An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR

van der Star WRL, Dijkema C, de Waard P, Picioreanu C, Strous M, van Loosdrecht MCM (2010)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 86(1): 311-317.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The cytoplasm of anaerobic ammonium oxidizing (anammox) bacteria consists of three compartments separated by membranes. It has been suggested that a proton motive force may be generated over the membrane of the innermost compartment, the "anammoxosome". P-31 nuclear magnetic resonance (NMR) spectroscopy was employed to investigate intracellular pH differences in the anammox bacterium Kuenenia stuttgartiensis. With in vivo NMR, spectra were recorded of active, highly concentrated suspensions of K. stuttgartiensis in a wide-bore NMR tube. At different external pH values, two stable and distinct phosphate peaks were apparent in the recorded spectra. These peaks were equivalent with pH values of 7.3 and 6.3 and suggested the presence of a proton motive force over an intracytoplasmic membrane in K. stuttgartiensis. This study provides for the second time-after discovery of acidocalcisome-like compartments in Agrobacterium tumefaciens-evidence for an intracytoplasmic pH gradient in a chemotrophic prokaryotic cell.
Stichworte
Internal pH; In vivo NMR; Nuclear; magnetic resonance spectroscopy; P-31 NMR; Anammox; Kuenenia stuttgartiensis
Erscheinungsjahr
2010
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
86
Ausgabe
1
Seite(n)
311-317
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/1588560

Zitieren

van der Star WRL, Dijkema C, de Waard P, Picioreanu C, Strous M, van Loosdrecht MCM. An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2010;86(1):311-317.
van der Star, W. R. L., Dijkema, C., de Waard, P., Picioreanu, C., Strous, M., & van Loosdrecht, M. C. M. (2010). An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 86(1), 311-317. doi:10.1007/s00253-009-2309-9
van der Star, W. R. L., Dijkema, C., de Waard, P., Picioreanu, C., Strous, M., and van Loosdrecht, M. C. M. (2010). An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 86, 311-317.
van der Star, W.R.L., et al., 2010. An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 86(1), p 311-317.
W.R.L. van der Star, et al., “An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 86, 2010, pp. 311-317.
van der Star, W.R.L., Dijkema, C., de Waard, P., Picioreanu, C., Strous, M., van Loosdrecht, M.C.M.: An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 86, 311-317 (2010).
van der Star, Wouter R. L., Dijkema, Cor, de Waard, Pieter, Picioreanu, Cristian, Strous, Marc, and van Loosdrecht, Mark C. M. “An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 86.1 (2010): 311-317.

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