Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p

Neubert C, Graham LA, Black-Maier EW, Coonrod EM, Liu T-Y, Stierhof Y-D, Seidel T, Stevens TH, Schumacher K (2008)
Traffic 9(10): 1618-1628.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Neubert, Christoph; Graham, Laurie A.; Black-Maier, Eric W.; Coonrod, Emily M.; Liu, Tzu-Yin; Stierhof, York-Dieter; Seidel, ThorstenUniBi; Stevens, Tom H.; Schumacher, Karin
Abstract / Bemerkung
How individual protein subunits assemble into the higher order structure of a protein complex is not well understood. Four proteins dedicated to the assembly of the V-0 subcomplex of the V-adenosine triphosphatase (V-ATPase) in the endoplasmic reticulum (ER) have been identified in yeast, but their precise mode of molecular action remains to be identified. In contrast to the highly conserved subunits of the V-ATPase, orthologs of the yeast assembly factors are not easily identified based on sequence similarity. We show in this study that two ER-localized Arabidopsis proteins that share only 25% sequence identity with Vma21p can functionally replace this yeast assembly factor. Loss of AtVMA21a function in RNA interference seedlings caused impaired cell expansion and changes in Golgi morphology characteristic for plants with reduced V-ATPase activity, and we therefore conclude that AtVMA21a is the first V-ATPase assembly factor identified in a multicellular eukaryote. Moreover, VMA21p acts as a dedicated ER escort chaperone, a class of substrate-specific accessory proteins so far not identified in higher plants.
assembly factor; V-ATPase; protein complex; ER export; Arabidopsis
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Neubert C, Graham LA, Black-Maier EW, et al. Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p. Traffic. 2008;9(10):1618-1628.
Neubert, C., Graham, L. A., Black-Maier, E. W., Coonrod, E. M., Liu, T. - Y., Stierhof, Y. - D., Seidel, T., et al. (2008). Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p. Traffic, 9(10), 1618-1628.
Neubert, C., Graham, L. A., Black-Maier, E. W., Coonrod, E. M., Liu, T. - Y., Stierhof, Y. - D., Seidel, T., Stevens, T. H., and Schumacher, K. (2008). Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p. Traffic 9, 1618-1628.
Neubert, C., et al., 2008. Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p. Traffic, 9(10), p 1618-1628.
C. Neubert, et al., “Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p”, Traffic, vol. 9, 2008, pp. 1618-1628.
Neubert, C., Graham, L.A., Black-Maier, E.W., Coonrod, E.M., Liu, T.-Y., Stierhof, Y.-D., Seidel, T., Stevens, T.H., Schumacher, K.: Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p. Traffic. 9, 1618-1628 (2008).
Neubert, Christoph, Graham, Laurie A., Black-Maier, Eric W., Coonrod, Emily M., Liu, Tzu-Yin, Stierhof, York-Dieter, Seidel, Thorsten, Stevens, Tom H., and Schumacher, Karin. “Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p”. Traffic 9.10 (2008): 1618-1628.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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PMID: 29473670
PDMP induces rapid changes in vacuole morphology in Arabidopsis root cells.
Krüger F, Krebs M, Viotti C, Langhans M, Schumacher K, Robinson DG., J Exp Bot 64(2), 2013
PMID: 23230024
The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis.
Viotti C, Krüger F, Krebs M, Neubert C, Fink F, Lupanga U, Scheuring D, Boutté Y, Frescatada-Rosa M, Wolfenstetter S, Sauer N, Hillmer S, Grebe M, Schumacher K., Plant Cell 25(9), 2013
PMID: 24014545
Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein.
Liljegren SJ, Leslie ME, Darnielle L, Lewis MW, Taylor SM, Luo R, Geldner N, Chory J, Randazzo PA, Yanofsky MF, Ecker JR., Development 136(11), 2009
PMID: 19429787
Voa1p functions in V-ATPase assembly in the yeast endoplasmic reticulum.
Ryan M, Graham LA, Stevens TH., Mol Biol Cell 19(12), 2008
PMID: 18799613

34 References

Daten bereitgestellt von Europe PubMed Central.

VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae.
Hirata R, Umemoto N, Ho MN, Ohya Y, Stevens TH, Anraku Y., J. Biol. Chem. 268(2), 1993
PMID: 8419376
The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation.
Hajdukiewicz P, Svab Z, Maliga P., Plant Mol. Biol. 25(6), 1994
PMID: 7919218
Resolution of subunit interactions and cytoplasmic subcomplexes of the yeast vacuolar proton-translocating ATPase.
Tomashek JJ, Sonnenburg JL, Artimovich JM, Klionsky DJ., J. Biol. Chem. 271(17), 1996
PMID: 8626613
Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae.
Wach A, Brachat A, Alberti-Segui C, Rebischung C, Philippsen P., Yeast 13(11), 1997
PMID: 9290211
Structure, function and regulation of the vacuolar (H+)-ATPase.
Stevens TH, Forgac M., Annu. Rev. Cell Dev. Biol. 13(), 1997
PMID: 9442887
Essential role of the V-ATPase in male gametophyte development.
Dettmer J, Schubert D, Calvo-Weimar O, Stierhof YD, Schmidt R, Schumacher K., Plant J. 41(1), 2005
PMID: 15610354
Arabidopsis vacuolar H-ATPase subunit E isoform 1 is required for Golgi organization and vacuole function in embryogenesis.
Strompen G, Dettmer J, Stierhof YD, Schumacher K, Jurgens G, Mayer U., Plant J. 41(1), 2005
PMID: 15610355
Mapping of C-termini of V-ATPase subunits by in vivo-FRET measurements.
Seidel T, Golldack D, Dietz KJ., FEBS Lett. 579(20), 2005
PMID: 16061227
Vacuolar H+-ATPase activity is required for endocytic and secretory trafficking in Arabidopsis.
Dettmer J, Hong-Hermesdorf A, Stierhof YD, Schumacher K., Plant Cell 18(3), 2006
PMID: 16461582
Vma9p (subunit e) is an integral membrane V0 subunit of the yeast V-ATPase.
Compton MA, Graham LA, Stevens TH., J. Biol. Chem. 281(22), 2006
PMID: 16569636
PKR1 encodes an assembly factor for the yeast V-type ATPase.
Davis-Kaplan SR, Compton MA, Flannery AR, Ward DM, Kaplan J, Stevens TH, Graham LA., J. Biol. Chem. 281(42), 2006
PMID: 16926153
Multicolor single-molecule spectroscopy with alternating laser excitation for the investigation of interactions and dynamics.
Ross J, Buschkamp P, Fetting D, Donnermeyer A, Roth CM, Tinnefeld P., J Phys Chem B 111(2), 2007
PMID: 17214479
Vacuolar ATPases: rotary proton pumps in physiology and pathophysiology.
Forgac M., Nat. Rev. Mol. Cell Biol. 8(11), 2007
PMID: 17912264
Reduced V-ATPase activity in the trans-Golgi network causes oxylipin-dependent hypocotyl growth Inhibition in Arabidopsis.
Brux A, Liu TY, Krebs M, Stierhof YD, Lohmann JU, Miersch O, Wasternack C, Schumacher K., Plant Cell 20(4), 2008
PMID: 18441211
Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-FRET analysis.
Seidel T, Schnitzer D, Golldack D, Sauer M, Dietz KJ., BMC Cell Biol. 9(), 2008
PMID: 18507826
The Arabidopsis det3 mutant reveals a central role for the vacuolar H(+)-ATPase in plant growth and development.
Schumacher K, Vafeados D, McCarthy M, Sze H, Wilkins T, Chory J., Genes Dev. 13(24), 1999
PMID: 10617574
Construct design for efficient, effective and high-throughput gene silencing in plants.
Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green AG, Waterhouse PM., Plant J. 27(6), 2001
PMID: 11576441
Characterization of the ethanol-inducible alc gene-expression system in Arabidopsis thaliana.
Roslan HA, Salter MG, Wood CD, White MR, Croft KP, Robson F, Coupland G, Doonan J, Laufs P, Tomsett AB, Caddick MX., Plant J. 28(2), 2001
PMID: 11722766
The vacuolar (H+)-ATPases--nature's most versatile proton pumps.
Nishi T, Forgac M., Nat. Rev. Mol. Cell Biol. 3(2), 2002
PMID: 11836511
A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase.
Sze H, Schumacher K, Muller ML, Padmanaban S, Taiz L., Trends Plant Sci. 7(4), 2002
PMID: 11950611
Studying yeast vacuoles.
Conibear E, Stevens TH., Meth. Enzymol. 351(), 2002
PMID: 12073360
Structure and assembly of the yeast V-ATPase.
Graham LA, Flannery AR, Stevens TH., J. Bioenerg. Biomembr. 35(4), 2003
PMID: 14635776
Role of Vma21p in assembly and transport of the yeast vacuolar ATPase.
Malkus P, Graham LA, Stevens TH, Schekman R., Mol. Biol. Cell 15(11), 2004
PMID: 15356264
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
Markwell MA, Haas SM, Bieber LL, Tolbert NE., Anal. Biochem. 87(1), 1978
PMID: 98070
STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p.
Manolson MF, Wu B, Proteau D, Taillon BE, Roberts BT, Hoyt MA, Jones EW., J. Biol. Chem. 269(19), 1994
PMID: 7514599


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