T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants

Uelker B, Li Y, Rosso MG, Logemann E, Somssich IE, Weisshaar B (2008)
Nature Biotechnology 26(9): 1015-1017.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Besides the well-documented integration of DNA flanked by the transfer DNA borders, occasional insertion of fragments from the tumor-inducing plasmid into plant genomes has also been reported during Agrobacterium tumefaciens-mediated transformation. We demonstrate that large (up to approximately 18 kb) gene-bearing fragments of Agrobacterium chromosomal DNA (AchrDNA) can be integrated into Arabidopsis thaliana genomic DNA during transformation. One in every 250 transgenic plants may carry AchrDNA fragments. This has implications for horizontal gene transfer and indicates a need for greater scrutiny of transgenic plants for undesired bacterial DNA.
Nature Biotechnology


Uelker B, Li Y, Rosso MG, Logemann E, Somssich IE, Weisshaar B. T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology. 2008;26(9):1015-1017.
Uelker, B., Li, Y., Rosso, M. G., Logemann, E., Somssich, I. E., & Weisshaar, B. (2008). T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology, 26(9), 1015-1017. doi:10.1038/nbt.1491
Uelker, B., Li, Y., Rosso, M. G., Logemann, E., Somssich, I. E., and Weisshaar, B. (2008). T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology 26, 1015-1017.
Uelker, B., et al., 2008. T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology, 26(9), p 1015-1017.
B. Uelker, et al., “T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants”, Nature Biotechnology, vol. 26, 2008, pp. 1015-1017.
Uelker, B., Li, Y., Rosso, M.G., Logemann, E., Somssich, I.E., Weisshaar, B.: T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants. Nature Biotechnology. 26, 1015-1017 (2008).
Uelker, Bekir, Li, Yong, Rosso, Mario G., Logemann, Elke, Somssich, Imre E., and Weisshaar, Bernd. “T-DNA-mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants”. Nature Biotechnology 26.9 (2008): 1015-1017.

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