Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen

Krassen H, Stripp S, von Abendroth G, Ataka K, Happe T, Heberle J (2009)
In: Journal of Biotechnology. JOURNAL OF BIOTECHNOLOGY, 142(1). ELSEVIER SCIENCE BV: 3-9.

Konferenzbeitrag | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Krassen, Henning; Stripp, Sven; von Abendroth, Gregory; Ataka, Kenichi; Happe, Thomas; Heberle, Joachim
Abstract / Bemerkung
Hydrogenase-modified electrodes are a promising catalytic surface for the electrolysis of water with an overpotential close to zero. The [FeFe]-hydrogenase CrHydA1 from the photosynthetic green alga Chlamydomonas reinhardtii is the smallest [ FeFe]-hydrogenase known and exhibits an extraordinary high hydrogen evolution activity. For the first time, we immobilized CrHydA1 on a gold surface which was modified by different carboxy-terminated self-assembled monolayers. The immobilization was in situ monitored by surface-enhanced infrared spectroscopy. In the presence of the electron mediator methyl viologen the electron transfer from the electrode to the hydrogenase was detected by cyclic voltammetry. The hydrogen evolution potential (-290 mV vs NHE, pH 6.8) of this protein modified electrode is close to the value for bare platinum (-270 mV vs NHE). The surface coverage by CrHydA1 was determined to 2.25 ng mm(-2) by surface plasmon resonance, which is consistent with the formation of a protein monolayer. Hydrogen evolution was quantified by gas chromatography and the specific hydrogen evolution activity of surface-bound CrHydA1 was calculated to 1.3 mu mol H-2 min(-1) mg(-1) (or 85 mol H-2 min(-1) mol(-1)). In conclusion, a viable hydrogen-evolving surface was developed that may be employed in combination with immobilized photosystems to provide a platform for hydrogen production from water and solar energy with enzymes as catalysts. (C) 2009 Elsevier B.V. All rights reserved.
Stichworte
Electron transfer; Enzyme-modified electrode; Monolayer; FTIR; Biological hydrogen production; [FeFe]-hydrogenase; SEIRA
Erscheinungsjahr
2009
Titel des Konferenzbandes
Journal of Biotechnology
Band
142
Ausgabe
1
Seite(n)
3-9
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1591836

Zitieren

Krassen H, Stripp S, von Abendroth G, Ataka K, Happe T, Heberle J. Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. In: Journal of Biotechnology. JOURNAL OF BIOTECHNOLOGY. Vol 142. ELSEVIER SCIENCE BV; 2009: 3-9.
Krassen, H., Stripp, S., von Abendroth, G., Ataka, K., Happe, T., & Heberle, J. (2009). Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. Journal of Biotechnology, JOURNAL OF BIOTECHNOLOGY, 142, 3-9. ELSEVIER SCIENCE BV. doi:10.1016/j.jbiotec.2009.01.018
Krassen, H., Stripp, S., von Abendroth, G., Ataka, K., Happe, T., and Heberle, J. (2009). “Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen” in Journal of Biotechnology JOURNAL OF BIOTECHNOLOGY, vol. 142, (ELSEVIER SCIENCE BV), 3-9.
Krassen, H., et al., 2009. Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. In Journal of Biotechnology. JOURNAL OF BIOTECHNOLOGY. no.142 ELSEVIER SCIENCE BV, pp. 3-9.
H. Krassen, et al., “Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen”, Journal of Biotechnology, JOURNAL OF BIOTECHNOLOGY, vol. 142, ELSEVIER SCIENCE BV, 2009, pp.3-9.
Krassen, H., Stripp, S., von Abendroth, G., Ataka, K., Happe, T., Heberle, J.: Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen. Journal of Biotechnology. JOURNAL OF BIOTECHNOLOGY. 142, p. 3-9. ELSEVIER SCIENCE BV (2009).
Krassen, Henning, Stripp, Sven, von Abendroth, Gregory, Ataka, Kenichi, Happe, Thomas, and Heberle, Joachim. “Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen”. Journal of Biotechnology. ELSEVIER SCIENCE BV, 2009.Vol. 142. JOURNAL OF BIOTECHNOLOGY. 3-9.

9 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Methodologies for "Wiring" Redox Proteins/Enzymes to Electrode Surfaces.
Yates NDJ, Fascione MA, Parkin A., Chemistry 24(47), 2018
PMID: 29637638
Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases.
Duan J, Senger M, Esselborn J, Engelbrecht V, Wittkamp F, Apfel UP, Hofmann E, Stripp ST, Happe T, Winkler M., Nat Commun 9(1), 2018
PMID: 30413719
Analyzing the catalytic processes of immobilized redox enzymes by vibrational spectroscopies.
Sezer M, Millo D, Weidinger IM, Zebger I, Hildebrandt P., IUBMB Life 64(6), 2012
PMID: 22535701
In vitro hydrogen production--using energy from the sun.
Krassen H, Ott S, Heberle J., Phys Chem Chem Phys 13(1), 2011
PMID: 21103567
Microalgal hydrogen production.
Kruse O, Hankamer B., Curr Opin Biotechnol 21(3), 2010
PMID: 20399635
Thinner, smaller, faster: IR techniques to probe the functionality of biological and biomimetic systems.
Ataka K, Kottke T, Heberle J., Angew Chem Int Ed Engl 49(32), 2010
PMID: 20818765
How algae produce hydrogen--news from the photosynthetic hydrogenase.
Stripp ST, Happe T., Dalton Trans (45), 2009
PMID: 19904421

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 19480942
PubMed | Europe PMC

Suchen in

Google Scholar