Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level

Ataka K, Heberle J (2008)
BIOCHEMICAL SOCIETY TRANSACTIONS 36(Part 5): 986-991.

Konferenzbeitrag | Veröffentlicht| Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor*in
Ataka, Kenichi; Heberle, Joachim
Abstract / Bemerkung
The present study surveys a novel approach to studies of membrane proteins whose catalytic action is driven by the redox potential or by the membrane potential. We introduce SEIRAS (surface-enhanced IR absorption spectroscopy) to probe a monolayer of membrane protein adhered to the surface of a gold electrode. SEIRAS renders high surface sensitivity by enhancing the signal of the adsorbed molecule by approximately two orders of magnitude. it is demonstrated that reaction-induced spectroscopy is applicable by recording IR differences of cytochrome c after stimulation by the electrical potential. The impact of the membrane potential on the function of a membrane protein is demonstrated by performing light-induced difference spectroscopy on a microbial rhodopsin (sensory rhodopsin II) under voltage-clamp conditions. The methodology presented opens new avenues to study the mechanism of electron-triggered and voltage-gated proteins at the level of single bonds. As many of these catalytic reactions are of vectorial nature, control on the orientation of the membrane protein is mandatory. Approaches are presented on how to specifically adhere photosynthetic and respiratory proteins to the electrode surface and reconstitute these membrane proteins in the lipid bilayer. Functionality of such biomimetic systems is assessed in situ by spectro-electrochemical methods.
Stichworte
surface-enhanced IR absorption spectroscopy (SEIRAS); vibrational; spectroscopy; ion transfer; membrane potential; gold surface; proton translocation
Erscheinungsjahr
2008
Band
36
Ausgabe
Part 5
Seite(n)
986-991
ISSN
0300-5127
Page URI
https://pub.uni-bielefeld.de/record/1585846

Zitieren

Ataka K, Heberle J. Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level. BIOCHEMICAL SOCIETY TRANSACTIONS. 2008;36(Part 5):986-991.
Ataka, K., & Heberle, J. (2008). Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level. BIOCHEMICAL SOCIETY TRANSACTIONS, 36(Part 5), 986-991. doi:10.1042/BST0360986
Ataka, K., and Heberle, J. (2008). Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level. BIOCHEMICAL SOCIETY TRANSACTIONS 36, 986-991.
Ataka, K., & Heberle, J., 2008. Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level. BIOCHEMICAL SOCIETY TRANSACTIONS, 36(Part 5), p 986-991.
K. Ataka and J. Heberle, “Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level”, BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 36, 2008, pp. 986-991.
Ataka, K., Heberle, J.: Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level. BIOCHEMICAL SOCIETY TRANSACTIONS. 36, 986-991 (2008).
Ataka, Kenichi, and Heberle, Joachim. “Bioenergetics at the gold surface: SEIRAS probes photosynthetic and respiratory reactions at the monolayer level”. BIOCHEMICAL SOCIETY TRANSACTIONS 36.Part 5 (2008): 986-991.

3 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Recent applications of ATR FTIR spectroscopy and imaging to proteins.
Glassford SE, Byrne B, Kazarian SG., Biochim Biophys Acta 1834(12), 2013
PMID: 23928299
How algae produce hydrogen--news from the photosynthetic hydrogenase.
Stripp ST, Happe T., Dalton Trans (45), 2009
PMID: 19904421

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 18793175
PubMed | Europe PMC

Suchen in

Google Scholar