Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction
Tschapalda K, Streitner N, Voß C, Flaschel E (2009)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84(1): 199-204.
Zeitschriftenaufsatz
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Autor*in
Tschapalda, Kirsten;
Streitner, Nadine;
Voß, CarstenUniBi;
Flaschel, ErwinUniBi
Einrichtung
Abstract / Bemerkung
The separation of structurally related impurities from pharmaceutical plasmid DNA by highly scalable purification techniques is a challenge for biochemical engineering. Next to RNA, proteins, and lipopolysaccharides, the chromosomal DNA of the plasmid replicating host has to be removed. Here, we describe the application of reverse micellar extraction for the separation of chromosomal from plasmid DNA. By applying different procedures for alkaline lysis, bacterial lysates with different amounts of chromosomal DNA were generated. A reverse micellar extraction step enabled us to deplete the concentration of this impurity below the required level of 50 mg g(-1) of plasmid DNA with almost complete plasmid recovery.
Stichworte
Reverse micelles;
Extraction;
Chromosomal DNA;
Plasmid production;
Alkaline lysis
Erscheinungsjahr
2009
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
84
Ausgabe
1
Seite(n)
199-204
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/1591533
Zitieren
Tschapalda K, Streitner N, Voß C, Flaschel E. Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2009;84(1):199-204.
Tschapalda, K., Streitner, N., Voß, C., & Flaschel, E. (2009). Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(1), 199-204. https://doi.org/10.1007/s00253-009-2088-3
Tschapalda, Kirsten, Streitner, Nadine, Voß, Carsten, and Flaschel, Erwin. 2009. “Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84 (1): 199-204.
Tschapalda, K., Streitner, N., Voß, C., and Flaschel, E. (2009). Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84, 199-204.
Tschapalda, K., et al., 2009. Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(1), p 199-204.
K. Tschapalda, et al., “Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 84, 2009, pp. 199-204.
Tschapalda, K., Streitner, N., Voß, C., Flaschel, E.: Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 84, 199-204 (2009).
Tschapalda, Kirsten, Streitner, Nadine, Voß, Carsten, and Flaschel, Erwin. “Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84.1 (2009): 199-204.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
2 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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PMID: 29018254
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PMID: 29018254
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PMID: 23831957
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PMID: 23831957
20 References
Daten bereitgestellt von Europe PubMed Central.
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Birnboim HC, Doly J., Nucleic Acids Res. 7(6), 1979
PMID: 388356
Birnboim HC, Doly J., Nucleic Acids Res. 7(6), 1979
PMID: 388356
Mechanical disruption of Escherichia coli for plasmid recovery.
Carlson A, Signs M, Liermann L, Boor R, Jem KJ., Biotechnol. Bioeng. 48(4), 1995
PMID: 18623491
Carlson A, Signs M, Liermann L, Boor R, Jem KJ., Biotechnol. Bioeng. 48(4), 1995
PMID: 18623491
Alkaline-cell lysis through in-line static mixer reactor for the production of plasmid DNA for gene therapy.
Chamsart S, Karnjanasorn T., Biotechnol. Bioeng. 96(3), 2007
PMID: 16917953
Chamsart S, Karnjanasorn T., Biotechnol. Bioeng. 96(3), 2007
PMID: 16917953
The impact of fluid-dynamic-generated stresses on chDNA and pDNA stability during alkaline cell lysis for gene therapy products.
Chamsart S, Patel H, Hanak JA, Hitchcock AG, Nienow AW., Biotechnol. Bioeng. 75(4), 2001
PMID: 11668438
Chamsart S, Patel H, Hanak JA, Hitchcock AG, Nienow AW., Biotechnol. Bioeng. 75(4), 2001
PMID: 11668438
M, J Chem Eng Jpn 32(), 1999
TA, 1989
Reverse micellar extraction for downstream processing of proteins/enzymes.
Krishna SH, Srinivas ND, Raghavarao KS, Karanth NG., Adv. Biochem. Eng. Biotechnol. 75(), 2002
PMID: 11787493
Krishna SH, Srinivas ND, Raghavarao KS, Karanth NG., Adv. Biochem. Eng. Biotechnol. 75(), 2002
PMID: 11787493
R, J Chromatogr B 784(), 2003
Biochemical engineering approaches to the challenges of producing pure plasmid DNA.
Levy MS, O'Kennedy RD, Ayazi-Shamlou P, Dunnill P., Trends Biotechnol. 18(7), 2000
PMID: 10856925
Levy MS, O'Kennedy RD, Ayazi-Shamlou P, Dunnill P., Trends Biotechnol. 18(7), 2000
PMID: 10856925
Degradation of supercoiled plasmid DNA within a capillary device.
Meacle FJ, Zhang H, Papantoniou I, Ward JM, Titchener-Hooker NJ, Hoare M., Biotechnol. Bioeng. 97(5), 2007
PMID: 17115451
Meacle FJ, Zhang H, Papantoniou I, Ward JM, Titchener-Hooker NJ, Hoare M., Biotechnol. Bioeng. 97(5), 2007
PMID: 17115451
Proposal for a better integration of bacterial lysis into the production of plasmid DNA at large scale.
O'Mahony K, Freitag R, Hilbrig F, Muller P, Schumacher I., J. Biotechnol. 119(2), 2005
PMID: 15993505
O'Mahony K, Freitag R, Hilbrig F, Muller P, Schumacher I., J. Biotechnol. 119(2), 2005
PMID: 15993505
Integration of bacteria capture via filtration and in situ lysis for recovery of plasmid DNA under industry-compatible conditions.
O'Mahony K, Freitag R, Hilbrig F, Schumacher I, Muller P., Biotechnol. Prog. 23(4), 2007
PMID: 17628077
O'Mahony K, Freitag R, Hilbrig F, Schumacher I, Muller P., Biotechnol. Prog. 23(4), 2007
PMID: 17628077
J, 1989
Reverse micellar extraction systems for the purification of pharmaceutical grade plasmid DNA.
Streitner N, Voss C, Flaschel E., J. Biotechnol. 131(2), 2007
PMID: 17673324
Streitner N, Voss C, Flaschel E., J. Biotechnol. 131(2), 2007
PMID: 17673324
N, Chem Ing Tech 80(), 2008
C, Biotechnol Annu Rev 13C(), 2007
C, Chem Eng Technol 31(), 2008
C, 2005
Production of supercoiled multimeric plasmid DNA for biopharmaceutical application.
Voss C, Schmidt T, Schleef M, Friehs K, Flaschel E., J. Biotechnol. 105(3), 2003
PMID: 14580792
Voss C, Schmidt T, Schleef M, Friehs K, Flaschel E., J. Biotechnol. 105(3), 2003
PMID: 14580792
Prediction of shear damage of plasmid DNA in pump and centrifuge operations using an ultra scale-down device.
Zhang H, Kong S, Booth A, Boushaba R, Levy MS, Hoare M., Biotechnol. Prog. 23(4), 2007
PMID: 17672520
Zhang H, Kong S, Booth A, Boushaba R, Levy MS, Hoare M., Biotechnol. Prog. 23(4), 2007
PMID: 17672520
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