Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system

Beshay U, Miksch G, Flaschel E (2007)
BIOPROCESS AND BIOSYSTEMS ENGINEERING 30(4): 251-259.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Activity tests of enzymes are often applied for determining their concentration. In the easiest case, just one product concentration is measured after a given time. This often leads to nonlinear dependences of the apparent activity with enzyme protein concentration. A general solution of this problem consists in using the balance equation of the assay system, which commonly represents a batch reactor. Here, the balance equation of the batch for a general Michaelis Menten-type reaction kinetics is used as the calibration function. The correlation of the apparent activity and enzyme concentration was established by capturing the enzyme by means of metal chelate interaction owing to a hexahistidine tag attached to the beta-glucanase.
Stichworte
batch reactor balance; reaction; kinetics; IMAC; beta-glucanase; enzyme activity
Erscheinungsjahr
2007
Zeitschriftentitel
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Band
30
Ausgabe
4
Seite(n)
251-259
ISSN
1615-7591
eISSN
1615-7605
Page URI
https://pub.uni-bielefeld.de/record/1593326

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Beshay U, Miksch G, Flaschel E. Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2007;30(4):251-259.
Beshay, U., Miksch, G., & Flaschel, E. (2007). Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 30(4), 251-259. doi:10.1007/s00449-007-0121-4
Beshay, U., Miksch, G., and Flaschel, E. (2007). Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING 30, 251-259.
Beshay, U., Miksch, G., & Flaschel, E., 2007. Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 30(4), p 251-259.
U. Beshay, G. Miksch, and E. Flaschel, “Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system”, BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 30, 2007, pp. 251-259.
Beshay, U., Miksch, G., Flaschel, E.: Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 30, 251-259 (2007).
Beshay, Usama, Miksch, Gerd, and Flaschel, Erwin. “Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system”. BIOPROCESS AND BIOSYSTEMS ENGINEERING 30.4 (2007): 251-259.

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PMID: 17351833
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