Multifocus structured illumination microscopy for fast volumetric super-resolution imaging

Abrahamsson S, Blom H, Agostinho A, Jans DC, Jost A, Müller M, Nilsson L, Bernhem K, Lambert TJ, Heintzmann R, Brismar H (2017)
BIOMEDICAL OPTICS EXPRESS 8(9): 4135-4140.

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We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in superresolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens. (C) 2017 Optical Society of America
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Abrahamsson S, Blom H, Agostinho A, et al. Multifocus structured illumination microscopy for fast volumetric super-resolution imaging. BIOMEDICAL OPTICS EXPRESS. 2017;8(9):4135-4140.
Abrahamsson, S., Blom, H., Agostinho, A., Jans, D. C., Jost, A., Müller, M., Nilsson, L., et al. (2017). Multifocus structured illumination microscopy for fast volumetric super-resolution imaging. BIOMEDICAL OPTICS EXPRESS, 8(9), 4135-4140. doi:10.1364/BOE.8.004135
Abrahamsson, S., Blom, H., Agostinho, A., Jans, D. C., Jost, A., Müller, M., Nilsson, L., Bernhem, K., Lambert, T. J., Heintzmann, R., et al. (2017). Multifocus structured illumination microscopy for fast volumetric super-resolution imaging. BIOMEDICAL OPTICS EXPRESS 8, 4135-4140.
Abrahamsson, S., et al., 2017. Multifocus structured illumination microscopy for fast volumetric super-resolution imaging. BIOMEDICAL OPTICS EXPRESS, 8(9), p 4135-4140.
S. Abrahamsson, et al., “Multifocus structured illumination microscopy for fast volumetric super-resolution imaging”, BIOMEDICAL OPTICS EXPRESS, vol. 8, 2017, pp. 4135-4140.
Abrahamsson, S., Blom, H., Agostinho, A., Jans, D.C., Jost, A., Müller, M., Nilsson, L., Bernhem, K., Lambert, T.J., Heintzmann, R., Brismar, H.: Multifocus structured illumination microscopy for fast volumetric super-resolution imaging. BIOMEDICAL OPTICS EXPRESS. 8, 4135-4140 (2017).
Abrahamsson, Sara, Blom, Hans, Agostinho, Ana, Jans, Daniel C., Jost, Aurelie, Müller, Marcel, Nilsson, Linnea, Bernhem, Kristoffer, Lambert, Talley J., Heintzmann, Rainer, and Brismar, Hjalmar. “Multifocus structured illumination microscopy for fast volumetric super-resolution imaging”. BIOMEDICAL OPTICS EXPRESS 8.9 (2017): 4135-4140.
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