FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI

Mironova OS, Budyak IL, Bueldt G, Schlesinger R, Heberle J (2007)
BIOCHEMISTRY 46(33): 9399-9405.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ;
Abstract / Bemerkung
The phototaxis receptor sensory rhodopsin I (SRI) from Halobacterium salinarum interacts with its cognate transducer (HtrI) forming a transmembrane complex. After light excitation of the chromophore all-trans retinal, SRI undergoes structural changes that are ultimately transmitted to HtrI. The interaction of SRI with HtrI results in the closure of the receptor's proton pathway, which renders the photocycle recovery kinetics of SRI pH-independent. We demonstrate on heterologously expressed and reconstituted SRI-HtrI fusion proteins that the transmembrane part of HtrI (residues 1-52) as well as the downstream cytoplasmic part (residues 53-147) exhibit conformational changes after light excitation. The sum of these conformational changes is similar to those observed in the fusion constructs SRI-HtrI 1-71 and SRI-HtrI 1-147, which display pH-independent receptor kinetics. These results indicate the occurrence of spatially distinct conformational changes that are required for functional signal transmission. Kinetic and spectroscopic analysis of HtrI point mutants of Asn53 provides evidence that this residue is involved in the receptor-transducer interaction. We suggest that Asn53 plays a role similar to that of Asn74 of the HtrII from Natronobacterium pharaonis, the latter forming a hydrogen bond to the receptor within the membrane.
Erscheinungsjahr
2007
Zeitschriftentitel
BIOCHEMISTRY
Band
46
Ausgabe
33
Seite(n)
9399-9405
ISSN
0006-2960
eISSN
1520-4995
Page URI
https://pub.uni-bielefeld.de/record/1593137

Zitieren

Mironova OS, Budyak IL, Bueldt G, Schlesinger R, Heberle J. FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI. BIOCHEMISTRY. 2007;46(33):9399-9405.
Mironova, O. S., Budyak, I. L., Bueldt, G., Schlesinger, R., & Heberle, J. (2007). FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI. BIOCHEMISTRY, 46(33), 9399-9405. doi:10.1021/bi700563f
Mironova, O. S., Budyak, I. L., Bueldt, G., Schlesinger, R., and Heberle, J. (2007). FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI. BIOCHEMISTRY 46, 9399-9405.
Mironova, O.S., et al., 2007. FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI. BIOCHEMISTRY, 46(33), p 9399-9405.
O.S. Mironova, et al., “FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI”, BIOCHEMISTRY, vol. 46, 2007, pp. 9399-9405.
Mironova, O.S., Budyak, I.L., Bueldt, G., Schlesinger, R., Heberle, J.: FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI. BIOCHEMISTRY. 46, 9399-9405 (2007).
Mironova, Olga S., Budyak, Ivan L., Bueldt, Georg, Schlesinger, Ramona, and Heberle, Joachim. “FT-IR difference spectroscopy elucidates crucial interactions of sensory rhodopsin I with the cognate transducer HtrI”. BIOCHEMISTRY 46.33 (2007): 9399-9405.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17655327
PubMed | Europe PMC

Suchen in

Google Scholar