Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein
Miksch G, Ryu S, Risse JM, Flaschel E (2008)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 81(2): 319-326.
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Einrichtung
Abstract / Bemerkung
Aiming to increase production of recombinant streptavidin in Escherichia coli, the effect of different leader sequences, different promoter strengths of the bacteriocin release protein (kil), host strain and medium composition on the expression and secretion into the medium was investigated. Expression vectors containing an expression or secretion unit were constructed with different combinations of leader sequence for the streptavidin gene and promoters for the kil gene and streptavidin gene. Results showed that a high-level extracellular production of streptavidin could be accomplished with E. coli BL21(DE3) by using the leader sequence of the phoA gene, a strong stationary-phase promoter for the kil gene and supplementation of the medium by glycine. Using a stationary-phase promoter for the expression of streptavidin had a negative effect.
Stichworte
Promoter;
Streptavidin;
Bacteriocin release protein;
Glycine;
Secretion;
Escherichia coli;
Leader sequence
Erscheinungsjahr
2008
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
81
Ausgabe
2
Seite(n)
319-326
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/1585617
Zitieren
Miksch G, Ryu S, Risse JM, Flaschel E. Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2008;81(2):319-326.
Miksch, G., Ryu, S., Risse, J. M., & Flaschel, E. (2008). Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(2), 319-326. https://doi.org/10.1007/s00253-008-1673-1
Miksch, Gerd, Ryu, Stella, Risse, Joe Max, and Flaschel, Erwin. 2008. “Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 81 (2): 319-326.
Miksch, G., Ryu, S., Risse, J. M., and Flaschel, E. (2008). Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 81, 319-326.
Miksch, G., et al., 2008. Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(2), p 319-326.
G. Miksch, et al., “Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 81, 2008, pp. 319-326.
Miksch, G., Ryu, S., Risse, J.M., Flaschel, E.: Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 81, 319-326 (2008).
Miksch, Gerd, Ryu, Stella, Risse, Joe Max, and Flaschel, Erwin. “Factors that influence the extracellular expression of streptavidin in Escherichia coli using a bacteriocin release protein”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 81.2 (2008): 319-326.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
6 Zitationen in Europe PMC
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