Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules

Doose S, Neuweiler H, Sauer M (2009)
ChemPhysChem 10(9-10): 1389-1398.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Doose, Sören; Neuweiler, Hannes; Sauer, MarkusUniBi
Abstract / Bemerkung
Photoinduced electron transfer (PET) between organic fluorophores and suitable electron donating moieties, for example, the amino acid tryptophan or the nucleobase guanine, can quench fluorescence upon van der Waals contact and thus report-on molecular contact. PET-quenching has been used as reporter for monitoring conformational dynamics in polypeptides, proteins, and oligonucleotides. Whereas dynamic quenching transiently influences quantum yield and fluorescence life-time of the fluorophore, static quenching in pi-stacked complexes efficiently suppresses fluorescence emission over time scales longer than the fluorescence lifetime. Static quenching therefore provides sufficient contrast to be observed at the single-molecule level. Here, we review complex formation and static quenching of different fluorophores by various molecular compounds, discuss applications as reporter system for macromolecular dynamics, and give illustrating examples.
molecular diagnostics; fluorescence spectroscopy; biopolymer conformational dynamics; photoinduced electron transfer; protein folding
Page URI


Doose S, Neuweiler H, Sauer M. Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules. ChemPhysChem. 2009;10(9-10):1389-1398.
Doose, S., Neuweiler, H., & Sauer, M. (2009). Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules. ChemPhysChem, 10(9-10), 1389-1398. https://doi.org/10.1002/cphc.200900238
Doose, Sören, Neuweiler, Hannes, and Sauer, Markus. 2009. “Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules”. ChemPhysChem 10 (9-10): 1389-1398.
Doose, S., Neuweiler, H., and Sauer, M. (2009). Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules. ChemPhysChem 10, 1389-1398.
Doose, S., Neuweiler, H., & Sauer, M., 2009. Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules. ChemPhysChem, 10(9-10), p 1389-1398.
S. Doose, H. Neuweiler, and M. Sauer, “Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules”, ChemPhysChem, vol. 10, 2009, pp. 1389-1398.
Doose, S., Neuweiler, H., Sauer, M.: Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules. ChemPhysChem. 10, 1389-1398 (2009).
Doose, Sören, Neuweiler, Hannes, and Sauer, Markus. “Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules”. ChemPhysChem 10.9-10 (2009): 1389-1398.

136 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

A Conditionally Fluorescent Peptide Reporter of Secondary Structure Modulation.
Johnson OT, Kaur T, Garner AL., Chembiochem 20(1), 2019
PMID: 30137694
Single-molecule photoredox catalysis.
Haimerl J, Ghosh I, König B, Vogelsang J, Lupton JM., Chem Sci 10(3), 2019
PMID: 30746104
Excited-State Dynamics of [Ru(bpy)3 ]2+ Thin Films on Sensitized TiO2 and ZrO2.
Leandri V, Liu P, Sadollahkhani A, Safdari M, Kloo L, Gardner JM., Chemphyschem 20(4), 2019
PMID: 30623544
Probing G-quadruplex topologies and recognition concurrently in real time and 3D using a dual-app nucleoside probe.
Nuthanakanti A, Ahmed I, Khatik SY, Saikrishnan K, Srivatsan SG., Nucleic Acids Res 47(12), 2019
PMID: 31106340
Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy.
Beliu G, Kurz AJ, Kuhlemann AC, Behringer-Pliess L, Meub M, Wolf N, Seibel J, Shi ZD, Schnermann M, Grimm JB, Lavis LD, Doose S, Sauer M., Commun Biol 2(), 2019
PMID: 31341960
Fluorescence Correlation Spectroscopy of Labeled Azurin Reveals Photoinduced Electron Transfer between Label and Cu Center.
Andreoni A, Sen S, Hagedoorn PL, Buma WJ, Aartsma TJ, Canters GW., Chemistry 24(3), 2018
PMID: 29064125
Fluorescence Redox Blinking Adaptable to Structural Analysis of Nucleic Acids.
Miyata T, Shimada N, Maruyama A, Kawai K., Chemistry 24(26), 2018
PMID: 29341353
A Lucifer-Based Environment-Sensitive Fluorescent PNA Probe for Imaging Poly(A) RNAs.
Sabale PM, Ambi UB, Srivatsan SG., Chembiochem 19(8), 2018
PMID: 29396904
Advances in the cellular structural biology of nucleic acids.
Giassa IC, Rynes J, Fessl T, Foldynova-Trantirkova S, Trantirek L., FEBS Lett 592(12), 2018
PMID: 29679394
Post-transcriptional labeling by using Suzuki-Miyaura cross-coupling generates functional RNA probes.
Walunj MB, Tanpure AA, Srivatsan SG., Nucleic Acids Res 46(11), 2018
PMID: 29546376
Structure of a mitochondrial fission dynamin in the closed conformation.
Bohuszewicz O, Low HH., Nat Struct Mol Biol 25(8), 2018
PMID: 30061604
Fluorescence-Lifetime-Sensitive Probes for Monitoring ATP Cleavage.
Hammler D, Marx A, Zumbusch A., Chemistry 24(57), 2018
PMID: 30070405
Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances.
Pantazis A, Westerberg K, Althoff T, Abramson J, Olcese R., Nat Commun 9(1), 2018
PMID: 30413716
Activatable fluorescence: From small molecule to nanoparticle.
Luby BM, Charron DM, MacLaughlin CM, Zheng G., Adv Drug Deliv Rev 113(), 2017
PMID: 27593264
Co-translational protein folding: progress and methods.
Thommen M, Holtkamp W, Rodnina MV., Curr Opin Struct Biol 42(), 2017
PMID: 27940242
Epicocconone-Hemicyanine Hybrids: Near Infrared Fluorophores for Protein Staining and Cell Imaging.
Karuso P, Loa Kum Cheung W, Peixoto PA, Boulangé A, Franck X., Chemistry 23(8), 2017
PMID: 27859805
Molecular Determinants of BK Channel Functional Diversity and Functioning.
Latorre R, Castillo K, Carrasquel-Ursulaez W, Sepulveda RV, Gonzalez-Nilo F, Gonzalez C, Alvarez O., Physiol Rev 97(1), 2017
PMID: 27807200
Photoinduced electron transfer in supramolecular ruthenium-porphyrin assemblies.
Rota Martir D, Averardi M, Escudero D, Jacquemin D, Zysman-Colman E., Dalton Trans 46(7), 2017
PMID: 28128833
Structure of the Ribosomal RNA Decoding Site Containing a Selenium-Modified Responsive Fluorescent Ribonucleoside Probe.
Nuthanakanti A, Boerneke MA, Hermann T, Srivatsan SG., Angew Chem Int Ed Engl 56(10), 2017
PMID: 28156044
Integrated view of internal friction in unfolded proteins from single-molecule FRET, contact quenching, theory, and simulations.
Soranno A, Holla A, Dingfelder F, Nettels D, Makarov DE, Schuler B., Proc Natl Acad Sci U S A 114(10), 2017
PMID: 28223518
Antigen-responsive molecular sensor enables real-time tumor-specific imaging.
Kim H, Choi HS, Kim SK, Lee BI, Choi Y., Theranostics 7(4), 2017
PMID: 28382167
Engineering a switch-based biosensor for arginine using a Thermotoga maritima periplasmic binding protein.
Donaldson T, Iozzino L, Deacon LJ, Billones H, Ausili A, D'Auria S, Dattelbaum JD., Anal Biochem 525(), 2017
PMID: 28259516
DNA-Endonuclease Complex Dynamics by Simultaneous FRET and Fluorophore Intensity in Evanescent Field.
Tutkus M, Marciulionis T, Sasnauskas G, Rutkauskas D., Biophys J 112(5), 2017
PMID: 28297644
Caging and Photoactivation in Single-Molecule Förster Resonance Energy Transfer Experiments.
Jazi AA, Ploetz E, Arizki M, Dhandayuthapani B, Waclawska I, Krämer R, Ziegler C, Cordes T., Biochemistry 56(14), 2017
PMID: 28362086
Activation pH and Gating Dynamics of Influenza A M2 Proton Channel Revealed by Single-Molecule Spectroscopy.
Lin CW, Mensa B, Barniol-Xicota M, DeGrado WF, Gai F., Angew Chem Int Ed Engl 56(19), 2017
PMID: 28374543
Photoluminescence responses of graphene quantum dots toward organic bases and an acid.
Suzuki K, Yamato K, Sekiya R, Haino T., Photochem Photobiol Sci 16(5), 2017
PMID: 28463379
Covalent dye attachment influences the dynamics and conformational properties of flexible peptides.
Luitz MP, Barth A, Crevenna AH, Bomblies R, Lamb DC, Zacharias M., PLoS One 12(5), 2017
PMID: 28542243
Palladium-Mediated Synthesis of a Near-Infrared Fluorescent K+ Sensor.
Bandara HMD, Hua Z, Zhang M, Pauff SM, Miller SC, Davie EAC, Kobertz WR., J Org Chem 82(15), 2017
PMID: 28664732
The effect of mangiferin on skin: Penetration, permeation and inhibition of ECM enzymes.
Ochocka R, Hering A, Stefanowicz-Hajduk J, Cal K, Barańska H., PLoS One 12(7), 2017
PMID: 28750062
Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction.
Jacobsen MT, Fairhead M, Fogelstrand P, Howarth M., Cell Chem Biol 24(8), 2017
PMID: 28757182
Characterization of Fluorescein Arsenical Hairpin (FlAsH) as a Probe for Single-Molecule Fluorescence Spectroscopy.
Fernandes DD, Bamrah J, Kailasam S, Gomes GW, Li Y, Wieden HJ, Gradinaru CC., Sci Rep 7(1), 2017
PMID: 29026195
Conjugated Polymer Nanoparticles for Bioimaging.
Braeken Y, Cheruku S, Ethirajan A, Maes W., Materials (Basel) 10(12), 2017
PMID: 29231908
CTAB enhancement of FRET in DNA structures.
Oh T, Takahashi T, Kim S, Heller MJ., J Biophotonics 9(1-2), 2016
PMID: 26530400
Molecular motions that shape the cardiac action potential: Insights from voltage clamp fluorometry.
Zhu W, Varga Z, Silva JR., Prog Biophys Mol Biol 120(1-3), 2016
PMID: 26724572
A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization.
van der Velde JH, Oelerich J, Huang J, Smit JH, Aminian Jazi A, Galiani S, Kolmakov K, Guoridis G, Eggeling C, Herrmann A, Roelfes G, Cordes T., Nat Commun 7(), 2016
PMID: 26751640
Single-Molecule FRET Spectroscopy and the Polymer Physics of Unfolded and Intrinsically Disordered Proteins.
Schuler B, Soranno A, Hofmann H, Nettels D., Annu Rev Biophys 45(), 2016
PMID: 27145874
Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism.
Schulze A, Beliu G, Helmerich DA, Schubert J, Pearl LH, Prodromou C, Neuweiler H., Nat Chem Biol 12(8), 2016
PMID: 27322067
Consistent View of Polypeptide Chain Expansion in Chemical Denaturants from Multiple Experimental Methods.
Borgia A, Zheng W, Buholzer K, Borgia MB, Schüler A, Hofmann H, Soranno A, Nettels D, Gast K, Grishaev A, Best RB, Schuler B., J Am Chem Soc 138(36), 2016
PMID: 27583570
Quantitative FRET studies and integrative modeling unravel the structure and dynamics of biomolecular systems.
Dimura M, Peulen TO, Hanke CA, Prakash A, Gohlke H, Seidel CA., Curr Opin Struct Biol 40(), 2016
PMID: 27939973
Exploring the self-assembly and energy transfer of dynamic supramolecular iridium-porphyrin systems.
Rota Martir D, Hedley GJ, Cordes DB, Slawin AM, Escudero D, Jacquemin D, Kosikova T, Philp D, Dawson DM, Ashbrook SE, Samuel ID, Zysman-Colman E., Dalton Trans 45(43), 2016
PMID: 27722693
A specialized molecular motion opens the Hv1 voltage-gated proton channel.
Mony L, Berger TK, Isacoff EY., Nat Struct Mol Biol 22(4), 2015
PMID: 25730777
LucY: A Versatile New Fluorescent Reporter Protein.
Auldridge ME, Cao H, Sen S, Franz LP, Bingman CA, Yennamalli RM, Phillips GN, Mead D, Steinmetz EJ., PLoS One 10(4), 2015
PMID: 25906065
Laser induced autofluorescence in the monitoring of β-mercaptoethanol mediated photo induced proton coupled electron transfer in proteins.
Manjunath S, Satish Rao BS, Satyamoorthy K, Mahato KK., Spectrochim Acta A Mol Biomol Spectrosc 149(), 2015
PMID: 25985124
MMP-2/9-Specific Activatable Lifetime Imaging Agent.
Rood MT, Raspe M, ten Hove JB, Jalink K, Velders AH, van Leeuwen FW., Sensors (Basel) 15(5), 2015
PMID: 25985157
A hemi-fission intermediate links two mechanistically distinct stages of membrane fission.
Mattila JP, Shnyrova AV, Sundborger AC, Hortelano ER, Fuhrmans M, Neumann S, Müller M, Hinshaw JE, Schmid SL, Frolov VA., Nature 524(7563), 2015
PMID: 26123023
Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain.
Lehmann M, Gottschalk B, Puchkov D, Schmieder P, Schwagerus S, Hackenberger CP, Haucke V, Schmoranzer J., Angew Chem Int Ed Engl 54(45), 2015
PMID: 26346505
Cotranslational protein folding on the ribosome monitored in real time.
Holtkamp W, Kokic G, Jäger M, Mittelstaet J, Komar AA, Rodnina MV., Science 350(6264), 2015
PMID: 26612953
Fluorescence spectroscopy of rhodopsins: insights and approaches.
Alexiev U, Farrens DL., Biochim Biophys Acta 1837(5), 2014
PMID: 24183695
Photophysical processes in single molecule organic fluorescent probes.
Stennett EM, Ciuba MA, Levitus M., Chem Soc Rev 43(4), 2014
PMID: 24141280
On the use of thioamides as fluorescence quenching probes for tracking protein folding and stability.
Petersson EJ, Goldberg JM, Wissner RF., Phys Chem Chem Phys 16(15), 2014
PMID: 24598971
Three-dimensional protein networks assembled by two-photon activation.
Gatterdam V, Ramadass R, Stoess T, Fichte MA, Wachtveitl J, Heckel A, Tampé R., Angew Chem Int Ed Engl 53(22), 2014
PMID: 24729568
Kinetics of DNA tile dimerization.
Jiang S, Yan H, Liu Y., ACS Nano 8(6), 2014
PMID: 24794259
DNA origami-based standards for quantitative fluorescence microscopy.
Schmied JJ, Raab M, Forthmann C, Pibiri E, Wünsch B, Dammeyer T, Tinnefeld P., Nat Protoc 9(6), 2014
PMID: 24833175
Fluorescence quenching of (dimethylamino)naphthalene dyes Badan and Prodan by tryptophan in cytochromes P450 and micelles.
Pospíšil P, Luxem KE, Ener M, Sýkora J, Kocábová J, Gray HB, Vlček A, Hof M., J Phys Chem B 118(34), 2014
PMID: 25079965
A starting point for fluorescence-based single-molecule measurements in biomolecular research.
Gust A, Zander A, Gietl A, Holzmeister P, Schulz S, Lalkens B, Tinnefeld P, Grohmann D., Molecules 19(10), 2014
PMID: 25271426
High-throughput development of a hybrid-type fluorescent glutamate sensor for analysis of synaptic transmission.
Takikawa K, Asanuma D, Namiki S, Sakamoto H, Ariyoshi T, Kimpara N, Hirose K., Angew Chem Int Ed Engl 53(49), 2014
PMID: 25297726
Lateral opening of the bacterial translocon on ribosome binding and signal peptide insertion.
Ge Y, Draycheva A, Bornemann T, Rodnina MV, Wintermeyer W., Nat Commun 5(), 2014
PMID: 25314960
Unravelling the conformations of di-(perylene bisimide acrylate) by combining time-resolved fluorescence-anisotropy experiments and molecular modelling.
Spreitler F, Sommer M, Hollfelder M, Thelakkat M, Gekle S, Köhler J., Phys Chem Chem Phys 16(47), 2014
PMID: 25358077
Silicon photon-counting avalanche diodes for single-molecule fluorescence spectroscopy.
Michalet X, Ingargiola A, Colyer RA, Scalia G, Weiss S, Maccagnani P, Gulinatti A, Rech I, Ghioni M., IEEE J Sel Top Quantum Electron 20(6), 2014
PMID: 25309114
Role of denatured-state properties in chaperonin action probed by single-molecule spectroscopy.
Hofmann H, Hillger F, Delley C, Hoffmann A, Pfeil SH, Nettels D, Lipman EA, Schuler B., Biophys J 107(12), 2014
PMID: 25517154
Single-molecule spectroscopy of protein folding dynamics--expanding scope and timescales.
Schuler B, Hofmann H., Curr Opin Struct Biol 23(1), 2013
PMID: 23312353
The pH probe CypHer™5E is effectively quenched by FM dyes.
Welzel O, Loy K, Tischbirek CH, Tabor A, Gmeiner P, Kornhuber J, Groemer TW., J Fluoresc 23(3), 2013
PMID: 23397486
Preparation of homogeneous samples of double-labelled protein suitable for single-molecule FRET measurements.
Lerner E, Hilzenrat G, Amir D, Tauber E, Garini Y, Haas E., Anal Bioanal Chem 405(18), 2013
PMID: 23649926
Systematic evaluation of fluorescence correlation spectroscopy data analysis on the nanosecond time scale.
Steger K, Bollmann S, Noé F, Doose S., Phys Chem Chem Phys 15(25), 2013
PMID: 23685745
A new fluorescent probe for distinguishing Zn2+ and Cd2+ with high sensitivity and selectivity.
Tan Y, Gao J, Yu J, Wang Z, Cui Y, Yang Y, Qian G., Dalton Trans 42(32), 2013
PMID: 23824098
Thioamide quenching of fluorescent probes through photoinduced electron transfer: mechanistic studies and applications.
Goldberg JM, Batjargal S, Chen BS, Petersson EJ., J Am Chem Soc 135(49), 2013
PMID: 24266520
Efficient synthesis and in vivo incorporation of acridon-2-ylalanine, a fluorescent amino acid for lifetime and Förster resonance energy transfer/luminescence resonance energy transfer studies.
Speight LC, Muthusamy AK, Goldberg JM, Warner JB, Wissner RF, Willi TS, Woodman BF, Mehl RA, Petersson EJ., J Am Chem Soc 135(50), 2013
PMID: 24303933
Label-free fluorescence detection of the depurination activity of ribosome inactivating protein toxins.
Tanpure AA, Patheja P, Srivatsan SG., Chem Commun (Camb) 48(4), 2012
PMID: 22105782
Fluorogenic protein labeling through photoinduced electron transfer-based BL-tag technology.
Sadhu KK, Mizukami S, Lanam CR, Kikuchi K., Chem Asian J 7(2), 2012
PMID: 22190536
Spectroscopic studies of protein folding: linear and nonlinear methods.
Serrano AL, Waegele MM, Gai F., Protein Sci 21(2), 2012
PMID: 22109973
Live-cell super-resolution imaging with synthetic fluorophores.
van de Linde S, Heilemann M, Sauer M., Annu Rev Phys Chem 63(), 2012
PMID: 22404589
SulA inhibits assembly of FtsZ by a simple sequestration mechanism.
Chen Y, Milam SL, Erickson HP., Biochemistry 51(14), 2012
PMID: 22432817
Minimalist probes for studying protein dynamics: thioamide quenching of selectively excitable fluorescent amino acids.
Goldberg JM, Speight LC, Fegley MW, Petersson EJ., J Am Chem Soc 134(14), 2012
PMID: 22471784
Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer.
Liu X, Li J, Dong J, Hu C, Gong W, Wang J., Angew Chem Int Ed Engl 51(41), 2012
PMID: 22936654
Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging.
van de Linde S, Krstić I, Prisner T, Doose S, Heilemann M, Sauer M., Photochem Photobiol Sci 10(4), 2011
PMID: 21152594
Distortion of tRNA upon near-cognate codon recognition on the ribosome.
Mittelstaet J, Konevega AL, Rodnina MV., J Biol Chem 286(10), 2011
PMID: 21212264
Unfolding dynamics of cytochrome c revealed by single-molecule and ensemble-averaged spectroscopy.
Choi J, Kim S, Tachikawa T, Fujitsuka M, Majima T., Phys Chem Chem Phys 13(13), 2011
PMID: 21305089
Protein folding at single-molecule resolution.
Ferreon AC, Deniz AA., Biochim Biophys Acta 1814(8), 2011
PMID: 21303706
Dynamical fingerprints for probing individual relaxation processes in biomolecular dynamics with simulations and kinetic experiments.
Noé F, Doose S, Daidone I, Löllmann M, Sauer M, Chodera JD, Smith JC., Proc Natl Acad Sci U S A 108(12), 2011
PMID: 21368203
Backbone-driven collapse in unfolded protein chains.
Teufel DP, Johnson CM, Lum JK, Neuweiler H., J Mol Biol 409(2), 2011
PMID: 21497607
Conformational changes of FtsZ reported by tryptophan mutants.
Chen Y, Erickson HP., Biochemistry 50(21), 2011
PMID: 21510681
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde S, Löschberger A, Klein T, Heidbreder M, Wolter S, Heilemann M, Sauer M., Nat Protoc 6(7), 2011
PMID: 21720313
Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions.
Bollmann S, Löllmann M, Sauer M, Doose S., Phys Chem Chem Phys 13(28), 2011
PMID: 21687885
Protonation/deprotonation effects on the stability of the Trp-cage miniprotein.
Jimenez-Cruz CA, Makhatadze GI, Garcia AE., Phys Chem Chem Phys 13(38), 2011
PMID: 21773639
Determination of copper(II) ion concentration by lifetime measurements of green fluorescent protein.
Hötzer B, Ivanov R, Altmeier S, Kappl R, Jung G., J Fluoresc 21(6), 2011
PMID: 21773693
Probing the charge-transfer dynamics in DNA at the single-molecule level.
Kawai K, Matsutani E, Maruyama A, Majima T., J Am Chem Soc 133(39), 2011
PMID: 21875061
Conformational flexibility of glycosylated peptides.
Bollmann S, Burgert A, Plattner C, Nagel L, Sewald N, Löllmann M, Sauer M, Doose S., Chemphyschem 12(16), 2011
PMID: 21922630
A fluorescence turn-on sensor for iodide based on a thymine-Hg(II)-thymine complex.
Ma B, Zeng F, Zheng F, Wu S., Chemistry 17(52), 2011
PMID: 22113734
Fluorescent probes and delivery methods for single-molecule experiments.
Lymperopoulos K, Kiel A, Seefeld A, Stöhr K, Herten DP., Chemphyschem 11(1), 2010
PMID: 19960557
Mechanisms of quenching of Alexa fluorophores by natural amino acids.
Chen H, Ahsan SS, Santiago-Berrios MB, Abruña HD, Webb WW., J Am Chem Soc 132(21), 2010
PMID: 20446733
Structure-switching biosensors: inspired by Nature.
Vallée-Bélisle A, Plaxco KW., Curr Opin Struct Biol 20(4), 2010
PMID: 20627702
Single-molecule spectroscopy of protein folding in a chaperonin cage.
Hofmann H, Hillger F, Pfeil SH, Hoffmann A, Streich D, Haenni D, Nettels D, Lipman EA, Schuler B., Proc Natl Acad Sci U S A 107(26), 2010
PMID: 20547872
Make them blink: probes for super-resolution microscopy.
Vogelsang J, Steinhauer C, Forthmann C, Stein IH, Person-Skegro B, Cordes T, Tinnefeld P., Chemphyschem 11(12), 2010
PMID: 20632356
Multicolor monitoring of dysregulated protein kinases in chronic myelogenous leukemia.
Wang Q, Zimmerman EI, Toutchkine A, Martin TD, Graves LM, Lawrence DS., ACS Chem Biol 5(9), 2010
PMID: 20583816
Direct observation of ultrafast folding and denatured state dynamics in single protein molecules.
Neuweiler H, Johnson CM, Fersht AR., Proc Natl Acad Sci U S A 106(44), 2009
PMID: 19841261

106 References

Daten bereitgestellt von Europe PubMed Central.

Dynamic personalities of proteins.
Henzler-Wildman K, Kern D., Nature 450(7172), 2007
PMID: 18075575
Function and structure of inherently disordered proteins.
Dunker AK, Silman I, Uversky VN, Sussman JL., Curr. Opin. Struct. Biol. 18(6), 2008
PMID: 18952168
Recognition dynamics up to microseconds revealed from an RDC-derived ubiquitin ensemble in solution.
Lange OF, Lakomek NA, Fares C, Schroder GF, Walter KF, Becker S, Meiler J, Grubmuller H, Griesinger C, de Groot BL., Science 320(5882), 2008
PMID: 18556554
The protein folding problem.
Dill KA, Ozkan SB, Shell MS, Weikl TR., Annu Rev Biophys 37(), 2008
PMID: 18573083
The speed limit for protein folding measured by triplet-triplet energy transfer.
Bieri O, Wirz J, Hellrung B, Schutkowski M, Drewello M, Kiefhaber T., Proc. Natl. Acad. Sci. U.S.A. 96(17), 1999
PMID: 10449738
The power and prospects of fluorescence microscopies and spectroscopies.
Michalet X, Kapanidis AN, Laurence T, Pinaud F, Doose S, Pflughoefft M, Weiss S., Annu Rev Biophys Biomol Struct 32(), 2003
PMID: 12598370

Tinnefeld, Angew. Chem. 117(), 2005

AUTHOR UNKNOWN, Angew. Chem. Int. Ed. 44(), 2005
Recent advances in FRET: distance determination in protein-DNA complexes.
Hillisch A, Lorenz M, Diekmann S., Curr. Opin. Struct. Biol. 11(2), 2001
PMID: 11297928
The renaissance of fluorescence resonance energy transfer.
Selvin PR., Nat. Struct. Biol. 7(9), 2000
PMID: 10966639
Fluorescence quenching of dyes by tryptophan: interactions at atomic detail from combination of experiment and computer simulation.
Vaiana AC, Neuweiler H, Schulz A, Wolfrum J, Sauer M, Smith JC., J. Am. Chem. Soc. 125(47), 2003
PMID: 14624606
A close look at fluorescence quenching of organic dyes by tryptophan.
Doose S, Neuweiler H, Sauer M., Chemphyschem 6(11), 2005
PMID: 16224752

Jelley, Nature 138(), 1936

Scheibe, Angew. Chem. 50(), 1937

Seidel, J. Phys. Chem. 100(), 1996
Inter- and intramolecular fluorescence quenching of organic dyes by tryptophan.
Marme N, Knemeyer JP, Sauer M, Wolfrum J., Bioconjug. Chem. 14(6), 2003
PMID: 14624626

Heinlein, J. Phys. Chem. B 107(), 2003

Sauer, Chem. Phys. Lett. 284(), 1998
Probes for detection of specific DNA sequences at the single-molecule level.
Knemeyer JP, Marme N, Sauer M., Anal. Chem. 72(16), 2000
PMID: 10959954
Conformational transitions monitored for single molecules in solution.
Edman L, Mets U, Rigler R., Proc. Natl. Acad. Sci. U.S.A. 93(13), 1996
PMID: 8692883
Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy.
Eggeling C, Fries JR, Brand L, Gunther R, Seidel CA., Proc. Natl. Acad. Sci. U.S.A. 95(4), 1998
PMID: 9465054

Wallace, J. Phys. Chem. B 104(), 2000
Kinetics of conformational fluctuations in DNA hairpin-loops.
Bonnet G, Krichevsky O, Libchaber A., Proc. Natl. Acad. Sci. U.S.A. 95(15), 1998
PMID: 9671724
Dynamics of unfolded polypeptide chains in crowded environment studied by fluorescence correlation spectroscopy.
Neuweiler H, Lollmann M, Doose S, Sauer M., J. Mol. Biol. 365(3), 2006
PMID: 17084857
Measurement of submicrosecond intramolecular contact formation in peptides at the single-molecule level.
Neuweiler H, Schulz A, Bohmer M, Enderlein J, Sauer M., J. Am. Chem. Soc. 125(18), 2003
PMID: 12720444
Protein conformational dynamics probed by single-molecule electron transfer.
Yang H, Luo G, Karnchanaphanurach P, Louie TM, Rech I, Cova S, Xun L, Xie XS., Science 302(5643), 2003
PMID: 14551431
The kinetics of conformational fluctuations in an unfolded protein measured by fluorescence methods.
Chattopadhyay K, Elson EL, Frieden C., Proc. Natl. Acad. Sci. U.S.A. 102(7), 2005
PMID: 15701687
Ultrafast dynamics of protein collapse from single-molecule photon statistics.
Nettels D, Gopich IV, Hoffmann A, Schuler B., Proc. Natl. Acad. Sci. U.S.A. 104(8), 2007
PMID: 17301233
A microscopic view of miniprotein folding: enhanced folding efficiency through formation of an intermediate.
Neuweiler H, Doose S, Sauer M., Proc. Natl. Acad. Sci. U.S.A. 102(46), 2005
PMID: 16269542

Marcus, Biochim. Biophys. Acta Rev. Bioenerg. 811(), 1985
Electron transfer in proteins.
Gray HB, Winkler JR., Annu. Rev. Biochem. 65(), 1996
PMID: 8811189
Signaling Recognition Events with Fluorescent Sensors and Switches.
de Silva AP, Gunaratne HQ, Gunnlaugsson T, Huxley AJ, McCoy CP, Rademacher JT, Rice TE., Chem. Rev. 97(5), 1997
PMID: 11851458

Weller, Z. Phys. Chem. 133(), 1982

Kavarnos, Chem. Rev. 86(), 1986

Kavarnos, 1993

Rehm, Isr. J. Chem. 8(), 1970

Adams, J. Phys. Chem. B 107(), 2003

Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base.
Torimura M, Kurata S, Yamada K, Yokomaku T, Kamagata Y, Kanagawa T, Kurane R., Anal Sci 17(1), 2001
PMID: 11993654

Widengren, Chem. Phys. 216(), 1997
Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes.
Nazarenko I, Pires R, Lowe B, Obaidy M, Rashtchian A., Nucleic Acids Res. 30(9), 2002
PMID: 11972350
The initial step of DNA hairpin folding: a kinetic analysis using fluorescence correlation spectroscopy.
Kim J, Doose S, Neuweiler H, Sauer M., Nucleic Acids Res. 34(9), 2006
PMID: 16687657

Wagenknecht, J. Am. Chem. Soc. 122(), 2000

DeFelippis, J. Phys. Chem. 95(), 1991
Pulse radiolytic measurement of redox potentials: the tyrosine and tryptophan radicals.
DeFelippis MR, Murthy CP, Faraggi M, Klapper MH., Biochemistry 28(11), 1989
PMID: 2765513

Jovanovic, J. Phys. Chem. 90(), 1986

Wardman, J. Phys. Chem. Ref. Data 18(), 1989
Dynamics of photoinduced charge transfer and hole transport in synthetic DNA hairpins.
Lewis FD, Letsinger RL, Wasielewski MR., Acc. Chem. Res. 34(2), 2001
PMID: 11263874

Vogelsang, Angew. Chem. 120(), 2008

AUTHOR UNKNOWN, Angew. Chem. Int. Ed. 47(), 2008
The initial step of DNA hairpin folding: a kinetic analysis using fluorescence correlation spectroscopy.
Kim J, Doose S, Neuweiler H, Sauer M., Nucleic Acids Res. 34(9), 2006
PMID: 16687657
Fluorescence quenching of dyes by tryptophan: interactions at atomic detail from combination of experiment and computer simulation.
Vaiana AC, Neuweiler H, Schulz A, Wolfrum J, Sauer M, Smith JC., J. Am. Chem. Soc. 125(47), 2003
PMID: 14624606
Fluorescence correlation spectroscopy. II. An experimental realization.
Magde D, Elson EL, Webb WW., Biopolymers 13(1), 1974
PMID: 4818131

Rigler, Eur. Biophys. J. 22(), 1993
Biological and chemical applications of fluorescence correlation spectroscopy: a review.
Hess ST, Huang S, Heikal AA, Webb WW., Biochemistry 41(3), 2002
PMID: 11790090

Krichevsky, Rep. Prog. Phys. 65(), 2002
Thermodynamic analysis of biomolecular interactions.
Cooper A., Curr Opin Chem Biol 3(5), 1999
PMID: 10508661
Energetics of protein folding.
Baldwin RL., J. Mol. Biol. 371(2), 2007
PMID: 17582437
Orientational and dynamical heterogeneity of rhodamine 6G terminally attached to a DNA helix revealed by NMR and single-molecule fluorescence spectroscopy.
Neubauer H, Gaiko N, Berger S, Schaffer J, Eggeling C, Tuma J, Verdier L, Seidel CA, Griesinger C, Volkmer A., J. Am. Chem. Soc. 129(42), 2007
PMID: 17900110
Location of cyanine-3 on double-stranded DNA: importance for fluorescence resonance energy transfer studies.
Norman DG, Grainger RJ, Uhrin D, Lilley DM., Biochemistry 39(21), 2000
PMID: 10828944
Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids.
Iqbal A, Arslan S, Okumus B, Wilson TJ, Giraud G, Norman DG, Ha T, Lilley DM., Proc. Natl. Acad. Sci. U.S.A. 105(32), 2008
PMID: 18676615
The structure of cyanine 5 terminally attached to double-stranded DNA: implications for FRET studies.
Iqbal A, Wang L, Thompson KC, Lilley DM, Norman DG., Biochemistry 47(30), 2008
PMID: 18597488

Kapanidis, J. Chem. Phys. 117(), 2002

Szabo, J. Chem. Phys. 72(), 1980

Pastor, J. Chem. Phys. 105(), 1996

Wilemski, J. Chem. Phys. 60(), 1974

Portman, J. Chem. Phys. 118(), 2003
Critical chain length for polyproline-II structure formation in H-Gly-(Pro)n-OH.
Helbecque N, Loucheux-Lefebvre MH., Int. J. Pept. Protein Res. 19(1), 1982
PMID: 7118386
Effect of flexibility and cis residues in single-molecule FRET studies of polyproline.
Best RB, Merchant KA, Gopich IV, Schuler B, Bax A, Eaton WA., Proc. Natl. Acad. Sci. U.S.A. 104(48), 2007
PMID: 18029448
What fluorescence correlation spectroscopy can tell us about unfolded proteins.
Frieden C, Chattopadhyay K, Elson EL., Adv. Protein Chem. 62(), 2002
PMID: 12418102
Spectroscopic study and evaluation of red-absorbing fluorescent dyes.
Buschmann V, Weston KD, Sauer M., Bioconjug. Chem. 14(1), 2003
PMID: 12526709
Long-range electron transfer.
Gray HB, Winkler JR., Proc. Natl. Acad. Sci. U.S.A. 102(10), 2005
PMID: 15738403

Haas, Biopolymers 17(), 1978
Diffusion-limited contact formation in unfolded cytochrome c: estimating the maximum rate of protein folding.
Hagen SJ, Hofrichter J, Szabo A, Eaton WA., Proc. Natl. Acad. Sci. U.S.A. 93(21), 1996
PMID: 8876184
Dynamics of unfolded polypeptide chains as model for the earliest steps in protein folding.
Krieger F, Fierz B, Bieri O, Drewello M, Kiefhaber T., J. Mol. Biol. 332(1), 2003
PMID: 12946363

Lapidus, J. Phys. Chem. B 106(), 2002
Measuring the rate of intramolecular contact formation in polypeptides.
Lapidus LJ, Eaton WA, Hofrichter J., Proc. Natl. Acad. Sci. U.S.A. 97(13), 2000
PMID: 10860987

Huang, Angew. Chem. 115(), 2003

AUTHOR UNKNOWN, Angew. Chem. Int. Ed. 42(), 2003
Loop formation in unfolded polypeptide chains on the picoseconds to microseconds time scale.
Fierz B, Satzger H, Root C, Gilch P, Zinth W, Kiefhaber T., Proc. Natl. Acad. Sci. U.S.A. 104(7), 2007
PMID: 17284588
A backbone-based theory of protein folding.
Rose GD, Fleming PJ, Banavar JR, Maritan A., Proc. Natl. Acad. Sci. U.S.A. 103(45), 2006
PMID: 17075053
Designing a 20-residue protein.
Neidigh JW, Fesinmeyer RM, Andersen NH., Nat. Struct. Biol. 9(6), 2002
PMID: 11979279
Smaller and faster: the 20-residue Trp-cage protein folds in 4 micros.
Qiu L, Pabit SA, Roitberg AE, Hagen SJ., J. Am. Chem. Soc. 124(44), 2002
PMID: 12405814

Doose, 2007
Changes in conformational dynamics of mRNA upon AtGRP7 binding studied by fluorescence correlation spectroscopy.
Schuttpelz M, Schoning JC, Doose S, Neuweiler H, Peters E, Staiger D, Sauer M., J. Am. Chem. Soc. 130(29), 2008
PMID: 18576621

Scheffler, Z. Phys. Chem. 219(), 2005

Neuweiler, Angew. Chem. 114(), 2002

AUTHOR UNKNOWN, Angew. Chem. Int. Ed. 41(), 2002
Fluorescence quenching: A tool for single-molecule protein-folding study.
Zhuang X, Ha T, Kim HD, Centner T, Labeit S, Chu S., Proc. Natl. Acad. Sci. U.S.A. 97(26), 2000
PMID: 11121030


Kasper, Proc. SPIE-Int. Soc. Opt. Eng. 6633(), 2007
New fluorescent dyes in the red region for biodiagnostics.
Sauer M, Han KT, Muller R, Nord S, Schulz A, Seeger S, Wolfrum J, Arden-Jacob J, Deltau G, Marx NJ, Zander C, Drexhage KH., J Fluoresc 5(3), 1995
PMID: 24226792

Steenken, J. Am. Chem. Soc. 119(), 1997
Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy.
Vogelsang J, Cordes T, Forthmann C, Steinhauer C, Tinnefeld P., Proc. Natl. Acad. Sci. U.S.A. 106(20), 2009
PMID: 19433792

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

PMID: 19475638
PubMed | Europe PMC

Suchen in

Google Scholar