Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli

Spexard M, Beshay U, Risse JM, Miksch G, Flaschel E (2010)
BIOTECHNOLOGY LETTERS 32(2): 243-248.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The extracellular production of a hybrid bacterial beta-glucanase using Escherichia coli was studied by using combinations of promoters of varying strength for both a beta-glucanase as the target protein and the Kil protein as the releasing factor. Four strains with different combinations of promoter strengths were cultivated in shake-flasks on four different media to assess the cross-influence of promoter and medium in a general manner. Promoters were taken from natural as well as synthetic sequences known to exhibit either weak or strong promoter strength. By far the highest extracellular glucanase activity (> 200 U ml(-1)) was achieved when a strain harbouring the kil gene under control of a strong synthetic stationary-phase promoter and the glucanase gene under control of a strong synthetic constitutive promoter was cultivated on a complex medium mainly composed of casein peptone, yeast extract, and glycerol.
Stichworte
beta-Glucanase; Complex; E. coli; Extracellular enzyme; Fermentation; and synthetic media
Erscheinungsjahr
2010
Zeitschriftentitel
BIOTECHNOLOGY LETTERS
Band
32
Ausgabe
2
Seite(n)
243-248
ISSN
0141-5492
eISSN
1573-6776
Page URI
https://pub.uni-bielefeld.de/record/1588919

Zitieren

Spexard M, Beshay U, Risse JM, Miksch G, Flaschel E. Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS. 2010;32(2):243-248.
Spexard, M., Beshay, U., Risse, J. M., Miksch, G., & Flaschel, E. (2010). Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS, 32(2), 243-248. doi:10.1007/s10529-009-0133-z
Spexard, M., Beshay, U., Risse, J. M., Miksch, G., and Flaschel, E. (2010). Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS 32, 243-248.
Spexard, M., et al., 2010. Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS, 32(2), p 243-248.
M. Spexard, et al., “Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli”, BIOTECHNOLOGY LETTERS, vol. 32, 2010, pp. 243-248.
Spexard, M., Beshay, U., Risse, J.M., Miksch, G., Flaschel, E.: Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli. BIOTECHNOLOGY LETTERS. 32, 243-248 (2010).
Spexard, Meike, Beshay, Usama, Risse, Joe Max, Miksch, Gerd, and Flaschel, Erwin. “Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli”. BIOTECHNOLOGY LETTERS 32.2 (2010): 243-248.

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