Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system
Beshay U, Miksch G, Flaschel E (2007)
BIOPROCESS AND BIOSYSTEMS ENGINEERING 30(4): 251-259.
Zeitschriftenaufsatz
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Autor*in
Beshay, Usama;
Miksch, GerdUniBi;
Flaschel, ErwinUniBi
Einrichtung
Abstract / Bemerkung
Activity tests of enzymes are often applied for determining their concentration. In the easiest case, just one product concentration is measured after a given time. This often leads to nonlinear dependences of the apparent activity with enzyme protein concentration. A general solution of this problem consists in using the balance equation of the assay system, which commonly represents a batch reactor. Here, the balance equation of the batch for a general Michaelis Menten-type reaction kinetics is used as the calibration function. The correlation of the apparent activity and enzyme concentration was established by capturing the enzyme by means of metal chelate interaction owing to a hexahistidine tag attached to the beta-glucanase.
Stichworte
batch reactor balance;
reaction;
kinetics;
IMAC;
beta-glucanase;
enzyme activity
Erscheinungsjahr
2007
Zeitschriftentitel
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Band
30
Ausgabe
4
Seite(n)
251-259
ISSN
1615-7591
eISSN
1615-7605
Page URI
https://pub.uni-bielefeld.de/record/1593326
Zitieren
Beshay U, Miksch G, Flaschel E. Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2007;30(4):251-259.
Beshay, U., Miksch, G., & Flaschel, E. (2007). Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 30(4), 251-259. https://doi.org/10.1007/s00449-007-0121-4
Beshay, Usama, Miksch, Gerd, and Flaschel, Erwin. 2007. “Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system”. BIOPROCESS AND BIOSYSTEMS ENGINEERING 30 (4): 251-259.
Beshay, U., Miksch, G., and Flaschel, E. (2007). Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING 30, 251-259.
Beshay, U., Miksch, G., & Flaschel, E., 2007. Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 30(4), p 251-259.
U. Beshay, G. Miksch, and E. Flaschel, “Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system”, BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 30, 2007, pp. 251-259.
Beshay, U., Miksch, G., Flaschel, E.: Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 30, 251-259 (2007).
Beshay, Usama, Miksch, Gerd, and Flaschel, Erwin. “Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system”. BIOPROCESS AND BIOSYSTEMS ENGINEERING 30.4 (2007): 251-259.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
4 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism.
Velur Selvamani RS, Friehs K, Flaschel E., Bioprocess Biosyst Eng 37(3), 2014
PMID: 23820825
Velur Selvamani RS, Friehs K, Flaschel E., Bioprocess Biosyst Eng 37(3), 2014
PMID: 23820825
Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA).
Velur Selvamani RS, Telaar M, Friehs K, Flaschel E., Microb Cell Fact 13(), 2014
PMID: 24745552
Velur Selvamani RS, Telaar M, Friehs K, Flaschel E., Microb Cell Fact 13(), 2014
PMID: 24745552
Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli.
Spexard M, Beshay U, Risse JM, Miksch G, Flaschel E., Biotechnol Lett 32(2), 2010
PMID: 19816658
Spexard M, Beshay U, Risse JM, Miksch G, Flaschel E., Biotechnol Lett 32(2), 2010
PMID: 19816658
Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli.
Beshay U, Miksch G, Friehs K, Flaschel E., Bioprocess Biosyst Eng 32(2), 2009
PMID: 18481103
Beshay U, Miksch G, Friehs K, Flaschel E., Bioprocess Biosyst Eng 32(2), 2009
PMID: 18481103
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