Induction of DNA Breakage in U937 Cells by Oxazaphosphorines

Mazur L, Opydo-Chanek M, Stojak M, Baran J, Niemeyer, IIT-GmbH U (2010)
FOLIA BIOLOGICA-KRAKOW 58(1): 15-20.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Oxazaphosphorines are a class of DNA alkylating agents. The aim of the present study was to compare the possible influence of three new generation oxazaphosphorines, D-17272 (mafosfamide cyclohexylamine salt), D-18864 (4-hydro-peroxy-cyclophosphamide), and D-19575 (glufosfamide, P-D-glucose-isophosphoramide mustard) on DNA damage induction in the human histiocytic lymphoma U937 cells. The flow cytometry APO-BRDU (TM) assay, based on the TUNEL method, was used for the in situ detection of DNA strand breaks. After exposure of U937 cells to the oxazaphosphorines, the patterns of temporary changes in the frequency of TUNEL positive U937 cells, expressing DNA breakage, were determined. The effects of the oxazaphosphorines on U937 cells were dependent on the agent tested and its dose, and the time intervals after the drug application. The different potential of D-I 7272, D-18864 and D-19575 to induce DNA strand breakage in the human histiocytic lymphoma U937 cells was shown.
Stichworte
Oxazaphosphorines; DNA strand breaks; TUNEL technique; U937 cells
Erscheinungsjahr
2010
Zeitschriftentitel
FOLIA BIOLOGICA-KRAKOW
Band
58
Ausgabe
1
Seite(n)
15-20
ISSN
0015-5497
eISSN
1734-9168
Page URI
https://pub.uni-bielefeld.de/record/1588806

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Mazur L, Opydo-Chanek M, Stojak M, Baran J, Niemeyer, IIT-GmbH U. Induction of DNA Breakage in U937 Cells by Oxazaphosphorines. FOLIA BIOLOGICA-KRAKOW. 2010;58(1):15-20.
Mazur, L., Opydo-Chanek, M., Stojak, M., Baran, J., & Niemeyer, IIT-GmbH, U. (2010). Induction of DNA Breakage in U937 Cells by Oxazaphosphorines. FOLIA BIOLOGICA-KRAKOW, 58(1), 15-20. doi:10.3409/fb58_1-2.15-20
Mazur, L., Opydo-Chanek, M., Stojak, M., Baran, J., and Niemeyer, IIT-GmbH, U. (2010). Induction of DNA Breakage in U937 Cells by Oxazaphosphorines. FOLIA BIOLOGICA-KRAKOW 58, 15-20.
Mazur, L., et al., 2010. Induction of DNA Breakage in U937 Cells by Oxazaphosphorines. FOLIA BIOLOGICA-KRAKOW, 58(1), p 15-20.
L. Mazur, et al., “Induction of DNA Breakage in U937 Cells by Oxazaphosphorines”, FOLIA BIOLOGICA-KRAKOW, vol. 58, 2010, pp. 15-20.
Mazur, L., Opydo-Chanek, M., Stojak, M., Baran, J., Niemeyer, IIT-GmbH, U.: Induction of DNA Breakage in U937 Cells by Oxazaphosphorines. FOLIA BIOLOGICA-KRAKOW. 58, 15-20 (2010).
Mazur, Lidia, Opydo-Chanek, Malgorzata, Stojak, Marta, Baran, Jaroslaw, and Niemeyer, IIT-GmbH, Ulf. “Induction of DNA Breakage in U937 Cells by Oxazaphosphorines”. FOLIA BIOLOGICA-KRAKOW 58.1 (2010): 15-20.

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