Fluorescence of single molecules in polymer films: Sensitivity of blinking to local environment

Clifford JN, Bell TDM, Tinnefeld P, Heilemann M, Melnikov SM, Hotta J-ichi, Sliwa M, Dedecker P, Sauer M, Hofkens J, Yeow EKL (2007)
JOURNAL OF PHYSICAL CHEMISTRY B 111(25): 6987-6991.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ; ; ; ; ; ;
Abstract / Bemerkung
The single-molecule fluorescence blinking behavior of the organic dye Atto647N in various polymer matrixes such as Zeonex, PVK, and PVA as well as aqueous media was investigated. Fluorescence blinking with off-times in the millisecond to second time range is assigned to dye radical ions formed by photoinduced electron transfer reactions from or to the environment. In Zeonex and PVK, the measured off-time distributions show power law dependence, whereas, in PVA, no such dependence is observed. Rather, in this polymer, off-time distributions can be best fitted to monoexponential or stretched exponential functions. Furthermore, treatment of PVA samples to mild heating and low pressure greatly reduces the frequency of blinking events. We tentatively ascribe this to the removal of water pockets within the polymer film itself. Measurements of the dye immobilized in water in the presence of methylviologen, a strongly oxidizing agent, reveal simple exponential on- and off-time distributions. Thus, our data suggest that the blinking behavior of single organic molecules is sensitive to their immediate environment and, moreover, that fluorescence blinking on- and off-time distributions do not inherently and uniquely obey a power law.
Erscheinungsjahr
2007
Zeitschriftentitel
JOURNAL OF PHYSICAL CHEMISTRY B
Band
111
Ausgabe
25
Seite(n)
6987-6991
ISSN
1520-6106
eISSN
1520-5207
Page URI
https://pub.uni-bielefeld.de/record/1593685

Zitieren

Clifford JN, Bell TDM, Tinnefeld P, et al. Fluorescence of single molecules in polymer films: Sensitivity of blinking to local environment. JOURNAL OF PHYSICAL CHEMISTRY B. 2007;111(25):6987-6991.
Clifford, J. N., Bell, T. D. M., Tinnefeld, P., Heilemann, M., Melnikov, S. M., Hotta, J. -ichi, Sliwa, M., et al. (2007). Fluorescence of single molecules in polymer films: Sensitivity of blinking to local environment. JOURNAL OF PHYSICAL CHEMISTRY B, 111(25), 6987-6991. doi:10.1021/jp072864d
Clifford, J. N., Bell, T. D. M., Tinnefeld, P., Heilemann, M., Melnikov, S. M., Hotta, J. -ichi, Sliwa, M., Dedecker, P., Sauer, M., Hofkens, J., et al. (2007). Fluorescence of single molecules in polymer films: Sensitivity of blinking to local environment. JOURNAL OF PHYSICAL CHEMISTRY B 111, 6987-6991.
Clifford, J.N., et al., 2007. Fluorescence of single molecules in polymer films: Sensitivity of blinking to local environment. JOURNAL OF PHYSICAL CHEMISTRY B, 111(25), p 6987-6991.
J.N. Clifford, et al., “Fluorescence of single molecules in polymer films: Sensitivity of blinking to local environment”, JOURNAL OF PHYSICAL CHEMISTRY B, vol. 111, 2007, pp. 6987-6991.
Clifford, J.N., Bell, T.D.M., Tinnefeld, P., Heilemann, M., Melnikov, S.M., Hotta, J.-ichi, Sliwa, M., Dedecker, P., Sauer, M., Hofkens, J., Yeow, E.K.L.: Fluorescence of single molecules in polymer films: Sensitivity of blinking to local environment. JOURNAL OF PHYSICAL CHEMISTRY B. 111, 6987-6991 (2007).
Clifford, John N., Bell, Toby D. M., Tinnefeld, Philip, Heilemann, Mike, Melnikov, Sergey M., Hotta, Jun-ichi, Sliwa, Michel, Dedecker, Peter, Sauer, Markus, Hofkens, Johan, and Yeow, Edwin K. L. “Fluorescence of single molecules in polymer films: Sensitivity of blinking to local environment”. JOURNAL OF PHYSICAL CHEMISTRY B 111.25 (2007): 6987-6991.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17530801
PubMed | Europe PMC

Suchen in

Google Scholar