
Analysis of DNA interactions using single-molecule force spectroscopy.
Ritzefeld M, Walhorn V, Anselmetti D, Sewald N., Amino Acids 44(6), 2013
PMID: 23468137
Temporal expression program of quorum sensing-based transcription regulation in Sinorhizobium meliloti.
Charoenpanich P, Meyer S, Becker A, McIntosh M., J Bacteriol 195(14), 2013
PMID: 23687265
Direct force measurement of single DNA-peptide interactions using atomic force microscopy.
Chung JW, Shin D, Kwak JM, Seog J., J Mol Recognit 26(6), 2013
PMID: 23595808
Long lifetime of hydrogen-bonded DNA basepairs by force spectroscopy.
Fuhrmann A, Getfert S, Fu Q, Reimann P, Lindsay S, Ros R., Biophys J 102(10), 2012
PMID: 22677392
Application of atomic force microscopy for characteristics of single intermolecular interactions.
Safenkova IV, Zherdev AV, Dzantievf BB., Biochemistry (Mosc) 77(13), 2012
PMID: 23379527
Single-molecule force spectroscopy of cartilage aggrecan self-adhesion.
Harder A, Walhorn V, Dierks T, Fernàndez-Busquets X, Anselmetti D., Biophys J 99(10), 2010
PMID: 21081100
LuxR-family 'solos': bachelor sensors/regulators of signalling molecules.
Subramoni S, Venturi V., Microbiology 155(pt 5), 2009
PMID: 19383698
Quantitative analysis of single-molecule RNA-protein interaction.
Fuhrmann A, Schoening JC, Anselmetti D, Staiger D, Ros R., Biophys J 96(12), 2009
PMID: 19527663
Refined procedure of evaluating experimental single-molecule force spectroscopy data.
Fuhrmann A, Anselmetti D, Ros R, Getfert S, Reimann P., Phys Rev E Stat Nonlin Soft Matter Phys 77(3 pt 1), 2008
PMID: 18517427
Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility.
Bahlawane C, McIntosh M, Krol E, Becker A., Mol Plant Microbe Interact 21(11), 2008
PMID: 18842098

45 References

Daten bereitgestellt von Europe PubMed Central.

Quorum sensing in nitrogen-fixing rhizobia.
Gonzalez JE, Marketon MM., Microbiol. Mol. Biol. Rev. 67(4), 2003
PMID: 14665677
Quorum sensing as a population-density-dependent determinant of bacterial physiology.
Swift S, Downie JA, Whitehead NA, Barnard AM, Salmond GP, Williams P., Adv. Microb. Physiol. 45(), 2001
PMID: 11450110
Regulation of gene expression by cell-to-cell communication: acyl-homoserine lactone quorum sensing.
Fuqua C, Parsek MR, Greenberg EP., Annu. Rev. Genet. 35(), 2001
PMID: 11700290
Active efflux and diffusion are involved in transport of Pseudomonas aeruginosa cell-to-cell signals.
Pearson JP, Van Delden C, Iglewski BH., J. Bacteriol. 181(4), 1999
PMID: 9973347
Identification of two quorum-sensing systems in Sinorhizobium meliloti.
Marketon MM, Gonzalez JE., J. Bacteriol. 184(13), 2002
PMID: 12057940
Characterization of the Sinorhizobium meliloti sinR/sinI locus and the production of novel N-acyl homoserine lactones.
Marketon MM, Gronquist MR, Eberhard A, Gonzalez JE., J. Bacteriol. 184(20), 2002
PMID: 12270827
A LuxR homolog controls production of symbiotically active extracellular polysaccharide II by Sinorhizobium meliloti.
Pellock BJ, Teplitski M, Boinay RP, Bauer WD, Walker GC., J. Bacteriol. 184(18), 2002
PMID: 12193623
The crystal structure of the quorum sensing protein TraR bound to its autoinducer and target DNA.
Vannini A, Volpari C, Gargioli C, Muraglia E, Cortese R, De Francesco R, Neddermann P, Marco SD., EMBO J. 21(17), 2002
PMID: 12198141
Quorum sensing controls exopolysaccharide production in Sinorhizobium meliloti.
Marketon MM, Glenn SA, Eberhard A, Gonzalez JE., J. Bacteriol. 185(1), 2003
PMID: 12486070
sinI- and expR-dependent quorum sensing in Sinorhizobium meliloti.
Gao M, Chen H, Eberhard A, Gronquist MR, Robinson JB, Rolfe BG, Bauer WD., J. Bacteriol. 187(23), 2005
PMID: 16291666
Grabbing the cat by the tail: manipulating molecules one by one.
Bustamante C, Macosko JC, Wuite GJ., Nat. Rev. Mol. Cell Biol. 1(2), 2000
PMID: 11253365
Direct measurement of the forces between complementary strands of DNA.
Lee GU, Chrisey LA, Colton RJ., Science 266(5186), 1994
PMID: 7973628
Adhesion forces between individual ligand-receptor pairs.
Florin EL, Moy VT, Gaub HE., Science 264(5157), 1994
PMID: 8153628
Detection and localization of individual antibody-antigen recognition events by atomic force microscopy.
Hinterdorfer P, Baumgartner W, Gruber HJ, Schilcher K, Schindler H., Proc. Natl. Acad. Sci. U.S.A. 93(8), 1996
PMID: 8622961
Specific antigen/antibody interactions measured by force microscopy.
Dammer U, Hegner M, Anselmetti D, Wagner P, Dreier M, Huber W, Guntherodt HJ., Biophys. J. 70(5), 1996
PMID: 9172770
Antigen binding forces of individually addressed single-chain Fv antibody molecules.
Ros R, Schwesinger F, Anselmetti D, Kubon M, Schafer R, Pluckthun A, Tiefenauer L., Proc. Natl. Acad. Sci. U.S.A. 95(13), 1998
PMID: 9636161
Mechanical switching and coupling between two dissociation pathways in a P-selectin adhesion bond.
Evans E, Leung A, Heinrich V, Zhu C., Proc. Natl. Acad. Sci. U.S.A. 101(31), 2004
PMID: 15277675
Binding strength between cell adhesion proteoglycans measured by atomic force microscopy.
Dammer U, Popescu O, Wagner P, Anselmetti D, Guntherodt HJ, Misevic GN., Science 267(5201), 1995
PMID: 7855599
Stochastic resonance driven by time-modulated neurotransmitter random point trains.
Zhangcai L, Youguo Q., Phys. Rev. Lett. 91(20), 2003
PMID: 14683401
LexA-DNA bond strength by single molecule force spectroscopy.
Kuhner F, Costa LT, Bisch PM, Thalhammer S, Heckl WM, Gaub HE., Biophys. J. 87(4), 2004
PMID: 15454462
Detailed studies of the binding mechanism of the Sinorhizobium meliloti transcriptional activator ExpG to DNA.
Baumgarth B, Bartels FW, Anselmetti D, Becker A, Ros R., Microbiology (Reading, Engl.) 151(Pt 1), 2005
PMID: 15632443
Single-molecule experiments in synthetic biology: an approach to the affinity ranking of DNA-binding peptides.
Eckel R, Wilking SD, Becker A, Sewald N, Ros R, Anselmetti D., Angew. Chem. Int. Ed. Engl. 44(25), 2005
PMID: 15906400
Dynamic strength of molecular adhesion bonds.
Evans E, Ritchie K., Biophys. J. 72(4), 1997
PMID: 9083660
Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy.
Merkel R, Nassoy P, Leung A, Ritchie K, Evans E., Nature 397(6714), 1999
PMID: 9892352
R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098


Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions.
Becker A, Berges H, Krol E, Bruand C, Ruberg S, Capela D, Lauber E, Meilhoc E, Ampe F, de Bruijn FJ, Fourment J, Francez-Charlot A, Kahn D, Kuster H, Liebe C, Puhler A, Weidner S, Batut J., Mol. Plant Microbe Interact. 17(3), 2004
PMID: 15000396

Critical regions of the Vibrio fischeri luxR protein defined by mutational analysis.
Slock J, VanRiet D, Kolibachuk D, Greenberg EP., J. Bacteriol. 172(7), 1990
PMID: 2361947
The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products.
Becker A, Ruberg S, Kuster H, Roxlau AA, Keller M, Ivashina T, Cheng HP, Walker GC, Puhler A., J. Bacteriol. 179(4), 1997
PMID: 9023225
Reversible acyl-homoserine lactone binding to purified Vibrio fischeri LuxR protein.
Urbanowski ML, Lostroh CP, Greenberg EP., J. Bacteriol. 186(3), 2004
PMID: 14729687
Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein.
Manefield M, de Nys R, Kumar N, Read R, Givskov M, Steinberg P, Kjelleberg S., Microbiology (Reading, Engl.) 145 ( Pt 2)(), 1999
PMID: 10075410
The regulatory locus cinRI in Rhizobium leguminosarum controls a network of quorum-sensing loci.
Lithgow JK, Wilkinson A, Hardman A, Rodelas B, Wisniewski-Dye F, Williams P, Downie JA., Mol. Microbiol. 37(1), 2000
PMID: 10931307
Chemical identification of N-acyl homoserine lactone quorum-sensing signals produced by Sinorhizobium meliloti strains in defined medium.
Teplitski M, Eberhard A, Gronquist MR, Gao M, Robinson JB, Bauer WD., Arch. Microbiol. 180(6), 2003
PMID: 14593447
Use of Sinorhizobium meliloti as an indicator for specific detection of long-chain N-acyl homoserine lactones.
Llamas I, Keshavan N, Gonzalez JE., Appl. Environ. Microbiol. 70(6), 2004
PMID: 15184178


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 17384071
PubMed | Europe PMC

Suchen in

Google Scholar