The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion

Matthew T, Zhou W, Rupprecht J, Lim L, Thomas-Hall SR, Doebbe A, Kruse O, Hankamer B, Marx UC, Smith SM, Schenk PM (2009)
JOURNAL OF BIOLOGICAL CHEMISTRY 284(35): 23415-23425.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ; ; ; ; ; ;
Abstract / Bemerkung
The metabolome of the model species Chlamydomonas reinhardtii has been analyzed during 120 h of sulfur depletion to induce anaerobic hydrogen (H-2) production, using NMR spectroscopy, gas chromatography coupled to mass spectrometry, and TLC. The results indicate that these unicellular green algae consume freshly supplied acetate in the medium to accumulate energy reserves during the first 24 h of sulfur depletion. In addition to the previously reported accumulation of starch, large amounts of triacylglycerides were deposited in the cells. During the early 24- to 72-h time period fermentative energy metabolism lowered the pH, H-2 was produced, and amino acid levels generally increased. In the final phase from 72 to 120 h, metabolism slowed down leading to a stabilization of pH, even though some starch and most triacylglycerides remained. We conclude that H-2 production does not slow down due to depletion of energy reserves but rather due to loss of essential functions resulting from sulfur depletion or due to a build-up of the toxic fermentative products formate and ethanol.
Erscheinungsjahr
2009
Zeitschriftentitel
JOURNAL OF BIOLOGICAL CHEMISTRY
Band
284
Ausgabe
35
Seite(n)
23415-23425
ISSN
0021-9258
eISSN
1083-351X
Page URI
https://pub.uni-bielefeld.de/record/1591125

Zitieren

Matthew T, Zhou W, Rupprecht J, et al. The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY. 2009;284(35):23415-23425.
Matthew, T., Zhou, W., Rupprecht, J., Lim, L., Thomas-Hall, S. R., Doebbe, A., Kruse, O., et al. (2009). The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY, 284(35), 23415-23425. doi:10.1074/jbc.M109.003541
Matthew, T., Zhou, W., Rupprecht, J., Lim, L., Thomas-Hall, S. R., Doebbe, A., Kruse, O., Hankamer, B., Marx, U. C., Smith, S. M., et al. (2009). The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY 284, 23415-23425.
Matthew, T., et al., 2009. The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY, 284(35), p 23415-23425.
T. Matthew, et al., “The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 284, 2009, pp. 23415-23425.
Matthew, T., Zhou, W., Rupprecht, J., Lim, L., Thomas-Hall, S.R., Doebbe, A., Kruse, O., Hankamer, B., Marx, U.C., Smith, S.M., Schenk, P.M.: The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion. JOURNAL OF BIOLOGICAL CHEMISTRY. 284, 23415-23425 (2009).
Matthew, Timmins, Zhou, Wenxu, Rupprecht, Jens, Lim, Lysha, Thomas-Hall, Skye R., Doebbe, Anja, Kruse, Olaf, Hankamer, Ben, Marx, Ute C., Smith, Steven M., and Schenk, Peer M. “The Metabolome of Chlamydomonas reinhardtii following Induction of Anaerobic H-2 Production by Sulfur Depletion”. JOURNAL OF BIOLOGICAL CHEMISTRY 284.35 (2009): 23415-23425.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 19478077
PubMed | Europe PMC

Suchen in

Google Scholar