Periodic acceptor excitation spectroscopy of single molecules
Doose S, Heilemann M, Michalet X, Weiss S, Kapanidis AN (2007)
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 36(6): 669-674.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Doose, Soren;
Heilemann, MikeUniBi;
Michalet, Xavier;
Weiss, Shimon;
Kapanidis, Achillefs N.
Einrichtung
Abstract / Bemerkung
Alternating-laser excitation (ALEX) spectroscopy has recently been added to the single-molecule spectroscopy toolkit. ALEX monitors interaction and stoichiometry of biomolecules, reports on biomolecular structure by measuring accurate Forster resonance energy transfer (FRET) efficiencies, and allows sorting of subpopulations on the basis of stoichiometry and FRET. Here, we demonstrate that a simple combination of one continuous-wave donor-excitation laser and one directly modulated acceptor-excitation laser (Periodic Acceptor eXcitation) is sufficient to recapitulate the capabilities of ALEX while minimizing the cost and complexity associated with use of modulation techniques.
Stichworte
alternating-laser excitation;
single-molecule fluorescence spectroscopy;
(ALEX);
Forster resonance energy transfer (FRET);
biomolecular;
interactions
Erscheinungsjahr
2007
Zeitschriftentitel
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Band
36
Ausgabe
6
Seite(n)
669-674
ISSN
0175-7571
eISSN
1432-1017
Page URI
https://pub.uni-bielefeld.de/record/1593226
Zitieren
Doose S, Heilemann M, Michalet X, Weiss S, Kapanidis AN. Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 2007;36(6):669-674.
Doose, S., Heilemann, M., Michalet, X., Weiss, S., & Kapanidis, A. N. (2007). Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 36(6), 669-674. https://doi.org/10.1007/s00249-007-0133-7
Doose, Soren, Heilemann, Mike, Michalet, Xavier, Weiss, Shimon, and Kapanidis, Achillefs N. 2007. “Periodic acceptor excitation spectroscopy of single molecules”. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 36 (6): 669-674.
Doose, S., Heilemann, M., Michalet, X., Weiss, S., and Kapanidis, A. N. (2007). Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 36, 669-674.
Doose, S., et al., 2007. Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 36(6), p 669-674.
S. Doose, et al., “Periodic acceptor excitation spectroscopy of single molecules”, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, vol. 36, 2007, pp. 669-674.
Doose, S., Heilemann, M., Michalet, X., Weiss, S., Kapanidis, A.N.: Periodic acceptor excitation spectroscopy of single molecules. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 36, 669-674 (2007).
Doose, Soren, Heilemann, Mike, Michalet, Xavier, Weiss, Shimon, and Kapanidis, Achillefs N. “Periodic acceptor excitation spectroscopy of single molecules”. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 36.6 (2007): 669-674.
6 Zitationen in Europe PMC
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48-spot single-molecule FRET setup with periodic acceptor excitation.
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