Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes

Shen Y, Betzendahl I, Tinneberg H-R, Eichenlaub-Ritter U (2008)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Shen, Ying; Betzendahl, Ilse; Tinneberg, Hans-Rudolf; Eichenlaub-Ritter, UrsulaUniBi
Abstract / Bemerkung
Chromosomal non-disjunction in female meiosis gives rise to reduced fertility and trisomy in humans. Human oocytes, especially from aged women, appear especially susceptible to non-disjunction. The oocyte spindle is crucial for high fidelity of chromosome segregation at meiotic divisions, and alterations in spindle morphology are therefore indicators of adverse conditions during oocyte development that may result in meiotic aneuploidy. In the past, oocytes had to be fixed for spindle analysis, precluding direct non-invasive identification of aneugens and adverse maturation conditions that affect spindle integrity and chromosome behaviour. Aneuploidy research for detection of spindle aberrations was therefore mainly focused on in vivo or in vitro exposed, fixed animal oocytes or cytogenetic analysis of spread oocytes. Orientation independent enhanced polarizing microscopy with nearly circularly polarized light and electronically controlled liquid crystal compensator optics is a new tool to study spindle morphology non-invasively in vivo for qualitative as well as quantitative analysis. Image generation by polarization microscopy depends on the intrinsic optical properties of the spindle with its paracrystalline microtubule lattice. When polarized light passes through such a lattice it induces a splitting of the beam and shift in the plane of vibration and retardation of light (termed birefringence and retardance). Studies of animal oocytes and follicle-cell denuded human oocytes fertilized by intracytoplasmic sperm injection for assisted conception have demonstrated the safety and efficacy of enhanced polarization microscopy. The method can be employed in aneuploidy research for non-invasive dose-response studies to detect spindle aberrations, for instance, in combination with cytogenetic analysis. Due to the non-invasive nature of the technique it may be employed in routine analysis of human oocytes to assess risks by lifestyle factors, and occupational and adverse environmental exposures. (c) 2007 Elsevier B.V. All rights reserved.
spindle; maturation; aneuploidy; polarizing microscopy; oocyte
Page URI


Shen Y, Betzendahl I, Tinneberg H-R, Eichenlaub-Ritter U. Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. 2008;651(1-2):131-140.
Shen, Y., Betzendahl, I., Tinneberg, H. - R., & Eichenlaub-Ritter, U. (2008). Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 651(1-2), 131-140. https://doi.org/10.1016/j.mrgentox.2007.10.013
Shen, Y., Betzendahl, I., Tinneberg, H. - R., and Eichenlaub-Ritter, U. (2008). Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 651, 131-140.
Shen, Y., et al., 2008. Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 651(1-2), p 131-140.
Y. Shen, et al., “Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes”, MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, vol. 651, 2008, pp. 131-140.
Shen, Y., Betzendahl, I., Tinneberg, H.-R., Eichenlaub-Ritter, U.: Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. 651, 131-140 (2008).
Shen, Ying, Betzendahl, Ilse, Tinneberg, Hans-Rudolf, and Eichenlaub-Ritter, Ursula. “Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes”. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 651.1-2 (2008): 131-140.

23 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Regulation of the meiotic divisions of mammalian oocytes and eggs.
Sanders JR, Jones KT., Biochem Soc Trans 46(4), 2018
PMID: 29934303
Predictive value of spindle retardance in embryo implantation rate.
García-Oro S, Rey MI, Rodríguez M, Durán Á, Devesa R, Valverde D., J Assist Reprod Genet 34(5), 2017
PMID: 28281144
Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos.
Karnowski K, Ajduk A, Wieloch B, Tamborski S, Krawiec K, Wojtkowski M, Szkulmowski M., Sci Rep 7(1), 2017
PMID: 28646146
The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte.
Mihalas BP, De Iuliis GN, Redgrove KA, McLaughlin EA, Nixon B., Sci Rep 7(1), 2017
PMID: 28740075
Potential technique for tiny crystalline detection in lycopene-loaded SLN and NLC development.
Okonogi S, Riangjanapatee P., Drug Dev Ind Pharm 40(10), 2014
PMID: 23937584
Developmental competence of equine oocytes: impacts of zona pellucida birefringence and maternally derived transcript expression.
Mohammadi-Sangcheshmeh A, Held E, Rings F, Ghanem N, Salilew-Wondim D, Tesfaye D, Sieme H, Schellander K, Hoelker M., Reprod Fertil Dev 26(3), 2014
PMID: 23622680
Assessment of meiotic spindle configuration and post-warming bovine oocyte viability using polarized light microscopy.
Caamaño JN, Díez C, Trigal B, Muñoz M, Morató R, Martín D, Carrocera S, Mogas T, Gómez E., Reprod Domest Anim 48(3), 2013
PMID: 23106568
Quality control of embryo development.
Ajduk A, Zernicka-Goetz M., Mol Aspects Med 34(5), 2013
PMID: 23563243
Zona pellucida birefringence correlates with developmental capacity of bovine oocytes classified by maturational environment, COC morphology and G6PDH activity.
Held E, Mertens EM, Mohammadi-Sangcheshmeh A, Salilew-Wondim D, Besenfelder U, Havlicek V, Herrler A, Tesfaye D, Schellander K, Hölker M., Reprod Fertil Dev 24(4), 2012
PMID: 22541545
Gamete competence assessment by polarizing optics in assisted reproduction.
Montag M, Köster M, van der Ven K, van der Ven H., Hum Reprod Update 17(5), 2011
PMID: 21531752
Comparative analysis of the metaphase II spindle of human oocytes through polarized light and high-performance confocal microscopy.
Coticchio G, Sciajno R, Hutt K, Bromfield J, Borini A, Albertini DF., Fertil Steril 93(6), 2010
PMID: 19243751
Analysis of spindle characteristics and embryo quality in mice stimulated with letrozole using Polscope imaging.
Ganesh A, Chattopadhyay R, Narendra Babu K, Chakravarty B, Chaudhury K, Chaudhury K., Fertil Steril 93(5), 2010
PMID: 19646690
MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes.
Vogt E, Sanhaji M, Klein W, Seidel T, Wordeman L, Eichenlaub-Ritter U., Mol Hum Reprod 16(9), 2010
PMID: 20406800
Polarized light microscopy in mammalian oocytes.
Caamaño JN, Muñoz M, Diez C, Gómez E., Reprod Domest Anim 45 Suppl 2(), 2010
PMID: 20591065

99 References

Daten bereitgestellt von Europe PubMed Central.

The chromosome complement of human gametes.
Jacobs PA., Oxf Rev Reprod Biol 14(), 1992
PMID: 1437215
To err (meiotically) is human: the genesis of human aneuploidy.
Hassold T, Hunt P., Nat. Rev. Genet. 2(4), 2001
PMID: 11283700
Cytogenetic analyses of culture failures by comparative genomic hybridisation (CGH)-Re-evaluation of chromosome aberration rates in early spontaneous abortions.
Fritz B, Hallermann C, Olert J, Fuchs B, Bruns M, Aslan M, Schmidt S, Coerdt W, Muntefering H, Rehder H., Eur. J. Hum. Genet. 9(7), 2001
PMID: 11464246
Aneuploidy in aging oocytes and after oxic insult
Eichenlaub-Ritter, 2003
Differences in chromosome susceptibility to aneuploidy and survival to first trimester.
Munne S, Bahce M, Sandalinas M, Escudero T, Marquez C, Velilla E, Colls P, Oter M, Alikani M, Cohen J., Reprod. Biomed. Online 8(1), 2004
PMID: 14759293
FISH screening of aneuploidies in preimplantation embryos to improve IVF outcome.
Rubio C, Rodrigo L, Perez-Cano I, Mercader A, Mateu E, Buendia P, Remohi J, Simon C, Pellicer A., Reprod. Biomed. Online 11(4), 2005
PMID: 16274616
Chromosome malsegregation and embryonic lethality induced by treatment of normally ovulated mouse oocytes with nocodazole.
Generoso WM, Katoh M, Cain KT, Hughes LA, Foxworth LB, Mitchell TJ, Bishop JB., Mutat. Res. 210(2), 1989
PMID: 2911257
Differential sensitivity of mouse oocytes to colchicine-induced aneuploidy.
Mailhes JB, Yuan ZP., Environ. Mol. Mutagen. 10(2), 1987
PMID: 3691490
Taxol-induced meiotic maturation delay, spindle defects, and aneuploidy in mouse oocytes and zygotes.
Mailhes JB, Carabatsos MJ, Young D, London SN, Bell M, Albertini DF., Mutat. Res. 423(1-2), 1999
PMID: 10029682
Meiotic arrest and aneuploidy induced by vinblastine in mouse oocytes.
Russo A, Pacchierotti F., Mutat. Res. 202(1), 1988
PMID: 3185591
Aneuploidy in mouse metaphase II oocytes exposed in vivo and in vitro in preantral follicle culture to nocodazole.
Sun F, Betzendahl I, Pacchierotti F, Ranaldi R, Smitz J, Cortvrindt R, Eichenlaub-Ritter U., Mutagenesis 20(1), 2005
PMID: 15701686
Non-invasive method to assess genotoxicity of nocodazole interfering with spindle formation in mammalian oocytes.
Shen Y, Betzendahl I, Sun F, Tinneberg HR, Eichenlaub-Ritter U., Reprod. Toxicol. 19(4), 2005
PMID: 15749259
Mechanisms and chemical induction of aneuploidy in rodent germ cells.
Mailhes JB, Marchetti F., Cytogenet. Genome Res. 111(3-4), 2005
PMID: 16192721
Mechanisms and risk of chemically induced aneuploidy in mammalian germ cells.
Pacchierotti F, Ranaldi R., Curr. Pharm. Des. 12(12), 2006
PMID: 16611130
Maternal aging and chromosomal abnormalities: new data drawn from in vitro unfertilized human oocytes.
Pellestor F, Andreo B, Arnal F, Humeau C, Demaille J., Hum. Genet. 112(2), 2002
PMID: 12522562
Frequency and distribution of chromosome abnormalities in human oocytes.
Kuliev A, Cieslak J, Verlinsky Y., Cytogenet. Genome Res. 111(3-4), 2005
PMID: 16192694
Mechanisms and targets involved in maternal and paternal age effects on numerical aneuploidy.
Wyrobek AJ, Aardema M, Eichenlaub-Ritter U, Ferguson L, Marchetti F., Environ. Mol. Mutagen. 28(3), 1996
PMID: 8908184
The microtubular cytoskeleton and chromosomes of unfertilized human oocytes aged in vitro.
Eichenlaub-Ritter U, Stahl A, Luciani JM., Hum. Genet. 80(3), 1988
PMID: 3192214
SMC1beta-deficient female mice provide evidence that cohesins are a missing link in age-related nondisjunction.
Hodges CA, Revenkova E, Jessberger R, Hassold TJ, Hunt PA., Nat. Genet. 37(12), 2005
PMID: 16258540
Association between spindle assembly checkpoint expression and maternal age in human oocytes.
Steuerwald N, Cohen J, Herrera RJ, Sandalinas M, Brenner CA., Mol. Hum. Reprod. 7(1), 2001
PMID: 11134360
BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice.
Baker DJ, Jeganathan KB, Cameron JD, Thompson M, Juneja S, Kopecka A, Kumar R, Jenkins RB, de Groen PC, Roche P, van Deursen JM., Nat. Genet. 36(7), 2004
PMID: 15208629
Genetics of oocyte ageing.
Eichenlaub-Ritter U., Maturitas 30(2), 1998
PMID: 9871909
Maternal age and trisomy--a unifying mechanism of formation.
Wolstenholme J, Angell RR., Chromosoma 109(7), 2000
PMID: 11151672
A molecular model for sporadic human aneuploidy.
Warren WD, Gorringe KL., Trends Genet. 22(4), 2006
PMID: 16497408
Manipulation of the oocyte: possible damage to the spindle apparatus.
Eichenlaub-Ritter U, Shen Y, Tinneberg HR., Reprod. Biomed. Online 5(2), 2002
PMID: 12419035
Chloral hydrate induced spindle aberrations, metaphase I arrest and aneuploidy in mouse oocytes.
Eichenlaub-Ritter U, Betzendahl I., Mutagenesis 10(6), 1995
PMID: 8596466
Demonstration of a mechanism of aneuploidy in human oocytes using Multifluor fluorescence in situ hybridization.
Clyde JM, Gosden RG, Rutherford AJ, Picton HM., Fertil. Steril. 76(4), 2001
PMID: 11591425
Diazepam induces meiotic delay, aneuploidy and predivision of homologues and chromatids in mammalian oocytes.
Yin H, Baart E, Betzendahl I, Eichenlaub-Ritter U., Mutagenesis 13(6), 1998
PMID: 9862187
Trichlorfon exposure, spindle aberrations and nondisjunction in mammalian oocytes.
Yin H, Cukurcam S, Betzendahl I, Adler ID, Eichenlaub-Ritter U., Chromosoma 107(6-7), 1998
PMID: 9914385
The oxidizing agent tertiary butyl hydroperoxide induces disturbances in spindle organization, c-meiosis, and aneuploidy in mouse oocytes.
Tarin JJ, Vendrell FJ, Ten J, Blanes R, van Blerkom J, Cano A., Mol. Hum. Reprod. 2(12), 1996
PMID: 9237232
Transient exposure to the Eg5 kinesin inhibitor monastrol leads to syntelic orientation of chromosomes and aneuploidy in mouse oocytes
Mailhes, Mutat. Res. 559(1/2), 2004
Model for aging: knockdown of Mad2 expression predisposes to non-disjunction in mammalian oocytes possessing aberrant spindles
Eichenlaub-Ritter, Hum. Reprod. 20(Suppl. 1), 2005
Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytes.
Homer HA, McDougall A, Levasseur M, Yallop K, Murdoch AP, Herbert M., Genes Dev. 19(2), 2005
PMID: 15655110
Effect of meiotic recombination on the production of aneuploid gametes in humans.
Lamb NE, Sherman SL, Hassold TJ., Cytogenet. Genome Res. 111(3-4), 2005
PMID: 16192701
Mouse genetic models for aneuploidy induction in germ cells.
Eichenlaub-Ritter U., Cytogenet. Genome Res. 111(3-4), 2005
PMID: 16192722
A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity.
Walczak CE, Vernos I, Mitchison TJ, Karsenti E, Heald R., Curr. Biol. 8(16), 1998
PMID: 9707401
Mos is required for MAP kinase activation and is involved in microtubule organization during meiotic maturation in the mouse.
Verlhac MH, Kubiak JZ, Weber M, Geraud G, Colledge WH, Evans MJ, Maro B., Development 122(3), 1996
PMID: 8631259
Trichlorfon predisposes to aneuploidy and interferes with spindle formation in in vitro maturing mouse oocytes.
Cukurcam S, Sun F, Betzendahl I, Adler ID, Eichenlaub-Ritter U., Mutat. Res. 564(2), 2004
PMID: 15507381
Experimental evidence that changes in oocyte growth influence meiotic chromosome segregation.
Hodges CA, Ilagan A, Jennings D, Keri R, Nilson J, Hunt PA., Hum. Reprod. 17(5), 2002
PMID: 11980735
Indirect mechanisms of genotoxicity.
Kirsch-Volders M, Vanhauwaert A, Eichenlaub-Ritter U, Decordier I., Toxicol. Lett. 140-141(), 2003
PMID: 12676452
Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women.
Battaglia DE, Goodwin P, Klein NA, Soules MR., Hum. Reprod. 11(10), 1996
PMID: 8943533
Smoking and reproduction: gene damage to human gametes and embryos.
Zenzes MT., Hum. Reprod. Update 6(2), 2000
PMID: 10782570
The occurrence of aneuploidy in human: lessons from the cytogenetic studies of human oocytes.
Pellestor F, Andreo B, Anahory T, Hamamah S., Eur J Med Genet 49(2), 2005
PMID: 16530707
Polarized light microscopy of spindles.
Oldenbourg R., Methods Cell Biol. 61(), 1999
PMID: 9891315
Image sharpness and contrast transfer in coherent confocal microscopy.
Oldenbourg R, Terada H, Tiberio R, Inoue S., J Microsc 172(Pt 1), 1993
PMID: 8289226

Analysis of edge birefringence.
Oldenbourg R., Biophys. J. 60(3), 1991
PMID: 1932552
Muscle fine structure and microtubule birefringence measured with a new pol-scope.
Tran PT, Inoue S, Salmon ED, Oldenbourg R., Biol. Bull. 187(2), 1994
PMID: 7811800
Quantifying single and bundled microtubules with the polarized light microscope.
Tran P, Salmon ED, Oldenbourg R., Biol. Bull. 189(2), 1995
PMID: 8541402
High magnitude of light retardation by the zona pellucida is associated with conception cycles.
Shen Y, Stalf T, Mehnert C, Eichenlaub-Ritter U, Tinneberg HR., Hum. Reprod. 20(6), 2005
PMID: 15734754
Light retardance by human oocyte spindle is positively related to pronuclear score after ICSI.
Shen Y, Stalf T, Mehnert C, De Santis L, Cino I, Tinneberg HR, Eichenlaub-Ritter U., Reprod. Biomed. Online 12(6), 2006
PMID: 16792851
A reliable, noninvasive technique for spindle imaging and enucleation of mammalian oocytes.
Liu L, Oldenbourg R, Trimarchi JR, Keefe DL., Nat. Biotechnol. 18(2), 2000
PMID: 10657133
The first polar body does not predict accurately the location of the metaphase II meiotic spindle in mammalian oocytes.
Silva CP, Kommineni K, Oldenbourg R, Keefe DL., Fertil. Steril. 71(4), 1999
PMID: 10202885
2-methoxyestradiol induces spindle aberrations, chromosome congression failure, and nondisjunction in mouse oocytes.
Eichenlaub-Ritter U, Winterscheidt U, Vogt E, Shen Y, Tinneberg HR, Sorensen R., Biol. Reprod. 76(5), 2007
PMID: 17229934
Arsenite induces aberrations in meiosis that can be prevented by coadministration of N-acetylcysteine in mice.
Navarro PA, Liu L, Ferriani RA, Keefe DL., Fertil. Steril. 85 Suppl 1(), 2006
PMID: 16616091
The telomere theory of reproductive senescence in women.
Keefe DL, Marquard K, Liu L., Curr. Opin. Obstet. Gynecol. 18(3), 2006
PMID: 16735827
The spindle observation and its relationship with fertilization after intracytoplasmic sperm injection in living human oocytes.
Wang WH, Meng L, Hackett RJ, Odenbourg R, Keefe DL., Fertil. Steril. 75(2), 2001
PMID: 11172838
Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures.
Rienzi L, Martinez F, Ubaldi F, Minasi MG, Iacobelli M, Tesarik J, Greco E., Hum. Reprod. 19(3), 2004
PMID: 14998966
Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI.
Rienzi L, Ubaldi F, Martinez F, Iacobelli M, Minasi MG, Ferrero S, Tesarik J, Greco E., Hum. Reprod. 18(6), 2003
PMID: 12773461
Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro.
Li Y, Feng HL, Cao YJ, Zheng GJ, Yang Y, Mullen S, Critser JK, Chen ZJ., Fertil. Steril. 85(4), 2006
PMID: 16580356


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 18160331
PubMed | Europe PMC

Suchen in

Google Scholar