Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs

Nuss AM, Heroven AK, Waldmann B, Reinkensmeier J, Jarek M, Beckstette M, Dersch P (2015)
PLOS GENETICS 11(3): 26.

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One hallmark of pathogenic yersiniae is their ability to rapidly adjust their life-style and pathogenesis upon host entry. In order to capture the range, magnitude and complexity of the underlying gene control mechanisms we used comparative RNA-seq-based transcriptomic profiling of the enteric pathogen Y. pseudotuberculosis under environmental and infection-relevant conditions. We identified 1151 individual transcription start sites, multiple ribos-witch-like RNA elements, and a global set of antisense RNAs and previously unrecognized trans-acting RNAs. Taking advantage of these data, we revealed a temperature-induced and growth phase-dependent reprogramming of a large set of catabolic/energy production genes and uncovered the existence of a thermo-regulated 'acetate switch', which appear to prime the bacteria for growth in the digestive tract. To elucidate the regulatory architecture linking nutritional status to virulence we also refined the CRP regulon. We identified a massive remodelling of the CRP-controlled network in response to temperature and discovered CRP as a transcriptional master regulator of numerous conserved and newly identified non-coding RNAs which participate in this process. This finding highlights a novel level of complexity of the regulatory network in which the concerted action of transcriptional regulators and multiple non-coding RNAs under control of CRP adjusts the control of Yersinia fitness and virulence to the requirements of their environmental and virulent life-styles.
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Nuss AM, Heroven AK, Waldmann B, et al. Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs. PLOS GENETICS. 2015;11(3): 26.
Nuss, A. M., Heroven, A. K., Waldmann, B., Reinkensmeier, J., Jarek, M., Beckstette, M., & Dersch, P. (2015). Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs. PLOS GENETICS, 11(3), 26. doi:10.1371/journal.pgen.1005087
Nuss, A. M., Heroven, A. K., Waldmann, B., Reinkensmeier, J., Jarek, M., Beckstette, M., and Dersch, P. (2015). Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs. PLOS GENETICS 11:26.
Nuss, A.M., et al., 2015. Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs. PLOS GENETICS, 11(3): 26.
A.M. Nuss, et al., “Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs”, PLOS GENETICS, vol. 11, 2015, : 26.
Nuss, A.M., Heroven, A.K., Waldmann, B., Reinkensmeier, J., Jarek, M., Beckstette, M., Dersch, P.: Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs. PLOS GENETICS. 11, : 26 (2015).
Nuss, Aaron M., Heroven, Ann Kathrin, Waldmann, Barbara, Reinkensmeier, Jan, Jarek, Michael, Beckstette, Michael, and Dersch, Petra. “Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs”. PLOS GENETICS 11.3 (2015): 26.
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20 Citations in Europe PMC

Data provided by Europe PubMed Central.

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RNA Regulators: Formidable Modulators of Yersinia Virulence.
Nuss AM, Heroven AK, Dersch P., Trends Microbiol 25(1), 2017
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In vivo expression technology and 5' end mapping of the Borrelia burgdorferi transcriptome identify novel RNAs expressed during mammalian infection.
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Several Hfq-dependent alterations in physiology of Yersinia enterocolitica O:3 are mediated by derepression of the transcriptional regulator RovM.
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Tissue dual RNA-seq allows fast discovery of infection-specific functions and riboregulators shaping host-pathogen transcriptomes.
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Genome-wide mapping of transcription start sites yields novel insights into the primary transcriptome of Pseudomonas putida.
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PMID: 27111755
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PMID: 27298343
Increased plasmid copy number is essential for Yersinia T3SS function and virulence.
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The Conserved Dcw Gene Cluster of R. sphaeroides Is Preceded by an Uncommonly Extended 5' Leader Featuring the sRNA UpsM.
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Regulatory principles governing Salmonella and Yersinia virulence.
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Yersinia pestis and Yersinia pseudotuberculosis infection: a regulatory RNA perspective.
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