Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction

Tschapalda K, Streitner N, Voß C, Flaschel E (2009)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84(1): 199-204.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor/in
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Abstract / Bemerkung
The separation of structurally related impurities from pharmaceutical plasmid DNA by highly scalable purification techniques is a challenge for biochemical engineering. Next to RNA, proteins, and lipopolysaccharides, the chromosomal DNA of the plasmid replicating host has to be removed. Here, we describe the application of reverse micellar extraction for the separation of chromosomal from plasmid DNA. By applying different procedures for alkaline lysis, bacterial lysates with different amounts of chromosomal DNA were generated. A reverse micellar extraction step enabled us to deplete the concentration of this impurity below the required level of 50 mg g(-1) of plasmid DNA with almost complete plasmid recovery.
Stichworte
Reverse micelles; Extraction; Chromosomal DNA; Plasmid production; Alkaline lysis
Erscheinungsjahr
2009
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
84
Ausgabe
1
Seite(n)
199-204
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/1591533

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Tschapalda K, Streitner N, Voß C, Flaschel E. Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2009;84(1):199-204.
Tschapalda, K., Streitner, N., Voß, C., & Flaschel, E. (2009). Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(1), 199-204. doi:10.1007/s00253-009-2088-3
Tschapalda, K., Streitner, N., Voß, C., and Flaschel, E. (2009). Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84, 199-204.
Tschapalda, K., et al., 2009. Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(1), p 199-204.
K. Tschapalda, et al., “Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 84, 2009, pp. 199-204.
Tschapalda, K., Streitner, N., Voß, C., Flaschel, E.: Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 84, 199-204 (2009).
Tschapalda, Kirsten, Streitner, Nadine, Voß, Carsten, and Flaschel, Erwin. “Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 84.1 (2009): 199-204.

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