Refined procedure of evaluating experimental single-molecule force spectroscopy data
Fuhrmann A, Anselmetti D, Ros R, Getfert S, Reimann P (2008)
PHYSICAL REVIEW E 77(3): 31912.
Zeitschriftenaufsatz
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Autor*in
Fuhrmann, Alexander;
Anselmetti, DarioUniBi ;
Ros, Robert;
Getfert, SebastianUniBi;
Reimann, PeterUniBi
Einrichtung
Fakultät für Physik > AG Biophysik und angewandte Nanowissenschaften
Centrum für Biotechnologie > Arbeitsgruppe D. Anselmetti
SFB 613 Physik von Einzelmolekülprozessen/mol.Erkennung in organ.Sys.
Centrum für Biotechnologie > Institut für Biophysik und Nanowissenschaften
Fakultät für Physik > AG Theorie der Kondensierten Materie
Centrum für Biotechnologie > Arbeitsgruppe D. Anselmetti
SFB 613 Physik von Einzelmolekülprozessen/mol.Erkennung in organ.Sys.
Centrum für Biotechnologie > Institut für Biophysik und Nanowissenschaften
Fakultät für Physik > AG Theorie der Kondensierten Materie
Abstract / Bemerkung
Dynamic force spectroscopy is a well-established tool to study molecular recognition in a wide range of binding affinities on the single-molecule level. The theoretical interpretation of these data is still very challenging and the models describe the experimental data only partly. In this paper we reconsider the basic assumptions of the models on the basis of an experimental data set and propose an approach of analyzing and quantitatively evaluating dynamic force spectroscopy data on single ligand-receptor complexes. We present our procedure to process and analyze the force-distance curves, to detect the rupture events in an automated manner, and to calculate quantitative parameters for a biophysical characterization of the investigated interaction.
Erscheinungsjahr
2008
Zeitschriftentitel
PHYSICAL REVIEW E
Band
77
Ausgabe
3
Seite(n)
31912
ISSN
1539-3755
eISSN
1550-2376
Page URI
https://pub.uni-bielefeld.de/record/1592084
Zitieren
Fuhrmann A, Anselmetti D, Ros R, Getfert S, Reimann P. Refined procedure of evaluating experimental single-molecule force spectroscopy data. PHYSICAL REVIEW E. 2008;77(3):31912.
Fuhrmann, A., Anselmetti, D., Ros, R., Getfert, S., & Reimann, P. (2008). Refined procedure of evaluating experimental single-molecule force spectroscopy data. PHYSICAL REVIEW E, 77(3), 31912. https://doi.org/10.1103/PhysRevE.77.031912
Fuhrmann, Alexander, Anselmetti, Dario, Ros, Robert, Getfert, Sebastian, and Reimann, Peter. 2008. “Refined procedure of evaluating experimental single-molecule force spectroscopy data”. PHYSICAL REVIEW E 77 (3): 31912.
Fuhrmann, A., Anselmetti, D., Ros, R., Getfert, S., and Reimann, P. (2008). Refined procedure of evaluating experimental single-molecule force spectroscopy data. PHYSICAL REVIEW E 77, 31912.
Fuhrmann, A., et al., 2008. Refined procedure of evaluating experimental single-molecule force spectroscopy data. PHYSICAL REVIEW E, 77(3), p 31912.
A. Fuhrmann, et al., “Refined procedure of evaluating experimental single-molecule force spectroscopy data”, PHYSICAL REVIEW E, vol. 77, 2008, pp. 31912.
Fuhrmann, A., Anselmetti, D., Ros, R., Getfert, S., Reimann, P.: Refined procedure of evaluating experimental single-molecule force spectroscopy data. PHYSICAL REVIEW E. 77, 31912 (2008).
Fuhrmann, Alexander, Anselmetti, Dario, Ros, Robert, Getfert, Sebastian, and Reimann, Peter. “Refined procedure of evaluating experimental single-molecule force spectroscopy data”. PHYSICAL REVIEW E 77.3 (2008): 31912.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
6 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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