Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen

Krassen H, Stripp S, von Abendroth G, Ataka K, Happe T, Heberle J (2009)
JOURNAL OF BIOTECHNOLOGY 142(1): 3-9.

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Abstract / Bemerkung
Hydrogenase-modified electrodes are a promising catalytic surface for the electrolysis of water with an overpotential close to zero. The [FeFe]-hydrogenase CrHydA1 from the photosynthetic green alga Chlamydomonas reinhardtii is the smallest [ FeFe]-hydrogenase known and exhibits an extraordinary high hydrogen evolution activity. For the first time, we immobilized CrHydA1 on a gold surface which was modified by different carboxy-terminated self-assembled monolayers. The immobilization was in situ monitored by surface-enhanced infrared spectroscopy. In the presence of the electron mediator methyl viologen the electron transfer from the electrode to the hydrogenase was detected by cyclic voltammetry. The hydrogen evolution potential (-290 mV vs NHE, pH 6.8) of this protein modified electrode is close to the value for bare platinum (-270 mV vs NHE). The surface coverage by CrHydA1 was determined to 2.25 ng mm(-2) by surface plasmon resonance, which is consistent with the formation of a protein monolayer. Hydrogen evolution was quantified by gas chromatography and the specific hydrogen evolution activity of surface-bound CrHydA1 was calculated to 1.3 mu mol H-2 min(-1) mg(-1) (or 85 mol H-2 min(-1) mol(-1)). In conclusion, a viable hydrogen-evolving surface was developed that may be employed in combination with immobilized photosystems to provide a platform for hydrogen production from water and solar energy with enzymes as catalysts. (C) 2009 Elsevier B.V. All rights reserved.
Stichworte
Electron transfer; Enzyme-modified electrode; Monolayer; FTIR; Biological hydrogen production; [FeFe]-hydrogenase; SEIRA
Erscheinungsjahr
2009
Band
142
Ausgabe
1
Seite(n)
3-9
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1591836

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Krassen H, Stripp S, von Abendroth G, Ataka K, Happe T, Heberle J. Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. JOURNAL OF BIOTECHNOLOGY. 2009;142(1):3-9.
Krassen, H., Stripp, S., von Abendroth, G., Ataka, K., Happe, T., & Heberle, J. (2009). Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. JOURNAL OF BIOTECHNOLOGY, 142(1), 3-9. doi:10.1016/j.jbiotec.2009.01.018
Krassen, H., Stripp, S., von Abendroth, G., Ataka, K., Happe, T., and Heberle, J. (2009). Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. JOURNAL OF BIOTECHNOLOGY 142, 3-9.
Krassen, H., et al., 2009. Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. JOURNAL OF BIOTECHNOLOGY, 142(1), p 3-9.
H. Krassen, et al., “Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen”, JOURNAL OF BIOTECHNOLOGY, vol. 142, 2009, pp. 3-9.
Krassen, H., Stripp, S., von Abendroth, G., Ataka, K., Happe, T., Heberle, J.: Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. JOURNAL OF BIOTECHNOLOGY. 142, 3-9 (2009).
Krassen, Henning, Stripp, Sven, von Abendroth, Gregory, Ataka, Kenichi, Happe, Thomas, and Heberle, Joachim. “Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen”. JOURNAL OF BIOTECHNOLOGY 142.1 (2009): 3-9.

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